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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFJ281
Library VF
(Link to library)
Clone ID VFJ281
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16182-1
Original site URL
Representative seq. ID VFJ281P
(Link to Original site)
Representative DNA sequence 
>VFJ281 (VFJ281Q) /CSM/VF/VFJ2-D/VFJ281Q.Seq.d/
AACCCTAATAAAAAAATAAACTCATGTACATATAAAAATTAAAATAAAAGAAAAAAAAAT
GAAAAGGACGATAATAATATTATTATTTGTATCATTATTTGTAACATTTGTTTTATCACA
AATACCAGATAATGATCCAGAATTATGTCAACAAAGAATTCAAAGAGAGGATTGTCCACA
AACACCAGTACCATGGGGAACATTCAATGACAATGACGAAATCGAAGTCTACTATATGCA
AGCACCAGTTTTCGAAGCAGTTTTTGGAAATTTCTTTGGTAAGTTGGGTGGATATCATTC
AGCAATTGGTTTCTATGACCTTACAACTGGTTTAAATTATACAGCAGAATATGATGCTTT
CTTTGAAGTAGGAAATGGTACATTACCAAATATCATAAATGTCGATGGTAAAGATGAACT
TTTATGGTGTAATGCTGGTATCCTTTGTACTGTTCCATACATTAATGAAACTTATTGGGA
TAAAAACATCTATTCAACTGCTTCAAAAACTTATATGGGAACAATTAATGGTACTCAATT
AAATCAATATATCGAATGGATGCAACAATATAATATTTCAAATCCAGTATACCAAACATG
XXXXXXXXXXTTAGAAATTAAANAAGGNNCNATTGNCCNAATCNTTGAAAGAATTTTATC
CATCCTTGGNTTAAANAAANANATTNNCGGTCNAGGNGNATATTANTTATTAGAATTNAA
TTANCCCTTTATGNATGCNAAATATGNTTATATCCCNTTNCCNGGNTGGCCNGCNAANGG
GATCGATTATGAAAANGGCNATTTTATTCNTCCCNATTAGNTTNTTTTAAAATTAGNTTG
TTTTAAATTNAA
sequence update 2001.11.22
Translated Amino Acid sequence 
tlikk*THVHIKIKIKEKKMKRTIIILLFVSLFVTFVLSQIPDNDPELCQQRIQREDCPQ
TPVPWGTFNDNDEIEVYYMQAPVFEAVFGNFFGKLGGYHSAIGFYDLTTGLNYTAEYDAF
FEVGNGTLPNIINVDGKDELLWCNAGILCTVPYINETYWDKNIYSTASKTYMGTINGTQL
NQYIEWMQQYNISNPVYQT---

---LEIKXGXIXXIXERILSILGLXKXIXGXGXYXLLEXNXPFMXAKYXYIPXPGWPAXG
IDYEXGXFIXPX*xxlklxcfkx
Translated Amino Acid sequence (All Frames) 
Frame A:
npnkkinsctykn*nkrkknekddnniiiciiicnicfitntr**srimstknskrglst
ntstmgniq*q*rnrsllyastsfrssfwkflw*vgwisfsnwfl*pynwfklysri*cf
l*srkwyitkyhkcrw*r*tfmv*cwyplycsih**nllg*khlfncfknlygnn*wysi
ksiyrmdati*yfkssipnm---

---LEIKXGXIXXIXERILSILGLXKXIXGXGXYXLLEXNXPFMXAKYXYIPXPGWPAXG
IDYEXGXFIXPX*xxlklxcfkx

Frame B:
tlikk*THVHIKIKIKEKKMKRTIIILLFVSLFVTFVLSQIPDNDPELCQQRIQREDCPQ
TPVPWGTFNDNDEIEVYYMQAPVFEAVFGNFFGKLGGYHSAIGFYDLTTGLNYTAEYDAF
FEVGNGTLPNIINVDGKDELLWCNAGILCTVPYINETYWDKNIYSTASKTYMGTINGTQL
NQYIEWMQQYNISNPVYQT---

---*klxkxxlxxslkefypslx*xxxxxvxxxixy*nxixplxmxnmxisxxxxgxxxg
simkxailfxpixxf*n*xvlnx

