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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM815
Library AF
(Link to library)
Clone ID AFM815
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM815P
(Link to Original site)
Representative DNA sequence 
>AFM815 (AFM815Q) /CSM/AF/AFM8-A/AFM815Q.Seq.d/
AAACACACTAACACATAAATAATATTTATCTATCTATCCACACACCCAAAGTATTCATAT
TAATGGCAGAAATAACAATATCAATTGAAAATGAAAATTTCATTCAAGTAAATTTACAAC
CAGATCAAACAGTTGAAGATCTAAAACGTAGAGTAGAATTTGAAACCACAATTTTAGTGA
ACAATCAAGTTTTAACACTTGATGGAAAAGTATTAGATAATGAAAAGAAATTATCAGACT
ATTCAATTAAAGGTGGTGATTTCTTATTGATAACAAAAAATGTTTTAAGAGCACCACAAC
AAAGATCACAACAACCCCAACAACCACAACAACCACAACAACCACAACAACAACGTCAAC
CACAAAGAGATCCTCTCAATTCACCACAAGATATTTTAGACCACTTTACAAACAATCCTG
AAGACTTAACTCAAGTTATAAATTCAAATCCAGCATTAGCAAATGCAATCTTAAGTAAAG
ATATGAAATTCCTTACTCACTTTGTAGAACAAATCAAAGAACAACGTCGTATTCAAGAAT
TGGCATTAAAAGATCCATATGGTGAAGAGTATCAAAAATTAGCTTATCAACATATTCAAC
AACAAAATATTGAAAAAAATATGCAACATGCTATGGAACXXXXXXXXXXGCTTTTGTAGA
TNCAGGTGCCCANCAATNTATTATGTNTGAAAAATGTGNNGAAAGATGGGNAATTTCAAG
AATTATTGATANTAGATTTCATGGTATTGNTAAAGGTGTGGGTACATCAAAAATTATAGG
TAGAGTTCATAGTACAGACTTAAAACTTGGCAACTCTTTATTNTCTGTATCATTATCTAT
CCTTCAAAATCAAGATGTTGACTTCATCCTTGGTTTGGATATGTTAAAGCGTCATCAAGT
CATTTTGGATTTAAATAGAGGTGTATTACAAATTGCAAATGAAAAGATTGAATTCCTTCA
TGAAAAAGATTTAAAGGAGATTTTAAATAAAGAACAAAATGATTTAGAAGAAGATACTAA
AAAAGCANCTGCTAATAGTCTTTCACCTTCAACAACTTCAACCACTACCACTACCACTAC
CACTACTTCACCCCAAACTCCAACTAATGCCACTTCTCCTGTAAATAATAATAATAATAA
TAATAACAACAACAACAACACTAGACCTCCAATTAATTCACCACCAGTTGCAAAGACACC
GCAACCAACACCACAACCAACTACAAATCAATATGCTTCTGAATCCAATATCCAAACCCT
TATTGGACTTGGTTGTTCAAGAGATAAGGCAATTAGACTTTTAACTCAAGACTAAAGGCA
ATGT
sequence update 2009. 4. 3
Translated Amino Acid sequence 
th*HINNIYLSIHTPKVFILMAEITISIENENFIQVNLQPDQTVEDLKRRVEFETTILVN
NQVLTLDGKVLDNEKKLSDYSIKGGDFLLITKNVLRAPQQRSQQPQQPQQPQQPQQQRQP
QRDPLNSPQDILDHFTNNPEDLTQVINSNPALANAILSKDMKFLTHFVEQIKEQRRIQEL
ALKDPYGEEYQKLAYQHIQQQNIEKNMQHAME---

---AFVDXGAXQXIMXEKCXERWXISRIIDXRFHGIXKGVGTSKIIGRVHSTDLKLGNSL
XSVSLSILQNQDVDFILGLDMLKRHQVILDLNRGVLQIANEKIEFLHEKDLKEILNKEQN
DLEEDTKKAXANSLSPSTTSTTTTTTTTTSPQTPTNATSPVNNNNNNNNNNNNTRPPINS
PPVAKTPQPTPQPTTNQYASESNIQTLIGLGCSRDKAIRLLTQD*rqc
Translated Amino Acid sequence (All Frames) 
Frame A:
khtnt*iifiylsthpkysy*wqk*qyqlkmkisfk*iynqikqlki*nve*nlkpqf**
tikf*hlmeky*imkrnyqtiqlkvvisy**qkmf*ehhnkdhnnpnnhnnhnnhnnnvn
hkeilsihhkif*ttlqtilkt*lkl*iqiqh*qmqs*vki*nslltl*nksknnvvfkn
wh*kihmvksikn*linifnnkilkkicnmlwn---

