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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFK163
Library VF
(Link to library)
Clone ID VFK163
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16294-1
Original site URL
Representative seq. ID VFK163P
(Link to Original site)
Representative DNA sequence 
>VFK163 (VFK163Q) /CSM/VF/VFK1-C/VFK163Q.Seq.d/
ATTTTATTTTCCAATTGATAAAATTAAATTAAATAAAATGTCATTAAATATTGAAAAAGT
TAAAGAATTTAGTAATAAAGCATGGGATGAAAATATTTTACCAACACTTTCAAAATATAT
TGAAATTCCAAATCAATCACCATTATATGATGCAGAATGGGCAACCAATGGTTATACAGA
AGAAGCAATTCAATTATTAGAGAAATGGATCTTAGCACAAGGTATCAAAGGTATTTCATG
TGAAATTAAAAGAATTGAAGGATTAACACCAATTATTTTCATGGTAGTCGAAGCAACCAA
AACTGAAAAAGTTAAAACTGTACTTTTATATGGTCACATGGATAAACAACCACCACTCAC
TGATGCATGGGATGAAGGATTACATCCATACAAAGCAGTCATCAAGAATAACAAACTCTA
TGGTCGTGGTGGTGCAGATGATGGTTATTCAACATTTGCTTCAATCTCTGCAATCAAAGC
ATTGCAAGAACAATCAATTCCACATGATCGTTATGTAATTATCATTGAAGGTTCCGAAGA
ATCTGGTTCAATCCATTTACCACAATATGTTGAGAAGTTCCAAGCTGAAATTCAAATTCC
ATCATTGGTAGTTTXXXXXXXXXXTAACTGGTGAAGTCGATAAAAGATTACACGTTGAAA
TTCCACAACATCGTTTAGATCAAATCGCAAAATGTGCTGAAATTTTAGGCGAAACCATCT
ATGATGAATTCTCATGGTACAAAAATACCACTCCAGTCACTAAAGATTTAACCCAATTGA
TGATCAACAAAACTTGGTATCCAACTATTTGCATCACCGGTGCTGAAGGTATTCCACACA
CTTCAAATGCTGGTAATGTCATGAGAACTCACACCACCGTTAAAGTTAGTGTTCGTTTAC
CACCATCATTCAAAGCAGCTGATGCATCAACTGTATTAAAAGAAATTTTCGAAACTAATC
CACCATATGGTGCTGAAGTTACCTTCAAAATGGATAAATGTGCTTCAGGTTGGGATTCAC
CACAAACTAGACCAGAATTAGAAAAAGCATTAGGAGAAGCTTCAAATCTTTTCTTTGGCA
AAGACCACGTATACATGGGTGAAGGTGGTTCCATCCCATTCATGAAAATGTTACATGACC
GTTTCAATTGTCAATTTATCGTTACTGGTTTATTAGGTCCAGGTTCAAATGCTCAGGTCC
AAATGAATTCTTAGATATTCCATTTGCCAAGAAATTATTAAGTTGTGTATTATTATTAGA
TGCTCATTCCAAGTT
sequence update 2001.11.22
Translated Amino Acid sequence 
FYFPIDKIKLNKMSLNIEKVKEFSNKAWDENILPTLSKYIEIPNQSPLYDAEWATNGYTE
EAIQLLEKWILAQGIKGISCEIKRIEGLTPIIFMVVEATKTEKVKTVLLYGHMDKQPPLT
DAWDEGLHPYKAVIKNNKLYGRGGADDGYSTFASISAIKALQEQSIPHDRYVIIIEGSEE
SGSIHLPQYVEKFQAEIQIPSLVV---

---TGEVDKRLHVEIPQHRLDQIAKCAEILGETIYDEFSWYKNTTPVTKDLTQLMINKTW
YPTICITGAEGIPHTSNAGNVMRTHTTVKVSVRLPPSFKAADASTVLKEIFETNPPYGAE
VTFKMDKCASGWDSPQTRPELEKALGEASNLFFGKDHVYMGEGGSIPFMKMLHDRFNCQF
IVTGLLGPGSNAQVQMNS*ifhlprny*vvyyy*mlips
Translated Amino Acid sequence (All Frames) 
Frame A:
ilfsn**n*ik*nviky*ks*ri***smg*kyftntfkiy*nsksitii*crmgnqwlyr
rsnsiiremdlstryqryfm*n*kn*rintnyfhgsrsnqn*ks*nctfiwshg*tttth
*cmg*ritsiqsshqe*qtlwswwcr*wlfnicfnlcnqsiartinst*slcnyh*rfrr
iwfnpfttic*evps*nsnsiigs---

