>VFJ649 (VFJ649Q) /CSM/VF/VFJ6-C/VFJ649Q.Seq.d/
ATATATAAAAAATGGCATCTATAAATACAGAATCGGATGATTATNTCCAATTGTTATTTT
AATTGATGAATTAAAAAATGAGGATATACAACTTAGACTTAATTCAATAAAAAAACTTCA
ATCAATTGCAAAAGCATTAGGACCAGAACGTACCCGTACAGAATTAATCCCATATTTACA
AGACTCTGTTCTCGAAGATGAAGATGAAGTTTTAGTTGTATTATCAGAAGAATTAGGAAA
TTTAATCGAGTTTGTTGGTGGTGCAGAACATGCTGTTTGTTTATTACCACCATTACAAAT
TTTAGCAGGTGCTGAGGAATTAGTTGTTAGAGAAAAGGCAGTGGAATCATTATGTAAAAT
TGCAAAAGAGATCCCAACATCATCATTCGAAGAATCATTCCTTCCATTATTATTTTCATT
ATCAAAAGCAGATTGGTTTACATCACGTACATCAGCATGTGGACTTTTCACTGTATCATA
TCCAAGAGCAAATGCTGAAATGAAGAAATCTTTAAGAAAAACTTTTGGGGGATTATATCA
CGATGATACACCAATGXXXXXXXXXXGAGCTGCACTTGGCTCCAAGTCATTATGTCATTG
GCACCCAATTTATGGTAAAGAAGATACATTAACTCATCTTTTAGAATTGTTTTTTACATC
TCTTAAAAGATGATTTCCCAGATGTTCGTTTAAATATCATTTCAAAATTAGATCAAGTAA
GTAAAGTAATTGGTATTGAAATGTTATCACAATCTCTATTACCAGCTATCGTTGAATTGG
CTGAAGATCATCAATGGAGAGTAAGATTAGCAATTATTGATTATATTCCACTTTTAGCTT
CACAATTAGGTGTTGAATTCTTTGATGAAAAACTTGGTAATTTATGTATGACTTGGTTAG
GTGATCCAGTATTTTCAATTCGTGAAGCAGCAACTAATAATTTGAAGAAATTAACCGAAG
TATTTGGAGTTGATTGGGCAAAGAATAATATTATTCCAAAAGTTTTATCTTTACACTCTC
ATCCAAACTATCTCTATCGTATGACCACATTATTTTCAATTTCAACCCTTTCAACTGTTG
TTGGAGGTGATGTTATCTCTAGTTCAATGGTTCCTTTATTGGCCAAAATGGTTTCCGATA
AAGTTCCAAATATTAGATTTAATGTAGCTAAAACTTTCCAAACCATTATTCCATTACTCG
ATAGCACAATTGTACAATCAAGAGTCAAACCATACTCGTAAAACTTCANAAGATACNATA
AAGA

yikngiykyrig*LXPIVILIDELKNEDIQLRLNSIKKLQSIAKALGPERTRTELIPYLQ
DSVLEDEDEVLVVLSEELGNLIEFVGGAEHAVCLLPPLQILAGAEELVVREKAVESLCKI
AKEIPTSSFEESFLPLLFSLSKADWFTSRTSACGLFTVSYPRANAEMKKSLRKTFGGLYH
DDTPM---

---AALGSKSLCHWHPIYGKEDTLTHLLELFFTSLKR*fprcsfkyhfkirssk*snwy*
nvitisitsyr*ig*rssmeskisny*lystfsftirc*il**ktw*fmydlvr*ssifn
s*ssn**feeinrsiws*lgke*yysksfiftlssklslsydhiifnfnpfnccwr*cyl
*fngsfigqngfr*ssky*i*cs*nfpnhysitr*hnctiksqtilvklxkix*r

Frame A:
iykkwhl*iqnrmixsncyfn**ikk*gytt*t*fnkktsincksirtrtypyrinpift
rlcsrr*r*sfsciirrirkfnrvcwwcrtcclfittitnfsrc*gisc*rkgsgiim*n
ckrdpniiirriipsiiifiiksrlvyityismwtfhciiskskc*neeifkknfwgiis
r*ytn---

