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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFK243
Library VF
(Link to library)
Clone ID VFK243
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16228-1
Original site URL
Representative seq. ID VFK243P
(Link to Original site)
Representative DNA sequence 
>VFK243 (VFK243Q) /CSM/VF/VFK2-B/VFK243Q.Seq.d/
AAAATTAAAAATAACCCATTAAAGTATCACTACATGTAAATAACAAATTTTATTTGTATT
TCAAAAAAATAATAATTAAAAAAAAAAAAAANTAAAAAAAAAAXXXXXXXXXXATTTCAA
ATTACATCAGATAATTTGNAAGCTGCTAATAAATGTGCAATTTTAATTATTGGTGTATTA
CCAGCACAAGTTCCACAATTATTAGAATCAATTAAACATTTGGTTACCAAGGATCATATT
ATCATTTCAGTTGTATTAGGTATTACAATCAGTGGTATTAGATCCCATCTTCAACCAGAT
GTCTACACACCAATAGTAAGAGCCATGCCAAATACCGCAATTCAATATTGTGAATCCATG
ACATGTATTGCCAACAAGAAGTGACCATCCAACTAAATCAGGTACTGCTGAACAAGAATT
AGCCGATCAAAATGCATTGGAAGTCACTGAAAAGATATTCAATTGTTGTGGTGAATGCAT
TACAATCTCTGAGGAAGCAATGGTATCTGCACCAGCTTTATGCTCATGTGGTACTGCTTT
CTTTTGCAGAATTATTCGTGCTGCTGCAAGTGCAGGTTGCGAAATTGGATTCCACGCTGA
GGATGCTGTTAGAATTGCCGCCCAAACTGCCAAAGGTGCTGCCATCATGTTATTGAAAAA
TGATAGTCATCCAGAATCTGAAATTGATCGTATTACCACTCCAAGTGGTGCCACCATTGC
CGGTTTAAATACTATGGAACATAATGGTTTATCAAGTGCTATCATTAAAGGAATTGTAAT
GTCTGCATCAAAAATCT
sequence update 2001.11.22
Translated Amino Acid sequence 
kiknnplkyhym*itnficiskk**LKKKKXKKK---

---FQITSDNLXAANKCAILIIGVLPAQVPQLLESIKHLVTKDHIIISVVLGITISGIRS
HLQPDVYTPIVRAMPNTAIQYCESMTCIANKK*psn*iryc*trisrskcigsh*kdiql
lw*mhynl*gsngictsfmlmwycfllqnysccckcrlrnwipr*gcc*ncrpncqrcch
hviek**ssri*n*syyhskwchhcrfkyygt*wfikcyh*rncnvcikn
Translated Amino Acid sequence (All Frames) 
Frame A:
kiknnplkyhym*itnficiskk**LKKKKXKKK---

---isnyir*fxsc**mcnfnywcitstsstiirin*tfgyqgsyyhfsciryynqwy*i
psstrclhtnskshakyrnsil*ihdmycqqevtiqlnqvllnkn*pikmhwkslkrysi
vvvnalqslrkqwylhqlyahvvllsfaelfvllqvqvakldstlrmllelppklpkvlp
scy*kmiviqnlklivlplqvvpplpv*ilwnimvyqvlslkel*clhqks

Frame B:
klkith*sittck*qilfvfqknnn*kkkkxkkk---

---FQITSDNLXAANKCAILIIGVLPAQVPQLLESIKHLVTKDHIIISVVLGITISGIRS
HLQPDVYTPIVRAMPNTAIQYCESMTCIANKK*psn*iryc*trisrskcigsh*kdiql
lw*mhynl*gsngictsfmlmwycfllqnysccckcrlrnwipr*gcc*ncrpncqrcch
hviek**ssri*n*syyhskwchhcrfkyygt*wfikcyh*rncnvcikn

