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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFK273
Library VF
(Link to library)
Clone ID VFK273
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16028-1|Contig-U16311-1
Original site URL
Representative seq. ID VFK273P
(Link to Original site)
Representative DNA sequence 
>VFK273 (VFK273Q) /CSM/VF/VFK2-D/VFK273Q.Seq.d/
AAATCTTTATTAATATTTTTCAAAATAAATAAATAAATAAATTAAAAATGAAAGTTTTAT
CTGCTTTATGTGTTTTATTAGTTAGTGTTGCCACTGCTAAACAACAATTATCTGAACTCC
AATACAGAAATGCTTTCACCAATTGGATGATTGCTCACCAAAGACATTATTCATCAGAAG
AGTTCAATGGTCGTTTCAATATCTTCAAAGCCAATATGGATTACATCAATGAATGGAATA
CCAAAGGTAGTGAAACCGTTTTAGGTTTAAATGTTTTCGCTGATATCACCAACGAAGAAT
ACAGAGCCACCTATTTAGGTACCCCATTCGATGCATCATCACTCGAAATGACTCCATCTG
AAAAAGTATTTGGTGGTGTCCAAGCTAATTCCGTCGATTGGAGAGCTAAAGGTGCTGTCA
CCCCAATCAAAAATCAAGGTGAATGTGGTGGTTGTTGGTCATTCTCAGCAACTGGTGCCA
CTGAAGGTGCTCAATACATTGCCAATGGTGATTCAGATTTAACATCAGTCTCTGAACAAC
AATTAATCGATTGTTCAGGTTCATACGGTAACAACGGTTGTGAAGGTGGTTTAATGACCT
TAGCTTTCGAATACATCATCAATXXXXXXXXXXTGAACCAGCTTGCTCTTCAACTCAATT
GGATCACGGTGTCTTAGCTGTTGGTTTCGGTAGTGGTTCATCTGGTTCTCAATCACAATC
TGCTGGTTCTCAATCACAATCATCAAATAACAATTGGTCAGAATCATCACAATCACAAGA
TTCAAACAGTTGGTCACAATCATCTCAATCTCAATCATCACAAGATTCAAACAGTTGGTC
ACAATCATCTCAATCTCAAGGTTCCAATAGTTTCACTGGTGCTGGTACAGGTTCAGGTTC
AGGTTCCGTCTCTGGTTCAGGTTCAGCCTCTGGTTCAAGTTCATTCTCAGGTTCATCAAA
TGGTGGTAATAGTAACTCTGGTGATTACCCAACTGATGGTAACTATTGGATCGTTAAAAA
CTCATGGGGTCTTGACTGGGGTATTAATGGTTACATTTTAATGAGTAAAGATAAGGATAA
TCAATGTGGTATTGCTACTATGGCTTCAATCCCACAAGCCATTCCAAAATCAAAATNGAA
TTAATTAAAAATTTATAAATTTTATAAAAAAAAAAATACCAAAAAGATTTTTAAAGTT
sequence update 2001.11.22
Translated Amino Acid sequence 
ifinifqnk*INKLKMKVLSALCVLLVSVATAKQQLSELQYRNAFTNWMIAHQRHYSSEE
FNGRFNIFKANMDYINEWNTKGSETVLGLNVFADITNEEYRATYLGTPFDASSLEMTPSE
KVFGGVQANSVDWRAKGAVTPIKNQGECGGCWSFSATGATEGAQYIANGDSDLTSVSEQQ
LIDCSGSYGNNGCEGGLMTLAFEYIIN---

---EPACSSTQLDHGVLAVGFGSGSSGSQSQSAGSQSQSSNNNWSESSQSQDSNSWSQSS
QSQSSQDSNSWSQSSQSQGSNSFTGAGTGSGSGSVSGSGSASGSSSFSGSSNGGNSNSGD
YPTDGNYWIVKNSWGLDWGINGYILMSKDKDNQCGIATMASIPQAIPKSKXN*lkiykfy
kkkntkkifkv
Translated Amino Acid sequence (All Frames) 
Frame A:
kslliffkink*in*k*kfyllyvfy*lvlpllnnnylnsntemlspig*lltkdiihqk
ssmvvsisskpiwitsmngipkvvkpf*v*mfslisptknteppi*vphsmhhhsk*lhl
kkylvvsklipsigelkvlspqskikvnvvvvghsqqlvplkvlntlpmviqi*hqslnn
n*sivqvhtvttvvkvv**p*lsntss---