---elhlapshyvigtqfmvkkih*lif*ncflhllkddfpdvrlniiskldqvskvigi
emlsqsllpaivelaedhqwrvrlaiidyipllasqlgveffdeklgnlcmtwlgdpvfs
ireaatnnlkkltevfgvdwaknniipkvlslhshpnylyrmttlfsistlstvvggdvi
sssmvpllakmvsdkvpnirfnvaktfqtiiplldstivqsrvkpys*nfxryxk

Frame B:
yikngiykyrig*LXPIVILIDELKNEDIQLRLNSIKKLQSIAKALGPERTRTELIPYLQ
DSVLEDEDEVLVVLSEELGNLIEFVGGAEHAVCLLPPLQILAGAEELVVREKAVESLCKI
AKEIPTSSFEESFLPLLFSLSKADWFTSRTSACGLFTVSYPRANAEMKKSLRKTFGGLYH
DDTPM---

---sctwlqvimslapnlw*rryinssfrivfyis*kmisqmfv*isfqn*ik*vk*lvl
kcyhnlyyqlslnwlkiinge*d*qlliifhf*lhn*vlnslmknlviyv*lg*viqyfq
fvkqqlii*rn*pkyleligqriilfqkfylytliqtisiv*phyfqfqpfqlllevmls
lvqwflywpkwfpikfqildlm*lklskplfhysiaqlynqesnhtrktsxdtik

Frame C:
i*kmasintesddyxqllf*lmn*kmriynldliq*knfnqlqkh*dqnvpvqn*shiyk
tlfskmkmkf*lyyqkn*ei*ssllvvqnmlfvyyhhykf*qvlrn*llekrqwnhyvkl
qkrsqhhhsknhsfhyyfhyqkqiglhhvhqhvdfslyhiqeqmlk*rnl*ekllgdyit
mihq---

---AALGSKSLCHWHPIYGKEDTLTHLLELFFTSLKR*fprcsfkyhfkirssk*snwy*
nvitisitsyr*ig*rssmeskisny*lystfsftirc*il**ktw*fmydlvr*ssifn
s*ssn**feeinrsiws*lgke*yysksfiftlssklslsydhiifnfnpfnccwr*cyl
*fngsfigqngfr*ssky*i*cs*nfpnhysitr*hnctiksqtilvklxkix*r

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFJ649 (VFJ649Q) /CSM/VF/VFJ6-C/VFJ649Q.Seq.d/                       2387   0.0
SFF359 (SFF359Q) /CSM/SF/SFF3-C/SFF359Q.Seq.d/                       1302   0.0
AFO390 (AFO390Q) /CSM/AF/AFO3-D/AFO390Q.Seq.d/                       1294   0.0
AFK893 (AFK893Q) /CSM/AF/AFK8-D/AFK893Q.Seq.d/                       1285   0.0
SFJ265 (SFJ265Q) /CSM/SF/SFJ2-C/SFJ265Q.Seq.d/                       1281   0.0
SFG772 (SFG772Q) /CSM/SF/SFG7-C/SFG772Q.Seq.d/                       1281   0.0
SFF891 (SFF891Q) /CSM/SF/SFF8-D/SFF891Q.Seq.d/                       1281   0.0
SFF850 (SFF850Q) /CSM/SF/SFF8-C/SFF850Q.Seq.d/                       1281   0.0
SFJ287 (SFJ287Q) /CSM/SF/SFJ2-D/SFJ287Q.Seq.d/                       1273   0.0
CFJ329 (CFJ329Q) /CSM/CF/CFJ3-B/CFJ329Q.Seq.d/                       1269   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U61987|U61987.1 Dictyostelium discoideum phosphoprotein phosphatase A (pppA) mRNA, complete cds.  1140   0.0    4
AL428358|AL428358.1 clone BA0AB025C09 of library BA0AB from strain CLIB 210 of  Kluyveromyces lactis.    48   2e-11  3
BU276111|BU276111.1 Cr_Emb_06C12_TEXF1 Convoluta roscoffensis embryos from Eva Jiminez Convoluta roscoffensis cDNA clone Cr_Emb_06C12 5' similar to Q23922 PHOSPHOPROTEIN PHOSPHATASE A. Dictyostelium discoideum, mRNA sequence.    58   2e-09  4
BI516804|BI516804.1 BB160023B10E05 Bee Brain Normalized Library, BB16 Apis mellifera cDNA clone BB160023B10E05 5', mRNA sequence.    44   9e-07  3
AX829534|AX829534.1 Sequence 254 from Patent WO03072602.           44   3e-05  3
AX818504|AX818504.1 Sequence 254 from Patent EP1338608.            44   3e-05  3
AX596522|AX596522.1 Sequence 2176 from Patent EP1258494.           44   3e-05  3
L05146|L05146.3 Saccharomyces cerevisiae chromosome I left arm sequence.    44   8e-05  4
M98389|M98389.1 Yeast protein phosphatase regulatory subunit...    44   1e-04  3
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    34   5e-04  20