---AFVDXGAXQXIMXEKCXERWXISRIIDXRFHGIXKGVGTSKIIGRVHSTDLKLGNSL
XSVSLSILQNQDVDFILGLDMLKRHQVILDLNRGVLQIANEKIEFLHEKDLKEILNKEQN
DLEEDTKKAXANSLSPSTTSTTTTTTTTTSPQTPTNATSPVNNNNNNNNNNNNTRPPINS
PPVAKTPQPTPQPTTNQYASESNIQTLIGLGCSRDKAIRLLTQD*rqc

Frame B:
ntlthk*ylsiyphtqsihingrnnnin*k*kfhsskfttrsns*rskt*sri*nhnfse
qssfnt*wksir**keiirlfn*rw*flidnkkcfkstttkitttpttttttttttttst
tkrssqfttryfrplykqs*rlnssykfkssiskcnlk*ryeipyslcrtnqrttsysri
gikrsiw*rvskislstysttky*kkyatcyg---

---ll*xqvpxnxlcxknvxkdgxfqellixdfmvlxkvwvhqkl*vefivqt*nlatly
xlyhylsfkikmltsslvwic*sviksfwi*ievyyklqmkrlnsfmkki*rrf*iknkm
i*kkilkkxllivfhlqqlqplplplpllhpklqlmplll*iiiiiiitttttldlqlih
hqlqrhrnqhhnqlqinmllnpiskplldlvvqeirqldf*lktkgn

Frame C:
th*HINNIYLSIHTPKVFILMAEITISIENENFIQVNLQPDQTVEDLKRRVEFETTILVN
NQVLTLDGKVLDNEKKLSDYSIKGGDFLLITKNVLRAPQQRSQQPQQPQQPQQPQQQRQP
QRDPLNSPQDILDHFTNNPEDLTQVINSNPALANAILSKDMKFLTHFVEQIKEQRRIQEL
ALKDPYGEEYQKLAYQHIQQQNIEKNMQHAME---

