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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFK276
Library VF
(Link to library)
Clone ID VFK276
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16451-1
Original site URL
Representative seq. ID VFK276P
(Link to Original site)
Representative DNA sequence 
>VFK276 (VFK276Q) /CSM/VF/VFK2-D/VFK276Q.Seq.d/
AAATAAAAAAAGCAAAATGCAAAATAAACCATCATTCATTTTAAGAAACCCATCAGCTAA
TAAAGGTACTGGTTTTAATAATGAAGAAAGAGAAAAATTAGGATTAAAAGGTTTATTACC
ACCAAAGGTTGAATCATTACAAGAACAATCAGATAGAGCATTATCACAATTTACATTATT
TAATACAAACTTGGAAAGATATATTTTCTTAAATTGTTTACGTGATCGTAATGAAACTTT
ATTCTATTATTTATTAAGTAATAATTTAGAATTAATGATGCCAATCATTTATACACCAAC
CGTAGGTGAAGCATGTCAAAAGTTTGGTAATGAATTCCGTTTCGCCCAAGGTATGTATTT
TGCATCCCAAGATAAAGGTAACATTCGTGCCATGATGGACAATTGGCCAGCCGAAGGTGT
CGATATTATCGTTGTTTCCGATGGTTCACGTATTTTGGGTTTAGGTGATCTCGGTACCAA
CGGTATGGGTATCCCAGTTGGTAAGTTACAATTGTACGTCGCCGGTGCTGGTTTCTGTCC
AACCCGTACACTCCCAGTCATTATCGATAGTGGTACAAACACCAAGAAATACCTCXXXXX
XXXXXCCTCNCAAAAGAAACAATACGCTCGTGAGGATTACACCTACCAATTGAAATCATT
ATTAGAGGTTGTCCGTGACGTTAAGCCAACCGCTATCATTGGTTTATCAGGTATCGGTGG
TTCATTCTCTCAAGAAGTAATTGAAGAAATGGCCAAACACGTCGAGAAACCAATCGTTTT
CGCTCTCTCAAATCCAACCACCAACGCTGAATGTACCGCAGAACAAGCTTACCAATGGAC
CGATGGTCGTTGTATCTTTGCCTCTGGTTCACCATTCAAACCAGTCGAATACAAAGGTAA
AACCTTTGTTCCAGGTCAAGGTAACAATATGTACATCTTCCCAGGTCTTGGTTTAGCCGC
TAGCGTTTGTGAAGCTAAACATGTCACTGATGCTATGATCATTACTGCCGCTAAAACTCT
TGCCTCCTTTGTAGAAGACTCTGAAGTTCTCACTGGTAAAATCTATCCAGGTCTCCAACA
TATTCGTGAAATCTCAACAAGAATCGCCGTTAAAGTCATTGAAAAAGCTTATGAAGAAGG
TATGGCTCAATTACCACGTCCAGATAATATTGAAGCTTTAGTCAAATCTCGTCAATATGT
TCCATCTTANNATAAATCAAAAAATTNACTATCTTGCCCAATTTCACCAAATACAAATAG
ATTAAGTCCAAAA
sequence update 2001.11.22
Translated Amino Acid sequence 
NKKSKMQNKPSFILRNPSANKGTGFNNEEREKLGLKGLLPPKVESLQEQSDRALSQFTLF
NTNLERYIFLNCLRDRNETLFYYLLSNNLELMMPIIYTPTVGEACQKFGNEFRFAQGMYF
ASQDKGNIRAMMDNWPAEGVDIIVVSDGSRILGLGDLGTNGMGIPVGKLQLYVAGAGFCP
TRTLPVIIDSGTNTKKYL---

---SQKKQYAREDYTYQLKSLLEVVRDVKPTAIIGLSGIGGSFSQEVIEEMAKHVEKPIV
FALSNPTTNAECTAEQAYQWTDGRCIFASGSPFKPVEYKGKTFVPGQGNNMYIFPGLGLA
ASVCEAKHVTDAMIITAAKTLASFVEDSEVLTGKIYPGLQHIREISTRIAVKVIEKAYEE
GMAQLPRPDNIEALVKSRQYVPSXXKSKNXLSCPISPNTNRLSPK
Translated Amino Acid sequence (All Frames) 
Frame A:
k*kkqnak*tiihfkkpis**rywf***rkrkirikrfittkg*iitrtir*siitiyii
*yklgkiyflklft*s**nfillfik**frindanhlytnrr*smskvw**ipfrpryvf
cipr*r*hschdgqlasrrcryyrcfrwftyfgfr*sryqrygypsw*vtivrrrcwfls
npytpshyr*wykhqeip---

