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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFK248
Library VF
(Link to library)
Clone ID VFK248
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16277-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VFK248 (VFK248Q) /CSM/VF/VFK2-B/VFK248Q.Seq.d/
ATCCCAAGTGTACAATATTAACCTTTTCATACCTTCATCAAAATCACAATATACTTATAT
TTCAAAAATTATAAATAGAATTTTGTAATATTTAGCCAACTTAAATCAAACTAAAAACAA
TTAAAATCAAACCTCAAATTTTTTTATAGATTTATTTATTTTCTCAACCAATCAATCAAT
AAACTACAAATTTATAATATAGATATTTTTTCGAAATAATGACTTCTCCAGCAACAAATA
AATCAGCAGCCTACGATTATTTAATTAAATTACTTTTAATCGGTGATAGTGGTGTAGGTA
AATCATGTCTTTTATTACGTTTTTCTGAAGATTCTTTCACACCAAGTTTCATCACTACTA
TTGGTATCGATTTCAAAATTCGTACAATTGAATTGGAAGGTAAAAGAATTAAATTACAAA
TTTGGGATACTGCAGGTCAAGAAAGATTCAGAACTATCACTACAGCATACTATCGTGGTG
CTATGGGTATCCTATTGGTTTATGATGTCACTGATGAAAAATCTTTTGGTAACATTAGAA
ATTGGATTAGAAATATCGAGCAACATGCTACAGACTCTGTTAATAAAATGTTXXXXXXXX
XX
sequence update 2001.11.22
Translated Amino Acid sequence 
pkctiltfsylhqnhnilifqkl*iefcni*pt*iklktikikpqifl*iylfsqpinq*
ttnl*YRYFFEIMTSPATNKSAAYDYLIKLLLIGDSGVGKSCLLLRFSEDSFTPSFITTI
GIDFKIRTIELEGKRIKLQIWDTAGQERFRTITTAYYRGAMGILLVYDVTDEKSFGNIRN
WIRNIEQHATDSVNKM---
Translated Amino Acid sequence (All Frames) 
Frame A:
ipsvqy*pfhtfikitiylyfknyk*nfvifsqlksn*kqlksnlkffyrfiyflnqsin
klqiynidifsk**llqqqinqqptii*lnyf*svivv*vnhvfyyvflkilshqvssll
lvsiskfvqlnwkvkelnykfgilqvkkdselslqhtivvlwvsywfmmslmknllvtle
igleissnmlqtllikc---


Frame B:
sqvyninlfipssksqytyiskiinril*ylanlnqtknn*nqtsnffidlfifstnqsi
nykfii*iffrnndfssnk*isslrlfn*itfnr**wcr*imsfitff*rffhtkfhhyy
wyrfqnsyn*igr*kn*itnlgycrsrkiqnyhysilswcygypigl*ch**kifw*h*k
ld*kyratcyrlc**n---