Frame C:
n*k*pikvslhvnnkfylyfkkiiikkkkx*kk---

---fklhqiixkllinvqf*llvyyqhkfhny*nqlniwlpriilsfqly*vlqsvvldp
ifnqmsthq**epcqipqfnivnp*hvlptrsdhptksgtaeqeladqnalevtekifnc
cgecitiseeamvsapalcscgtaffcriiraaasagceigfhaedavriaaqtakgaai
mllkndshpeseidrittpsgatiaglntmehnglssaiikgivmsaski
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFK243 (VFK243Q) /CSM/VF/VFK2-B/VFK243Q.Seq.d/                       1350   0.0
VHP477 (VHP477Q) /CSM/VH/VHP4-D/VHP477Q.Seq.d/                       1298   0.0
VFO670 (VFO670Q) /CSM/VF/VFO6-C/VFO670Q.Seq.d/                       1241   0.0
SSC160 (SSC160Q) /CSM/SS/SSC1-C/SSC160Q.Seq.d/                       1227   0.0
VFN356 (VFN356Q) /CSM/VF/VFN3-C/VFN356Q.Seq.d/                       1221   0.0
VSJ175 (VSJ175Q) /CSM/VS/VSJ1-D/VSJ175Q.Seq.d/                       1203   0.0
VFH593 (VFH593Q) /CSM/VF/VFH5-D/VFH593Q.Seq.d/                       1168   0.0
VFM784 (VFM784Q) /CSM/VF/VFM7-D/VFM784Q.Seq.d/                       1072   0.0
VFJ433 (VFJ433Q) /CSM/VF/VFJ4-B/VFJ433Q.Seq.d/                       1068   0.0
VSD684 (VSD684Q) /CSM/VS/VSD6-D/VSD684Q.Seq.d/                       1065   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ433272) Dictyostelium discoideum cDNA clone:ddv21e12, 3' ...  1350   0.0    1
(BJ436274) Dictyostelium discoideum cDNA clone:ddv30k18, 3' ...   795   0.0    2
(BJ446167) Dictyostelium discoideum cDNA clone:ddv61j20, 3' ...   793   0.0    2
(BJ435622) Dictyostelium discoideum cDNA clone:ddv27p13, 3' ...   793   0.0    2
(AU269522) Dictyostelium discoideum vegetative cDNA clone:VS...   743   0.0    2
(BJ434437) Dictyostelium discoideum cDNA clone:ddv16i23, 3' ...   684   0.0    4
(BJ435343) Dictyostelium discoideum cDNA clone:ddv26h21, 3' ...   664   0.0    2
(AU264414) Dictyostelium discoideum vegetative cDNA clone:VS...   787   0.0    2
(BJ432629) Dictyostelium discoideum cDNA clone:ddv19b10, 3' ...   712   0.0    3
(AU268642) Dictyostelium discoideum vegetative cDNA clone:VS...   769   0.0    2
dna update 2009. 2. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55E34) RecName: Full=Pyrroline-5-carboxylate reductase 2;     ...   274   e-118
CU207366_2079(CU207366|pid:none) Gramella forsetii KT0803 comple...   123   1e-39
AM398681_66(AM398681|pid:none) Flavobacterium psychrophilum JIP0...   123   1e-34
CP000685_838(CP000685|pid:none) Flavobacterium johnsoniae UW101,...   118   2e-32
CP000140_2186(CP000140|pid:none) Parabacteroides distasonis ATCC...   102   1e-30
AE015928_3755(AE015928|pid:none) Bacteroides thetaiotaomicron VP...   112   3e-29
CP000139_567(CP000139|pid:none) Bacteroides vulgatus ATCC 8482, ...   105   5e-29
AP006841_530(AP006841|pid:none) Bacteroides fragilis YCH46 DNA, ...   113   8e-24
AP010656_324(AP010656|pid:none) Candidatus Azobacteroides pseudo...    96   8e-24
CP000300_1961(CP000300|pid:none) Methanococcoides burtonii DSM 6...    82   3e-22
protein update 2009. 6.22
PSORT 

