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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFK428
Library VF
(Link to library)
Clone ID VFK428
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13202-1
Original site URL
Representative seq. ID VFK428E
(Link to Original site)
Representative DNA sequence 
>VFK428 (VFK428Q) /CSM/VF/VFK4-B/VFK428Q.Seq.d/
AGTAATAATAAAATCAAATAAAAAAAAATATAAAATGAGAGTATTATTATCATTAGTTGT
AATTTTATTCATTATAAATTCAGCCTTTGCTGTAAAGATTAATATTGGTCGTCCAACCAA
ATCACATAAAACTATCCATCACGAAACTTGGGTAGAAGAACAAACTGATCAATTCGATAA
TATTAAAGTTGGCCAATTATTAGGTTTCAAGAGAAGTCCAAACAGACCAAAACTTCAAAT
TAAATCTTATGATCCATTAGGAGTTCAAATTCCAACTTCTTTCAATGCTCAAACCAACTG
GCCAAATTGTACTACCATTAGTCAGATTCAAAATCAAGCAAGATGTGGTTCTTGTTGGGC
TTTTGGTGCTACAGAATCAGCTACTGATCGTTTATGTATTCACAATAATGAAAATGTTCA
ATTAAGTTTTATGGATATGGTTACTTGTGATGAAACTGATAACGGTTGTGAAGGTGGTGA
TGCCTTCTCTGCATGGAATTGGTTAAGAAAGCAAGGTGCTGTATCAGAAGAATGTCTTCC
ATATACAATTCCAACTTGTCCACCAGCTCAACAACCATGTTTAAATTTTGTCAACACTCC
ATCATGTACTAAAGAGTGTCAATCAAATTCCTCTTTAATTTATTCTCAAGACAAACATAA
AATGGCTAAAATTTATTCTTTCGACTCTGATGAAGCAATCATGCAAGAAATTGTTACTAA
TGGTCCAGTCGAAGCTTGTTTCACTGTCTTTGAAGATTTCCTTGCTTACAAATCTGGTGT
TTACGTCCACACAACTGGTAAAGATTTAGGTGGTCACTGTGTTAAACTCGTTGGTTTCGG
TACCTTAAATGGTGTTNATTACTATGCCGCTAACAACCAATGGACAACTTCAAGGGGTNA
TAATGGAACTTTCTAATCAAACGTGGTGATTGCGGTATCTCTATGACGTTGT
sequence update 2001.11.24
Translated Amino Acid sequence 
VIIKSNKKKYKMRVLLSLVVILFIINSAFAVKINIGRPTKSHKTIHHETWVEEQTDQFDN
IKVGQLLGFKRSPNRPKLQIKSYDPLGVQIPTSFNAQTNWPNCTTISQIQNQARCGSCWA
FGATESATDRLCIHNNENVQLSFMDMVTCDETDNGCEGGDAFSAWNWLRKQGAVSEECLP
YTIPTCPPAQQPCLNFVNTPSCTKECQSNSSLIYSQDKHKMAKIYSFDSDEAIMQEIVTN
GPVEACFTVFEDFLAYKSGVYVHTTGKDLGGHCVKLVGFGTLNGVXYYAANNQWTTSRGX
NGTF*snvviavsl*rc
Translated Amino Acid sequence (All Frames) 
Frame A:
snnkik*kki*nesiiiiscnfihykfslcckd*ywssnqit*nypsrnlgrrtn*sir*
y*swpiirfqekskqtktsn*il*sirssnsnffqcsnqlaklyyh*sdsksskmwfllg
fwcyrisy*sfmysq**kcsikfygygyl**n**rl*rw*cllcmelvkkarccirrmss
iynsnlstssttmfkfcqhsimy*rvsikflfnlfsrqt*ng*nlffrl**snharncy*
wssrslfhcl*rfpclqiwclrphnw*rfrwslc*trwfrylkwcxllcr*qpmdnfkgx
*wnflikrgdcgismtl


Frame B:
VIIKSNKKKYKMRVLLSLVVILFIINSAFAVKINIGRPTKSHKTIHHETWVEEQTDQFDN
IKVGQLLGFKRSPNRPKLQIKSYDPLGVQIPTSFNAQTNWPNCTTISQIQNQARCGSCWA
FGATESATDRLCIHNNENVQLSFMDMVTCDETDNGCEGGDAFSAWNWLRKQGAVSEECLP
YTIPTCPPAQQPCLNFVNTPSCTKECQSNSSLIYSQDKHKMAKIYSFDSDEAIMQEIVTN
GPVEACFTVFEDFLAYKSGVYVHTTGKDLGGHCVKLVGFGTLNGVXYYAANNQWTTSRGX
NGTF*snvviavsl*rc


Frame C:
***nqikknik*eyyyh*l*fysl*iqpll*rlilvvqpnhiklsitklg*knklinsii
lklany*vsrevqtdqnfklnlmih*efkfqllsmlkptgqivlplvrfkikqdvvlvgl
lvlqnqllivyvftimkmfn*vlwiwllvmklitvvkvvmpslhgig*eskvlyqknvfh
iqfqlvhqlnnhv*ilstlhhvlksvnqipl*filktnikwlkfilstlmkqsckklllm
vqsklvslslkislltnlvftstqlvki*vvtvlnslvsvp*mvxitmplttngqlqgvi
melsnqtw*lrylydv
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFK428 (VFK428Q) /CSM/VF/VFK4-B/VFK428Q.Seq.d/                       1822   0.0
VFI438 (VFI438Q) /CSM/VF/VFI4-B/VFI438Q.Seq.d/                       1786   0.0
VFI213 (VFI213Q) /CSM/VF/VFI2-A/VFI213Q.Seq.d/                       1786   0.0
VFD555 (VFD555Q) /CSM/VF/VFD5-C/VFD555Q.Seq.d/                       1786   0.0
