>VFJ446 (VFJ446Q) /CSM/VF/VFJ4-B/VFJ446Q.Seq.d/
XXXXXXXXXXAAGAACCAATCGATATCGTCAAAGGTATTCGTCCAGAACAAACTAAAAAC
CTCGCTGAGAAATTAGGTTTCACCGGTGAGAAAGCAAAGATCGCTCAACAACAAATGGAA
AACCTCTATCAATTATTCATCAAGAGTGATGCAACTCAAGTCGAGATTAACCCATTCGCT
GAAACCACCGATGGTCAAGTCATTTGTATGGATGCTAAAATCAATTTCGATGACAATGCC
TCATTCCGTCAAAAGGAGATTTTCGAAATGAGAGATACCGCCGAAGAGGATCCACGTGAG
GTTGAAGCTGGTAAATTTGGTCTCAATTACATTGGTCTCGATGGTAACATTGGTTGTATG
GTTAATGGTGCTGGTTTGGCTATGGCCACCATGGATATCATTAAATTAAAGGGTGGTATC
CCTGCTAATTTCCTCGATGTAGGTGGTAGTGCCTCTGAACAAGCCGTCACTGAAGCCTTC
AAAATCCTCACTAAAGATCCAAGAGTTAAATGTTTACTCGTAAACATTTTCGGTGGTATC
ATGAAATGTGATATCATTGCCTCTGGTATCGTAAATGCTTCAAAACAAGTTGGTCTTAAA
ATTCCATTAGTTGTACGTTTAGAAGGTACAAATGTAAACATTGGTAAAGAAATCTTGGAA
AAATCTGGTTTAAATATTACTTCTGCTAGANATTTAGATGA

---EPIDIVKGIRPEQTKNLAEKLGFTGEKAKIAQQQMENLYQLFIKSDATQVEINPFAE
TTDGQVICMDAKINFDDNASFRQKEIFEMRDTAEEDPREVEAGKFGLNYIGLDGNIGCMV
NGAGLAMATMDIIKLKGGIPANFLDVGGSASEQAVTEAFKILTKDPRVKCLLVNIFGGIM
KCDIIASGIVNASKQVGLKIPLVVRLEGTNVNIGKEILEKSGLNITSARXLD

Frame A:
---rtnryrqryssrtn*kpr*eirfhr*eskdrsttngkplsiihqe*cnssrd*pir*
nhrwsshlygc*nqfr*qclipskgdfrneryrrrgst*g*sw*iwsqlhwsrw*hwlyg
*wcwfgyghhgyh*ikgwypc*fprcrw*cl*tsrh*slqnph*rsks*mftrkhfrwyh
em*yhclwyrkcfktsws*nsisctfrrykckhw*rnlgkiwfkyyfc*xfr*


Frame B:
---EPIDIVKGIRPEQTKNLAEKLGFTGEKAKIAQQQMENLYQLFIKSDATQVEINPFAE
TTDGQVICMDAKINFDDNASFRQKEIFEMRDTAEEDPREVEAGKFGLNYIGLDGNIGCMV
NGAGLAMATMDIIKLKGGIPANFLDVGGSASEQAVTEAFKILTKDPRVKCLLVNIFGGIM
KCDIIASGIVNASKQVGLKIPLVVRLEGTNVNIGKEILEKSGLNITSARXLD


Frame C:
---nqsisskvfvqnklktslrn*vspvrkqrslnnkwktsinyssrvmqlksrlthslk
ppmvksfvwmlksismtmphsvkrrfsk*eippkrihvrlklvnlvsitlvsmvtlvvwl
mvlvwlwppwisln*rvvsllissm*vvvplnkpslkpsksslkiqelnvys*tfsvvs*
nvislplvs*mlqnklvlkfh*lyv*kvqm*tlvkkswknlv*illllxi*m

