>VFJ685 (VFJ685Q) /CSM/VF/VFJ6-D/VFJ685Q.Seq.d/
AATTGTACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCT
ACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAA
TCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACC
ACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACC
CCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCA
AAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCC
TCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATG
TCAGGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTA
TTTGCTGAAGCCACTGCCGAACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATT
TTAGGTCTTGCTTTCCAATCCATCTCTGTTAATTCAAXXXXXXXXXXCAGGTGCCANCGG
TGGTGAACTCTCATTNGGTTCAATCGATAACNCCAAATACACTGGTGACATTACCTACGT
CCCATTAACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCA
ATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTACATCACTCAT
TGCTGGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAA
TGGTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAACACCTTACCAAATGTTACCATCAC
CGTTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTT
CGGAAANANTNAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGGAAATTTCTG
GATCCTTGGTGATGTTTTCATCTCTGCANTACTATACTGTATTCGATTTTGGTAATAAAC
AAGTTGGTTTCGCAACNGCCATTCAAGGTTAAATTTTTTNAANAAATTTATATTTAAGAT
AG

nct*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGT
TIPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGA
SSTYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAEATAEPGIAFDFAKFDGI
LGLAFQSISVNS---

---GAXGGELSXGSIDNXKYTGDITYVPLTNETYWEFVMDDFAIDGQSAGFCGTTCHAIC
DSGTSLIAGPMADITALNEKLGAVILNGEGVFSDCSVINTLPNVTITVAGREFVLTPKEY
VLEVTEFGXXXCLSGFMGIELNMGNFWILGDVFISAXLYCIRFW**tswfrnxhsrlnfx
xkfifki

Frame A:
nct*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGT
TIPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGA
SSTYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAEATAEPGIAFDFAKFDGI
LGLAFQSISVNS---

---qvpxvvnshxvqsitpntlvtlptsh*ptkpignslwmtllsmvnqlvsvvllvtqf
aiqvhhsllvqwlillpsmkn*vlss*mvkvsslivalstpyqmlpspllvvnlf*lqkn
tf*kllssexxnv*vdlwvss*tweisgslvmfsslxyytvfdfgnkqvgfataiqg*if
xxnlylr*

Frame B:
ivpdlhkrk*nylf*lyf*lllf*lkl*qyh*tsiklqenleeefhkngqtdyllsmlvp
qsqfqilkmlntmvplplvpqvkpsk*fsilvhptcgfhqrnvqslllhviyitnitavp
qahmsptelispsntvvvlcqvlslkipsllvh*llkinyllkplpnqvllsispnsmvf
*vllsnpslliq---

---rcxrw*tlixfnr*xqihw*hylrpinqrnllgiryg*lcyrwsiswflwyylsrnl
rfryithcwsng*yycpq*kircchlkw*rcll*l*ryqhltkcyhhrcws*icfnskri
rfrsy*vrkxxmfewiygyrvkhgkfldpw*cfhlcxtilysilvinklvsqxpfkvkff
xxiyi*d

Frame C:
lyltyikeneityfnfifsyycfsssfnstiklpssfkri*kksstkmvkqiicsqcwyh
npnfrf*rcsilwchyhwyprsslqssfrywfiqlvdsikemsnhcccm*ft*qi*qrcl
khicrqrn*fhhpir*wcyvrfclsrfrhcwfinc*rsiic*shcrtrycfrfrqirwyf
rscfpihlc*f---

---GAXGGELSXGSIDNXKYTGDITYVPLTNETYWEFVMDDFAIDGQSAGFCGTTCHAIC
DSGTSLIAGPMADITALNEKLGAVILNGEGVFSDCSVINTLPNVTITVAGREFVLTPKEY
VLEVTEFGXXXCLSGFMGIELNMGNFWILGDVFISAXLYCIRFW**tswfrnxhsrlnfx
xkfifki

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFJ685 (VFJ685Q) /CSM/VF/VFJ6-D/VFJ685Q.Seq.d/                       2145   0.0
VFN669 (VFN669Q) /CSM/VF/VFN6-C/VFN669Q.Seq.d/                       1136   0.0
VFJ222 (VFJ222Q) /CSM/VF/VFJ2-A/VFJ222Q.Seq.d/                       1136   0.0
VFB255 (VFB255Q) /CSM/VF/VFB2-C/VFB255Q.Seq.d/                       1136   0.0
VFB231 (VFB231Q) /CSM/VF/VFB2-B/VFB231Q.Seq.d/                       1136   0.0
VFJ113 (VFJ113Q) /CSM/VF/VFJ1-A/VFJ113Q.Seq.d/                       1130   0.0
VFA146 (VFA146Q) /CSM/VF/VFA1-B/VFA146Q.Seq.d/                       1130   0.0
VFA140 (VFA140Q) /CSM/VF/VFA1-B/VFA140Q.Seq.d/                       1130   0.0
VFO689 (VFO689Q) /CSM/VF/VFO6-D/VFO689Q.Seq.d/                       1124   0.0
VFN780 (VFN780Q) /CSM/VF/VFN7-D/VFN780Q.Seq.d/                       1124   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

