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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFJ888
Library VF
(Link to library)
Clone ID VFJ888
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15659-1
Original site URL
Representative seq. ID VFJ888P
(Link to Original site)
Representative DNA sequence 
>VFJ888 (VFJ888Q) /CSM/VF/VFJ8-D/VFJ888Q.Seq.d/
GTCGTCTGTAATNATAATAATTGTGTAATATATATTTATTTAAAAAGTTATCAAAAAAGA
TTTAGTTAAAAAAATAATCACAAAAATCAAGAATTTAAAATCAAAATCAAATTAAAATTT
TTTAAAAAAAAAACCAAAGCCCAAACCAAAAGNAGNGNAAAAAAAAAXXXXXXXXXXTAG
ATTTTCAGAACCAAGAGTAAAGATTTATGCAGCAGAGATTGTATCAGCATTGGATCATTT
ACATAAACAAGATATTGTATATCGTGATTTGAAACCAGAGAATATTTTATTAGATAGTGA
AGGTCATATTTGTATCACTGATTTTGGGTTATCTAAAAAGATTGAAACAACCGATGGTAC
ATTCACTTTCTGTGGTACACCAGAGTATTTAGCACCAGAGGTATTGAATGGTCATGGTCA
TGGTTGTGCCGTCGATTGGTGGTCTCTTGGAACTCTTCTCTATGAGATGTTAACTGGTTT
ACCACCTTTCTACTCTCAAAATGTATCAACAATGTATCAAAAAATTTTAAATGGTGAATT
AAAAATTCCAACTTATATTTCACCAGAAGCAAAATCATTATTAGAAGGTTTATTAACTAG
AGAAGTTGATAAAAGACTTGGAACTAAAGGTGGTGGTGAAGTTAAACAACATCCATGGTT
CAAAAATATTGACTGGGAGAAATTGGATAGAAAAGAAGTTGAAGTTCACTTTAAACCAAA
AGTTAAATCNGGCACAGATATTAGTCAAATTGATCCAGTTTTCACTCAAGAAAGACCAAT
GGATAGTTTGGTNAAACAAGTGCTTTAGGTATGCTAGGGTAAAGATACAAGCTTNAAGGT
TTCACTTATGAGCNATTCAATCT
sequence update 2001.11.22
Translated Amino Acid sequence 
vvcnxnncviyiylksyqkrfs*KNNHKNQEFKIKIKLKFFKKKTKAQTKXXXKK---

---RFSEPRVKIYAAEIVSALDHLHKQDIVYRDLKPENILLDSEGHICITDFGLSKKIET
TDGTFTFCGTPEYLAPEVLNGHGHGCAVDWWSLGTLLYEMLTGLPPFYSQNVSTMYQKIL
NGELKIPTYISPEAKSLLEGLLTREVDKRLGTKGGGEVKQHPWFKNIDWEKLDRKEVEVH
FKPKVKSGTDISQIDPVFTQERPMDSLVKQVL*vc*gkdtsxkvslmsxsi
Translated Amino Acid sequence (All Frames) 
Frame A:
vvcnxnncviyiylksyqkrfs*KNNHKNQEFKIKIKLKFFKKKTKAQTKXXXKK---

---*ifrtkskdlcsrdcisigsft*trycis*fetreyfir**rsylyh*fwvi*kd*n
nrwyihflwytrvfstrgiewswswlcrrlvvswnssl*dvnwfttfllskcinnvsknf
kw*iknsnlyftrskiiirrfin*rs**ktwn*rww*s*ttsmvqky*lgeig*krs*ss
l*tks*ixhry*sn*ssfhsrktng*fgxtsalgmlg*ryklxgftyexfn

Frame B:
ssvxiiiv*yifi*kvikkdlvkkiitkiknlksksn*nflkkkpkpkpkxxxkk---

---RFSEPRVKIYAAEIVSALDHLHKQDIVYRDLKPENILLDSEGHICITDFGLSKKIET
TDGTFTFCGTPEYLAPEVLNGHGHGCAVDWWSLGTLLYEMLTGLPPFYSQNVSTMYQKIL
NGELKIPTYISPEAKSLLEGLLTREVDKRLGTKGGGEVKQHPWFKNIDWEKLDRKEVEVH
FKPKVKSGTDISQIDPVFTQERPMDSLVKQVL*vc*gkdtsxkvslmsxsi

