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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFK216
Library VF
(Link to library)
Clone ID VFK216
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10386-1
Original site URL
Representative seq. ID VFK216E
(Link to Original site)
Representative DNA sequence 
>VFK216 (VFK216Q) /CSM/VF/VFK2-A/VFK216Q.Seq.d/
GAAATGAAAGAAAAAACAATGGATGATGATGATATAAAAATTAAATTTGTAAATATAAAA
TCAGAAAATGTGTTTTTAATAGAAACATATTTTAAAGGATTTGAAAAATTACCAAGAAGA
GATTGGGAAAGAGGATTAGAATATATAGTTGGTCAAATGGAAAATGATCATGTAGTAACA
AAAGGTGACTTTGAAATAGATCCACAAACAAATGAAATCACTGTAATATCAAAAGAACGT
GGTATATCATTTGGTGTATCAGCAAACACTGATAAAACATCATCAACCAGATTTCAATTA
GCCAATCCATTTGGTAAAGGTGGTTTAGTTGGTTTTGAATTTAGTGCAGGTCTATATAAA
AATAACAATGGTTCAGTATCATACACTGATAGATTTGGTAATACATTATCTCTTTTAAAA
TCTTCTCAAGATCCAGTTATAAAAGATAGAAATTTTAAAATTGATGAGACTACATTATCC
TATTCATTTCAAAAAAATTCAAATAAATTTTCAATTTTTGCAGGTGATAGTACTGTCTCT
GTTTTAAATAAAAATAAAAACAAAGAACATTTGGCTAATACAGGATTAGCATTTAAAACA
GGTATTTCACATATTTATTCAAGTAATTTAAGAAGTAAATCTATAAATAGACTCTTTAAA
ATTCAAAATGAACTTGCATTTCCAATTCTATCATCCTGTTCATTTTTTAAATCAAATATA
ACATATTCTTTANATTTTCCACTTTACGAACAGTTGAAATTTANAGGTGATTTTANAACT
GGTGGTATTTTTAATTTTAGTAAAGATTCAATGATACCAATATCAGAAAGATTTTTTAAT
GGAAGACATTATAATTTTGAAGGATTTGTTGATTCAAGTTTAACCGAAAAAGGAAGATCT
CCATATTTAGGTAGTAGTTTCTTTTTCACATTTANAACTGCGTTATTACATAAAATTAAA
GATAATGCAAGTGTTATGGCATATCATTGTATTGGTAACNCTGNACTTCCTTTGGATAGT
AATGAAACTACTTTTAAATCAACCGCTCTCAAATTATTCTCAACAAATTCAATTCGTTCT
TCAATTGGTCTTGGTATTTCTGTTGATATGGGTTCTGCCGATATTGAATGTAGTTTGGTT
AAACCATTATTTTTTATTCACAAGATGAACTTAATAATTTTGCATTGGAATTAAAATTAA
AATTNAAAAAAAAAATAAATAAAANATTTAAGAAAA
sequence update 2001.11.24
Translated Amino Acid sequence 
EMKEKTMDDDDIKIKFVNIKSENVFLIETYFKGFEKLPRRDWERGLEYIVGQMENDHVVT
KGDFEIDPQTNEITVISKERGISFGVSANTDKTSSTRFQLANPFGKGGLVGFEFSAGLYK
NNNGSVSYTDRFGNTLSLLKSSQDPVIKDRNFKIDETTLSYSFQKNSNKFSIFAGDSTVS
VLNKNKNKEHLANTGLAFKTGISHIYSSNLRSKSINRLFKIQNELAFPILSSCSFFKSNI
TYSLXFPLYEQLKFXGDFXTGGIFNFSKDSMIPISERFFNGRHYNFEGFVDSSLTEKGRS
PYLGSSFFFTFXTALLHKIKDNASVMAYHCIGNXXLPLDSNETTFKSTALKLFSTNSIRS
SIGLGISVDMGSADIECSLVKPLFFIHKMNLIILHWN*n*nxkkk*ikxlrk
Translated Amino Acid sequence (All Frames) 
Frame A:
EMKEKTMDDDDIKIKFVNIKSENVFLIETYFKGFEKLPRRDWERGLEYIVGQMENDHVVT
KGDFEIDPQTNEITVISKERGISFGVSANTDKTSSTRFQLANPFGKGGLVGFEFSAGLYK
NNNGSVSYTDRFGNTLSLLKSSQDPVIKDRNFKIDETTLSYSFQKNSNKFSIFAGDSTVS
VLNKNKNKEHLANTGLAFKTGISHIYSSNLRSKSINRLFKIQNELAFPILSSCSFFKSNI
TYSLXFPLYEQLKFXGDFXTGGIFNFSKDSMIPISERFFNGRHYNFEGFVDSSLTEKGRS
PYLGSSFFFTFXTALLHKIKDNASVMAYHCIGNXXLPLDSNETTFKSTALKLFSTNSIRS
SIGLGISVDMGSADIECSLVKPLFFIHKMNLIILHWN*n*nxkkk*ikxlrk


Frame B:
k*kkkqwmmmi*klnl*i*nqkmcf**khilkdlknyqeeigked*ni*lvkwkmim**q
kvtlk*ihkqmksl*yqknvvyhlvyqqtlikhhqpdfn*pihlvkvv*lvlnlvqvyik
itmvqyhtlidlvihylf*nllkiql*kieilklmrlhypihfkkiqinfqflqvivlsl
f*ikiktkniwliqd*hlkqvfhifiqvi*evnl*idslkfkmnlhfqfyhpvhflnqi*
hilxifhftns*nlxvilxlvvflilvkiq*yqyqkdflmediiilkdlliqv*pkkedl
hi*vvvsfshlxlryyiklkimqvlwhiivlvtlxflwivmklllnqplsnysqqiqfvl
qlvlvflliwvlpilnvvwlnhyflftr*t**fcigikikixkknk*xi*e


Frame C:
nerknng***ykn*ickykirkcvfnrnif*ri*kitkkrlgkririyswsngk*scsnk
r*l*nrstnk*nhcnikrtwyiiwciskh**niinqisisqsiw*rwfswf*i*crsi*k
