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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFJ403
Library VF
(Link to library)
Clone ID VFJ403
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VFJ403P
(Link to Original site)
Representative DNA sequence 
>VFJ403 (VFJ403Q) /CSM/VF/VFJ4-A/VFJ403Q.Seq.d/
AGTTNAAGTGATATGAAACATTGGCCGTTCAAAGTTATTCCAAAGGATGGTGATAAGCCA
CATATTCAAGTTGAATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCA
ATGGTACTCTTAAAAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAAT
GCTGTAATTACCGTTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCT
GGTACAATTTCAAAATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATT
GCTTATGGTTTAGAAAAGAAAGGTTCTGGTGAAAAGAATATTTTAATTTTCGATTTAGGT
GGTGGTACATTTGATGTTTCTCTCCTTACCATTGAAGATGGTGTATTTGAAGTTAAAGCC
ACCGCTGGTGATACTCATTTAGGTGGTGAAGATTTTGATAATCGTCTCGTTTCTCACTTT
GTTGATGAATTTAAACGTAAACATAAAAAAGATATTATGGGTAATCAACGTGXXXXXXXX
XXACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAAT
TTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTT
GAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCA
ACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAA
ATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGAT
CGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAA
GATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACT
GAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGAT
AAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTATCAAGAAGGA
GGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACCAAAGAGTTCA
AACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACATCTCTATCAAA
TAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAA
sequence update 2001.11.22
Translated Amino Acid sequence 
SXSDMKHWPFKVIPKDGDKPHIQVEFKGETKVFSPEEISSMVLLKMKETAEAYLGKTINN
AVITVPAYFNDSQRQATKDAGTISKLNVQRIINEPTAAAIAYGLEKKGSGEKNILIFDLG
GGTFDVSLLTIEDGVFEVKATAGDTHLGGEDFDNRLVSHFVDEFKRKHKKDIMGNQR---

---PGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNVSAED
KSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFTVKNS
IKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMSKLYQ
EGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*kiis**nq
Translated Amino Acid sequence (All Frames) 
Frame A:
SXSDMKHWPFKVIPKDGDKPHIQVEFKGETKVFSPEEISSMVLLKMKETAEAYLGKTINN
AVITVPAYFNDSQRQATKDAGTISKLNVQRIINEPTAAAIAYGLEKKGSGEKNILIFDLG
GGTFDVSLLTIEDGVFEVKATAGDTHLGGEDFDNRLVSHFVDEFKRKHKKDIMGNQR---

---trcinssl*r*tcydqr**fig*i*tfwyststkrctts*syi*c*ckwylkcis*r
*inw*qtkdynyq**rssfkrrn*kngc*creiqttr*ttkrsc*ike*igklcihc*kf
n*r*kscsqnfrfr*iny*i*n*kctqmvriksnrrkg*i*r*deslrsscqsnhe*tls
rrryatrwwyarwyvk*ftkefkqqsrrirlteffrgkhlyqidflknnklvks

Frame B:
vxvi*nigrsklfqrmvishifklnskvklkfshqkkslqwys*k*kkplklilvkplim
l*lpfqlismivnvkqpkmlvqfqn*mfnvllmnqllllllmv*krkvlvkrif*fsi*v
vvhlmflslplkmvylklkpplvili*vvkiliivsfltllmnlnvnikkilwvinv---

---PGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNVSAED
KSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFTVKNS
IKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMSKLYQ
EGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*kiis**nq

Frame C:
xk*yetlavqsyskgw**atyss*iqr*n*sfltrrnlfngtlknernr*slsw*nh**c
cnyrsslfq**stssnqrcwynfkikcstyy**tncccyclwfrkerfw*keyfnfrfrw
wyi*cfspyh*rwci*s*shrw*ysfrw*rf**ssrfslc**i*t*t*kryyg*st---