---*tsllfnsigsrclscwfr*wfiwfsiticwfsitiik*qlvriititrfkqlvtii
sisiitrfkqlvtiisisrfq*fhwcwyrfrfrfrlwfrfslwfkfilrfikww***lw*
lpn*w*lldr*klmgs*lgy*wlhfne*r*g*smwycyygfnptshskikxeliknl*il
*kkkyqkdf*s

Frame B:
nly*yfsk*ink*iknesficfmcfis*cchc*ttii*tpiqkcfhqlddcspktlfirr
vqwsfqylqsqyglhq*meyqr**nrfrfkcfr*yhqrriqshlfrypirciitrndsi*
ksiwwcps*frrles*rcchpnqksr*mwwllvilsnwch*rcsihcqw*frfnisl*tt
inrlfrfir*qrl*rwfndlsfrihhq---

---EPACSSTQLDHGVLAVGFGSGSSGSQSQSAGSQSQSSNNNWSESSQSQDSNSWSQSS
QSQSSQDSNSWSQSSQSQGSNSFTGAGTGSGSGSVSGSGSASGSSSFSGSSNGGNSNSGD
YPTDGNYWIVKNSWGLDWGINGYILMSKDKDNQCGIATMASIPQAIPKSKXN*lkiykfy
kkkntkkifkv

Frame C:
ifinifqnk*INKLKMKVLSALCVLLVSVATAKQQLSELQYRNAFTNWMIAHQRHYSSEE
FNGRFNIFKANMDYINEWNTKGSETVLGLNVFADITNEEYRATYLGTPFDASSLEMTPSE
KVFGGVQANSVDWRAKGAVTPIKNQGECGGCWSFSATGATEGAQYIANGDSDLTSVSEQQ
LIDCSGSYGNNGCEGGLMTLAFEYIIN---

---nqlalqlnwitvs*llvsvvvhlvlnhnllvlnhnhqitigqnhhnhkiqtvghnhl
nlnhhkiqtvghnhlnlkvpivslvlvqvqvqvpslvqvqplvqvhsqvhqmvvivtlvi
tqlmvtigslkthgvltgvlmvtf**vkiriinvvlllwlqshkpfqnqnxin*kfinfi
kkkipkrflk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFK273 (VFK273Q) /CSM/VF/VFK2-D/VFK273Q.Seq.d/                       2147   0.0
VFN539 (VFN539Q) /CSM/VF/VFN5-B/VFN539Q.Seq.d/                       1138   0.0
VFM673 (VFM673Q) /CSM/VF/VFM6-D/VFM673Q.Seq.d/                       1138   0.0
VFK708 (VFK708Q) /CSM/VF/VFK7-A/VFK708Q.Seq.d/                       1138   0.0
VFK277 (VFK277Q) /CSM/VF/VFK2-D/VFK277Q.Seq.d/                       1138   0.0
VFE296 (VFE296Q) /CSM/VF/VFE2-D/VFE296Q.Seq.d/                       1138   0.0
VFE286 (VFE286Q) /CSM/VF/VFE2-D/VFE286Q.Seq.d/                       1138   0.0
VFD836 (VFD836Q) /CSM/VF/VFD8-B/VFD836Q.Seq.d/                       1138   0.0
VFA125 (VFA125Q) /CSM/VF/VFA1-B/VFA125Q.Seq.d/                       1138   0.0
VHH841 (VHH841Q) /CSM/VH/VHH8-B/VHH841Q.Seq.d/                       1134   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(U72745) Dictyostelium discoideum cysteine proteinase (cprF)...  1138   0.0    1
(BJ417680) Dictyostelium discoideum cDNA clone:ddv28m09, 5' ...  1138   0.0    1
(BJ416535) Dictyostelium discoideum cDNA clone:ddv26a20, 5' ...  1138   0.0    2
(BJ415327) Dictyostelium discoideum cDNA clone:ddv22p01, 5' ...  1138   0.0    2
(BJ415220) Dictyostelium discoideum cDNA clone:ddv21i20, 5' ...  1138   0.0    2
(BJ415184) Dictyostelium discoideum cDNA clone:ddv21a20, 5' ...  1138   0.0    2
(BJ412814) Dictyostelium discoideum cDNA clone:ddv9o24, 5' e...  1138   0.0    2
(BJ412793) Dictyostelium discoideum cDNA clone:ddv9k22, 5' e...  1138   0.0    2
(BJ412409) Dictyostelium discoideum cDNA clone:ddv8h10, 5' e...  1138   0.0    2
(BJ410616) Dictyostelium discoideum cDNA clone:ddv1a07, 5' e...  1138   0.0    2
dna update 2009. 2. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q94503) RecName: Full=Cysteine proteinase 6;          EC=3.4.22...   369   e-100
(Q94504) RecName: Full=Cysteine proteinase 7;          EC=3.4.22...   322   2e-86
L36204_1(L36204|pid:none) Dictyostelium discoideum cysteine prot...   282   1e-74
(P54640) RecName: Full=Cysteine proteinase 5;          EC=3.4.22...   260   6e-68
L36205_1(L36205|pid:none) Dictyostelium discoideum cysteine prot...   256   1e-66
(P04989) RecName: Full=Cysteine proteinase 2;          EC=3.4.22...   201   6e-50
EF053509_1(EF053509|pid:none) Acanthamoeba castellanii cysteine ...   172   3e-41
AC117076_20(AC117076|pid:none) Dictyostelium discoideum chromoso...   158   4e-37
BT071400_1(BT071400|pid:none) Picea sitchensis clone WS0287_F07 ...   149   2e-34
JC4848(JC4848) cysteine proteinase (EC 3.4.22.-) - Douglas fir  ...   148   3e-34
protein update 2009. 6.22
PSORT 

 psg: 0.95  gvh: 0.81  alm: 0.49  top: 0.33  tms: 0.00  mit: 0.33  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: extracellular, including cell wall
	 16.0 %: vacuolar
	  8.0 %: cytoplasmic
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: nuclear