*qwfsiih**iw*yiisfkifsrssykr*kf*n**dyiilfiskkfk*ifnfcr**yclc
fk*k*kqrtfg*yrisi*nryftylfk*fkk*iyk*tl*nsk*tcisnsiilfif*ikyn
iffxfstlrtveixr*fxnwwyf*f**rfndtnirkif*wktl*f*ric*fkfnrkrkis
ifr**flfhixncvit*n*r*ckcygislyw*xxtsfg***nyf*inrsqiilnkfnsff
nwswyfc*ygfcry*m*fg*tiifysqdelnnfalelklkxkkkinkxfkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFK216 (VFK216Q) /CSM/VF/VFK2-A/VFK216Q.Seq.d/                       2165   0.0
VFM462 (VFM462Q) /CSM/VF/VFM4-C/VFM462Q.Seq.d/                       1072   0.0
VFE355 (VFE355Q) /CSM/VF/VFE3-C/VFE355Q.Seq.d/                        835   0.0
SSF411 (SSF411Q) /CSM/SS/SSF4-A/SSF411Q.Seq.d/                         50   8e-05
SFL436 (SFL436Q) /CSM/SF/SFL4-B/SFL436Q.Seq.d/                         50   8e-05
SFL384 (SFL384Q) /CSM/SF/SFL3-D/SFL384Q.Seq.d/                         50   8e-05
SFL336 (SFL336Q) /CSM/SF/SFL3-B/SFL336Q.Seq.d/                         50   8e-05
SSK596 (SSK596Q) /CSM/SS/SSK5-D/SSK596Q.Seq.d/                         44   0.005
SHK759 (SHK759Q) /CSM/SH/SHK7-C/SHK759Q.Seq.d/                         44   0.005
SHK255 (SHK255Q) /CSM/SH/SHK2-C/SHK255Q.Seq.d/                         44   0.005
own update 2002.12.18
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ433241) Dictyostelium discoideum cDNA clone:ddv21o04, 3' ...  1243   0.0    1
(BJ434979) Dictyostelium discoideum cDNA clone:ddv25l16, 3' ...  1027   0.0    2
(BJ415026) Dictyostelium discoideum cDNA clone:ddv21o04, 5' ...   781   0.0    2
(BJ412739) Dictyostelium discoideum cDNA clone:ddv9n13, 5' e...   763   0.0    1
(BJ416288) Dictyostelium discoideum cDNA clone:ddv25l16, 5' ...   753   0.0    1
(AF176059) Helcon sp. 16S ribosomal RNA gene, partial sequen...    36   2e-04  3
(AX347077) Sequence 2148 from Patent WO0200928.                    42   0.002  11
(CK422732) AUF_IpSpn_65_i10 Spleen cDNA library Ictalurus pu...    54   0.013  1
(AV790420) Arabidopsis thaliana cDNA clone:RAFL06-88-H21, 3'...    44   0.015  2
(AL193288) Tetraodon nigroviridis genome survey sequence T7 ...    36   0.017  4
dna update 2009. 2. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB170200_1(AB170200|pid:none) Macaca fascicularis brain cDNA clo...    47   0.001
AF151809_1(AF151809|pid:none) Homo sapiens CGI-51 protein mRNA, ...    47   0.001
(Q9Y512) RecName: Full=Sorting and assembly machinery component ...    47   0.001
BC011681_1(BC011681|pid:none) Homo sapiens sorting and assembly ...    47   0.001
(Q6AXV4) RecName: Full=Sorting and assembly machinery component ...    46   0.003
(Q8BGH2) RecName: Full=Sorting and assembly machinery component ...    44   0.009
FM992690_536(FM992690|pid:none) Candida dubliniensis CD36 chromo...    43   0.020
AK159740_1(AK159740|pid:none) Mus musculus osteoclast-like cell ...    42   0.059
CR382135_635(CR382135|pid:none) Debaryomyces hansenii strain CBS...    41   0.10
CR380959_298(CR380959|pid:none) Candida glabrata strain CBS138 c...    40   0.13
protein update 2009. 7.11
PSORT 

 psg: 0.75  gvh: 0.19  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 36.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for VFK216 is nuc
5' end seq. ID VFK216F
5' end seq. 