---*lvksikdytlkfhniv*iksqnvlkf*akpsmmnshgtkiplqslki*pn**stkl
giqlfaspvlkvfhtlqmlvms*eltpplklvfvyhhhskqlmhqly*kkfsklihhmvl
klpskwinvlqvgihhkldqn*kkh*eklqifslakttytwvkvvpshs*kcymtvsivn
lsllvy*vqvqmlrsk*ilrysicqeiiklciiircsfqv

Frame B:
FYFPIDKIKLNKMSLNIEKVKEFSNKAWDENILPTLSKYIEIPNQSPLYDAEWATNGYTE
EAIQLLEKWILAQGIKGISCEIKRIEGLTPIIFMVVEATKTEKVKTVLLYGHMDKQPPLT
DAWDEGLHPYKAVIKNNKLYGRGGADDGYSTFASISAIKALQEQSIPHDRYVIIIEGSEE
SGSIHLPQYVEKFQAEIQIPSLVV---

---nw*sr*kitr*nsttsfrsnrkmc*nfrrnhl**ilmvqkyhssh*rfnpiddqqnl
vsnylhhrc*rysthfkcw*chenshhr*s*csfttiiqss*cincikrnfrn*stiwc*
sylqng*mcfrlgfttn*trirksirrsfksflwqrprihg*rwfhpihenvt*pfqlsi
yrywfirsrfkcsgpnefldipfakkllscvllldahsk

Frame C:
fifqlikln*ikch*ilkklknlvikhgmkifyqhfqnilkfqinhhymmqngqpmviqk
kqfny*rngs*hkvskvfhvklkelkd*hqlfsw*skqpklkklklyfymvtwinnhhsl
mhgmkdyihtkqssritnsmvvvvqmmviqhllqslqskhcknnqfhmivm*lslkvpkn
lvqsiyhnmlrssklkfkfhhw*f---