>> prediction for VFK273 is exc
5' end seq. ID VFK273F
5' end seq. 
>VFK273F.Seq
AAATCTTTATTAATATTTTTCAAAATAAATAAATAAATAAATTAAAAATGAAAGTTTTAT
CTGCTTTATGTGTTTTATTAGTTAGTGTTGCCACTGCTAAACAACAATTATCTGAACTCC
AATACAGAAATGCTTTCACCAATTGGATGATTGCTCACCAAAGACATTATTCATCAGAAG
AGTTCAATGGTCGTTTCAATATCTTCAAAGCCAATATGGATTACATCAATGAATGGAATA
CCAAAGGTAGTGAAACCGTTTTAGGTTTAAATGTTTTCGCTGATATCACCAACGAAGAAT
ACAGAGCCACCTATTTAGGTACCCCATTCGATGCATCATCACTCGAAATGACTCCATCTG
AAAAAGTATTTGGTGGTGTCCAAGCTAATTCCGTCGATTGGAGAGCTAAAGGTGCTGTCA
CCCCAATCAAAAATCAAGGTGAATGTGGTGGTTGTTGGTCATTCTCAGCAACTGGTGCCA
CTGAAGGTGCTCAATACATTGCCAATGGTGATTCAGATTTAACATCAGTCTCTGAACAAC
AATTAATCGATTGTTCAGGTTCATACGGTAACAACGGTTGTGAAGGTGGTTTAATGACCT
TAGCTTTCGAATACATCATCAATNNNNNNNNNN
Length of 5' end seq. 633
3' end seq. ID VFK273Z
3' end seq. 
>VFK273Z.Seq
NNNNNNNNNNTGAACCAGCTTGCTCTTCAACTCAATTGGATCACGGTGTCTTAGCTGTTG
GTTTCGGTAGTGGTTCATCTGGTTCTCAATCACAATCTGCTGGTTCTCAATCACAATCAT
CAAATAACAATTGGTCAGAATCATCACAATCACAAGATTCAAACAGTTGGTCACAATCAT
CTCAATCTCAATCATCACAAGATTCAAACAGTTGGTCACAATCATCTCAATCTCAAGGTT
CCAATAGTTTCACTGGTGCTGGTACAGGTTCAGGTTCAGGTTCCGTCTCTGGTTCAGGTT
CAGCCTCTGGTTCAAGTTCATTCTCAGGTTCATCAAATGGTGGTAATAGTAACTCTGGTG
ATTACCCAACTGATGGTAACTATTGGATCGTTAAAAACTCATGGGGTCTTGACTGGGGTA
TTAATGGTTACATTTTAATGAGTAAAGATAAGGATAATCAATGTGGTATTGCTACTATGG
CTTCAATCCCACAAGCCATTCCAAAATCAAAATNGAATTAATTAAAAATTTATAAATTTT
ATAAAAAAAAAAATACCAAAAAGATTTTTAAAGTT
Length of 3' end seq. 575
Connected seq. ID VFK273P
Connected seq. 
>VFK273P.Seq
AAATCTTTATTAATATTTTTCAAAATAAATAAATAAATAAATTAAAAATGAAAGTTTTAT
CTGCTTTATGTGTTTTATTAGTTAGTGTTGCCACTGCTAAACAACAATTATCTGAACTCC
AATACAGAAATGCTTTCACCAATTGGATGATTGCTCACCAAAGACATTATTCATCAGAAG
AGTTCAATGGTCGTTTCAATATCTTCAAAGCCAATATGGATTACATCAATGAATGGAATA
CCAAAGGTAGTGAAACCGTTTTAGGTTTAAATGTTTTCGCTGATATCACCAACGAAGAAT
ACAGAGCCACCTATTTAGGTACCCCATTCGATGCATCATCACTCGAAATGACTCCATCTG
AAAAAGTATTTGGTGGTGTCCAAGCTAATTCCGTCGATTGGAGAGCTAAAGGTGCTGTCA
CCCCAATCAAAAATCAAGGTGAATGTGGTGGTTGTTGGTCATTCTCAGCAACTGGTGCCA
CTGAAGGTGCTCAATACATTGCCAATGGTGATTCAGATTTAACATCAGTCTCTGAACAAC
AATTAATCGATTGTTCAGGTTCATACGGTAACAACGGTTGTGAAGGTGGTTTAATGACCT
TAGCTTTCGAATACATCATCAAT----------TGAACCAGCTTGCTCTTCAACTCAATT
GGATCACGGTGTCTTAGCTGTTGGTTTCGGTAGTGGTTCATCTGGTTCTCAATCACAATC
TGCTGGTTCTCAATCACAATCATCAAATAACAATTGGTCAGAATCATCACAATCACAAGA
TTCAAACAGTTGGTCACAATCATCTCAATCTCAATCATCACAAGATTCAAACAGTTGGTC
ACAATCATCTCAATCTCAAGGTTCCAATAGTTTCACTGGTGCTGGTACAGGTTCAGGTTC
AGGTTCCGTCTCTGGTTCAGGTTCAGCCTCTGGTTCAAGTTCATTCTCAGGTTCATCAAA
TGGTGGTAATAGTAACTCTGGTGATTACCCAACTGATGGTAACTATTGGATCGTTAAAAA
CTCATGGGGTCTTGACTGGGGTATTAATGGTTACATTTTAATGAGTAAAGATAAGGATAA
TCAATGTGGTATTGCTACTATGGCTTCAATCCCACAAGCCATTCCAAAATCAAAATNGAA
TTAATTAAAAATTTATAAATTTTATAAAAAAAAAAATACCAAAAAGATTTTTAAAGTT
Length of connected seq. 1188
Full length Seq ID -
Full length Seq. -
Length of full length seq. -