>VFK342 (VFK342Q) /CSM/VF/VFK3-B/VFK342Q.Seq.d/
AAAAAGCACACCATAACATTGTATTTATATATAAATATTATCACACAAATTATTAAATTA
TTACTAGTATATATAATTATTTAGAATAATAAAATAATAATAATAAAAATAATAATAACC
GAATTTTAAAATTAATAAAAACTAAACAACTATAATATATAAATAGATTTAAAAAAAAAA
TATATAAAAAATGTCATCAGATATTTCAGAAAAAATTCAACAAGCAAGAAGAGATGCTGA
ATCAATGAAAGAACAAATTAGAGCAAACAGAGATGTAATGAATGATACTACTCTCAAAAC
TTTTACTCGTGATTTACCAGGTCTTCCAAAGATGGAAGGTAAAATTAAAGTTAGAAGAAA
CCTTAAGGGTCATCTTGCTAAAATCTATGCTATGCATTGGGCTGAAGATAATGTTCATTT
AGTTTCTGCCTCTCAAGATGGTAAATTATTAGTTTGGGATGGTTTAACTACCAACAAAGT
CCACGCTATTCCATTAAGATCATCATGGGTTATGACATGTGCTTATTCACCAACTGCTAA
CTTTGTXXXXXXXXXXTGTTCCATTTATAATTTACGTAGTAGAGAACAACCAATTAGAGT
ATGCCGTGAATTAAACTCTCACACTGGTTATCTCTCATGTTGTAGATTCCTCAATGATAG
ACAAATTGTTACTTCAAGTGGTGATATGACATGTATTTTATGGGATGTTGAAAATGGTAC
AAAGATCACAGAATTCTCTGACCACAACGGGTGATGTCATGAGTGTCAGTGTTTCACCAG
ATAAAAACTATTTCATCAGTGGTGCTTGTGATGCTACCGCCAAATTATGGGATTTACGTA
GTGGTAAATGTGTTCAAACTTTCACTGGTCACGAAGCTGATATTAATGCAGTTCAATATT
TCCCAAATGGTTTATCATTTGGTACTGGTTCTGATGATGCTTCATGTAGATTATTTGATA
TTCGTGCCGATCGTGAATTAATGCAATACACCCACGACAACATTTTATGTGGTATTACAT
CCGTTGGTTTTTCATTTTCAGGTCGTTTCTTATTTGCTGGTTACGATGATTTCACTTGCA
ACGTTTGGGATACATTAAAGGGTGAACGTGTTCTCTCTTTAACNGGTCACGGTAATCGTG
TATCTTGTCTTGGTGTACCAACCGATGGTATGGCTTTATGTACAGGTAGTGGGATTCTCT
CCTCAAGATTGGGCTAAATTTTT

kstp*hciyi*ilshkllnyy*yi*lfriik****k***pnfkinkn*ttiiyk*i*kkn
i*KMSSDISEKIQQARRDAESMKEQIRANRDVMNDTTLKTFTRDLPGLPKMEGKIKVRRN
LKGHLAKIYAMHWAEDNVHLVSASQDGKLLVWDGLTTNKVHAIPLRSSWVMTCAYSPTAN
F---

---CSIYNLRSREQPIRVCRELNSHTGYLSCCRFLNDRQIVTSSGDMTCILWDVENGTKI
TEFSDHNG*checqcftr*klfhqwcl*cyrqimgft*w*mcsnfhwsrs*y*cssifpk
wfiiwywf**cfm*ii*yscrs*inaihprqhfmwyyirwffifrsflicwlr*fhlqrl
gyikg*tcslfnxsr*scilswctnrwygfmyr*wdsllkiglnf

Frame A:
kkhtitlylyiniitqiiklllvyiii*nnkiiiikiiitef*n**klnnyni*idlkkk
yiknviryfrknstskkrc*inertn*skqrcne*yysqnfys*ftrsskdgr*n*s*kk
p*gssc*nlcyalg*r*csfsfclsrw*iislgwfnyqqsprysikiimgydmclftnc*
lc---

---CSIYNLRSREQPIRVCRELNSHTGYLSCCRFLNDRQIVTSSGDMTCILWDVENGTKI
TEFSDHNG*checqcftr*klfhqwcl*cyrqimgft*w*mcsnfhwsrs*y*cssifpk
wfiiwywf**cfm*ii*yscrs*inaihprqhfmwyyirwffifrsflicwlr*fhlqrl
gyikg*tcslfnxsr*scilswctnrwygfmyr*wdsllkiglnf

Frame B:
kstp*hciyi*ilshkllnyy*yi*lfriik****k***pnfkinkn*ttiiyk*i*kkn
i*KMSSDISEKIQQARRDAESMKEQIRANRDVMNDTTLKTFTRDLPGLPKMEGKIKVRRN
LKGHLAKIYAMHWAEDNVHLVSASQDGKLLVWDGLTTNKVHAIPLRSSWVMTCAYSPTAN
F---

---vpfiiyvvennqleyavn*tltlvishvvdssmidklllqvvi*hvfygmlkmvqrs
qnsltttgdvmsvsvspdknyfisgacdataklwdlrsgkcvqtftgheadinavqyfpn
glsfgtgsddascrlfdiradrelmqythdnilcgitsvgfsfsgrflfagyddftcnvw
dtlkgervlsltghgnrvsclgvptdgmalctgsgilssrlg*if

Frame C:
kahhnivfiykyyhtny*iitsiynyle**nnnnknnnnrilkliktkql*yinrfkkki
ykkchqifqkkfnkqeemlnq*knkleqtem**millsklllviyqvfqrwkvklkleet
lrvillksmlciglkimfi*flplkmvny*fgmv*lptkstlfh*dhhgl*hvlihqllt
l---

---fhl*ft**rttn*smp*iklshwlslml*ipq**tncyfkw*ydmyfmgc*kwykdh
ril*pqrvms*vsvfhqiktissvvlvmlppnygiyvvvnvfklslvtklilmqfnisqm
vyhlvlvlmmlhvdylifvpivn*cntptttfyvvlhplvfhfqvvsyllvtmislatfg
ih*rvnvfsl*xvtvivylvlvyqpmvwlyvqvvgfspqdwakf

