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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFK250
Library VF
(Link to library)
Clone ID VFK250
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13825-1
Original site URL
Representative seq. ID VFK250P
(Link to Original site)
Representative DNA sequence 
>VFK250 (VFK250Q) /CSM/VF/VFK2-C/VFK250Q.Seq.d/
AAATATTAATATAATTAAAAATGTTAAGAATTAACAGAATTTCTAACTTACGTACTTTTG
GTCAACGTTTCTTCTCAACTGAACAACAAGATGTTGTTGTTATTGGTGGTGGACCAGGTG
GTTATGTTGCAGGTATTAAAGCAGGTCAATTAGGTATGAAAGTTACAGTTGTAGAGAAAA
GAGGTAAATTAGGAGGAACATGTTTAAATGTTGGTTGTATTCCATCAAAAGCATTATTGA
ATGCATCACATCTTTATGAAGAAGCCACCACTAAAATGTCCAAATATGGTGTCAAATGTT
CAGGTGTTGAATTAGATTTGGGAGCAATGATGCAATACAAAGATAAATCAGTCAGTGGCT
TAACATCAGGTATTGAAGGTTTATTCAAAAAGAACAAAGTAAAATATGATAAAGGTTTCG
GTAAAATCACTGGCCCAAACACTGTCGAAGTTACATTAAATGACGGTTCCGTTAAAACTA
TCGAAACCAAAAATATTGTCATTGCAACCGGTTCAGAGGTTACATCACTTCCAAATGTTA
ACATCGATGAAGAGTCTATCATCTCTTCAACCGGTGCCTTAGCTCTTAAGAGTGTTCCAA
AGAAATTGATCGTCATTGGTGGTGGTGTCATTGGTTTXXXXXXXXXXAACTAAAGTTACC
TCTGTCGTTAAAAAATCTGATGGTAAGGTTACTGTAACCGTTGAACAAGTTGGTGCTGGT
GGTTTCACTGGTACTCTCGAAGCTGATGCTGTCCTTGTCTCTGTTGGTCGTCGTCCAAAT
ACTAGTGGTCTTGGTCTTGAATCAGTTGGTATCCCAACCGATAAAGCTGGTCGTGTCGAA
GTTGGAGATCACTTTAACACTAAAGTTCCATCAATTTTCGCAATTGGTGATGCCATCCGT
GGTCCAATGCTTGCTCACAAAGCTGAAGAAGAAGGTATTGCTATCATTGAACAAATTCAC
AATGGTGGTGGTCATGTTAACTATGGTGCCATCCCATCCATTATCTACACTCACCCAGAA
GTTGCTTGGGTTGGTAAGACTGAAGAAGAATTACAAAAAGAAGGTATCCAATACAATATT
GGTAGATTCCCATTCGTTGCAAACTCTAGAGCCAAAACAAACGATGATGTCGAAGGTTTC
GTTAAATTCCTCGCCGCTAAAGATTCTGATAGAGTCCTTGGTGCTCATATTATGGGTACC
AATGCTGGTGAATTAATTGGTGAATGTGTTTTAGCTATGGAATATGGTGCCTCATGTGAA
GATATTGCAAGAACTTGTCANGGTCATCCAACTTTATCCGAAGCTGTTAAAGAAGC
sequence update 2001.11.22
Translated Amino Acid sequence 
ili*LKMLRINRISNLRTFGQRFFSTEQQDVVVIGGGPGGYVAGIKAGQLGMKVTVVEKR
GKLGGTCLNVGCIPSKALLNASHLYEEATTKMSKYGVKCSGVELDLGAMMQYKDKSVSGL
TSGIEGLFKKNKVKYDKGFGKITGPNTVEVTLNDGSVKTIETKNIVIATGSEVTSLPNVN
IDEESIISSTGALALKSVPKKLIVIGGGVIG---

---TKVTSVVKKSDGKVTVTVEQVGAGGFTGTLEADAVLVSVGRRPNTSGLGLESVGIPT
DKAGRVEVGDHFNTKVPSIFAIGDAIRGPMLAHKAEEEGIAIIEQIHNGGGHVNYGAIPS
IIYTHPEVAWVGKTEEELQKEGIQYNIGRFPFVANSRAKTNDDVEGFVKFLAAKDSDRVL
GAHIMGTNAGELIGECVLAMEYGASCEDIARTCXGHPTLSEAVKE
Translated Amino Acid sequence (All Frames) 
Frame A:
ky*yn*kc*eltefltyvllvnvssqlnnkmllllvvdqvvmlqvlkqvn*v*klql*rk
evn*eehv*mlvvfhqkhy*mhhifmkkpplkcpnmvsnvqvln*iweq*cntkinqsva
*hqvlkvyskrtk*nmikvsvkslaqtlsklh*mtvplklskpkilslqpvqrlhhfqml
tsmkslsslqpvp*llrvfqrn*sslvvvslv---

---n*sylcr*ki*w*gycnr*tswcwwfhwysrs*ccpclcwsssky*wsws*iswypn
r*swscrswrsl*h*ssinfrnw*chpwsnacsqs*rrrycyh*tnsqwwwsc*lwchpi
hylhsprsclgw*d*rritkrrypiqyw*ipirckl*sqnkr*crrfr*iprr*rf**sp
wcsyygyqcw*inw*mcfsygiwclm*rycknlsxssnfirsc*rs

Frame B:
niniiknvkn*qnf*ltyfwstflln*ttrcccywwwtrwlccry*srsiryesyscrek
r*irrnmfkcwlysiksiiecitsl*rshh*nvqiwcqmfrc*irfgsndaiqr*isqwl
niry*rfiqkeqski**rfr*nhwpkhcrsyik*rfr*nyrnqkychcnrfrgyitskc*
hr*rvyhlfnrclss*ecskeidrhwwwchwf---

---TKVTSVVKKSDGKVTVTVEQVGAGGFTGTLEADAVLVSVGRRPNTSGLGLESVGIPT
DKAGRVEVGDHFNTKVPSIFAIGDAIRGPMLAHKAEEEGIAIIEQIHNGGGHVNYGAIPS
IIYTHPEVAWVGKTEEELQKEGIQYNIGRFPFVANSRAKTNDDVEGFVKFLAAKDSDRVL
GAHIMGTNAGELIGECVLAMEYGASCEDIARTCXGHPTLSEAVKE

Frame C:
ili*LKMLRINRISNLRTFGQRFFSTEQQDVVVIGGGPGGYVAGIKAGQLGMKVTVVEKR
GKLGGTCLNVGCIPSKALLNASHLYEEATTKMSKYGVKCSGVELDLGAMMQYKDKSVSGL
TSGIEGLFKKNKVKYDKGFGKITGPNTVEVTLNDGSVKTIETKNIVIATGSEVTSLPNVN
IDEESIISSTGALALKSVPKKLIVIGGGVIG---