---fcrxrcpxxyyv*kmxxkmgnfkny*x*iswyx*rcgyiknyr*ss*yrlktwqlfi
xciiiypsksrc*lhpwfgyvkassshfgfk*rcitnck*kd*ips*krfkgdfk*rtk*
frrry*ksxc**sftfnnfnhyhyhyhyftpnsn*chfsck*******qqqqh*tsn*ft
tsckdtatntttnyksicf*iqypnpywtwlfkr*gn*tfnsrlkam
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM815 (AFM815Q) /CSM/AF/AFM8-A/AFM815Q.Seq.d/                       1332   0.0
CFI315 (CFI315Q) /CSM/CF/CFI3-A/CFI315Q.Seq.d/                        862   0.0
AFJ439 (AFJ439Q) /CSM/AF/AFJ4-B/AFJ439Q.Seq.d/                        854   0.0
AFJ488 (AFJ488Q) /CSM/AF/AFJ4-D/AFJ488Q.Seq.d/                        831   0.0
VFE196 (VFE196Q) /CSM/VF/VFE1-D/VFE196Q.Seq.d/                        825   0.0
SFL211 (SFL211Q) /CSM/SF/SFL2-A/SFL211Q.Seq.d/                        823   0.0
CHA120 (CHA120Q) /CSM/CH/CHA1-A/CHA120Q.Seq.d/                        807   0.0
CHD380 (CHD380Q) /CSM/CH/CHD3-D/CHD380Q.Seq.d/                        805   0.0
CHA270 (CHA270Q) /CSM/CH/CHA2-C/CHA270Q.Seq.d/                        805   0.0
AFE196 (AFE196Q) /CSM/AF/AFE1-D/AFE196Q.Seq.d/                        799   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ329759) Dictyostelium discoideum cDNA clone:dda26n04, 5' ...   577   0.0    3
(BJ361763) Dictyostelium discoideum cDNA clone:ddc18l13, 5' ...   577   0.0    3
(BJ328686) Dictyostelium discoideum cDNA clone:dda29h02, 5' ...   577   0.0    3
(BJ362274) Dictyostelium discoideum cDNA clone:ddc21i01, 5' ...   577   0.0    3
(BJ327166) Dictyostelium discoideum cDNA clone:dda19n10, 5' ...   577   0.0    3
(BJ325145) Dictyostelium discoideum cDNA clone:dda9o23, 5' e...   577   0.0    3
(BJ329218) Dictyostelium discoideum cDNA clone:dda24p04, 5' ...   577   0.0    3
(BJ324588) Dictyostelium discoideum cDNA clone:dda7d03, 5' e...   577   0.0    3
(BJ327290) Dictyostelium discoideum cDNA clone:dda19l22, 5' ...   577   0.0    3
(BJ386823) Dictyostelium discoideum cDNA clone:dds10h22, 5' ...   577   0.0    3
dna update 2009. 4.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54JB0) RecName: Full=Protein DDI1 homolog;                          321   e-167
AK067974_1(AK067974|pid:none) Oryza sativa Japonica Group cDNA c...    98   3e-35
DQ311262_1(DQ311262|pid:none) Bombyx mori DNA-damage inducible p...   106   1e-34
(A0JPP7) RecName: Full=Protein DDI1 homolog 1;  &BC127531_1(BC12...    99   8e-31
(Q5TDH0) RecName: Full=Protein DDI1 homolog 2;  &AK293006_1(AK29...   102   3e-30
(Q7S906) RecName: Full=DNA damage-inducible protein 1;                102   7e-30
(Q7ZYA7) RecName: Full=Protein DDI1 homolog 2;  &BC043869_1(BC04...   100   1e-29
(Q6TH22) RecName: Full=Protein DDI1 homolog 2;  &AY398334_1(AY39...   103   2e-29
(Q9DAF3) RecName: Full=Protein DDI1 homolog 1;  &AK005882_1(AK00...    98   2e-29
AM910995_387(AM910995|pid:none) Plasmodium knowlesi strain H chr...   115   2e-29
protein update 2009. 4. 6
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM815 is