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFK342 (VFK342Q) /CSM/VF/VFK3-B/VFK342Q.Seq.d/                       2002   0.0
AFJ370 (AFJ370Q) /CSM/AF/AFJ3-C/AFJ370Q.Seq.d/                       1063   0.0
AFK519 (AFK519Q) /CSM/AF/AFK5-A/AFK519Q.Seq.d/                       1027   0.0
AFO321 (AFO321Q) /CSM/AF/AFO3-A/AFO321Q.Seq.d/                        811   0.0
SSD702 (SSD702Q) /CSM/SS/SSD7-A/SSD702Q.Seq.d/                        729   0.0
SLA519 (SLA519Q) /CSM/SL/SLA5-A/SLA519Q.Seq.d/                        726   0.0
SFB734 (SFB734Q) /CSM/SF/SFB7-B/SFB734Q.Seq.d/                        726   0.0
AFO746 (AFO746Q) /CSM/AF/AFO7-B/AFO746Q.Seq.d/                        726   0.0
AFM380 (AFM380Q) /CSM/AF/AFM3-D/AFM380Q.Seq.d/                        718   0.0
SLD136 (SLD136Q) /CSM/SL/SLD1-B/SLD136Q.Seq.d/                        660   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X73641|X73641.1 Dictyostelium discoideum gene for G-protein beta-subunit.   868   0.0    4
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.   579   0.0    15
Z23105|Z23105.1 L.stagnalis mRNA for G protein beta subunit.       52   7e-12  2
BM475096|BM475096.1 AGENCOURT_6477641 NIH_MGC_88 Homo sapiens cDNA clone IMAGE:5559167 5', mRNA sequence.    50   1e-10  5
AF300648|AF300648.1 Homo sapiens guanine nucleotide binding protein beta subunit 4 (GNB4) mRNA, complete cds.    50   1e-10  5
BC000873|BC000873.1 Homo sapiens, guanine nucleotide binding protein beta subunit 4, clone MGC:5262 IMAGE:3460570, mRNA, complete cds.    50   3e-10  5
CF600903|CF600903.1 tac43g06.y1 Hydra EST -IV Hydra magnipapillata cDNA 5' similar to SW:GBB1_XENLA P79959 GUANINE NUCLEOTIDE-BINDING PROTEIN G(I)/G(S)/G(T) BETA SUBUNIT 1 ;, mRNA sequence.    56   4e-10  3
BD156834|BD156834.1 Primer for synthesizing full-length cDNA and use thereof.    50   4e-09  5
AK001890|AK001890.1 Homo sapiens cDNA FLJ11028 fis, clone PLACE1004128, highly similar to GUANINE NUCLEOTIDE-BINDING PROTEIN BETA SUBUNIT 4.    50   4e-09  5
CF601902|CF601902.1 tac43g06.x1 Hydra EST -IV Hydra magnipapillata cDNA 3' similar to SW:GBB1_XENLA P79959 GUANINE NUCLEOTIDE-BINDING PROTEIN G(I)/G(S)/G(T) BETA SUBUNIT 1 ;, mRNA sequence.    56   3e-08  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36408) RecName: Full=Guanine nucleotide-binding protein subuni...   373   0.0
AF536325_1(AF536325|pid:none) Ustilago maydis G-protein beta sub...   252   e-134
AY661671_1(AY661671|pid:none) Lentinula edodes guanine nucleotid...   254   e-133
AF091120_1(AF091120|pid:none) Filobasidiella neoformans G-protei...   246   e-128
AY599846_1(AY599846|pid:none) Rhizopus stolonifer G-protein beta...   251   e-127
AF056182_1(AF056182|pid:none) Emericella nidulans G-protein beta...   246   e-126
AY550249_1(AY550249|pid:none) Paracoccidioides brasiliensis smal...   246   e-125
AY211190_1(AY211190|pid:none) Cochliobolus heterostrophus G-prot...   249   e-125
DQ863323_1(DQ863323|pid:none) Penicillium marneffei STE4-like pr...   241   e-124
AB188197_1(AB188197|pid:none) Botryotinia fuckeliana bcgb1 gene ...   241   e-124

 psg: 0.38  gvh: 0.30  alm: 0.50  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 20.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VFK342 is nuc

>VFK342F.Seq
AAAAAGCACACCATAACATTGTATTTATATATAAATATTATCACACAAATTATTAAATTA
TTACTAGTATATATAATTATTTAGAATAATAAAATAATAATAATAAAAATAATAATAACC
GAATTTTAAAATTAATAAAAACTAAACAACTATAATATATAAATAGATTTAAAAAAAAAA
TATATAAAAAATGTCATCAGATATTTCAGAAAAAATTCAACAAGCAAGAAGAGATGCTGA
ATCAATGAAAGAACAAATTAGAGCAAACAGAGATGTAATGAATGATACTACTCTCAAAAC
TTTTACTCGTGATTTACCAGGTCTTCCAAAGATGGAAGGTAAAATTAAAGTTAGAAGAAA
CCTTAAGGGTCATCTTGCTAAAATCTATGCTATGCATTGGGCTGAAGATAATGTTCATTT
AGTTTCTGCCTCTCAAGATGGTAAATTATTAGTTTGGGATGGTTTAACTACCAACAAAGT
CCACGCTATTCCATTAAGATCATCATGGGTTATGACATGTGCTTATTCACCAACTGCTAA
CTTTGTNNNNNNNNNN

