>VFJ441 (VFJ441Q) /CSM/VF/VFJ4-B/VFJ441Q.Seq.d/
CACTGTTGGCCTACTGGTTTTTAGCTGCTATTGTTTTAGCTAAGCTTTAACAGTACCATT
AAACTTCCATCAAGCTTCAAGAGAATCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAG
ATTATCTGCTCTCAATGCTGGTACCACAATCCCAATTTCAGATTTTGAAGATGCTCAATA
CTATGGTGCCATTACCATTGGTACCCCAGGTCAAGCCTTCAAAGTAGTTNTCGATACTGG
TTCATCCAACTTGTGGATTCCATCAAAGAAATGTCCAATCACTGTTGTTGCATGTGATTT
ACATAACAAATATAACAGCGGTGCCTCAAGCACATATGTCGCCAACGGAACTGATTTCAC
CATCCAATACGGTAGTGGXXXXXXXXXXGTATTTTAGGTCTTGCTTTCCAATCCATCTCT
GTTAATTCAATTCCACCAGTCTTTTACAACATGTTATCACAAGGTTTAGTTTCATCAACA
CTCTTCTCCTTCTGGTTATCAAGAACTCCAGGTGCCAACGGTGGTGAACTCTCATTCGGT
TCAATCGATAACACCAAATACACTGGTGACATTACCTACGTCCCATTAACCAACGAAACC
TATTGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGT
ACTACTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGGTCCAATGGCTGAT
ATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCT
GATTGTAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTT
GTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTTG
AGTGGATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATCCTTGGTGATGTTTTC
ATCTCTGCTTACTATACTGTATTCGATTTTGGTAATAAACAAGTTGGTTTCGCAACTGCC
ATTCAAGGTTAAATTTTTTTAATTAATTTATATTTAAGATAGAAATAAAACTAAATAATA
GA

tvgllvfscycfs*ALTVPLNFHQASRESRRRVPQKWSNRLSALNAGTTIPISDFEDAQY
YGAITIGTPGQAFKVVXDTGSSNLWIPSKKCPITVVACDLHNKYNSGASSTYVANGTDFT
IQYGS---

---ILGLAFQSISVNSIPPVFYNMLSQGLVSSTLFSFWLSRTPGANGGELSFGSIDNTKY
TGDITYVPLTNETYWEFVMDDFAIDGQSAGFCGTTCHAICDSGTSLIAGPMADITALNEK
LGAVILNGEGVFSDCSVINTLPNVTITVAGREFVLTPKEYVLEVTEFGKTECLSGFMGIE
LNMGNFWILGDVFISAYYTVFDFGNKQVGFATAIQG*iflinlylr*k*n*ii

Frame A:
hcwptgf*lllf*lsfnstiklpssfkri*kksstkmvkqiicsqcwyhnpnfrf*rcsi
lwchyhwyprsslqssxrywfiqlvdsikemsnhcccm*ft*qi*qrclkhicrqrn*fh
hpir*w---

---vf*vllsnpslliqfhqsfttcyhkv*fhqhsspsgyqelqvptvvnshsvqsitpn
tlvtlptsh*ptkpignslwmtllsmvnqlvsvvllvtqfaiqvhhsllvqwlillpsmk
n*vlss*mvkvsslivalstpyqmlpspllvvnlf*lqkntf*kllsserlnv*vdlwvs
s*tweisgslvmfsslltilysilvinklvsqlpfkvkff*liyi*drnktk**

Frame B:
tvgllvfscycfs*ALTVPLNFHQASRESRRRVPQKWSNRLSALNAGTTIPISDFEDAQY
YGAITIGTPGQAFKVVXDTGSSNLWIPSKKCPITVVACDLHNKYNSGASSTYVANGTDFT
IQYGS---

---yfrscfpihlc*fnstsllqhvitrfsfintllllviknsrcqrw*tlirfnr*hqi
hw*hylrpinqrnllgiryg*lcyrwsiswflwyylsrnlrfryithcwsng*yycpq*k
ircchlkw*rcll*l*ryqhltkcyhhrcws*icfnskrirfrsy*vrkd*mfewiygyr
vkhgkfldpw*cfhlcllycirfw**tswfrnchsrlnffn*fifkieiklnnr

Frame C:
llaywflaaivlakl*qyh*tsiklqenleeefhkngqtdyllsmlvpqsqfqilkmlnt
mvplplvpqvkpsk*xsilvhptcgfhqrnvqslllhviyitnitavpqahmsptelisp
sntvv---