 psg: 0.68  gvh: 0.47  alm: 0.30  top: 0.47  tms: 0.07  mit: 0.22  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 24.0 %: cytoplasmic
	 16.0 %: mitochondrial
	 12.0 %: nuclear
	  8.0 %: Golgi
	  8.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VFK243 is end
5' end seq. ID VFK243F
5' end seq. 
>VFK243F.Seq
AAAATTAAAAATAACCCATTAAAGTATCACTACATGTAAATAACAAATTTTATTTGTATT
TCAAAAAAATAATAATTAAAAAAAAAAAAAANTAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 113
3' end seq. ID VFK243Z
3' end seq. 
>VFK243Z.Seq
NNNNNNNNNNATTTCAAATTACATCAGATAATTTGNAAGCTGCTAATAAATGTGCAATTT
TAATTATTGGTGTATTACCAGCACAAGTTCCACAATTATTAGAATCAATTAAACATTTGG
TTACCAAGGATCATATTATCATTTCAGTTGTATTAGGTATTACAATCAGTGGTATTAGAT
CCCATCTTCAACCAGATGTCTACACACCAATAGTAAGAGCCATGCCAAATACCGCAATTC
AATATTGTGAATCCATGACATGTATTGCCAACAAGAAGTGACCATCCAACTAAATCAGGT
ACTGCTGAACAAGAATTAGCCGATCAAAATGCATTGGAAGTCACTGAAAAGATATTCAAT
TGTTGTGGTGAATGCATTACAATCTCTGAGGAAGCAATGGTATCTGCACCAGCTTTATGC
TCATGTGGTACTGCTTTCTTTTGCAGAATTATTCGTGCTGCTGCAAGTGCAGGTTGCGAA
ATTGGATTCCACGCTGAGGATGCTGTTAGAATTGCCGCCCAAACTGCCAAAGGTGCTGCC
ATCATGTTATTGAAAAATGATAGTCATCCAGAATCTGAAATTGATCGTATTACCACTCCA
AGTGGTGCCACCATTGCCGGTTTAAATACTATGGAACATAATGGTTTATCAAGTGCTATC
ATTAAAGGAATTGTAATGTCTGCATCAAAAATCT
Length of 3' end seq. 694
Connected seq. ID VFK243P
Connected seq. 
>VFK243P.Seq
AAAATTAAAAATAACCCATTAAAGTATCACTACATGTAAATAACAAATTTTATTTGTATT
TCAAAAAAATAATAATTAAAAAAAAAAAAAANTAAAAAAAAAA----------ATTTCAA
ATTACATCAGATAATTTGNAAGCTGCTAATAAATGTGCAATTTTAATTATTGGTGTATTA
CCAGCACAAGTTCCACAATTATTAGAATCAATTAAACATTTGGTTACCAAGGATCATATT
ATCATTTCAGTTGTATTAGGTATTACAATCAGTGGTATTAGATCCCATCTTCAACCAGAT
GTCTACACACCAATAGTAAGAGCCATGCCAAATACCGCAATTCAATATTGTGAATCCATG
ACATGTATTGCCAACAAGAAGTGACCATCCAACTAAATCAGGTACTGCTGAACAAGAATT
AGCCGATCAAAATGCATTGGAAGTCACTGAAAAGATATTCAATTGTTGTGGTGAATGCAT
TACAATCTCTGAGGAAGCAATGGTATCTGCACCAGCTTTATGCTCATGTGGTACTGCTTT
CTTTTGCAGAATTATTCGTGCTGCTGCAAGTGCAGGTTGCGAAATTGGATTCCACGCTGA
GGATGCTGTTAGAATTGCCGCCCAAACTGCCAAAGGTGCTGCCATCATGTTATTGAAAAA
TGATAGTCATCCAGAATCTGAAATTGATCGTATTACCACTCCAAGTGGTGCCACCATTGC
CGGTTTAAATACTATGGAACATAATGGTTTATCAAGTGCTATCATTAAAGGAATTGTAAT
GTCTGCATCAAAAATCT
Length of connected seq. 787
Full length Seq ID -
Full length Seq. -
Length of full length seq. -