---lklplslknlmvrll*plnklvlvvslvlsklmlslsllvvvqilvvlvlnqlvsqp
iklvvskleitltlkfhqfsqlvmpsvvqclltklkkkvllslnkftmvvvmltmvpshp
lstltqkllglvrlkknykkkvsntilvdshslqtlepkqtmmskvslnssplkiliesl
vlilwvpmlvn*lvnvf*lwnmvphvkilqelvxviqlypkllkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFK250 (VFK250Q) /CSM/VF/VFK2-C/VFK250Q.Seq.d/                       2543   0.0
VFB625 (VFB625Q) /CSM/VF/VFB6-B/VFB625Q.Seq.d/                       1320   0.0
AFB294 (AFB294Q) /CSM/AF/AFB2-D/AFB294Q.Seq.d/                       1320   0.0
VFC622 (VFC622Q) /CSM/VF/VFC6-A/VFC622Q.Seq.d/                       1312   0.0
AFD276 (AFD276Q) /CSM/AF/AFD2-D/AFD276Q.Seq.d/                       1312   0.0
AFC230 (AFC230Q) /CSM/AF/AFC2-B/AFC230Q.Seq.d/                       1312   0.0
VFB687 (VFB687Q) /CSM/VF/VFB6-D/VFB687Q.Seq.d/                       1310   0.0
CFG142 (CFG142Q) /CSM/CF/CFG1-B/CFG142Q.Seq.d/                       1310   0.0
CFE639 (CFE639Q) /CSM/CF/CFE6-B/CFE639Q.Seq.d/                       1300   0.0
VFC795 (VFC795Q) /CSM/VF/VFC7-D/VFC795Q.Seq.d/                       1298   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ433343) Dictyostelium discoideum cDNA clone:ddv21c14, 3' ...  1320   0.0    1
(BJ429615) Dictyostelium discoideum cDNA clone:ddv4a08, 3' e...  1320   0.0    1
(BJ340795) Dictyostelium discoideum cDNA clone:dda3k24, 3' e...  1320   0.0    1
(BJ430115) Dictyostelium discoideum cDNA clone:ddv6k06, 3' e...  1312   0.0    1
(BJ341707) Dictyostelium discoideum cDNA clone:dda7g20, 3' e...  1312   0.0    1
(BJ341135) Dictyostelium discoideum cDNA clone:dda5k08, 3' e...  1312   0.0    1
(BJ430345) Dictyostelium discoideum cDNA clone:ddv6n23, 3' e...  1298   0.0    1
(BJ430662) Dictyostelium discoideum cDNA clone:ddv8n03, 3' e...  1294   0.0    1
(BJ398352) Dictyostelium discoideum cDNA clone:dds12j05, 3' ...  1294   0.0    1
(BJ342090) Dictyostelium discoideum cDNA clone:dda9a13, 3' e...  1294   0.0    1
dna update 2009. 2. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY466389_1(AY466389|pid:none) Dictyostelium discoideum dihydroli...   303   9e-81
AY391421_1(AY391421|pid:none) Danio rerio dihydrolipoamide dehyd...   254   4e-66
AF542528_19(AF542528|pid:none) Cryptococcus neoformans var. grub...   253   1e-65
Z82277_3(Z82277|pid:none) Caenorhabditis elegans Cosmid LLC1, co...   253   1e-65
AY710429_12(AY710429|pid:none) Cryptococcus gattii strain E566 M...   253   1e-65
AE017344_576(AE017344|pid:none) Cryptococcus neoformans var. neo...   252   2e-65
BT045027_1(BT045027|pid:none) Salmo salar clone ssal-rgf-510-070...   252   2e-65
AY710430_14(AY710430|pid:none) Cryptococcus gattii strain WM276 ...   251   4e-65
AP007171_417(AP007171|pid:none) Aspergillus oryzae RIB40 genomic...   249   2e-64
CR954211_302(CR954211|pid:none) Ostreococcus tauri strain OTTH05...   246   1e-63
protein update 2009. 6.22
PSORT 

 psg: 0.71  gvh: 0.36  alm: 0.32  top: 0.53  tms: 0.00  mit: 0.46  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: mitochondrial
	 28.0 %: cytoplasmic
	 16.0 %: nuclear

