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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFK294
Library VF
(Link to library)
Clone ID VFK294
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11332-1
Original site URL
Representative seq. ID VFK294P
(Link to Original site)
Representative DNA sequence 
>VFK294 (VFK294Q) /CSM/VF/VFK2-D/VFK294Q.Seq.d/
GTCATTAAATAATCATCATCAACAACAACAAAATATTGTACATACTATACCAATAACAAA
TTCAATACCACCAGTAAACTCAATCACTATTTCAATATTAGCAAGCACATTAACCAAATC
GATAGTTGTAAATTTTCAAATACTAACAGCTACCAATGTTGGTAAAGTATTGAGTATCAT
TCAAATTGGTAAGAAACAACGTGAGAATACTAAACAGGGTGCTTGTTTTCACCCATTTTA
TAAATCCTCTGTTTACACATCAAGACCTGGCAAAAGATTATGGTTAGCCGATCCAATTGA
TGGTCGTGTTTTATCGACTATGAATTTCACACCATCCTCAACCACAAACGAAGATGGTAC
TACAAATCCAATACCAAATATAATATTGAAACCAAAACCATTAATATCTTCAAATTCATC
CAATGATGCAAATACTTATCTCACTGGTAAATTCCCAATGATATTCTCAAAGTTATTACC
ATTTGGTAATTACTTAATATCATATGATGAATTATCATTACTCTTAATCGATGTTAATGA
AGTTGAAGTTTTAGAATGGAAATTAGATACAAATCAAATTCATGACTTGGTTGTTTATCA
TGATTCAATCTATGCATTACATGGTCXXXXXXXXXXACATCAATTCAAAGAGTAGCATCA
AAAGAATGTATTTTCTCAGATATTCCTTTATCATCAGAACCATTAATGGTATCANACAAG
TGTTAAAGATCCAACTTTAGCTGCATCAACAACTAGAAAGGTTATAAAGAANAAGGTTAT
AAAAACAGCATTACCACAGGATCAACAATTAAATGGAACAAATAATACAGCAACTCTATC
AACTTCACCACCACAATCAATAGTATCATCATCAACAACAGCAACANCAGCAACAGCAAC
ATCAACATCAATAGGAGCACCAACAGAAGTCAAAAGAACAGTTATTGTAAAGAAAAAAAT
TATTAAAAAAGCAGCGGCATTATCAACTACAACTACAACCACACCACAAATCTCATCGCC
ACCAATTTCAAATCCTACACCATCACCACCCNAATATGTAGAAACCGTATCATCAT
sequence update 2001.11.22
Translated Amino Acid sequence 
SLNNHHQQQQNIVHTIPITNSIPPVNSITISILASTLTKSIVVNFQILTATNVGKVLSII
QIGKKQRENTKQGACFHPFYKSSVYTSRPGKRLWLADPIDGRVLSTMNFTPSSTTNEDGT
TNPIPNIILKPKPLISSNSSNDANTYLTGKFPMIFSKLLPFGNYLISYDELSLLLIDVNE
VEVLEWKLDTNQIHDLVVYHDSIYALHG---

---INSKSSIKRMYFLRYSFIIRTINGIXQVLKIQL*lhqqlerl*rxrl*kqhyhrinn
*meqiiqqlyqlhhhnq*yhhqqqqxqqqqhqhq*ehqqkskeqll*rkkllkkqrhyql
qlqphhkshrhqfqilhhhhpnm*kpyhh
Translated Amino Acid sequence (All Frames) 
Frame A:
vik*ssstttkyctyytnnkfnttsklnhyfniskhinqidsckfsntnsyqcw*sieyh
snw*ett*ey*tgclfspil*ilclhiktwqkimvsrsn*wscfidyefhtilnhkrrwy
yksntkynietktinifkfiq*ckylshw*ipndilkvitiw*llnii**iiitlnrc**
s*sfrmeiryksns*lgcls*fnlcitw---

---tsiqrvaskecifsdiplsseplmvsxkc*rsnfscinn*kgykexgyknsittgst
ikwnk*ysnsinftttinsiiinnsnxsnsnininrstnrsqknsyckekny*kssgiin
ynynhttnliatnfksytittxicrnrii

Frame B:
SLNNHHQQQQNIVHTIPITNSIPPVNSITISILASTLTKSIVVNFQILTATNVGKVLSII
QIGKKQRENTKQGACFHPFYKSSVYTSRPGKRLWLADPIDGRVLSTMNFTPSSTTNEDGT
TNPIPNIILKPKPLISSNSSNDANTYLTGKFPMIFSKLLPFGNYLISYDELSLLLIDVNE
VEVLEWKLDTNQIHDLVVYHDSIYALHG---

---hqfke*hqknvfsqiflyhqnh*wyxtsvkdptlaasttrkvikxkviktalpqdqq
lngtnntatlstsppqsivsssttatxatatstsigaptevkrtvivkkkiikkaaalst
tttttpqissppisnptpsppxyvetvss