---TGEVDKRLHVEIPQHRLDQIAKCAEILGETIYDEFSWYKNTTPVTKDLTQLMINKTW
YPTICITGAEGIPHTSNAGNVMRTHTTVKVSVRLPPSFKAADASTVLKEIFETNPPYGAE
VTFKMDKCASGWDSPQTRPELEKALGEASNLFFGKDHVYMGEGGSIPFMKMLHDRFNCQF
IVTGLLGPGSNAQVQMNS*ifhlprny*vvyyy*mlips
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFK163 (VFK163Q) /CSM/VF/VFK1-C/VFK163Q.Seq.d/                       2341   0.0
AFJ819 (AFJ819Q) /CSM/AF/AFJ8-A/AFJ819Q.Seq.d/                       1291   0.0
CFG835 (CFG835Q) /CSM/CF/CFG8-B/CFG835Q.Seq.d/                       1275   0.0
AFB648 (AFB648Q) /CSM/AF/AFB6-B/AFB648Q.Seq.d/                       1275   0.0
AFL393 (AFL393Q) /CSM/AF/AFL3-D/AFL393Q.Seq.d/                       1267   0.0
SFL182 (SFL182Q) /CSM/SF/SFL1-D/SFL182Q.Seq.d/                       1261   0.0
AFD881 (AFD881Q) /CSM/AF/AFD8-D/AFD881Q.Seq.d/                       1249   0.0
VFB402 (VFB402Q) /CSM/VF/VFB4-A/VFB402Q.Seq.d/                       1247   0.0
AFB802 (AFB802Q) /CSM/AF/AFB8-A/AFB802Q.Seq.d/                       1243   0.0
VFI168 (VFI168Q) /CSM/VF/VFI1-C/VFI168Q.Seq.d/                       1237   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.    40   0.011  9
AL354810|AL354810.8 Human DNA sequence from clone RP11-527N12 on chromosome 13.    38   0.070  10
AL954845|AL954845.20 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone  DKEY-30H3.    40   0.25   8
BI435717|BI435717.1 EST538478 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCCI86 5' sequence, mRNA sequence.    48   0.28   1
BI433279|BI433279.1 EST536040 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCBC62 5' sequence, mRNA sequence.    48   0.28   1
BQ046631|BQ046631.1 EST595749 P. infestans-challenged potato leaf, incompatible reaction Solanum tuberosum cDNA clone BPLI15C18 5' end, mRNA sequence.    48   0.28   1
BI435853|BI435853.1 EST538614 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCCL22 5' sequence, mRNA sequence.    48   0.28   1
BG591390|BG591390.1 EST499232 P. infestans-challenged leaf Solanum tuberosum cDNA clone BPLI8D17 5' sequence, mRNA sequence.    48   0.28   1
BI435340|BI435340.1 EST538101 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCCD08 5' sequence, mRNA sequence.    48   0.28   1
BQ112173|BQ112173.1 EST597749 mixed potato tissues Solanum tuberosum cDNA clone STMCF59 3' end, mRNA sequence.    48   0.28   1
dna update 2003.10.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ384330_1(DQ384330|pid:none) Hartmannella vermiformis succinyl-...   202   2e-50
DQ384331_1(DQ384331|pid:none) Physarum polycephalum succinyl-dia...   195   3e-48
AM743169_1072(AM743169|pid:none) Stenotrophomonas maltophilia K2...   182   9e-46
AM039952_3097(AM039952|pid:none) Xanthomonas campestris pv. vesi...   178   9e-46
AE008923_2905(AE008923|pid:none) Xanthomonas axonopodis pv. citr...   181   2e-45
CP000113_526(CP000113|pid:none) Myxococcus xanthus DK 1622, comp...   177   2e-45
CP001111_978(CP001111|pid:none) Stenotrophomonas maltophilia R55...   182   2e-45
AM746676_157(AM746676|pid:none) Sorangium cellulosum 'So ce 56' ...   180   2e-45
AM502249_240(AM502249|pid:none) Leishmania infantum chromosome 31.    171   5e-41
CP000733_1810(CP000733|pid:none) Coxiella burnetii Dugway 5J108-...   163   8e-41
protein update 2009. 6.22
PSORT 