>VFK342Z.Seq
NNNNNNNNNNTGTTCCATTTATAATTTACGTAGTAGAGAACAACCAATTAGAGTATGCCG
TGAATTAAACTCTCACACTGGTTATCTCTCATGTTGTAGATTCCTCAATGATAGACAAAT
TGTTACTTCAAGTGGTGATATGACATGTATTTTATGGGATGTTGAAAATGGTACAAAGAT
CACAGAATTCTCTGACCACAACGGGTGATGTCATGAGTGTCAGTGTTTCACCAGATAAAA
ACTATTTCATCAGTGGTGCTTGTGATGCTACCGCCAAATTATGGGATTTACGTAGTGGTA
AATGTGTTCAAACTTTCACTGGTCACGAAGCTGATATTAATGCAGTTCAATATTTCCCAA
ATGGTTTATCATTTGGTACTGGTTCTGATGATGCTTCATGTAGATTATTTGATATTCGTG
CCGATCGTGAATTAATGCAATACACCCACGACAACATTTTATGTGGTATTACATCCGTTG
GTTTTTCATTTTCAGGTCGTTTCTTATTTGCTGGTTACGATGATTTCACTTGCAACGTTT
GGGATACATTAAAGGGTGAACGTGTTCTCTCTTTAACNGGTCACGGTAATCGTGTATCTT
GTCTTGGTGTACCAACCGATGGTATGGCTTTATGTACAGGTAGTGGGATTCTCTCCTCAA
GATTGGGCTAAATTTTT

>VFK342P.Seq
AAAAAGCACACCATAACATTGTATTTATATATAAATATTATCACACAAATTATTAAATTA
TTACTAGTATATATAATTATTTAGAATAATAAAATAATAATAATAAAAATAATAATAACC
GAATTTTAAAATTAATAAAAACTAAACAACTATAATATATAAATAGATTTAAAAAAAAAA
TATATAAAAAATGTCATCAGATATTTCAGAAAAAATTCAACAAGCAAGAAGAGATGCTGA
ATCAATGAAAGAACAAATTAGAGCAAACAGAGATGTAATGAATGATACTACTCTCAAAAC
TTTTACTCGTGATTTACCAGGTCTTCCAAAGATGGAAGGTAAAATTAAAGTTAGAAGAAA
CCTTAAGGGTCATCTTGCTAAAATCTATGCTATGCATTGGGCTGAAGATAATGTTCATTT
AGTTTCTGCCTCTCAAGATGGTAAATTATTAGTTTGGGATGGTTTAACTACCAACAAAGT
CCACGCTATTCCATTAAGATCATCATGGGTTATGACATGTGCTTATTCACCAACTGCTAA
CTTTGT----------TGTTCCATTTATAATTTACGTAGTAGAGAACAACCAATTAGAGT
ATGCCGTGAATTAAACTCTCACACTGGTTATCTCTCATGTTGTAGATTCCTCAATGATAG
ACAAATTGTTACTTCAAGTGGTGATATGACATGTATTTTATGGGATGTTGAAAATGGTAC
AAAGATCACAGAATTCTCTGACCACAACGGGTGATGTCATGAGTGTCAGTGTTTCACCAG
ATAAAAACTATTTCATCAGTGGTGCTTGTGATGCTACCGCCAAATTATGGGATTTACGTA
GTGGTAAATGTGTTCAAACTTTCACTGGTCACGAAGCTGATATTAATGCAGTTCAATATT
TCCCAAATGGTTTATCATTTGGTACTGGTTCTGATGATGCTTCATGTAGATTATTTGATA
TTCGTGCCGATCGTGAATTAATGCAATACACCCACGACAACATTTTATGTGGTATTACAT
CCGTTGGTTTTTCATTTTCAGGTCGTTTCTTATTTGCTGGTTACGATGATTTCACTTGCA
ACGTTTGGGATACATTAAAGGGTGAACGTGTTCTCTCTTTAACNGGTCACGGTAATCGTG
TATCTTGTCTTGGTGTACCAACCGATGGTATGGCTTTATGTACAGGTAGTGGGATTCTCT
CCTCAAGATTGGGCTAAATTTTT