VSD136 (VSD136Q) /CSM/VS/VSD1-B/VSD136Q.Seq.d/                       1784   0.0
VFE574 (VFE574Q) /CSM/VF/VFE5-D/VFE574Q.Seq.d/                       1784   0.0
SLH613 (SLH613Q) /CSM/SL/SLH6-A/SLH613Q.Seq.d/                       1784   0.0
VFI120 (VFI120Q) /CSM/VF/VFI1-A/VFI120Q.Seq.d/                       1764   0.0
VFH406 (VFH406Q) /CSM/VF/VFH4-A/VFH406Q.Seq.d/                       1760   0.0
VFN645 (VFN645Q) /CSM/VF/VFN6-B/VFN645Q.Seq.d/                       1719   0.0
own update 2001.12. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BG132206|BG132206.1 EST465098 tomato crown gall Lycopersicon esculentum cDNA clone cTOE6F18 5' sequence, mRNA sequence.    54   1e-04  2
BE459071|BE459071.1 EST414363 tomato developing/immature green fruit Lycopersicon esculentum cDNA clone cLEM5M8, mRNA sequence.    54   0.004  1
BM411000|BM411000.1 EST585327 tomato breaker fruit Lycopersicon esculentum cDNA clone cLEG55G20 5' end, mRNA sequence.    54   0.004  1
BG889159|BG889159.1 EST515010 cSTD Solanum tuberosum cDNA clone cSTD13M7 5' sequence, mRNA sequence.    54   0.004  1
BF187223|BF187223.1 EST443510 potato stolon, Cornell University Solanum tuberosum cDNA clone cSTA39F1 5' sequence, mRNA sequence.    54   0.004  1
AI772499|AI772499.1 EST253599 tomato resistant, Cornell Lycopersicon esculentum cDNA clone cLER2J6, mRNA sequence.    54   0.004  1
BM535885|BM535885.1 EST588907 tomato breaker fruit Lycopersicon esculentum cDNA clone cLEG70A23 5' end, mRNA sequence.    54   0.004  1
BE353311|BE353311.1 EST400443 L. hirsutum trichome, Cornell University Lycopersicon hirsutum cDNA clone cLHT35N21, mRNA sequence.    54   0.004  1
BI421286|BI421286.1 EST531952 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC66O18 5' end, mRNA sequence.    54   0.004  1
CB610617|CB610617.1 ALBEDO0003_IIaF_D04 Mature Albedo Citrus sinensis cDNA clone ALBEDO0003_IIaF_D04 5', mRNA sequence.    54   0.004  1
dna update 2003.10.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54QD9) RecName: Full=Cathepsin B;          EC=3.4.22.1; AltNam...   587   e-167
(P00787) RecName: Full=Cathepsin B;          EC=3.4.22.1; AltNam...   214   3e-54
(P07688) RecName: Full=Cathepsin B;          EC=3.4.22.1; AltNam...   214   3e-54
BC072490_1(BC072490|pid:none) Rattus norvegicus cathepsin B, mRN...   213   6e-54
M11305_1(M11305|pid:none) Rat cathepsin B mRNA, 3' end.               213   8e-54
(A1E295) RecName: Full=Cathepsin B;          EC=3.4.22.1; Contai...   213   8e-54
EU532428_1(EU532428|pid:none) Sus scrofa cathepsin B (CTSB) gene...   211   2e-53
AC149038_4(AC149038|pid:none) Medicago truncatula chromosome 7 c...   211   4e-53
BC115254_1(BC115254|pid:none) Danio rerio capthepsin B, b, mRNA ...   209   9e-53
AC149038_3(AC149038|pid:none) Medicago truncatula chromosome 7 c...   209   1e-52
protein update 2009. 7.11
PSORT 

 psg: 0.81  gvh: 0.68  alm: 0.19  top: 0.40  tms: 0.07  mit: 0.43  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.36  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: Golgi
	 20.0 %: endoplasmic reticulum
	 16.0 %: mitochondrial
	 12.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VFK428 is cyt
5' end seq. ID VFK428F
5' end seq. 