Frame C:
h*iiiinnnkilyilyq*qiqyhq*tqslfqy*qah*pnr*l*ifky*qlpmlvky*vsf
klvrnnvrilnrvlvfthfinplfthqdlakdyg*piqlmvvfyrl*ishhpqpqtkmvl
qiqyqi*y*nqnh*ylqihpmmqilislvnsq*ysqsyyhlvit*yhmmnyhys*smlmk
lkf*ngn*iqikfmtwlfimiqsmhymv---

---INSKSSIKRMYFLRYSFIIRTINGIXQVLKIQL*lhqqlerl*rxrl*kqhyhrinn
*meqiiqqlyqlhhhnq*yhhqqqqxqqqqhqhq*ehqqkskeqll*rkkllkkqrhyql
qlqphhkshrhqfqilhhhhpnm*kpyhh
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFK294 (VFK294Q) /CSM/VF/VFK2-D/VFK294Q.Seq.d/                       1641   0.0
CHH523 (CHH523Q) /CSM/CH/CHH5-A/CHH523Q.Seq.d/                       1063   0.0
SFH356 (SFH356Q) /CSM/SF/SFH3-C/SFH356Q.Seq.d/                        993   0.0
VHL309 (VHL309Q) /CSM/VH/VHL3-A/VHL309Q.Seq.d/                        494   e-138
VFJ386 (VFJ386Q) /CSM/VF/VFJ3-D/VFJ386Q.Seq.d/                        446   e-124
VHM791 (VHM791Q) /CSM/VH/VHM7-D/VHM791Q.Seq.d/                        430   e-119
SHK834 (SHK834Q) /CSM/SH/SHK8-B/SHK834Q.Seq.d/                        430   e-119
SHJ625 (SHJ625Q) /CSM/SH/SHJ6-B/SHJ625Q.Seq.d/                        430   e-119
CHS417 (CHS417Q) /CSM/CH/CHS4-A/CHS417Q.Seq.d/                        430   e-119
AHF529 (AHF529Q) /CSM/AH/AHF5-B/AHF529Q.Seq.d/                        430   e-119
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116984) Dictyostelium discoideum chromosome 2 map 2567470...  1241   0.0    1
(BJ415231) Dictyostelium discoideum cDNA clone:ddv21k24, 5' ...  1241   0.0    1
(BJ365953) Dictyostelium discoideum cDNA clone:ddc37m05, 5' ...  1063   0.0    1
(BJ400987) Dictyostelium discoideum cDNA clone:dds15p13, 3' ...   993   0.0    1
(BJ433443) Dictyostelium discoideum cDNA clone:ddv21k24, 3' ...   416   e-173  3
(BJ443464) Dictyostelium discoideum cDNA clone:ddv53b03, 3' ...   266   e-163  4
(BJ350345) Dictyostelium discoideum cDNA clone:dda39d17, 3' ...   266   e-163  4
(BJ397159) Dictyostelium discoideum cDNA clone:dds45d10, 5' ...   266   e-163  4
(BJ371438) Dictyostelium discoideum cDNA clone:ddc58b06, 5' ...   266   e-163  4
(BJ333343) Dictyostelium discoideum cDNA clone:dda42i07, 5' ...   266   e-154  4
dna update 2009. 2. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK129265_1(AK129265|pid:none) Mus musculus premature mRNA for mK...    45   0.003
(P59438) RecName: Full=Hermansky-Pudlak syndrome 5 protein homol...    45   0.003
AF534398_1(AF534398|pid:none) Mus musculus HPS5 protein isoform ...    45   0.003
AF534399_1(AF534399|pid:none) Mus musculus HPS5 protein isoform ...    45   0.003
AY089625_1(AY089625|pid:none) Drosophila melanogaster RE38137 fu...    43   0.021
(Q297N8) RecName: Full=Hermansky-Pudlak syndrome 5 protein homol...    41   0.063
AK291663_1(AK291663|pid:none) Homo sapiens cDNA FLJ78666 complet...    41   0.082
AB463066_1(AB463066|pid:none) Synthetic construct DNA, clone: pF...    41   0.082
(Q9UPZ3) RecName: Full=Hermansky-Pudlak syndrome 5 protein; AltN...    41   0.082
AF534402_1(AF534402|pid:none) Homo sapiens HPS5 protein minor fo...    41   0.082
protein update 2009. 6.22
PSORT 

 psg: 0.87  gvh: 0.56  alm: 0.36  top: 0.43  tms: 0.00  mit: 0.44  mip: 0.15
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: mitochondrial
	 12.0 %: cytoplasmic
	 12.0 %: nuclear
	  8.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vacuolar