---pxkrnntlvritptn*nhy*rlsvtlsqplslvyqvsvvhslkk*lkkwpntsrnqs
fslsqiqpptlnvpqnkltngpmvvvslplvhhsnqsntkvkplfqvkvtictssqvlv*
plafvklnmslml*sllplkllppl*ktlkfslvksiqvsnifvksqqesplkslkklmk
kvwlnyhvqiilkl*snlvnmfhlxinqkixylaqfhqiqid*vq

Frame B:
NKKSKMQNKPSFILRNPSANKGTGFNNEEREKLGLKGLLPPKVESLQEQSDRALSQFTLF
NTNLERYIFLNCLRDRNETLFYYLLSNNLELMMPIIYTPTVGEACQKFGNEFRFAQGMYF
ASQDKGNIRAMMDNWPAEGVDIIVVSDGSRILGLGDLGTNGMGIPVGKLQLYVAGAGFCP
TRTLPVIIDSGTNTKKYL---

---lxketirs*glhlpieiiirgcp*r*anryhwfiryrwfilsrsn*rngqtrretnr
frslksnhqr*myrrtslpmdrwslylclwftiqtsriqr*nlcsrsr*qyvhlprswfs
r*rl*s*tch*cydhycr*nscllcrrl*sshw*nlsrsptys*nlnknrr*sh*ksl*r
rygsittsr*y*sfsqissicsilx*ikkxtilpnftkyk*iksk

Frame C:
ikkakckinhhsf*ethqlikvlvlimkkekn*d*kvyyhqrlnhyknnqiehyhnlhyl
iqtwkdifs*ivyvivmklysiiy*vii*n**cqsfihqp*vkhvkslvmnsvspkvcil
hpkikvtfvp*wtigqpkvsilslfpmvhvfwv*visvptvwvsqlvsynctspvlvsvq
pvhsqslsivvqtprnt---