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U61987_1(U61987|pid:none) Dictyostelium discoideum phosphoprotei...   377   e-103
AM920433_216(AM920433|pid:none) Penicillium chrysogenum Wisconsi...   273   1e-71
AM270402_10(AM270402|pid:none) Aspergillus niger contig An18c010...   268   3e-70
(Q9UT08) RecName: Full=Protein phosphatase PP2A regulatory subun...   262   2e-68
AJ131045_1(AJ131045|pid:none) Cicer arietinum mRNA for protein p...   261   4e-68
AF196287_1(AF196287|pid:none) Medicago sativa subsp. x varia Ser...   258   3e-67
BT085865_1(BT085865|pid:none) Zea mays full-length cDNA clone ZM...   257   5e-67
AJ243828_1(AJ243828|pid:none) Oryza sativa mRNA for protein phos...   257   7e-67
BC027596_1(BC027596|pid:none) Homo sapiens protein phosphatase 2...   250   1e-66
AY892682_1(AY892682|pid:none) Synthetic construct Homo sapiens c...   249   1e-66

 psg: 0.75  gvh: 0.39  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 16.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VFJ649 is cyt

>VFJ649F.Seq
ATATATAAAAAATGGCATCTATAAATACAGAATCGGATGATTATNTCCAATTGTTATTTT
AATTGATGAATTAAAAAATGAGGATATACAACTTAGACTTAATTCAATAAAAAAACTTCA
ATCAATTGCAAAAGCATTAGGACCAGAACGTACCCGTACAGAATTAATCCCATATTTACA
AGACTCTGTTCTCGAAGATGAAGATGAAGTTTTAGTTGTATTATCAGAAGAATTAGGAAA
TTTAATCGAGTTTGTTGGTGGTGCAGAACATGCTGTTTGTTTATTACCACCATTACAAAT
TTTAGCAGGTGCTGAGGAATTAGTTGTTAGAGAAAAGGCAGTGGAATCATTATGTAAAAT
TGCAAAAGAGATCCCAACATCATCATTCGAAGAATCATTCCTTCCATTATTATTTTCATT
ATCAAAAGCAGATTGGTTTACATCACGTACATCAGCATGTGGACTTTTCACTGTATCATA
TCCAAGAGCAAATGCTGAAATGAAGAAATCTTTAAGAAAAACTTTTGGGGGATTATATCA
CGATGATACACCAATGNNNNNNNNNN