Frame C:
rl*x**lcniylfkklskki*lkk*sqksri*nqnqikif*kknqspnqkxxkkk---

---dfqnqe*rfmqqrlyqhwiiyinkilyivi*nqrify*ivkvifvslilgylkrlkq
pmvhslsvvhqsi*hqry*mvmvmvvpsiggllelfsmrc*lvyhlstlkmyqqcikkf*
mvn*kfqlifhqkqnhy*kvy*leklikdlelkvvvklnnihgskiltgrnwiekklkft
lnqklnxaqilvkliqfslkkdqwivwxnkcfryarvkiqaxrfhl*aiqs
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFJ888 (VFJ888Q) /CSM/VF/VFJ8-D/VFJ888Q.Seq.d/                       1336   0.0
AFA767 (AFA767Q) /CSM/AF/AFA7-C/AFA767Q.Seq.d/                       1281   0.0
VFK237 (VFK237Q) /CSM/VF/VFK2-B/VFK237Q.Seq.d/                       1277   0.0
VFO624 (VFO624Q) /CSM/VF/VFO6-A/VFO624Q.Seq.d/                       1263   0.0
AFA783 (AFA783Q) /CSM/AF/AFA7-D/AFA783Q.Seq.d/                       1243   0.0
AFL755 (AFL755Q) /CSM/AF/AFL7-C/AFL755Q.Seq.d/                       1233   0.0
AFN641 (AFN641Q) /CSM/AF/AFN6-B/AFN641Q.Seq.d/                       1070   0.0
VFA754 (VFA754Q) /CSM/VF/VFA7-C/VFA754Q.Seq.d/                       1061   0.0
SSC206 (SSC206Q) /CSM/SS/SSC2-A/SSC206Q.Seq.d/                        959   0.0
SSE740 (SSE740Q) /CSM/SS/SSE7-B/SSE740Q.Seq.d/                        886   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ433164) Dictyostelium discoideum cDNA clone:ddv20p22, 3' ...  1336   0.0    1
(BJ433293) Dictyostelium discoideum cDNA clone:ddv21i10, 3' ...  1243   0.0    2
(M59744) D.discoideum protein kinase 2 mRNA, complete cds.       1213   0.0    4
(BJ340535) Dictyostelium discoideum cDNA clone:dda2f17, 3' e...  1213   0.0    3
(BJ340580) Dictyostelium discoideum cDNA clone:dda2f21, 3' e...  1207   0.0    4
(BJ436167) Dictyostelium discoideum cDNA clone:ddv30o06, 3' ...  1205   0.0    3
(BJ346683) Dictyostelium discoideum cDNA clone:dda24n13, 3' ...  1170   0.0    5
(BJ345612) Dictyostelium discoideum cDNA clone:dda28a12, 3' ...  1070   0.0    1
(BJ429187) Dictyostelium discoideum cDNA clone:ddv2l13, 3' e...   876   0.0    5
(C91912) Dictyostelium discoideum slug cDNA, clone SSC206.        920   0.0    3
dna update 2009. 2. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A38578(A38578) protein kinase 2 (EC 2.7.1.-) - slime mold (Dicty...   429   e-119
AB218429_1(AB218429|pid:none) Schizophyllum commune stk-5 mRNA f...   251   2e-65
CR382131_275(CR382131|pid:none) Yarrowia lipolytica strain CLIB1...   236   9e-61
(P18961) RecName: Full=Serine/threonine-protein kinase YPK2/YKR2...   233   5e-60
CR380957_316(CR380957|pid:none) Candida glabrata strain CBS138 c...   231   2e-59
AE017350_106(AE017350|pid:none) Cryptococcus neoformans var. neo...   229   9e-59
L06196_1(L06196|pid:none) Entamoeba histolytica serine/threonine...   226   6e-58
(P12688) RecName: Full=Serine/threonine-protein kinase YPK1;    ...   225   1e-57
M21307_1(M21307|pid:none) Yeast (S.cerevisiae) protein kinase (Y...   224   4e-57
CU633872_113(CU633872|pid:none) Podospora anserina genomic DNA c...   223   5e-57
protein update 2009. 6.22
PSORT 

 psg: 0.65  gvh: 0.22  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.07
 nuc: 0.06  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall

>> prediction for VFJ888 is nuc
5' end seq. ID VFJ888F
5' end seq. 
>VFJ888F.Seq
GTCGTCTGTAATNATAATAATTGTGTAATATATATTTATTTAAAAAGTTATCAAAAAAGA
TTTAGTTAAAAAAATAATCACAAAAATCAAGAATTTAAAATCAAAATCAAATTAAAATTT
TTTAAAAAAAAAACCAAAGCCCAAACCAAAAGNAGNGNAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 177
3' end seq. ID VFJ888Z
3' end seq. 
>VFJ888Z.Seq
NNNNNNNNNNTAGATTTTCAGAACCAAGAGTAAAGATTTATGCAGCAGAGATTGTATCAG
CATTGGATCATTTACATAAACAAGATATTGTATATCGTGATTTGAAACCAGAGAATATTT
TATTAGATAGTGAAGGTCATATTTGTATCACTGATTTTGGGTTATCTAAAAAGATTGAAA
CAACCGATGGTACATTCACTTTCTGTGGTACACCAGAGTATTTAGCACCAGAGGTATTGA
ATGGTCATGGTCATGGTTGTGCCGTCGATTGGTGGTCTCTTGGAACTCTTCTCTATGAGA
TGTTAACTGGTTTACCACCTTTCTACTCTCAAAATGTATCAACAATGTATCAAAAAATTT
TAAATGGTGAATTAAAAATTCCAACTTATATTTCACCAGAAGCAAAATCATTATTAGAAG
GTTTATTAACTAGAGAAGTTGATAAAAGACTTGGAACTAAAGGTGGTGGTGAAGTTAAAC
AACATCCATGGTTCAAAAATATTGACTGGGAGAAATTGGATAGAAAAGAAGTTGAAGTTC
ACTTTAAACCAAAAGTTAAATCNGGCACAGATATTAGTCAAATTGATCCAGTTTTCACTC
AAGAAAGACCAATGGATAGTTTGGTNAAACAAGTGCTTTAGGTATGCTAGGGTAAAGATA
CAAGCTTNAAGGTTTCACTTATGAGCNATTCAATCT
Length of 3' end seq. 696
Connected seq. ID VFJ888P
Connected seq. 
>VFJ888P.Seq
GTCGTCTGTAATNATAATAATTGTGTAATATATATTTATTTAAAAAGTTATCAAAAAAGA
TTTAGTTAAAAAAATAATCACAAAAATCAAGAATTTAAAATCAAAATCAAATTAAAATTT
TTTAAAAAAAAAACCAAAGCCCAAACCAAAAGNAGNGNAAAAAAAAA----------TAG
ATTTTCAGAACCAAGAGTAAAGATTTATGCAGCAGAGATTGTATCAGCATTGGATCATTT
ACATAAACAAGATATTGTATATCGTGATTTGAAACCAGAGAATATTTTATTAGATAGTGA
AGGTCATATTTGTATCACTGATTTTGGGTTATCTAAAAAGATTGAAACAACCGATGGTAC
ATTCACTTTCTGTGGTACACCAGAGTATTTAGCACCAGAGGTATTGAATGGTCATGGTCA
TGGTTGTGCCGTCGATTGGTGGTCTCTTGGAACTCTTCTCTATGAGATGTTAACTGGTTT
ACCACCTTTCTACTCTCAAAATGTATCAACAATGTATCAAAAAATTTTAAATGGTGAATT
AAAAATTCCAACTTATATTTCACCAGAAGCAAAATCATTATTAGAAGGTTTATTAACTAG
AGAAGTTGATAAAAGACTTGGAACTAAAGGTGGTGGTGAAGTTAAACAACATCCATGGTT
CAAAAATATTGACTGGGAGAAATTGGATAGAAAAGAAGTTGAAGTTCACTTTAAACCAAA
AGTTAAATCNGGCACAGATATTAGTCAAATTGATCCAGTTTTCACTCAAGAAAGACCAAT
GGATAGTTTGGTNAAACAAGTGCTTTAGGTATGCTAGGGTAAAGATACAAGCTTNAAGGT
TTCACTTATGAGCNATTCAATCT
Length of connected seq. 853
Full length Seq ID -
Full length Seq. -
Length of full length seq. -