>VFK218 (VFK218Q) /CSM/VF/VFK2-A/VFK218Q.Seq.d/
XXXXXXXXXXTGTTTAATTGGTGAAGATTTCTTCCATTATTTTATGCATAGATTTTTCCA
TACTCCATGGTTCTATAAAAATATTCATAAGGAACATCCCNCCTATTCAGCACCATTTGG
ATTTACAGCTTCATATGCACATCCAGTTGAAGNTGTATTTTTAGGTATTGCCACATTCGC
TCCAGTTTTAATTATTAGACCNCACTATCNCACTTTTTATTCANGGTTTGTCGTTCGTCA
ATTAGATNCCGTTTTAACTCACTCTGGTTATGATANTGATGTTTTCCCATTCAATTTCAT
GCCAGGGCTANGGTGGTACATCTTTCCATGATTNTCATCATAAAGAGTTTACGGGCAANN
ATGGTTC

---LIGEDFFHYFMHRFFHTPWFYKNIHKEHPXYSAPFGFTASYAHPVEXVFLGIATFAP
VLIIRPHYXTFYSXFVVRQLDXVLTHSGYDXDVFPFNFMPGLXWYIFP*xss*rvygqxw
f

Frame A:
---v*lvkissiilcidfsilhgsikifirnippiqhhldlqlhmhiqlkxyf*vlphsl
qf*lldxtixlfixglsfvn*xpf*ltlvmixmfshsiscqgxggtsfhdxhhkeftgxx
g


Frame B:
---fnw*rflplfya*ifpysmvl*kys*gtsxlfstiwiysfictss*xcifrychirs
sfny*txlshflfxvcrssirxrfnslwl*x*cfpiqfharaxvvhlsmixiikslraxm
v


Frame C:
---LIGEDFFHYFMHRFFHTPWFYKNIHKEHPXYSAPFGFTASYAHPVEXVFLGIATFAP
VLIIRPHYXTFYSXFVVRQLDXVLTHSGYDXDVFPFNFMPGLXWYIFP*xss*rvygqxw
f

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFK218 (VFK218Q) /CSM/VF/VFK2-A/VFK218Q.Seq.d/                        642   0.0
SSJ687 (SSJ687Q) /CSM/SS/SSJ6-D/SSJ687Q.Seq.d/                        196   2e-49
VHF216 (VHF216Q) /CSM/VH/VHF2-A/VHF216Q.Seq.d/                         34   1.4
VFG764 (VFG764Q) /CSM/VF/VFG7-C/VFG764Q.Seq.d/                         34   1.4
VSH582 (VSH582Q) /CSM/VS/VSH5-D/VSH582Q.Seq.d/                         32   5.5
VHQ196 (VHQ196Q) /CSM/VH/VHQ1-D/VHQ196Q.Seq.d/                         32   5.5
VHP794 (VHP794Q) /CSM/VH/VHP7-D/VHP794Q.Seq.d/                         32   5.5
VHP732 (VHP732Q) /CSM/VH/VHP7-B/VHP732Q.Seq.d/                         32   5.5
VHP322 (VHP322Q) /CSM/VH/VHP3-A/VHP322Q.Seq.d/                         32   5.5
VHM252 (VHM252Q) /CSM/VH/VHM2-C/VHM252Q.Seq.d/                         32   5.5

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ433181) Dictyostelium discoideum cDNA clone:ddv21c06, 3' ...   642   e-180  1
(FB336750) Sequence 4276 from Patent WO2007110314.                509   e-158  2
(EC761102) PSE00000917 rw_mgpallid Polysphondylium pallidum ...    86   2e-25  2
(C91149) Dictyostelium discoideum slug cDNA, clone SSJ687.         80   1e-20  2
(ER318771) 1092344162127 Global-Ocean-Sampling_GS-34-01-01-1...    34   0.17   2
(EY662072) CS00-C1-101-040-B03-CT.F Sweet orange leaf, infec...    32   2.4    2
(AP000821) Homo sapiens genomic DNA, chromosome 11 clone:RP1...    44   3.5    1
(AC091496) Homo sapiens chromosome UL clone RP11-199H7, *** ...    44   3.5    1
(AP002359) Homo sapiens genomic DNA, chromosome 11q clone:RP...    44   3.5    1
(AC223960) Bos taurus clone CH240-453J1, WORKING DRAFT SEQUE...    44   3.5    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC126785_23(AC126785|pid:none) Medicago truncatula clone mth2-9i...    98   1e-20
FB334866_1(FB334866|pid:none) Sequence 2392 from Patent WO200711...    96   3e-20
BT055082_1(BT055082|pid:none) Zea mays full-length cDNA clone ZM...    96   3e-20
EU959340_1(EU959340|pid:none) Zea mays clone 217004 C-4 methylst...    96   3e-20
BT037698_1(BT037698|pid:none) Zea mays full-length cDNA clone ZM...    96   3e-20
AY321104_1(AY321104|pid:none) Nicotiana benthamiana C-4 sterol m...    97   3e-20
EU952393_1(EU952393|pid:none) Zea mays clone 1279529 unknown mRNA.     96   3e-20
AP005869_15(AP005869|pid:none) Oryza sativa Japonica Group genom...    96   4e-20
EU180848_13(EU180848|pid:none) Boechera divaricarpa clone BAC45b...    95   9e-20
AF327853_1(AF327853|pid:none) Arabidopsis thaliana sterol 4-alph...    95   9e-20

 psg: 0.75  gvh: 0.49  alm: 0.27  top: 0.73  tms: 0.07  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for VFK218 is nuc

>VFK218Z.Seq
NNNNNNNNNNTGTTTAATTGGTGAAGATTTCTTCCATTATTTTATGCATAGATTTTTCCA
TACTCCATGGTTCTATAAAAATATTCATAAGGAACATCCCNCCTATTCAGCACCATTTGG
ATTTACAGCTTCATATGCACATCCAGTTGAAGNTGTATTTTTAGGTATTGCCACATTCGC
TCCAGTTTTAATTATTAGACCNCACTATCNCACTTTTTATTCANGGTTTGTCGTTCGTCA
ATTAGATNCCGTTTTAACTCACTCTGGTTATGATANTGATGTTTTCCCATTCAATTTCAT
GCCAGGGCTANGGTGGTACATCTTTCCATGATTNTCATCATAAAGAGTTTACGGGCAANN
ATGGTTC