>VFJ649Z.Seq
NNNNNNNNNNGAGCTGCACTTGGCTCCAAGTCATTATGTCATTGGCACCCAATTTATGGT
AAAGAAGATACATTAACTCATCTTTTAGAATTGTTTTTTACATCTCTTAAAAGATGATTT
CCCAGATGTTCGTTTAAATATCATTTCAAAATTAGATCAAGTAAGTAAAGTAATTGGTAT
TGAAATGTTATCACAATCTCTATTACCAGCTATCGTTGAATTGGCTGAAGATCATCAATG
GAGAGTAAGATTAGCAATTATTGATTATATTCCACTTTTAGCTTCACAATTAGGTGTTGA
ATTCTTTGATGAAAAACTTGGTAATTTATGTATGACTTGGTTAGGTGATCCAGTATTTTC
AATTCGTGAAGCAGCAACTAATAATTTGAAGAAATTAACCGAAGTATTTGGAGTTGATTG
GGCAAAGAATAATATTATTCCAAAAGTTTTATCTTTACACTCTCATCCAAACTATCTCTA
TCGTATGACCACATTATTTTCAATTTCAACCCTTTCAACTGTTGTTGGAGGTGATGTTAT
CTCTAGTTCAATGGTTCCTTTATTGGCCAAAATGGTTTCCGATAAAGTTCCAAATATTAG
ATTTAATGTAGCTAAAACTTTCCAAACCATTATTCCATTACTCGATAGCACAATTGTACA
ATCAAGAGTCAAACCATACTCGTAAAACTTCANAAGATACNATAAAGA

>VFJ649P.Seq
ATATATAAAAAATGGCATCTATAAATACAGAATCGGATGATTATNTCCAATTGTTATTTT
AATTGATGAATTAAAAAATGAGGATATACAACTTAGACTTAATTCAATAAAAAAACTTCA
ATCAATTGCAAAAGCATTAGGACCAGAACGTACCCGTACAGAATTAATCCCATATTTACA
AGACTCTGTTCTCGAAGATGAAGATGAAGTTTTAGTTGTATTATCAGAAGAATTAGGAAA
TTTAATCGAGTTTGTTGGTGGTGCAGAACATGCTGTTTGTTTATTACCACCATTACAAAT
TTTAGCAGGTGCTGAGGAATTAGTTGTTAGAGAAAAGGCAGTGGAATCATTATGTAAAAT
TGCAAAAGAGATCCCAACATCATCATTCGAAGAATCATTCCTTCCATTATTATTTTCATT
ATCAAAAGCAGATTGGTTTACATCACGTACATCAGCATGTGGACTTTTCACTGTATCATA
TCCAAGAGCAAATGCTGAAATGAAGAAATCTTTAAGAAAAACTTTTGGGGGATTATATCA
CGATGATACACCAATG----------GAGCTGCACTTGGCTCCAAGTCATTATGTCATTG
GCACCCAATTTATGGTAAAGAAGATACATTAACTCATCTTTTAGAATTGTTTTTTACATC
TCTTAAAAGATGATTTCCCAGATGTTCGTTTAAATATCATTTCAAAATTAGATCAAGTAA
GTAAAGTAATTGGTATTGAAATGTTATCACAATCTCTATTACCAGCTATCGTTGAATTGG
CTGAAGATCATCAATGGAGAGTAAGATTAGCAATTATTGATTATATTCCACTTTTAGCTT
CACAATTAGGTGTTGAATTCTTTGATGAAAAACTTGGTAATTTATGTATGACTTGGTTAG
GTGATCCAGTATTTTCAATTCGTGAAGCAGCAACTAATAATTTGAAGAAATTAACCGAAG
TATTTGGAGTTGATTGGGCAAAGAATAATATTATTCCAAAAGTTTTATCTTTACACTCTC
ATCCAAACTATCTCTATCGTATGACCACATTATTTTCAATTTCAACCCTTTCAACTGTTG
TTGGAGGTGATGTTATCTCTAGTTCAATGGTTCCTTTATTGGCCAAAATGGTTTCCGATA
AAGTTCCAAATATTAGATTTAATGTAGCTAAAACTTTCCAAACCATTATTCCATTACTCG
ATAGCACAATTGTACAATCAAGAGTCAAACCATACTCGTAAAACTTCANAAGATACNATA
AAGA