 psg: 0.70  gvh: 0.29  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VFK163 is cyt
5' end seq. ID VFK163F
5' end seq. 
>VFK163F.Seq
ATTTTATTTTCCAATTGATAAAATTAAATTAAATAAAATGTCATTAAATATTGAAAAAGT
TAAAGAATTTAGTAATAAAGCATGGGATGAAAATATTTTACCAACACTTTCAAAATATAT
TGAAATTCCAAATCAATCACCATTATATGATGCAGAATGGGCAACCAATGGTTATACAGA
AGAAGCAATTCAATTATTAGAGAAATGGATCTTAGCACAAGGTATCAAAGGTATTTCATG
TGAAATTAAAAGAATTGAAGGATTAACACCAATTATTTTCATGGTAGTCGAAGCAACCAA
AACTGAAAAAGTTAAAACTGTACTTTTATATGGTCACATGGATAAACAACCACCACTCAC
TGATGCATGGGATGAAGGATTACATCCATACAAAGCAGTCATCAAGAATAACAAACTCTA
TGGTCGTGGTGGTGCAGATGATGGTTATTCAACATTTGCTTCAATCTCTGCAATCAAAGC
ATTGCAAGAACAATCAATTCCACATGATCGTTATGTAATTATCATTGAAGGTTCCGAAGA
ATCTGGTTCAATCCATTTACCACAATATGTTGAGAAGTTCCAAGCTGAAATTCAAATTCC
ATCATTGGTAGTTTNNNNNNNNNN
Length of 5' end seq. 624
3' end seq. ID VFK163Z
3' end seq. 
>VFK163Z.Seq
NNNNNNNNNNTAACTGGTGAAGTCGATAAAAGATTACACGTTGAAATTCCACAACATCGT
TTAGATCAAATCGCAAAATGTGCTGAAATTTTAGGCGAAACCATCTATGATGAATTCTCA
TGGTACAAAAATACCACTCCAGTCACTAAAGATTTAACCCAATTGATGATCAACAAAACT
TGGTATCCAACTATTTGCATCACCGGTGCTGAAGGTATTCCACACACTTCAAATGCTGGT
AATGTCATGAGAACTCACACCACCGTTAAAGTTAGTGTTCGTTTACCACCATCATTCAAA
GCAGCTGATGCATCAACTGTATTAAAAGAAATTTTCGAAACTAATCCACCATATGGTGCT
GAAGTTACCTTCAAAATGGATAAATGTGCTTCAGGTTGGGATTCACCACAAACTAGACCA
GAATTAGAAAAAGCATTAGGAGAAGCTTCAAATCTTTTCTTTGGCAAAGACCACGTATAC
ATGGGTGAAGGTGGTTCCATCCCATTCATGAAAATGTTACATGACCGTTTCAATTGTCAA
TTTATCGTTACTGGTTTATTAGGTCCAGGTTCAAATGCTCAGGTCCAAATGAATTCTTAG
ATATTCCATTTGCCAAGAAATTATTAAGTTGTGTATTATTATTAGATGCTCATTCCAAGT
T
Length of 3' end seq. 661
Connected seq. ID VFK163P
Connected seq. 
>VFK163P.Seq
ATTTTATTTTCCAATTGATAAAATTAAATTAAATAAAATGTCATTAAATATTGAAAAAGT
TAAAGAATTTAGTAATAAAGCATGGGATGAAAATATTTTACCAACACTTTCAAAATATAT
TGAAATTCCAAATCAATCACCATTATATGATGCAGAATGGGCAACCAATGGTTATACAGA
AGAAGCAATTCAATTATTAGAGAAATGGATCTTAGCACAAGGTATCAAAGGTATTTCATG
TGAAATTAAAAGAATTGAAGGATTAACACCAATTATTTTCATGGTAGTCGAAGCAACCAA
AACTGAAAAAGTTAAAACTGTACTTTTATATGGTCACATGGATAAACAACCACCACTCAC
TGATGCATGGGATGAAGGATTACATCCATACAAAGCAGTCATCAAGAATAACAAACTCTA
TGGTCGTGGTGGTGCAGATGATGGTTATTCAACATTTGCTTCAATCTCTGCAATCAAAGC
ATTGCAAGAACAATCAATTCCACATGATCGTTATGTAATTATCATTGAAGGTTCCGAAGA
ATCTGGTTCAATCCATTTACCACAATATGTTGAGAAGTTCCAAGCTGAAATTCAAATTCC
ATCATTGGTAGTTT----------TAACTGGTGAAGTCGATAAAAGATTACACGTTGAAA
TTCCACAACATCGTTTAGATCAAATCGCAAAATGTGCTGAAATTTTAGGCGAAACCATCT
ATGATGAATTCTCATGGTACAAAAATACCACTCCAGTCACTAAAGATTTAACCCAATTGA
TGATCAACAAAACTTGGTATCCAACTATTTGCATCACCGGTGCTGAAGGTATTCCACACA
CTTCAAATGCTGGTAATGTCATGAGAACTCACACCACCGTTAAAGTTAGTGTTCGTTTAC
CACCATCATTCAAAGCAGCTGATGCATCAACTGTATTAAAAGAAATTTTCGAAACTAATC
CACCATATGGTGCTGAAGTTACCTTCAAAATGGATAAATGTGCTTCAGGTTGGGATTCAC
CACAAACTAGACCAGAATTAGAAAAAGCATTAGGAGAAGCTTCAAATCTTTTCTTTGGCA
AAGACCACGTATACATGGGTGAAGGTGGTTCCATCCCATTCATGAAAATGTTACATGACC
GTTTCAATTGTCAATTTATCGTTACTGGTTTATTAGGTCCAGGTTCAAATGCTCAGGTCC
AAATGAATTCTTAGATATTCCATTTGCCAAGAAATTATTAAGTTGTGTATTATTATTAGA
TGCTCATTCCAAGTT
Length of connected seq. 1265
Full length Seq ID -
Full length Seq. -
Length of full length seq. -