Frame C:
p**knklmyi*klk*kkkk*kgr**yyylyhyl*hlfyhkyqimiqnyvnkefkerivhk
hqyhgehsmtmtkskstickhqfskqfleislvswvdiiqqlvsmtlqlv*iiqqnmmls
lk*emvhyqis*msmvkmnfygvmlvsfvlfhtlmkligiktsiqllqkliweqlmvln*
inisngcnniifqiqytkh---

---rn*xrxxxpnx*knfihpwxkxxxxrsrxilxirixlxlyxcxixlypxxxxaxxxd
rl*kxxfyssxlxxfkixlf*ix
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFJ281 (VFJ281Q) /CSM/VF/VFJ2-D/VFJ281Q.Seq.d/                       1281   0.0
VHL857 (VHL857Q) /CSM/VH/VHL8-C/VHL857Q.Seq.d/                       1061   0.0
VFL220 (VFL220Q) /CSM/VF/VFL2-A/VFL220Q.Seq.d/                       1061   0.0
VFG895 (VFG895Q) /CSM/VF/VFG8-D/VFG895Q.Seq.d/                       1025   0.0
VFC480 (VFC480Q) /CSM/VF/VFC4-D/VFC480Q.Seq.d/                        991   0.0
SSA660 (SSA660Q) /CSM/SS/SSA6-C/SSA660Q.Seq.d/                        920   0.0
VFH286 (VFH286Q) /CSM/VF/VFH2-D/VFH286Q.Seq.d/                        890   0.0
SSG340 (SSG340Q) /CSM/SS/SSG3-B/SSG340Q.Seq.d/                        846   0.0
SSB231 (SSB231Q) /CSM/SS/SSB2-B/SSB231Q.Seq.d/                        821   0.0
SLE504 (SLE504Q) /CSM/SL/SLE5-A/SLE504Q.Seq.d/                        783   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116305) Dictyostelium discoideum chromosome 2 map 1005175...  1035   0.0    3
(BJ425060) Dictyostelium discoideum cDNA clone:ddv54b16, 5' ...  1059   0.0    1
(BJ415600) Dictyostelium discoideum cDNA clone:ddv23g06, 5' ...  1059   0.0    1
(BJ414588) Dictyostelium discoideum cDNA clone:ddv19a22, 5' ...  1059   0.0    1
(BJ413283) Dictyostelium discoideum cDNA clone:ddv14n24, 5' ...  1019   0.0    1
(BJ411824) Dictyostelium discoideum cDNA clone:ddv5p20, 5' e...   839   0.0    3
(BJ389934) Dictyostelium discoideum cDNA clone:dds20i16, 5' ...   769   0.0    2
(BJ417874) Dictyostelium discoideum cDNA clone:ddv15k22, 5' ...   932   0.0    1
(AU076369) Dictyostelium discoideum slug cDNA, clone SSA660.      920   0.0    1
(AU061512) Dictyostelium discoideum slug cDNA, clone SLE504.      781   0.0    2
dna update 2009. 1.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q553W9) RecName: Full=Ceroid-lipofuscinosis neuronal protein 5 ...   348   1e-94
AC116305_28(AC116305|pid:none) Dictyostelium discoideum chromoso...   340   3e-92
AK032293_1(AK032293|pid:none) Mus musculus adult male olfactory ...    94   7e-18
AF068227_1(AF068227|pid:none) Homo sapiens putative transmembran...    94   7e-18
BC025487_1(BC025487|pid:none) Mus musculus ceroid-lipofuscinosis...    94   7e-18
AK075109_1(AK075109|pid:none) Homo sapiens cDNA FLJ90628 fis, cl...    92   3e-17
AY885123_1(AY885123|pid:none) Canis familiaris CLN5 (CLN5) gene,...    90   1e-16
(Q5JZQ9) Ceroid-lipofuscinosis neuronal protein 5 precursor (Pro...    90   1e-16
(Q1ZYR0) RecName: Full=Ceroid-lipofuscinosis neuronal protein 5;...    89   1e-16
(A2TJ54) RecName: Full=Ceroid-lipofuscinosis neuronal protein 5;...    89   2e-16
protein update 2009. 6.22
PSORT 