>> prediction for VFK250 is mit
5' end seq. ID VFK250F
5' end seq. 
>VFK250F.Seq
AAATATTAATATAATTAAAAATGTTAAGAATTAACAGAATTTCTAACTTACGTACTTTTG
GTCAACGTTTCTTCTCAACTGAACAACAAGATGTTGTTGTTATTGGTGGTGGACCAGGTG
GTTATGTTGCAGGTATTAAAGCAGGTCAATTAGGTATGAAAGTTACAGTTGTAGAGAAAA
GAGGTAAATTAGGAGGAACATGTTTAAATGTTGGTTGTATTCCATCAAAAGCATTATTGA
ATGCATCACATCTTTATGAAGAAGCCACCACTAAAATGTCCAAATATGGTGTCAAATGTT
CAGGTGTTGAATTAGATTTGGGAGCAATGATGCAATACAAAGATAAATCAGTCAGTGGCT
TAACATCAGGTATTGAAGGTTTATTCAAAAAGAACAAAGTAAAATATGATAAAGGTTTCG
GTAAAATCACTGGCCCAAACACTGTCGAAGTTACATTAAATGACGGTTCCGTTAAAACTA
TCGAAACCAAAAATATTGTCATTGCAACCGGTTCAGAGGTTACATCACTTCCAAATGTTA
ACATCGATGAAGAGTCTATCATCTCTTCAACCGGTGCCTTAGCTCTTAAGAGTGTTCCAA
AGAAATTGATCGTCATTGGTGGTGGTGTCATTGGTTTNNNNNNNNNN
Length of 5' end seq. 647
3' end seq. ID VFK250Z
3' end seq. 
>VFK250Z.Seq
NNNNNNNNNNAACTAAAGTTACCTCTGTCGTTAAAAAATCTGATGGTAAGGTTACTGTAA
CCGTTGAACAAGTTGGTGCTGGTGGTTTCACTGGTACTCTCGAAGCTGATGCTGTCCTTG
TCTCTGTTGGTCGTCGTCCAAATACTAGTGGTCTTGGTCTTGAATCAGTTGGTATCCCAA
CCGATAAAGCTGGTCGTGTCGAAGTTGGAGATCACTTTAACACTAAAGTTCCATCAATTT
TCGCAATTGGTGATGCCATCCGTGGTCCAATGCTTGCTCACAAAGCTGAAGAAGAAGGTA
TTGCTATCATTGAACAAATTCACAATGGTGGTGGTCATGTTAACTATGGTGCCATCCCAT
CCATTATCTACACTCACCCAGAAGTTGCTTGGGTTGGTAAGACTGAAGAAGAATTACAAA
AAGAAGGTATCCAATACAATATTGGTAGATTCCCATTCGTTGCAAACTCTAGAGCCAAAA
CAAACGATGATGTCGAAGGTTTCGTTAAATTCCTCGCCGCTAAAGATTCTGATAGAGTCC
TTGGTGCTCATATTATGGGTACCAATGCTGGTGAATTAATTGGTGAATGTGTTTTAGCTA
TGGAATATGGTGCCTCATGTGAAGATATTGCAAGAACTTGTCANGGTCATCCAACTTTAT
CCGAAGCTGTTAAAGAAGC
Length of 3' end seq. 679
Connected seq. ID VFK250P
Connected seq. 
>VFK250P.Seq
AAATATTAATATAATTAAAAATGTTAAGAATTAACAGAATTTCTAACTTACGTACTTTTG
GTCAACGTTTCTTCTCAACTGAACAACAAGATGTTGTTGTTATTGGTGGTGGACCAGGTG
GTTATGTTGCAGGTATTAAAGCAGGTCAATTAGGTATGAAAGTTACAGTTGTAGAGAAAA
GAGGTAAATTAGGAGGAACATGTTTAAATGTTGGTTGTATTCCATCAAAAGCATTATTGA
ATGCATCACATCTTTATGAAGAAGCCACCACTAAAATGTCCAAATATGGTGTCAAATGTT
CAGGTGTTGAATTAGATTTGGGAGCAATGATGCAATACAAAGATAAATCAGTCAGTGGCT
TAACATCAGGTATTGAAGGTTTATTCAAAAAGAACAAAGTAAAATATGATAAAGGTTTCG
GTAAAATCACTGGCCCAAACACTGTCGAAGTTACATTAAATGACGGTTCCGTTAAAACTA
TCGAAACCAAAAATATTGTCATTGCAACCGGTTCAGAGGTTACATCACTTCCAAATGTTA
ACATCGATGAAGAGTCTATCATCTCTTCAACCGGTGCCTTAGCTCTTAAGAGTGTTCCAA
AGAAATTGATCGTCATTGGTGGTGGTGTCATTGGTTT----------AACTAAAGTTACC
TCTGTCGTTAAAAAATCTGATGGTAAGGTTACTGTAACCGTTGAACAAGTTGGTGCTGGT
GGTTTCACTGGTACTCTCGAAGCTGATGCTGTCCTTGTCTCTGTTGGTCGTCGTCCAAAT
ACTAGTGGTCTTGGTCTTGAATCAGTTGGTATCCCAACCGATAAAGCTGGTCGTGTCGAA
GTTGGAGATCACTTTAACACTAAAGTTCCATCAATTTTCGCAATTGGTGATGCCATCCGT
GGTCCAATGCTTGCTCACAAAGCTGAAGAAGAAGGTATTGCTATCATTGAACAAATTCAC
AATGGTGGTGGTCATGTTAACTATGGTGCCATCCCATCCATTATCTACACTCACCCAGAA
GTTGCTTGGGTTGGTAAGACTGAAGAAGAATTACAAAAAGAAGGTATCCAATACAATATT
GGTAGATTCCCATTCGTTGCAAACTCTAGAGCCAAAACAAACGATGATGTCGAAGGTTTC
GTTAAATTCCTCGCCGCTAAAGATTCTGATAGAGTCCTTGGTGCTCATATTATGGGTACC
AATGCTGGTGAATTAATTGGTGAATGTGTTTTAGCTATGGAATATGGTGCCTCATGTGAA
GATATTGCAAGAACTTGTCANGGTCATCCAACTTTATCCGAAGCTGTTAAAGAAGC
Length of connected seq. 1306
Full length Seq ID -
Full length Seq. -
Length of full length seq. -