>VFK428F.Seq
AGTAATAATAAAATCAAATAAAAAAAAATATAAAATGAGAGTATTATTATCATTAGTTGT
AATTTTATTCATTATAAATTCAGCCTTTGCTGTAAAGATTAATATTGGTCGTCCAACCAA
ATCACATAAAACTATCCATCACGAAACTTGGGTAGAAGAACAAACTGATCAATTCGATAA
TATTAAAGTTGGCCAATTATTAGGTTTCAAGAGAAGTCCAAACAGACCAAAACTTCAAAT
TAAATCTTATGATCCATTAGGAGTTCAAATTCCAACTTCTTTCAATGCTCAAACCAACTG
GCCAAATTGTACTACCATTAGTCAGATTCAAAATCAAGCAAGATGTGGTTCTTGTTGGGC
TTTTGGTGCTACAGAATCAGCTACTGATCGTTTATGTATTCACAATAATGAAAATGTTCA
ATTAAGTTTTATGGATATGGTTACTTGTGATGAAACTGATAACGGTTGTGAAGGTGGTGA
TGCCTTCTCTGCATGGAATTGGTTAAGAAAGCAAGGTGCTGTATCAGAAGAATGTCTTCC
ATATACAATTCCAACTTGTCCACCAGCTCAACAACCATGTTTAAATTTTGTCAACACTCC
ATCATGTACTAAAGAGTGTCAATCAAATTCCTCTTTANNNNNNNNNN
Length of 5' end seq. 647
3' end seq. ID VFK428Z
3' end seq. 
>VFK428Z.Seq
NNNNNNNNNNTCCATTAGGAGTTCAAATTCCAACTTCTTTCAATGCTCAAACCAACTGGC
CAAATTGTACTACCATTAGTCAGATTCAAAATCAAGCAAGATGTGGTTCTTGTTGGGCTT
TTGGTGCTACAGAATCAGCTACTGATCGTTTATGTATTCACAATAATGAAAATGTTCAAT
TAAGTTTTATGGATATGGTTACTTGTGATGAAACTGATAACGGTTGTGAAGGTGGTGATG
CCTTCTCTGCATGGAATTGGTTAAGAAAGCAAGGTGCTGTATCAGAAGAATGTCTTCCAT
ATACAATTCCAACTTGTCCACCAGCTCAACAACCATGTTTAAATTTTGTCAACACTCCAT
CATGTACTAAAGAGTGTCAATCAAATTCCTCTTTAATTTATTCTCAAGACAAACATAAAA
TGGCTAAAATTTATTCTTTCGACTCTGATGAAGCAATCATGCAAGAAATTGTTACTAATG
GTCCAGTCGAAGCTTGTTTCACTGTCTTTGAAGATTTCCTTGCTTACAAATCTGGTGTTT
ACGTCCACACAACTGGTAAAGATTTAGGTGGTCACTGTGTTAAACTCGTTGGTTTCGGTA
CCTTAAATGGTGTTNATTACTATGCCGCTAACAACCAATGGACAACTTCAAGGGGTNATA
ATGGAACTTTCTAATCAAACGTGGTGATTGCGGTATCTCTATGACGTTGT
Length of 3' end seq. 710
Connected seq. ID VFK428P
Connected seq. 