>VFK216F.Seq
GAAATGAAAGAAAAAACAATGGATGATGATGATATAAAAATTAAATTTGTAAATATAAAA
TCAGAAAATGTGTTTTTAATAGAAACATATTTTAAAGGATTTGAAAAATTACCAAGAAGA
GATTGGGAAAGAGGATTAGAATATATAGTTGGTCAAATGGAAAATGATCATGTAGTAACA
AAAGGTGACTTTGAAATAGATCCACAAACAAATGAAATCACTGTAATATCAAAAGAACGT
GGTATATCATTTGGTGTATCAGCAAACACTGATAAAACATCATCAACCAGATTTCAATTA
GCCAATCCATTTGGTAAAGGTGGTTTAGTTGGTTTTGAATTTAGTGCAGGTCTATATAAA
AATAACAATGGTTCAGTATCATACACTGATAGATTTGGTAATACATTATCTCTTTTAAAA
TCTTCTCAAGATCCAGTTATAAAAGATAGAAATTTTAAAATTGATGAGACTACATTATCC
TATTCATTTCAAAAAAATTCAAATAAATTTTCAATTTTTGCAGGTGATAGTACTGTCTCT
GTTTTAAATAAAAATAAAAACAAAGAACATTTGGCTAATACAGGATTAGCATTTAAAACA
GGTATTTCACATATTTATNNNNNNNNNN
Length of 5' end seq. 628
3' end seq. ID VFK216Z
3' end seq. 
>VFK216Z.Seq
NNNNNNNNNNATAAAAATAAAAACAAAGAACATTTGGCTAATACAGGATTAGCATTTAAA
ACAGGTATTTCACATATTTATTCAAGTAATTTAAGAAGTAAATCTATAAATAGACTCTTT
AAAATTCAAAATGAACTTGCATTTCCAATTCTATCATCCTGTTCATTTTTTAAATCAAAT
ATAACATATTCTTTANATTTTCCACTTTACGAACAGTTGAAATTTANAGGTGATTTTANA
ACTGGTGGTATTTTTAATTTTAGTAAAGATTCAATGATACCAATATCAGAAAGATTTTTT
AATGGAAGACATTATAATTTTGAAGGATTTGTTGATTCAAGTTTAACCGAAAAAGGAAGA
TCTCCATATTTAGGTAGTAGTTTCTTTTTCACATTTANAACTGCGTTATTACATAAAATT
AAAGATAATGCAAGTGTTATGGCATATCATTGTATTGGTAACNCTGNACTTCCTTTGGAT
AGTAATGAAACTACTTTTAAATCAACCGCTCTCAAATTATTCTCAACAAATTCAATTCGT
TCTTCAATTGGTCTTGGTATTTCTGTTGATATGGGTTCTGCCGATATTGAATGTAGTTTG
GTTAAACCATTATTTTTTATTCACAAGATGAACTTAATAATTTTGCATTGGAATTAAAAT
TAAAATTNAAAAAAAAAATAAATAAAANATTTAAGAAAA
Length of 3' end seq. 699
Connected seq. ID VFK216P
Connected seq. 