Y16962|Y16962.1 Dictyostelium discoideum mRNA for cathepsin D.   1124   0.0    2
AJ243946|AJ243946.1 Dictyostelium discoideum ctsD gene for cathepsin D, exons 1 to 2.   965   0.0    4
E33916|E33916.1 Candida boidinii strain with lowered protease activity and  utilization thereof as host for producing foreign protein.    38   4e-08  5
E33915|E33915.1 Candida boidinii strain with lowered protease activity and  utilization thereof as host for producing foreign protein.    38   1e-06  5
AB060540|AB060540.1 Candida boidinii PEP4 gene for proteinase A, complete cds.    38   1e-06  5
AF076243|AF076243.1 Arabidopsis thaliana BAC T26N6 from chromosome IV at 19.3 cM, complete sequence.    62   2e-05  3
CB264579|CB264579.1 51-E015023-035-004-F13-T7R MPIZ-ADIS-035 Arabidopsis thaliana cDNA clone MPIZp2000F134Q 5-PRIME, mRNA sequence.    62   2e-05  1
AL765461|AL765461.1 Arabidopsis thaliana T-DNA flanking sequence GK-139E08-012875.    62   2e-05  1
AX059531|AX059531.1 Sequence 264 from Patent WO0055325.            62   2e-05  1
AL762930|AL762930.1 Arabidopsis thaliana T-DNA flanking sequence GK-030C11-011522.    62   2e-05  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ010007_1(DQ010007|pid:none) Bombyx mori CathD mRNA, complete c...   187   1e-83
EF213114_1(EF213114|pid:none) Penaeus monodon cathepsin D mRNA, ...   183   7e-83
(Q03168) RecName: Full=Lysosomal aspartic protease;          EC=...   180   2e-82
FJ168036_1(FJ168036|pid:none) Fasciola hepatica cathepsin D-like...   176   4e-82
AB106552_1(AB106552|pid:none) Todarodes pacificus tpaD mRNA for ...   184   1e-81
BT080419_1(BT080419|pid:none) Caligus clemensi clone ccle-evs-50...   169   3e-81
EF070454_1(EF070454|pid:none) Maconellicoccus hirsutus clone WHM...   181   5e-81
EF000001_1(EF000001|pid:none) Fasciola hepatica cathepsin D-like...   174   5e-80
FJ654712_1(FJ654712|pid:none) Chrysomela tremulae aspartic prote...   187   9e-80
AF420068_1(AF420068|pid:none) Clonorchis sinensis aspartic prote...   173   1e-79

 psg: 0.98  gvh: 0.77  alm: 0.38  top: 0.57  tms: 0.00  mit: 0.36  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: extracellular, including cell wall
	 20.0 %: mitochondrial
	 16.0 %: nuclear
	 12.0 %: vacuolar
	  4.0 %: cytoplasmic
	  4.0 %: cytoskeletal

>> prediction for VFJ685 is exc

>VFJ685F.Seq
AATTGTACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCT
ACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAA
TCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACC
ACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACC
CCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCA
AAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCC
TCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATG
TCAGGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTA
TTTGCTGAAGCCACTGCCGAACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATT
TTAGGTCTTGCTTTCCAATCCATCTCTGTTAATTCAANNNNNNNNNN

>VFJ685Z.Seq
NNNNNNNNNNCAGGTGCCANCGGTGGTGAACTCTCATTNGGTTCAATCGATAACNCCAAA
TACACTGGTGACATTACCTACGTCCCATTAACCAACGAAACCTATTGGGAATTCGTTATG
GATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATT
TGCGATTCAGGTACATCACTCATTGCTGGTCCAATGGCTGATATTACTGCCCTCAATGAA
AAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAAC
ACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGAA
TACGTTTTAGAAGTTACTGAGTTCGGAAANANTNAATGTTTGAGTGGATTTATGGGTATC
GAGTTAAACATGGGAAATTTCTGGATCCTTGGTGATGTTTTCATCTCTGCANTACTATAC
TGTATTCGATTTTGGTAATAAACAAGTTGGTTTCGCAACNGCCATTCAAGGTTAAATTTT
TTNAANAAATTTATATTTAAGATAG

>VFJ685P.Seq
AATTGTACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCT
ACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAA
TCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACC
ACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACC
CCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCA
AAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCC
TCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATG
TCAGGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTA
TTTGCTGAAGCCACTGCCGAACCAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATT
TTAGGTCTTGCTTTCCAATCCATCTCTGTTAATTCAA----------CAGGTGCCANCGG
TGGTGAACTCTCATTNGGTTCAATCGATAACNCCAAATACACTGGTGACATTACCTACGT
CCCATTAACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCA
ATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTACATCACTCAT
TGCTGGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAA
TGGTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAACACCTTACCAAATGTTACCATCAC
CGTTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTT
CGGAAANANTNAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGGAAATTTCTG
GATCCTTGGTGATGTTTTCATCTCTGCANTACTATACTGTATTCGATTTTGGTAATAAAC
AAGTTGGTTTCGCAACNGCCATTCAAGGTTAAATTTTTTNAANAAATTTATATTTAAGAT
AG