---ILGLAFQSISVNSIPPVFYNMLSQGLVSSTLFSFWLSRTPGANGGELSFGSIDNTKY
TGDITYVPLTNETYWEFVMDDFAIDGQSAGFCGTTCHAICDSGTSLIAGPMADITALNEK
LGAVILNGEGVFSDCSVINTLPNVTITVAGREFVLTPKEYVLEVTEFGKTECLSGFMGIE
LNMGNFWILGDVFISAYYTVFDFGNKQVGFATAIQG*iflinlylr*k*n*ii

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFJ441 (VFJ441Q) /CSM/VF/VFJ4-B/VFJ441Q.Seq.d/                       2038   0.0
VFM519 (VFM519Q) /CSM/VF/VFM5-A/VFM519Q.Seq.d/                       1334   0.0
VFL883 (VFL883Q) /CSM/VF/VFL8-D/VFL883Q.Seq.d/                       1334   0.0
VFL410 (VFL410Q) /CSM/VF/VFL4-A/VFL410Q.Seq.d/                       1334   0.0
VFL274 (VFL274Q) /CSM/VF/VFL2-D/VFL274Q.Seq.d/                       1334   0.0
VFL252 (VFL252Q) /CSM/VF/VFL2-C/VFL252Q.Seq.d/                       1334   0.0
VFI870 (VFI870Q) /CSM/VF/VFI8-C/VFI870Q.Seq.d/                       1334   0.0
VFG845 (VFG845Q) /CSM/VF/VFG8-B/VFG845Q.Seq.d/                       1334   0.0
VFG815 (VFG815Q) /CSM/VF/VFG8-A/VFG815Q.Seq.d/                       1334   0.0
VFF721 (VFF721Q) /CSM/VF/VFF7-A/VFF721Q.Seq.d/                       1334   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

Y16962|Y16962.1 Dictyostelium discoideum mRNA for cathepsin D.   1279   0.0    2
AJ243946|AJ243946.1 Dictyostelium discoideum ctsD gene for cathepsin D, exons 1 to 2.  1279   0.0    2
AF076243|AF076243.1 Arabidopsis thaliana BAC T26N6 from chromosome IV at 19.3 cM, complete sequence.    62   7e-06  4
AL161500|AL161500.2 Arabidopsis thaliana DNA chromosome 4, contig fragment No. 12.    62   2e-05  1
CD825453|CD825453.1 BN25.060N03F011129 BN25 Brassica napus cDNA clone BN25060N03, mRNA sequence.    62   2e-05  1
BX838383|BX838383.1 Arabidopsis thaliana Full-length cDNA 5PRIM end of clone  GSLTSIL89ZD03 of Silique of strain col-0 of Arabidopsis thaliana  (thale cress).    62   2e-05  1
BX827174|BX827174.1 Arabidopsis thaliana Full-length cDNA Complete sequence from clone  GSLTLS1ZB10 of Adult vegetative tissue of strain col-0 of  Arabidopsis thaliana (thale cress).    62   2e-05  1
BX829292|BX829292.1 Arabidopsis thaliana Full-length cDNA Complete sequence from clone  GSLTSIL89ZE03 of Silique of strain col-0 of Arabidopsis thaliana  (thale cress).    62   2e-05  1
AV567523|AV567523.1 Arabidopsis thaliana cDNA clone:SQL15g03F, 3' end.    62   2e-05  1
CB264640|CB264640.1 48-E014661-035-002-P12-T7R MPIZ-ADIS-035 Arabidopsis thaliana cDNA clone MPIZp2000P122Q 5-PRIME, mRNA sequence.    62   2e-05  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BT080419_1(BT080419|pid:none) Caligus clemensi clone ccle-evs-50...   220   6e-56
AB440200_1(AB440200|pid:none) Thunnus orientalis PGA mRNA for pe...   155   1e-55
AF156787_1(AF156787|pid:none) Pleuronectes americanus pepsinogen...   151   1e-55
EU259064_1(EU259064|pid:none) Epinephelus coioides pepsinogen A2...   157   2e-55
(Q03168) RecName: Full=Lysosomal aspartic protease;          EC=...   219   2e-55
AF156788_1(AF156788|pid:none) Pleuronectes americanus pepsinogen...   163   3e-55
EU807926_1(EU807926|pid:none) Siniperca scherzeri pepsinogen A1 ...   150   3e-55
EU163285_1(EU163285|pid:none) Diplodus sargus pepsinogen mRNA, c...   162   6e-55
EU807925_1(EU807925|pid:none) Siniperca chuatsi pepsinogen A1 pr...   149   1e-54
AY167037_1(AY167037|pid:none) Danio rerio renin precursor, mRNA,...   144   2e-54