>VFK216P.Seq
GAAATGAAAGAAAAAACAATGGATGATGATGATATAAAAATTAAATTTGTAAATATAAAA
TCAGAAAATGTGTTTTTAATAGAAACATATTTTAAAGGATTTGAAAAATTACCAAGAAGA
GATTGGGAAAGAGGATTAGAATATATAGTTGGTCAAATGGAAAATGATCATGTAGTAACA
AAAGGTGACTTTGAAATAGATCCACAAACAAATGAAATCACTGTAATATCAAAAGAACGT
GGTATATCATTTGGTGTATCAGCAAACACTGATAAAACATCATCAACCAGATTTCAATTA
GCCAATCCATTTGGTAAAGGTGGTTTAGTTGGTTTTGAATTTAGTGCAGGTCTATATAAA
AATAACAATGGTTCAGTATCATACACTGATAGATTTGGTAATACATTATCTCTTTTAAAA
TCTTCTCAAGATCCAGTTATAAAAGATAGAAATTTTAAAATTGATGAGACTACATTATCC
TATTCATTTCAAAAAAATTCAAATAAATTTTCAATTTTTGCAGGTGATAGTACTGTCTCT
GTTTTAAATAAAAATAAAAACAAAGAACATTTGGCTAATACAGGATTAGCATTTAAAACA
GGTATTTCACATATTTAT----------ATAAAAATAAAAACAAAGAACATTTGGCTAAT
ACAGGATTAGCATTTAAAACAGGTATTTCACATATTTATTCAAGTAATTTAAGAAGTAAA
TCTATAAATAGACTCTTTAAAATTCAAAATGAACTTGCATTTCCAATTCTATCATCCTGT
TCATTTTTTAAATCAAATATAACATATTCTTTANATTTTCCACTTTACGAACAGTTGAAA
TTTANAGGTGATTTTANAACTGGTGGTATTTTTAATTTTAGTAAAGATTCAATGATACCA
ATATCAGAAAGATTTTTTAATGGAAGACATTATAATTTTGAAGGATTTGTTGATTCAAGT
TTAACCGAAAAAGGAAGATCTCCATATTTAGGTAGTAGTTTCTTTTTCACATTTANAACT
GCGTTATTACATAAAATTAAAGATAATGCAAGTGTTATGGCATATCATTGTATTGGTAAC
NCTGNACTTCCTTTGGATAGTAATGAAACTACTTTTAAATCAACCGCTCTCAAATTATTC
TCAACAAATTCAATTCGTTCTTCAATTGGTCTTGGTATTTCTGTTGATATGGGTTCTGCC
GATATTGAATGTAGTTTGGTTAAACCATTATTTTTTATTCACAAGATGAACTTAATAATT
TTGCATTGGAATTAAAATTAAAATTNAAAAAAAAAATAAATAAAANATTTAAGAAAA
Length of connected seq. 1307
Full length Seq ID VFK216E
Full length Seq. 
>VFK216E.Seq
GAAATGAAAGAAAAAACAATGGATGATGATGATATAAAAATTAAATTTGTAAATATAAAA
TCAGAAAATGTGTTTTTAATAGAAACATATTTTAAAGGATTTGAAAAATTACCAAGAAGA
GATTGGGAAAGAGGATTAGAATATATAGTTGGTCAAATGGAAAATGATCATGTAGTAACA
AAAGGTGACTTTGAAATAGATCCACAAACAAATGAAATCACTGTAATATCAAAAGAACGT
GGTATATCATTTGGTGTATCAGCAAACACTGATAAAACATCATCAACCAGATTTCAATTA
GCCAATCCATTTGGTAAAGGTGGTTTAGTTGGTTTTGAATTTAGTGCAGGTCTATATAAA
AATAACAATGGTTCAGTATCATACACTGATAGATTTGGTAATACATTATCTCTTTTAAAA
TCTTCTCAAGATCCAGTTATAAAAGATAGAAATTTTAAAATTGATGAGACTACATTATCC
TATTCATTTCAAAAAAATTCAAATAAATTTTCAATTTTTGCAGGTGATAGTACTGTCTCT
GTTTTAAATAAAAATAAAAACAAAGAACATTTGGCTAATACAGGATTAGCATTTAAAACA
GGTATTTCACATATTTATTCAAGTAATTTAAGAAGTAAATCTATAAATAGACTCTTTAAA
ATTCAAAATGAACTTGCATTTCCAATTCTATCATCCTGTTCATTTTTTAAATCAAATATA
ACATATTCTTTANATTTTCCACTTTACGAACAGTTGAAATTTANAGGTGATTTTANAACT
GGTGGTATTTTTAATTTTAGTAAAGATTCAATGATACCAATATCAGAAAGATTTTTTAAT
GGAAGACATTATAATTTTGAAGGATTTGTTGATTCAAGTTTAACCGAAAAAGGAAGATCT
CCATATTTAGGTAGTAGTTTCTTTTTCACATTTANAACTGCGTTATTACATAAAATTAAA
GATAATGCAAGTGTTATGGCATATCATTGTATTGGTAACNCTGNACTTCCTTTGGATAGT
AATGAAACTACTTTTAAATCAACCGCTCTCAAATTATTCTCAACAAATTCAATTCGTTCT
TCAATTGGTCTTGGTATTTCTGTTGATATGGGTTCTGCCGATATTGAATGTAGTTTGGTT
AAACCATTATTTTTTATTCACAAGATGAACTTAATAATTTTGCATTGGAATTAAAATTAA
AATTNAAAAAAAAAATAAATAAAANATTTAAGAAAA
Length of full length seq. 1236