5' end seq. ID AFM815F
5' end seq. 
>AFM815F.Seq
AAACACACTAACACATAAATAATATTTATCTATCTATCCACACACCCAAAGTATTCATAT
TAATGGCAGAAATAACAATATCAATTGAAAATGAAAATTTCATTCAAGTAAATTTACAAC
CAGATCAAACAGTTGAAGATCTAAAACGTAGAGTAGAATTTGAAACCACAATTTTAGTGA
ACAATCAAGTTTTAACACTTGATGGAAAAGTATTAGATAATGAAAAGAAATTATCAGACT
ATTCAATTAAAGGTGGTGATTTCTTATTGATAACAAAAAATGTTTTAAGAGCACCACAAC
AAAGATCACAACAACCCCAACAACCACAACAACCACAACAACCACAACAACAACGTCAAC
CACAAAGAGATCCTCTCAATTCACCACAAGATATTTTAGACCACTTTACAAACAATCCTG
AAGACTTAACTCAAGTTATAAATTCAAATCCAGCATTAGCAAATGCAATCTTAAGTAAAG
ATATGAAATTCCTTACTCACTTTGTAGAACAAATCAAAGAACAACGTCGTATTCAAGAAT
TGGCATTAAAAGATCCATATGGTGAAGAGTATCAAAAATTAGCTTATCAACATATTCAAC
AACAAAATATTGAAAAAAATATGCAACATGCTATGGAACXXXXXXXXXX
Length of 5' end seq. 649
3' end seq. ID AFM815Z
3' end seq. 
>AFM815Z.Seq
XXXXXXXXXXGCTTTTGTAGATNCAGGTGCCCANCAATNTATTATGTNTGAAAAATGTGN
NGAAAGATGGGNAATTTCAAGAATTATTGATANTAGATTTCATGGTATTGNTAAAGGTGT
GGGTACATCAAAAATTATAGGTAGAGTTCATAGTACAGACTTAAAACTTGGCAACTCTTT
ATTNTCTGTATCATTATCTATCCTTCAAAATCAAGATGTTGACTTCATCCTTGGTTTGGA
TATGTTAAAGCGTCATCAAGTCATTTTGGATTTAAATAGAGGTGTATTACAAATTGCAAA
TGAAAAGATTGAATTCCTTCATGAAAAAGATTTAAAGGAGATTTTAAATAAAGAACAAAA
TGATTTAGAAGAAGATACTAAAAAAGCANCTGCTAATAGTCTTTCACCTTCAACAACTTC
AACCACTACCACTACCACTACCACTACTTCACCCCAAACTCCAACTAATGCCACTTCTCC
TGTAAATAATAATAATAATAATAATAACAACAACAACAACACTAGACCTCCAATTAATTC
ACCACCAGTTGCAAAGACACCGCAACCAACACCACAACCAACTACAAATCAATATGCTTC
TGAATCCAATATCCAAACCCTTATTGGACTTGGTTGTTCAAGAGATAAGGCAATTAGACT
TTTAACTCAAGACTAAAGGCAATGT
Length of 3' end seq. 685
Connected seq. ID AFM815P
Connected seq. 
>AFM815P.Seq
AAACACACTAACACATAAATAATATTTATCTATCTATCCACACACCCAAAGTATTCATAT
TAATGGCAGAAATAACAATATCAATTGAAAATGAAAATTTCATTCAAGTAAATTTACAAC
CAGATCAAACAGTTGAAGATCTAAAACGTAGAGTAGAATTTGAAACCACAATTTTAGTGA
ACAATCAAGTTTTAACACTTGATGGAAAAGTATTAGATAATGAAAAGAAATTATCAGACT
ATTCAATTAAAGGTGGTGATTTCTTATTGATAACAAAAAATGTTTTAAGAGCACCACAAC
AAAGATCACAACAACCCCAACAACCACAACAACCACAACAACCACAACAACAACGTCAAC
CACAAAGAGATCCTCTCAATTCACCACAAGATATTTTAGACCACTTTACAAACAATCCTG
AAGACTTAACTCAAGTTATAAATTCAAATCCAGCATTAGCAAATGCAATCTTAAGTAAAG
ATATGAAATTCCTTACTCACTTTGTAGAACAAATCAAAGAACAACGTCGTATTCAAGAAT
TGGCATTAAAAGATCCATATGGTGAAGAGTATCAAAAATTAGCTTATCAACATATTCAAC
AACAAAATATTGAAAAAAATATGCAACATGCTATGGAAC----------GCTTTTGTAGA
TNCAGGTGCCCANCAATNTATTATGTNTGAAAAATGTGNNGAAAGATGGGNAATTTCAAG
AATTATTGATANTAGATTTCATGGTATTGNTAAAGGTGTGGGTACATCAAAAATTATAGG
TAGAGTTCATAGTACAGACTTAAAACTTGGCAACTCTTTATTNTCTGTATCATTATCTAT
CCTTCAAAATCAAGATGTTGACTTCATCCTTGGTTTGGATATGTTAAAGCGTCATCAAGT
CATTTTGGATTTAAATAGAGGTGTATTACAAATTGCAAATGAAAAGATTGAATTCCTTCA
TGAAAAAGATTTAAAGGAGATTTTAAATAAAGAACAAAATGATTTAGAAGAAGATACTAA
AAAAGCANCTGCTAATAGTCTTTCACCTTCAACAACTTCAACCACTACCACTACCACTAC
CACTACTTCACCCCAAACTCCAACTAATGCCACTTCTCCTGTAAATAATAATAATAATAA
TAATAACAACAACAACAACACTAGACCTCCAATTAATTCACCACCAGTTGCAAAGACACC
GCAACCAACACCACAACCAACTACAAATCAATATGCTTCTGAATCCAATATCCAAACCCT
TATTGGACTTGGTTGTTCAAGAGATAAGGCAATTAGACTTTTAACTCAAGACTAAAGGCA
ATGT
Length of connected seq. 1314
Full length Seq ID -
Full length Seq. -
Length of full length seq. -