 psg: 0.47  gvh: 0.86  alm: 0.17  top: -0.13  tms: 0.07  mit: 0.25  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 20.0 %: nuclear
	 16.0 %: mitochondrial
	 16.0 %: Golgi
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for VFJ281 is cyt
5' end seq. ID VFJ281F
5' end seq. 
>VFJ281F.Seq
AACCCTAATAAAAAAATAAACTCATGTACATATAAAAATTAAAATAAAAGAAAAAAAAAT
GAAAAGGACGATAATAATATTATTATTTGTATCATTATTTGTAACATTTGTTTTATCACA
AATACCAGATAATGATCCAGAATTATGTCAACAAAGAATTCAAAGAGAGGATTGTCCACA
AACACCAGTACCATGGGGAACATTCAATGACAATGACGAAATCGAAGTCTACTATATGCA
AGCACCAGTTTTCGAAGCAGTTTTTGGAAATTTCTTTGGTAAGTTGGGTGGATATCATTC
AGCAATTGGTTTCTATGACCTTACAACTGGTTTAAATTATACAGCAGAATATGATGCTTT
CTTTGAAGTAGGAAATGGTACATTACCAAATATCATAAATGTCGATGGTAAAGATGAACT
TTTATGGTGTAATGCTGGTATCCTTTGTACTGTTCCATACATTAATGAAACTTATTGGGA
TAAAAACATCTATTCAACTGCTTCAAAAACTTATATGGGAACAATTAATGGTACTCAATT
AAATCAATATATCGAATGGATGCAACAATATAATATTTCAAATCCAGTATACCAAACATG
NNNNNNNNNN
Length of 5' end seq. 610
3' end seq. ID VFJ281Z
3' end seq. 
>VFJ281Z.Seq
NNNNNNNNNNTTAGAAATTAAANAAGGNNCNATTGNCCNAATCNTTGAAAGAATTTTATC
CATCCTTGGNTTAAANAAANANATTNNCGGTCNAGGNGNATATTANTTATTAGAATTNAA
TTANCCCTTTATGNATGCNAAATATGNTTATATCCCNTTNCCNGGNTGGCCNGCNAANGG
GATCGATTATGAAAANGGCNATTTTATTCNTCCCNATTAGNTTNTTTTAAAATTAGNTTG
TTTTAAATTNAA
Length of 3' end seq. 252
Connected seq. ID VFJ281P
Connected seq. 
>VFJ281P.Seq
AACCCTAATAAAAAAATAAACTCATGTACATATAAAAATTAAAATAAAAGAAAAAAAAAT
GAAAAGGACGATAATAATATTATTATTTGTATCATTATTTGTAACATTTGTTTTATCACA
AATACCAGATAATGATCCAGAATTATGTCAACAAAGAATTCAAAGAGAGGATTGTCCACA
AACACCAGTACCATGGGGAACATTCAATGACAATGACGAAATCGAAGTCTACTATATGCA
AGCACCAGTTTTCGAAGCAGTTTTTGGAAATTTCTTTGGTAAGTTGGGTGGATATCATTC
AGCAATTGGTTTCTATGACCTTACAACTGGTTTAAATTATACAGCAGAATATGATGCTTT
CTTTGAAGTAGGAAATGGTACATTACCAAATATCATAAATGTCGATGGTAAAGATGAACT
TTTATGGTGTAATGCTGGTATCCTTTGTACTGTTCCATACATTAATGAAACTTATTGGGA
TAAAAACATCTATTCAACTGCTTCAAAAACTTATATGGGAACAATTAATGGTACTCAATT
AAATCAATATATCGAATGGATGCAACAATATAATATTTCAAATCCAGTATACCAAACATG
----------TTAGAAATTAAANAAGGNNCNATTGNCCNAATCNTTGAAAGAATTTTATC
CATCCTTGGNTTAAANAAANANATTNNCGGTCNAGGNGNATATTANTTATTAGAATTNAA
TTANCCCTTTATGNATGCNAAATATGNTTATATCCCNTTNCCNGGNTGGCCNGCNAANGG
GATCGATTATGAAAANGGCNATTTTATTCNTCCCNATTAGNTTNTTTTAAAATTAGNTTG
TTTTAAATTNAA
Length of connected seq. 842
Full length Seq ID -
Full length Seq. -
Length of full length seq. -