>VFK428P.Seq
AGTAATAATAAAATCAAATAAAAAAAAATATAAAATGAGAGTATTATTATCATTAGTTGT
AATTTTATTCATTATAAATTCAGCCTTTGCTGTAAAGATTAATATTGGTCGTCCAACCAA
ATCACATAAAACTATCCATCACGAAACTTGGGTAGAAGAACAAACTGATCAATTCGATAA
TATTAAAGTTGGCCAATTATTAGGTTTCAAGAGAAGTCCAAACAGACCAAAACTTCAAAT
TAAATCTTATGATCCATTAGGAGTTCAAATTCCAACTTCTTTCAATGCTCAAACCAACTG
GCCAAATTGTACTACCATTAGTCAGATTCAAAATCAAGCAAGATGTGGTTCTTGTTGGGC
TTTTGGTGCTACAGAATCAGCTACTGATCGTTTATGTATTCACAATAATGAAAATGTTCA
ATTAAGTTTTATGGATATGGTTACTTGTGATGAAACTGATAACGGTTGTGAAGGTGGTGA
TGCCTTCTCTGCATGGAATTGGTTAAGAAAGCAAGGTGCTGTATCAGAAGAATGTCTTCC
ATATACAATTCCAACTTGTCCACCAGCTCAACAACCATGTTTAAATTTTGTCAACACTCC
ATCATGTACTAAAGAGTGTCAATCAAATTCCTCTTTA----------TCCATTAGGAGTT
CAAATTCCAACTTCTTTCAATGCTCAAACCAACTGGCCAAATTGTACTACCATTAGTCAG
ATTCAAAATCAAGCAAGATGTGGTTCTTGTTGGGCTTTTGGTGCTACAGAATCAGCTACT
GATCGTTTATGTATTCACAATAATGAAAATGTTCAATTAAGTTTTATGGATATGGTTACT
TGTGATGAAACTGATAACGGTTGTGAAGGTGGTGATGCCTTCTCTGCATGGAATTGGTTA
AGAAAGCAAGGTGCTGTATCAGAAGAATGTCTTCCATATACAATTCCAACTTGTCCACCA
GCTCAACAACCATGTTTAAATTTTGTCAACACTCCATCATGTACTAAAGAGTGTCAATCA
AATTCCTCTTTAATTTATTCTCAAGACAAACATAAAATGGCTAAAATTTATTCTTTCGAC
TCTGATGAAGCAATCATGCAAGAAATTGTTACTAATGGTCCAGTCGAAGCTTGTTTCACT
GTCTTTGAAGATTTCCTTGCTTACAAATCTGGTGTTTACGTCCACACAACTGGTAAAGAT
TTAGGTGGTCACTGTGTTAAACTCGTTGGTTTCGGTACCTTAAATGGTGTTNATTACTAT
GCCGCTAACAACCAATGGACAACTTCAAGGGGTNATAATGGAACTTTCTAATCAAACGTG
GTGATTGCGGTATCTCTATGACGTTGT
Length of connected seq. 1337
Full length Seq ID VFK428E
Full length Seq. 
>VFK428E.Seq
AGTAATAATAAAATCAAATAAAAAAAAATATAAAATGAGAGTATTATTATCATTAGTTGT
AATTTTATTCATTATAAATTCAGCCTTTGCTGTAAAGATTAATATTGGTCGTCCAACCAA
ATCACATAAAACTATCCATCACGAAACTTGGGTAGAAGAACAAACTGATCAATTCGATAA
TATTAAAGTTGGCCAATTATTAGGTTTCAAGAGAAGTCCAAACAGACCAAAACTTCAAAT
TAAATCTTATGATCCATTAGGAGTTCAAATTCCAACTTCTTTCAATGCTCAAACCAACTG
GCCAAATTGTACTACCATTAGTCAGATTCAAAATCAAGCAAGATGTGGTTCTTGTTGGGC
TTTTGGTGCTACAGAATCAGCTACTGATCGTTTATGTATTCACAATAATGAAAATGTTCA
ATTAAGTTTTATGGATATGGTTACTTGTGATGAAACTGATAACGGTTGTGAAGGTGGTGA
TGCCTTCTCTGCATGGAATTGGTTAAGAAAGCAAGGTGCTGTATCAGAAGAATGTCTTCC
ATATACAATTCCAACTTGTCCACCAGCTCAACAACCATGTTTAAATTTTGTCAACACTCC
ATCATGTACTAAAGAGTGTCAATCAAATTCCTCTTTAATTTATTCTCAAGACAAACATAA
AATGGCTAAAATTTATTCTTTCGACTCTGATGAAGCAATCATGCAAGAAATTGTTACTAA
TGGTCCAGTCGAAGCTTGTTTCACTGTCTTTGAAGATTTCCTTGCTTACAAATCTGGTGT
TTACGTCCACACAACTGGTAAAGATTTAGGTGGTCACTGTGTTAAACTCGTTGGTTTCGG
TACCTTAAATGGTGTTNATTACTATGCCGCTAACAACCAATGGACAACTTCAAGGGGTNA
TAATGGAACTTTCTAATCAAACGTGGTGATTGCGGTATCTCTATGACGTTGT
Length of full length seq. 952