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFJ446 (VFJ446Q) /CSM/VF/VFJ4-B/VFJ446Q.Seq.d/                       1364   0.0
VFC521 (VFC521Q) /CSM/VF/VFC5-A/VFC521Q.Seq.d/                       1356   0.0
SFK777 (SFK777Q) /CSM/SF/SFK7-D/SFK777Q.Seq.d/                       1348   0.0
SFH344 (SFH344Q) /CSM/SF/SFH3-B/SFH344Q.Seq.d/                       1348   0.0
SFC186 (SFC186Q) /CSM/SF/SFC1-D/SFC186Q.Seq.d/                       1348   0.0
AFG227 (AFG227Q) /CSM/AF/AFG2-B/AFG227Q.Seq.d/                       1348   0.0
AFI855 (AFI855Q) /CSM/AF/AFI8-C/AFI855Q.Seq.d/                       1324   0.0
AFF274 (AFF274Q) /CSM/AF/AFF2-D/AFF274Q.Seq.d/                       1320   0.0
VFF668 (VFF668Q) /CSM/VF/VFF6-C/VFF668Q.Seq.d/                       1314   0.0
VFD829 (VFD829Q) /CSM/VF/VFD8-B/VFD829Q.Seq.d/                       1314   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.  1356   0.0    1
AC115594|AC115594.2 Dictyostelium discoideum chromosome 2 map 4071862-4101005 strain AX4, complete sequence.    62   3e-29  8
X84222|X84222.2 Neocallimastix frontalis mRNA for beta-succinyl CoA synthetase precursor.    74   3e-21  4
AF493055|AF493055.1 Neocallimastix patriciarum succinyl-CoA synthetase, beta subunit (scsB) mRNA, complete cds.    74   3e-21  4
AX621832|AX621832.1 Sequence 4795 from Patent WO02094868.          52   3e-19  4
AR354123|AR354123.1 Sequence 241 from patent US 6593114.           52   2e-17  4
BJ519880|BJ519880.1 Oryzias latipes cDNA clone:MF01SSB014A03, 5' end single read.    70   2e-16  2
BJ532800|BJ532800.1 Oryzias latipes cDNA clone:MF01SSB015A03, 3' end single read.    70   2e-16  2
BJ520215|BJ520215.1 Oryzias latipes cDNA clone:MF01SSB015A03, 5' end single read.    70   2e-16  2
CC820121|CC820121.1 1O0001G05R Oxytricha plasmid UUGC1O library Sterkiella histriomuscorum genomic clone UUGC1O0001G05 R, genomic survey sequence.    84   1e-15  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC115594_9(AC115594|pid:none) Dictyostelium discoideum chromosom...   308   1e-82
(Q55AI5) RecName: Full=Succinyl-CoA ligase [ADP-forming] subunit...   308   1e-82
BT039713_1(BT039713|pid:none) Zea mays full-length cDNA clone ZM...   301   2e-80
(Q6K9N6) RecName: Full=Succinyl-CoA ligase [GDP-forming] subunit...   300   2e-80
EF530042_1(EF530042|pid:none) Caragana jubata succinyl-CoA ligas...   298   1e-79
(O82662) RecName: Full=Succinyl-CoA ligase [GDP-forming] subunit...   293   3e-78
AY087596_1(AY087596|pid:none) Arabidopsis thaliana clone 36904 m...   291   1e-77
DQ200398_1(DQ200398|pid:none) Solanum tuberosum clone 071C05 suc...   290   3e-77
DQ294279_1(DQ294279|pid:none) Solanum tuberosum clone 180E05 suc...   290   3e-77
BC084473_1(BC084473|pid:none) Xenopus tropicalis hypothetical LO...   286   5e-76

 psg: 0.54  gvh: 0.34  alm: 0.33  top: 0.53  tms: 0.00  mit: 0.11  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VFJ446 is cyt

>VFJ446Z.Seq
NNNNNNNNNNAAGAACCAATCGATATCGTCAAAGGTATTCGTCCAGAACAAACTAAAAAC
CTCGCTGAGAAATTAGGTTTCACCGGTGAGAAAGCAAAGATCGCTCAACAACAAATGGAA
AACCTCTATCAATTATTCATCAAGAGTGATGCAACTCAAGTCGAGATTAACCCATTCGCT
GAAACCACCGATGGTCAAGTCATTTGTATGGATGCTAAAATCAATTTCGATGACAATGCC
TCATTCCGTCAAAAGGAGATTTTCGAAATGAGAGATACCGCCGAAGAGGATCCACGTGAG
GTTGAAGCTGGTAAATTTGGTCTCAATTACATTGGTCTCGATGGTAACATTGGTTGTATG
GTTAATGGTGCTGGTTTGGCTATGGCCACCATGGATATCATTAAATTAAAGGGTGGTATC
CCTGCTAATTTCCTCGATGTAGGTGGTAGTGCCTCTGAACAAGCCGTCACTGAAGCCTTC
AAAATCCTCACTAAAGATCCAAGAGTTAAATGTTTACTCGTAAACATTTTCGGTGGTATC
ATGAAATGTGATATCATTGCCTCTGGTATCGTAAATGCTTCAAAACAAGTTGGTCTTAAA
ATTCCATTAGTTGTACGTTTAGAAGGTACAAATGTAAACATTGGTAAAGAAATCTTGGAA
AAATCTGGTTTAAATATTACTTCTGCTAGANATTTAGATGA