---SQKKQYAREDYTYQLKSLLEVVRDVKPTAIIGLSGIGGSFSQEVIEEMAKHVEKPIV
FALSNPTTNAECTAEQAYQWTDGRCIFASGSPFKPVEYKGKTFVPGQGNNMYIFPGLGLA
ASVCEAKHVTDAMIITAAKTLASFVEDSEVLTGKIYPGLQHIREISTRIAVKVIEKAYEE
GMAQLPRPDNIEALVKSRQYVPSXXKSKNXLSCPISPNTNRLSPK
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFK276 (VFK276Q) /CSM/VF/VFK2-D/VFK276Q.Seq.d/                       2418   0.0
SLB437 (SLB437Q) /CSM/SL/SLB4-B/SLB437Q.Seq.d/                       1298   0.0
CHB626 (CHB626Q) /CSM/CH/CHB6-B/CHB626Q.Seq.d/                       1294   0.0
CFJ334 (CFJ334Q) /CSM/CF/CFJ3-B/CFJ334Q.Seq.d/                       1283   0.0
SFD205 (SFD205Q) /CSM/SF/SFD2-A/SFD205Q.Seq.d/                       1277   0.0
CFG469 (CFG469Q) /CSM/CF/CFG4-C/CFG469Q.Seq.d/                       1229   0.0
VFI106 (VFI106Q) /CSM/VF/VFI1-A/VFI106Q.Seq.d/                       1211   0.0
CFH782 (CFH782Q) /CSM/CF/CFH7-D/CFH782Q.Seq.d/                       1211   0.0
CHA161 (CHA161Q) /CSM/CH/CHA1-C/CHA161Q.Seq.d/                       1201   0.0
CHA431 (CHA431Q) /CSM/CH/CHA4-B/CHA431Q.Seq.d/                       1162   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ433424) Dictyostelium discoideum cDNA clone:ddv21g20, 3' ...  1300   0.0    1
(AY387644) Dictyostelium discoideum malic enzyme gene, compl...  1298   0.0    1
(AC115680) Dictyostelium discoideum chromosome 2 map 4915084...  1298   0.0    1
(AU033786) Dictyostelium discoideum slug cDNA, clone SLB437.     1298   0.0    1
(BJ377655) Dictyostelium discoideum cDNA clone:ddc25c08, 3' ...  1294   0.0    1
(BJ399704) Dictyostelium discoideum cDNA clone:dds7i02, 3' e...  1277   0.0    1
(BJ431949) Dictyostelium discoideum cDNA clone:ddv17k01, 3' ...  1211   0.0    1
(BJ375598) Dictyostelium discoideum cDNA clone:ddc19d09, 3' ...  1211   0.0    2
(BJ377101) Dictyostelium discoideum cDNA clone:ddc22i15, 3' ...  1191   0.0    2
(BJ374915) Dictyostelium discoideum cDNA clone:ddc16d21, 3' ...  1191   0.0    2
dna update 2009. 2. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP001322_605(CP001322|pid:none) Desulfatibacillum alkenivorans A...   192   2e-47
DQ901690_1(DQ901690|pid:none) Tigriopus californicus haplotype S...   191   6e-47
DQ901654_1(DQ901654|pid:none) Tigriopus californicus haplotype A...   191   6e-47
DQ901663_1(DQ901663|pid:none) Tigriopus californicus haplotype A...   191   6e-47
DQ901691_1(DQ901691|pid:none) Tigriopus californicus haplotype S...   191   6e-47
DQ901669_1(DQ901669|pid:none) Tigriopus californicus haplotype S...   191   6e-47
DQ901657_1(DQ901657|pid:none) Tigriopus californicus haplotype A...   191   6e-47
DQ901666_1(DQ901666|pid:none) Tigriopus californicus haplotype S...   191   6e-47
AY149910_1(AY149910|pid:none) Mastigamoeba balamuthi malic enzym...   189   1e-46
CP001032_3280(CP001032|pid:none) Opitutus terrae PB90-1, complet...   189   2e-46
protein update 2009. 6.22
PSORT 

 psg: 0.68  gvh: 0.55  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 28.0 %: mitochondrial
	 28.0 %: cytoplasmic
	  4.0 %: cytoskeletal