Frame C:
pkctiltfsylhqnhnilifqkl*iefcni*pt*iklktikikpqifl*iylfsqpinq*
ttnl*YRYFFEIMTSPATNKSAAYDYLIKLLLIGDSGVGKSCLLLRFSEDSFTPSFITTI
GIDFKIRTIELEGKRIKLQIWDTAGQERFRTITTAYYRGAMGILLVYDVTDEKSFGNIRN
WIRNIEQHATDSVNKM---
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFK248 (VFK248Q) /CSM/VF/VFK2-B/VFK248Q.Seq.d/                       1132   0.0
VFE469 (VFE469Q) /CSM/VF/VFE4-C/VFE469Q.Seq.d/                       1090   0.0
AFJ655 (AFJ655Q) /CSM/AF/AFJ6-C/AFJ655Q.Seq.d/                       1086   0.0
AFN511 (AFN511Q) /CSM/AF/AFN5-A/AFN511Q.Seq.d/                       1084   0.0
SSK334 (SSK334Q) /CSM/SS/SSK3-B/SSK334Q.Seq.d/                        912   0.0
VFH706 (VFH706Q) /CSM/VF/VFH7-A/VFH706Q.Seq.d/                        868   0.0
VFJ353 (VFJ353Q) /CSM/VF/VFJ3-C/VFJ353Q.Seq.d/                        815   0.0
CFB125 (CFB125Q) /CSM/CF/CFB1-B/CFB125Q.Seq.d/                        809   0.0
AFM876 (AFM876Q) /CSM/AF/AFM8-D/AFM876Q.Seq.d/                        706   0.0
SFE866 (SFE866Q) /CSM/SF/SFE8-C/SFE866Q.Seq.d/                        688   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ415110) Dictyostelium discoideum cDNA clone:ddv21o12, 5' ...   886   0.0    2
(BJ412726) Dictyostelium discoideum cDNA clone:ddv9j18, 5' e...   868   0.0    3
(BJ327500) Dictyostelium discoideum cDNA clone:dda20m14, 5' ...   848   0.0    2
(BJ328400) Dictyostelium discoideum cDNA clone:dda28e03, 5' ...   862   0.0    3
(BJ434188) Dictyostelium discoideum cDNA clone:ddv16l01, 3' ...   858   0.0    2
(M34457) Dictyostelium discoideum GTP-binding protein SAS1 g...   837   0.0    1
(BJ414563) Dictyostelium discoideum cDNA clone:ddv19j13, 5' ...   668   0.0    2
(BJ359917) Dictyostelium discoideum cDNA clone:ddc3a07, 5' e...   642   0.0    3
(BJ347346) Dictyostelium discoideum cDNA clone:dda26h20, 3' ...   706   0.0    1
(AC114263) Dictyostelium discoideum chromosome 2 map 215673-...   624   0.0    5
dna update 2009. 2. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P20790) RecName: Full=Ras-related protein Rab-8A; AltName: Full...   249   4e-65
(P20791) RecName: Full=Ras-related protein Rab-8B; AltName: Full...   236   4e-61
GN106009_1(GN106009|pid:none) Sequence 10790 from Patent WO20090...   217   2e-55
AC117265_12(AC117265|pid:none) Oryza sativa (japonica cultivar-g...   217   2e-55
GM977153_1(GM977153|pid:none) Sequence 61 from Patent WO20081456...   217   2e-55
GN105943_1(GN105943|pid:none) Sequence 10724 from Patent WO20090...   217   2e-55
GN106011_1(GN106011|pid:none) Sequence 10792 from Patent WO20090...   217   2e-55
AB079020_1(AB079020|pid:none) Nicotiana tabacum mRNA for ras-rel...   217   2e-55
AC125368_16(AC125368|pid:none) Medicago truncatula clone mth2-13...   217   2e-55
AB015475_6(AB015475|pid:none) Arabidopsis thaliana genomic DNA, ...   217   2e-55
protein update 2009. 6.22
PSORT 

 psg: 0.82  gvh: 0.51  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.36  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.30  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 1.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 28.0 %: mitochondrial
	 16.0 %: nuclear
	  8.0 %: vacuolar
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for VFK248 is cyt
5' end seq. ID VFK248F
5' end seq. 
>VFK248F.Seq
ATCCCAAGTGTACAATATTAACCTTTTCATACCTTCATCAAAATCACAATATACTTATAT
TTCAAAAATTATAAATAGAATTTTGTAATATTTAGCCAACTTAAATCAAACTAAAAACAA
TTAAAATCAAACCTCAAATTTTTTTATAGATTTATTTATTTTCTCAACCAATCAATCAAT
AAACTACAAATTTATAATATAGATATTTTTTCGAAATAATGACTTCTCCAGCAACAAATA
AATCAGCAGCCTACGATTATTTAATTAAATTACTTTTAATCGGTGATAGTGGTGTAGGTA
AATCATGTCTTTTATTACGTTTTTCTGAAGATTCTTTCACACCAAGTTTCATCACTACTA
TTGGTATCGATTTCAAAATTCGTACAATTGAATTGGAAGGTAAAAGAATTAAATTACAAA
TTTGGGATACTGCAGGTCAAGAAAGATTCAGAACTATCACTACAGCATACTATCGTGGTG
CTATGGGTATCCTATTGGTTTATGATGTCACTGATGAAAAATCTTTTGGTAACATTAGAA
ATTGGATTAGAAATATCGAGCAACATGCTACAGACTCTGTTAATAAAATGTTNNNNNNNN
NN
Length of 5' end seq. 602
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -