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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFK215
Library VF
(Link to library)
Clone ID VFK215
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U09694-1
Original site URL
Representative seq. ID VFK215P
(Link to Original site)
Representative DNA sequence 
>VFK215 (VFK215Q) /CSM/VF/VFK2-A/VFK215Q.Seq.d/
ATTCCACAATCTACTTTTTACTACAAAAAAAAAAAAAAAAAAAANGGATTAAAAATTAAA
NTAAANTNTTAAAAAAAANNAAAAAATTTANGGTAANNAAAAAAXXXXXXXXXXTATGGT
AATAATCAATGTTATTATATAAGTGCAGATTTATCAAAAGAGGAAGATTTAAATAATTGT
TTAAAAGAGATTGGTAGAATTTCAAATGATAATGGTCAATATGTGGAAATATTAGTTAAT
TGTGCAGGTGCAGGTAATTGGAAATTCATTGAAGAGACATCGTTTAAGGAATGTAATGAT
ATGATGTCGTTACCCTATTTCGCGGCATTTAATATGACAAGACTGCTATTGGAGCCAATG
NTACTTCATGGCGAGGGCACTATTGTGAATATTAATTCGCCAGTTTCTGTGAATGTTTGG
GGTGGTTGTATTGGGTATGCGTGCTNNAGATGGGCTTTAAAAGGGTTTACAGAATGTTTG
AGAATCNATTTACGTGGCACCGGTGTCAATGTGTTGGAGGTNATACCCGGTGAAACTAAG
TCCGAGTATTTCCAACACAACGGTATCGNCNATGACTCTTTCCCATTACTCAGTAAATTC
GTACCAAAGGTAACAGTTGATCNAGTTGCAAAAGGTACAATCAAAGCAATTGNAAATAGA
AAACAAAAGTTAATCCTACCACTTAATGTTNAATGTTGCAAAATATTTCGCTTTTTTCCC
ATTCTCAATTT
sequence update 2001.11.22
Translated Amino Acid sequence 
stiyfllqkkkkkkxikn*XKXLKKXKKFXVXKK---

---YGNNQCYYISADLSKEEDLNNCLKEIGRISNDNGQYVEILVNCAGAGNWKFIEETSF
KECNDMMSLPYFAAFNMTRLLLEPMXLHGEGTIVNINSPVSVNVWGGCIGYACXRWALKG
FTECLRIXLRGTGVNVLEVIPGETKSEYFQHNGIXXDSFPLLSKFVPKVTVDXVAKGTIK
AIXNRKQKLILPLNVXCCKIFRFFPILN
Translated Amino Acid sequence (All Frames) 
Frame A:
ipqstfyykkkkkkxglkikxxx*kkxknlx*xk---

---YGNNQCYYISADLSKEEDLNNCLKEIGRISNDNGQYVEILVNCAGAGNWKFIEETSF
KECNDMMSLPYFAAFNMTRLLLEPMXLHGEGTIVNINSPVSVNVWGGCIGYACXRWALKG
FTECLRIXLRGTGVNVLEVIPGETKSEYFQHNGIXXDSFPLLSKFVPKVTVDXVAKGTIK
AIXNRKQKLILPLNVXCCKIFRFFPILN

Frame B:
fhnllfttkkkkkkxd*klx*xxkkxxkixgxxk---

---mviinvii*vqiyqkrki*iiv*krlvefqmimvnmwky*livqvqvignslkrhrl
rnvmi*crypisrhli*qdcywsqxyfmarall*ilirqfl*mfgvvvlgmraxdgl*kg
lqnv*esiyvapvsmcwrxypvklspsisnttvsxmtlshysvnsyqr*qlixlqkvqsk
qlxienks*syhlmxnvakyfaffpfsi

Frame C:
stiyfllqkkkkkkxikn*XKXLKKXKKFXVXKK---

---w**smllykcrfikrgrfk*lfkrdw*nfk**wsicgnis*lcrcr*leih*rdiv*
gm**ydvvtlfrgi*ydktaiganxtswrghycey*fasfceclgwlywvcvlxmgfkrv
yrmfenxftwhrcqcvggxtr*n*vrvfptqryrx*lfpitq*irtkgns*ssckrynqs
nxk*ktkvnptt*cxmlqnislfshsqf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFK215 (VFK215Q) /CSM/VF/VFK2-A/VFK215Q.Seq.d/                       1164   0.0
VSK442 (VSK442Q) /CSM/VS/VSK4-B/VSK442Q.Seq.d/                       1140   0.0
VSJ187 (VSJ187Q) /CSM/VS/VSJ1-D/VSJ187Q.Seq.d/                         38   0.18
AHI607 (AHI607Q) /CSM/AH/AHI6-A/AHI607Q.Seq.d/                         36   0.72
VSG222 (VSG222Q) /CSM/VS/VSG2-A/VSG222Q.Seq.d/                         34   2.9
VSE328 (VSE328Q) /CSM/VS/VSE3-B/VSE328Q.Seq.d/                         34   2.9
VSE327 (VSE327Q) /CSM/VS/VSE3-B/VSE327Q.Seq.d/                         34   2.9
VHP273 (VHP273Q) /CSM/VH/VHP2-D/VHP273Q.Seq.d/                         34   2.9
VHO586 (VHO586Q) /CSM/VH/VHO5-D/VHO586Q.Seq.d/                         34   2.9
VHM478 (VHM478Q) /CSM/VH/VHM4-D/VHM478Q.Seq.d/                         34   2.9
own update 2004. 8.30
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ433232) Dictyostelium discoideum cDNA clone:ddv21m04, 3' ...  1164   0.0    1
(AU271205) Dictyostelium discoideum vegetative cDNA clone:VS...   904   0.0    2
(AU271204) Dictyostelium discoideum vegetative cDNA clone:VS...   482   e-132  1
(BJ415016) Dictyostelium discoideum cDNA clone:ddv21m04, 5' ...    48   0.46   1
(CT033831) Zebrafish DNA sequence from clone CH211-215K2 in ...    46   1.8    1
(AC140782) Mus musculus BAC clone RP24-417N13 from chromosom...    46   1.8    1
(AC127414) Mus musculus BAC clone RP23-246P24 from chromosom...    46   1.8    1
(AC104184) Homo sapiens chromosome 3 clone RP11-188P20, comp...    46   1.8    1
(AC097638) Homo sapiens chromosome 3 clone RP11-353H3, compl...    46   1.8    1
(AC138808) Homo sapiens chromosome 5 clone RP11-1026L22, WOR...    46   1.8    1
dna update 2009. 2. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000804_1114(CP000804|pid:none) Roseiflexus castenholzii DSM 13...   114   3e-24
CP000686_376(CP000686|pid:none) Roseiflexus sp. RS-1, complete g...   109   8e-23
CP000903_3865(CP000903|pid:none) Bacillus weihenstephanensis KBA...    73   1e-11
AE017194_4170(AE017194|pid:none) Bacillus cereus ATCC 10987, com...    72   2e-11
AE016879_4017(AE016879|pid:none) Bacillus anthracis str. Ames, c...    72   2e-11
CP001407_4063(CP001407|pid:none) Bacillus cereus 03BB102, comple...    70   5e-11
CP000485_3555(CP000485|pid:none) Bacillus thuringiensis str. Al ...    70   5e-11
AE017355_3840(AE017355|pid:none) Bacillus thuringiensis serovar ...    70   7e-11
CP000764_2688(CP000764|pid:none) Bacillus cereus subsp. cytotoxi...    67   6e-10
CP001101_227(CP001101|pid:none) Chlorobium phaeobacteroides BS1,...    65   2e-09
protein update 2009. 6.22
PSORT 

 psg: 0.64  gvh: 0.40  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for VFK215 is cyt
5' end seq. ID VFK215F
5' end seq. 
>VFK215F.Seq
ATTCCACAATCTACTTTTTACTACAAAAAAAAAAAAAAAAAAAANGGATTAAAAATTAAA
NTAAANTNTTAAAAAAAANNAAAAAATTTANGGTAANNAAAAAANNNNNNNNNN
Length of 5' end seq. 114
3' end seq. ID VFK215Z
3' end seq. 
>VFK215Z.Seq
NNNNNNNNNNTATGGTAATAATCAATGTTATTATATAAGTGCAGATTTATCAAAAGAGGA
AGATTTAAATAATTGTTTAAAAGAGATTGGTAGAATTTCAAATGATAATGGTCAATATGT
GGAAATATTAGTTAATTGTGCAGGTGCAGGTAATTGGAAATTCATTGAAGAGACATCGTT
TAAGGAATGTAATGATATGATGTCGTTACCCTATTTCGCGGCATTTAATATGACAAGACT
GCTATTGGAGCCAATGNTACTTCATGGCGAGGGCACTATTGTGAATATTAATTCGCCAGT
TTCTGTGAATGTTTGGGGTGGTTGTATTGGGTATGCGTGCTNNAGATGGGCTTTAAAAGG
GTTTACAGAATGTTTGAGAATCNATTTACGTGGCACCGGTGTCAATGTGTTGGAGGTNAT
ACCCGGTGAAACTAAGTCCGAGTATTTCCAACACAACGGTATCGNCNATGACTCTTTCCC
ATTACTCAGTAAATTCGTACCAAAGGTAACAGTTGATCNAGTTGCAAAAGGTACAATCAA
AGCAATTGNAAATAGAAAACAAAAGTTAATCCTACCACTTAATGTTNAATGTTGCAAAAT
ATTTCGCTTTTTTCCCATTCTCAATTT
Length of 3' end seq. 627
Connected seq. ID VFK215P
Connected seq. 
>VFK215P.Seq
ATTCCACAATCTACTTTTTACTACAAAAAAAAAAAAAAAAAAAANGGATTAAAAATTAAA
NTAAANTNTTAAAAAAAANNAAAAAATTTANGGTAANNAAAAAA----------TATGGT
AATAATCAATGTTATTATATAAGTGCAGATTTATCAAAAGAGGAAGATTTAAATAATTGT
TTAAAAGAGATTGGTAGAATTTCAAATGATAATGGTCAATATGTGGAAATATTAGTTAAT
TGTGCAGGTGCAGGTAATTGGAAATTCATTGAAGAGACATCGTTTAAGGAATGTAATGAT
ATGATGTCGTTACCCTATTTCGCGGCATTTAATATGACAAGACTGCTATTGGAGCCAATG
NTACTTCATGGCGAGGGCACTATTGTGAATATTAATTCGCCAGTTTCTGTGAATGTTTGG
GGTGGTTGTATTGGGTATGCGTGCTNNAGATGGGCTTTAAAAGGGTTTACAGAATGTTTG
AGAATCNATTTACGTGGCACCGGTGTCAATGTGTTGGAGGTNATACCCGGTGAAACTAAG
TCCGAGTATTTCCAACACAACGGTATCGNCNATGACTCTTTCCCATTACTCAGTAAATTC
GTACCAAAGGTAACAGTTGATCNAGTTGCAAAAGGTACAATCAAAGCAATTGNAAATAGA
AAACAAAAGTTAATCCTACCACTTAATGTTNAATGTTGCAAAATATTTCGCTTTTTTCCC
ATTCTCAATTT
Length of connected seq. 721
Full length Seq ID -
Full length Seq. -
Length of full length seq. -