>> prediction for VFK294 is mit
5' end seq. ID VFK294F
5' end seq. 
>VFK294F.Seq
GTCATTAAATAATCATCATCAACAACAACAAAATATTGTACATACTATACCAATAACAAA
TTCAATACCACCAGTAAACTCAATCACTATTTCAATATTAGCAAGCACATTAACCAAATC
GATAGTTGTAAATTTTCAAATACTAACAGCTACCAATGTTGGTAAAGTATTGAGTATCAT
TCAAATTGGTAAGAAACAACGTGAGAATACTAAACAGGGTGCTTGTTTTCACCCATTTTA
TAAATCCTCTGTTTACACATCAAGACCTGGCAAAAGATTATGGTTAGCCGATCCAATTGA
TGGTCGTGTTTTATCGACTATGAATTTCACACCATCCTCAACCACAAACGAAGATGGTAC
TACAAATCCAATACCAAATATAATATTGAAACCAAAACCATTAATATCTTCAAATTCATC
CAATGATGCAAATACTTATCTCACTGGTAAATTCCCAATGATATTCTCAAAGTTATTACC
ATTTGGTAATTACTTAATATCATATGATGAATTATCATTACTCTTAATCGATGTTAATGA
AGTTGAAGTTTTAGAATGGAAATTAGATACAAATCAAATTCATGACTTGGTTGTTTATCA
TGATTCAATCTATGCATTACATGGTCNNNNNNNNNN
Length of 5' end seq. 636
3' end seq. ID VFK294Z
3' end seq. 
>VFK294Z.Seq
NNNNNNNNNNACATCAATTCAAAGAGTAGCATCAAAAGAATGTATTTTCTCAGATATTCC
TTTATCATCAGAACCATTAATGGTATCANACAAGTGTTAAAGATCCAACTTTAGCTGCAT
CAACAACTAGAAAGGTTATAAAGAANAAGGTTATAAAAACAGCATTACCACAGGATCAAC
AATTAAATGGAACAAATAATACAGCAACTCTATCAACTTCACCACCACAATCAATAGTAT
CATCATCAACAACAGCAACANCAGCAACAGCAACATCAACATCAATAGGAGCACCAACAG
AAGTCAAAAGAACAGTTATTGTAAAGAAAAAAATTATTAAAAAAGCAGCGGCATTATCAA
CTACAACTACAACCACACCACAAATCTCATCGCCACCAATTTCAAATCCTACACCATCAC
CACCCNAATATGTAGAAACCGTATCATCAT
Length of 3' end seq. 450
Connected seq. ID VFK294P
Connected seq. 
>VFK294P.Seq
GTCATTAAATAATCATCATCAACAACAACAAAATATTGTACATACTATACCAATAACAAA
TTCAATACCACCAGTAAACTCAATCACTATTTCAATATTAGCAAGCACATTAACCAAATC
GATAGTTGTAAATTTTCAAATACTAACAGCTACCAATGTTGGTAAAGTATTGAGTATCAT
TCAAATTGGTAAGAAACAACGTGAGAATACTAAACAGGGTGCTTGTTTTCACCCATTTTA
TAAATCCTCTGTTTACACATCAAGACCTGGCAAAAGATTATGGTTAGCCGATCCAATTGA
TGGTCGTGTTTTATCGACTATGAATTTCACACCATCCTCAACCACAAACGAAGATGGTAC
TACAAATCCAATACCAAATATAATATTGAAACCAAAACCATTAATATCTTCAAATTCATC
CAATGATGCAAATACTTATCTCACTGGTAAATTCCCAATGATATTCTCAAAGTTATTACC
ATTTGGTAATTACTTAATATCATATGATGAATTATCATTACTCTTAATCGATGTTAATGA
AGTTGAAGTTTTAGAATGGAAATTAGATACAAATCAAATTCATGACTTGGTTGTTTATCA
TGATTCAATCTATGCATTACATGGTC----------ACATCAATTCAAAGAGTAGCATCA
AAAGAATGTATTTTCTCAGATATTCCTTTATCATCAGAACCATTAATGGTATCANACAAG
TGTTAAAGATCCAACTTTAGCTGCATCAACAACTAGAAAGGTTATAAAGAANAAGGTTAT
AAAAACAGCATTACCACAGGATCAACAATTAAATGGAACAAATAATACAGCAACTCTATC
AACTTCACCACCACAATCAATAGTATCATCATCAACAACAGCAACANCAGCAACAGCAAC
ATCAACATCAATAGGAGCACCAACAGAAGTCAAAAGAACAGTTATTGTAAAGAAAAAAAT
TATTAAAAAAGCAGCGGCATTATCAACTACAACTACAACCACACCACAAATCTCATCGCC
ACCAATTTCAAATCCTACACCATCACCACCCNAATATGTAGAAACCGTATCATCAT
Length of connected seq. 1066
Full length Seq ID -
Full length Seq. -
Length of full length seq. -