>> prediction for VFK276 is nuc
5' end seq. ID VFK276F
5' end seq. 
>VFK276F.Seq
AAATAAAAAAAGCAAAATGCAAAATAAACCATCATTCATTTTAAGAAACCCATCAGCTAA
TAAAGGTACTGGTTTTAATAATGAAGAAAGAGAAAAATTAGGATTAAAAGGTTTATTACC
ACCAAAGGTTGAATCATTACAAGAACAATCAGATAGAGCATTATCACAATTTACATTATT
TAATACAAACTTGGAAAGATATATTTTCTTAAATTGTTTACGTGATCGTAATGAAACTTT
ATTCTATTATTTATTAAGTAATAATTTAGAATTAATGATGCCAATCATTTATACACCAAC
CGTAGGTGAAGCATGTCAAAAGTTTGGTAATGAATTCCGTTTCGCCCAAGGTATGTATTT
TGCATCCCAAGATAAAGGTAACATTCGTGCCATGATGGACAATTGGCCAGCCGAAGGTGT
CGATATTATCGTTGTTTCCGATGGTTCACGTATTTTGGGTTTAGGTGATCTCGGTACCAA
CGGTATGGGTATCCCAGTTGGTAAGTTACAATTGTACGTCGCCGGTGCTGGTTTCTGTCC
AACCCGTACACTCCCAGTCATTATCGATAGTGGTACAAACACCAAGAAATACCTCNNNNN
NNNNN
Length of 5' end seq. 605
3' end seq. ID VFK276Z
3' end seq. 
>VFK276Z.Seq
NNNNNNNNNNCCTCNCAAAAGAAACAATACGCTCGTGAGGATTACACCTACCAATTGAAA
TCATTATTAGAGGTTGTCCGTGACGTTAAGCCAACCGCTATCATTGGTTTATCAGGTATC
GGTGGTTCATTCTCTCAAGAAGTAATTGAAGAAATGGCCAAACACGTCGAGAAACCAATC
GTTTTCGCTCTCTCAAATCCAACCACCAACGCTGAATGTACCGCAGAACAAGCTTACCAA
TGGACCGATGGTCGTTGTATCTTTGCCTCTGGTTCACCATTCAAACCAGTCGAATACAAA
GGTAAAACCTTTGTTCCAGGTCAAGGTAACAATATGTACATCTTCCCAGGTCTTGGTTTA
GCCGCTAGCGTTTGTGAAGCTAAACATGTCACTGATGCTATGATCATTACTGCCGCTAAA
ACTCTTGCCTCCTTTGTAGAAGACTCTGAAGTTCTCACTGGTAAAATCTATCCAGGTCTC
CAACATATTCGTGAAATCTCAACAAGAATCGCCGTTAAAGTCATTGAAAAAGCTTATGAA
GAAGGTATGGCTCAATTACCACGTCCAGATAATATTGAAGCTTTAGTCAAATCTCGTCAA
TATGTTCCATCTTANNATAAATCAAAAAATTNACTATCTTGCCCAATTTCACCAAATACA
AATAGATTAAGTCCAAAA
Length of 3' end seq. 678
Connected seq. ID VFK276P
Connected seq. 
>VFK276P.Seq
AAATAAAAAAAGCAAAATGCAAAATAAACCATCATTCATTTTAAGAAACCCATCAGCTAA
TAAAGGTACTGGTTTTAATAATGAAGAAAGAGAAAAATTAGGATTAAAAGGTTTATTACC
ACCAAAGGTTGAATCATTACAAGAACAATCAGATAGAGCATTATCACAATTTACATTATT
TAATACAAACTTGGAAAGATATATTTTCTTAAATTGTTTACGTGATCGTAATGAAACTTT
ATTCTATTATTTATTAAGTAATAATTTAGAATTAATGATGCCAATCATTTATACACCAAC
CGTAGGTGAAGCATGTCAAAAGTTTGGTAATGAATTCCGTTTCGCCCAAGGTATGTATTT
TGCATCCCAAGATAAAGGTAACATTCGTGCCATGATGGACAATTGGCCAGCCGAAGGTGT
CGATATTATCGTTGTTTCCGATGGTTCACGTATTTTGGGTTTAGGTGATCTCGGTACCAA
CGGTATGGGTATCCCAGTTGGTAAGTTACAATTGTACGTCGCCGGTGCTGGTTTCTGTCC
AACCCGTACACTCCCAGTCATTATCGATAGTGGTACAAACACCAAGAAATACCTC-----
-----CCTCNCAAAAGAAACAATACGCTCGTGAGGATTACACCTACCAATTGAAATCATT
ATTAGAGGTTGTCCGTGACGTTAAGCCAACCGCTATCATTGGTTTATCAGGTATCGGTGG
TTCATTCTCTCAAGAAGTAATTGAAGAAATGGCCAAACACGTCGAGAAACCAATCGTTTT
CGCTCTCTCAAATCCAACCACCAACGCTGAATGTACCGCAGAACAAGCTTACCAATGGAC
CGATGGTCGTTGTATCTTTGCCTCTGGTTCACCATTCAAACCAGTCGAATACAAAGGTAA
AACCTTTGTTCCAGGTCAAGGTAACAATATGTACATCTTCCCAGGTCTTGGTTTAGCCGC
TAGCGTTTGTGAAGCTAAACATGTCACTGATGCTATGATCATTACTGCCGCTAAAACTCT
TGCCTCCTTTGTAGAAGACTCTGAAGTTCTCACTGGTAAAATCTATCCAGGTCTCCAACA
TATTCGTGAAATCTCAACAAGAATCGCCGTTAAAGTCATTGAAAAAGCTTATGAAGAAGG
TATGGCTCAATTACCACGTCCAGATAATATTGAAGCTTTAGTCAAATCTCGTCAATATGT
TCCATCTTANNATAAATCAAAAAATTNACTATCTTGCCCAATTTCACCAAATACAAATAG
ATTAAGTCCAAAA
Length of connected seq. 1263
Full length Seq ID -
Full length Seq. -
Length of full length seq. -