 psg: 0.85  gvh: 0.36  alm: 0.41  top: 0.77  tms: 0.00  mit: 0.42  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 28.0 %: mitochondrial
	 28.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VFJ441 is nuc

>VFJ441F.Seq
CACTGTTGGCCTACTGGTTTTTAGCTGCTATTGTTTTAGCTAAGCTTTAACAGTACCATT
AAACTTCCATCAAGCTTCAAGAGAATCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAG
ATTATCTGCTCTCAATGCTGGTACCACAATCCCAATTTCAGATTTTGAAGATGCTCAATA
CTATGGTGCCATTACCATTGGTACCCCAGGTCAAGCCTTCAAAGTAGTTNTCGATACTGG
TTCATCCAACTTGTGGATTCCATCAAAGAAATGTCCAATCACTGTTGTTGCATGTGATTT
ACATAACAAATATAACAGCGGTGCCTCAAGCACATATGTCGCCAACGGAACTGATTTCAC
CATCCAATACGGTAGTGGNNNNNNNNNN

>VFJ441Z.Seq
NNNNNNNNNNGTATTTTAGGTCTTGCTTTCCAATCCATCTCTGTTAATTCAATTCCACCA
GTCTTTTACAACATGTTATCACAAGGTTTAGTTTCATCAACACTCTTCTCCTTCTGGTTA
TCAAGAACTCCAGGTGCCAACGGTGGTGAACTCTCATTCGGTTCAATCGATAACACCAAA
TACACTGGTGACATTACCTACGTCCCATTAACCAACGAAACCTATTGGGAATTCGTTATG
GATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATT
TGCGATTCAGGTACATCACTCATTGCTGGTCCAATGGCTGATATTACTGCCCTCAATGAA
AAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAAC
ACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGAA
TACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTTGAGTGGATTTATGGGTATC
GAGTTAAACATGGGAAATTTCTGGATCCTTGGTGATGTTTTCATCTCTGCTTACTATACT
GTATTCGATTTTGGTAATAAACAAGTTGGTTTCGCAACTGCCATTCAAGGTTAAATTTTT
TTAATTAATTTATATTTAAGATAGAAATAAAACTAAATAATAGA

>VFJ441P.Seq
CACTGTTGGCCTACTGGTTTTTAGCTGCTATTGTTTTAGCTAAGCTTTAACAGTACCATT
AAACTTCCATCAAGCTTCAAGAGAATCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAG
ATTATCTGCTCTCAATGCTGGTACCACAATCCCAATTTCAGATTTTGAAGATGCTCAATA
CTATGGTGCCATTACCATTGGTACCCCAGGTCAAGCCTTCAAAGTAGTTNTCGATACTGG
TTCATCCAACTTGTGGATTCCATCAAAGAAATGTCCAATCACTGTTGTTGCATGTGATTT
ACATAACAAATATAACAGCGGTGCCTCAAGCACATATGTCGCCAACGGAACTGATTTCAC
CATCCAATACGGTAGTGG----------GTATTTTAGGTCTTGCTTTCCAATCCATCTCT
GTTAATTCAATTCCACCAGTCTTTTACAACATGTTATCACAAGGTTTAGTTTCATCAACA
CTCTTCTCCTTCTGGTTATCAAGAACTCCAGGTGCCAACGGTGGTGAACTCTCATTCGGT
TCAATCGATAACACCAAATACACTGGTGACATTACCTACGTCCCATTAACCAACGAAACC
TATTGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGT
ACTACTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGGTCCAATGGCTGAT
ATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCT
GATTGTAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTT
GTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTTG
AGTGGATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATCCTTGGTGATGTTTTC
ATCTCTGCTTACTATACTGTATTCGATTTTGGTAATAAACAAGTTGGTTTCGCAACTGCC
ATTCAAGGTTAAATTTTTTTAATTAATTTATATTTAAGATAGAAATAAAACTAAATAATA
GA