---qvy*fkfmkvnvl*pkiiiywvnlnflvfhqhqevyhklklhlmlmqmvs*myqlki
nqlvtnkrlqlpmikvvfqkkklkkwllmqrnsnnkmnnkkivlnqrinwkimhsllkiq
lkmkklqpkfqiqinqllnlklkvysng*nqikpqkrmnmkir*kp*kqlsiqs*vnsik
kevchkvvvcqvvcqmihqrvqttk*tn*idgifqrktslsnrflkk**vski
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ260 (VHQ260Q) /CSM/VH/VHQ2-C/VHQ260Q.Seq.d/                       1376   0.0
VHQ240 (VHQ240Q) /CSM/VH/VHQ2-B/VHQ240Q.Seq.d/                       1376   0.0
VHP873 (VHP873Q) /CSM/VH/VHP8-D/VHP873Q.Seq.d/                       1376   0.0
VHP719 (VHP719Q) /CSM/VH/VHP7-A/VHP719Q.Seq.d/                       1376   0.0
VHP716 (VHP716Q) /CSM/VH/VHP7-A/VHP716Q.Seq.d/                       1376   0.0
VHP437 (VHP437Q) /CSM/VH/VHP4-B/VHP437Q.Seq.d/                       1376   0.0
VHP420 (VHP420Q) /CSM/VH/VHP4-A/VHP420Q.Seq.d/                       1376   0.0
VHP266 (VHP266Q) /CSM/VH/VHP2-C/VHP266Q.Seq.d/                       1376   0.0
VHP236 (VHP236Q) /CSM/VH/VHP2-B/VHP236Q.Seq.d/                       1376   0.0
VHP232 (VHP232Q) /CSM/VH/VHP2-B/VHP232Q.Seq.d/                       1376   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).  1376   0.0    1
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.  1368   0.0    1
AF025951|AF025951.1 Dictyostelium discoideum heat-shock cognate protein 70 (hsc70) mRNA, complete cds.   285   e-155  7
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.   285   e-150  11
A58313|A58313.1 Sequence 1 from Patent WO9636707.                 125   e-118  9
Z30210|Z30210.1 C.albicans (ATCC 20955) cahsp70 mRNA for heat shock protein 70.   125   e-118  9
AX536934|AX536934.1 Sequence 535 from Patent WO02064766.          125   e-118  9
AJ310880|AJ310880.1 Cryptosporidium baileyi partial hsp70 gene for heat shock protein  70.   100   e-101  8
AF221539|AF221539.1 Cryptosporidium baileyi heat shock protein 70 (HSP70) gene, partial cds.   100   e-101  8
AF278536|AF278536.1 Parastrongyloides trichosuri heat shock 70 protein gene, complete cds.   137   e-100  9
dna update 2004. 2.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   352   2e-95
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   350   6e-95
(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   330   8e-89
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   328   3e-88
(Q54BE0) RecName: Full=Heat shock cognate 70 kDa protein 3;           305   2e-81
AY582833_1(AY582833|pid:none) Chytriomyces confervae strain CBS ...   304   4e-81
EU679414_1(EU679414|pid:none) Tetranychus cinnabarinus heat shoc...   301   3e-80
U71151_1(U71151|pid:none) Pneumocystis carinii heat shock protei...   301   4e-80
U80967_1(U80967|pid:none) Pneumocystis carinii f. sp. carinii he...   301   4e-80
EU679412_1(EU679412|pid:none) Tetranychus cinnabarinus heat shoc...   300   5e-80
protein update 2009. 6.22
PSORT 

 psg: 0.45  gvh: 0.33  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.07  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: nuclear
	 24.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VFJ403 is nuc
5' end seq. ID VFJ403F
5' end seq. 
>VFJ403F.Seq
AGTTNAAGTGATATGAAACATTGGCCGTTCAAAGTTATTCCAAAGGATGGTGATAAGCCA
CATATTCAAGTTGAATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCA
ATGGTACTCTTAAAAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAAT
GCTGTAATTACCGTTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCT
GGTACAATTTCAAAATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATT
GCTTATGGTTTAGAAAAGAAAGGTTCTGGTGAAAAGAATATTTTAATTTTCGATTTAGGT
GGTGGTACATTTGATGTTTCTCTCCTTACCATTGAAGATGGTGTATTTGAAGTTAAAGCC
ACCGCTGGTGATACTCATTTAGGTGGTGAAGATTTTGATAATCGTCTCGTTTCTCACTTT
GTTGATGAATTTAAACGTAAACATAAAAAAGATATTATGGGTAATCAACGTGNNNNNNNN
NN
Length of 5' end seq. 542
3' end seq. ID VFJ403Z
3' end seq. 
>VFJ403Z.Seq
NNNNNNNNNNACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAAAG
ATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGTAC
CACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAG
ATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTCAA
AAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACAAC
AAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATT
CAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAAT
CTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAAT
ATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTATC
AAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACCAA
AGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACATCT
CTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAA
Length of 3' end seq. 704
Connected seq. ID VFJ403P
Connected seq. 
>VFJ403P.Seq
AGTTNAAGTGATATGAAACATTGGCCGTTCAAAGTTATTCCAAAGGATGGTGATAAGCCA
CATATTCAAGTTGAATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCA
ATGGTACTCTTAAAAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAAT
GCTGTAATTACCGTTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCT
GGTACAATTTCAAAATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATT
GCTTATGGTTTAGAAAAGAAAGGTTCTGGTGAAAAGAATATTTTAATTTTCGATTTAGGT
GGTGGTACATTTGATGTTTCTCTCCTTACCATTGAAGATGGTGTATTTGAAGTTAAAGCC
ACCGCTGGTGATACTCATTTAGGTGGTGAAGATTTTGATAATCGTCTCGTTTCTCACTTT
GTTGATGAATTTAAACGTAAACATAAAAAAGATATTATGGGTAATCAACGTG--------
--ACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAAT
TTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTT
GAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCA
ACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAA
ATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGAT
CGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAA
GATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACT
GAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGAT
AAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTATCAAGAAGGA
GGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACCAAAGAGTTCA
AACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACATCTCTATCAAA
TAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAA
Length of connected seq. 1226
Full length Seq ID -
Full length Seq. -
Length of full length seq. -