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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFJ203
Library VF
(Link to library)
Clone ID VFJ203
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U09949-1
Original site URL
Representative seq. ID VFJ203P
(Link to Original site)
Representative DNA sequence 
>VFJ203 (VFJ203Q) /CSM/VF/VFJ2-A/VFJ203Q.Seq.d/
AATTTACTTTTAGTTATATCCTTAAAGATGTTATCAAGAAATTTAAAAACTTTAGTTAAA
AATAAAAATATATTATATTTTACTACATCAACTAGTAAATCAAATAAAATTTATGAAAAT
GCAAAGGAAGCAGTTAAAGATATTCCAAATGGTTCAAAATTATTAGTAGGTGGATTTGGA
CTTTGTGGTATTCCAGAGAATTTAATATCAGCAGTACGTGATACAGGTGTAAAGGATTTA
ACAGTTGTTAGTAATAATGCAGGTGTTGATGATTTTGGATTAGGTGTTTTATTAAAGAGT
AGACAAATTAAAAGAATGATCTCATCATATGTTGGTGAGAATGCAACCTTTGAGAGTCAA
TATCTCAAGGGTGAGCTTGAAGTAGAGTTAACACCACAAGGTAATTTAGCAGAGAGATTA
AGAGCAGGTGGTGCAGGTATTCCAGCTTTCTATACAAGTACAGGTGTTGGTACAGTGTTG
GTGGAAGAAGGTGGTTTCCCAATCAAGTATGCAAATGATGGTTCXXXXXXXXXXGAAGCA
GCTGCCAAACCAAAGGATGAGGCAGCAGCCAAGAGAGAGCGTATCGTACGTCGTGCAGCT
TTAGAGTTTGAAGATGGTATGTATTGTAATCTTGGTATTGGTATGCCAACATTGGCCAGT
AATTTCATTCCAAAGGGTATTAGAATCGAATTACAATCAGAGAATGGCTTATTAGGTATG
GGTCCATTCCCAAAAATGGGCGAACATGATGCCGATTTAATCAATGCAGGTAAGGAAACC
GTTACAACCATTCCAGGTTCTTCCATTTTCTCAAGTGCTGATTCATTCGCAATGATTCGT
GGTGGTCATGTTGATTTAACAATTCTTGGTGGTATGCAAGTTAGTGGTAATGGTGATTTG
GCCAATTGGGTCATTCCTGGCTCAATGGTTAAAGGTCCAGGTGGTGCTATGGATTTAACT
TCAAGTGGTTCACGTGTAGTTGTCATAATGGAACATACCACAAAACATGGAAAACCAAAG
ATTTTAAAGAATTGTACACTTCCACTCACTGGTAAATCTTGTGTAAATAGAATCATCACT
GAATTGGCAGTTTTCGATGTCGTCAATGGTAAATTAGAATTAATTGAAGTCGTTGATGGC
CAAACAGTTGATGATATTAAATCTAAAACTGAAGCAGAATTTACTGTTTCAAAAAATTTA
AAACCATTACAACAAGTTGAAATTTAAAATAATAATAATAATAATAAT
sequence update 2001.11.22
Translated Amino Acid sequence 
NLLLVISLKMLSRNLKTLVKNKNILYFTTSTSKSNKIYENAKEAVKDIPNGSKLLVGGFG
LCGIPENLISAVRDTGVKDLTVVSNNAGVDDFGLGVLLKSRQIKRMISSYVGENATFESQ
YLKGELEVELTPQGNLAERLRAGGAGIPAFYTSTGVGTVLVEEGGFPIKYANDG---

---EAAAKPKDEAAAKRERIVRRAALEFEDGMYCNLGIGMPTLASNFIPKGIRIELQSEN
GLLGMGPFPKMGEHDADLINAGKETVTTIPGSSIFSSADSFAMIRGGHVDLTILGGMQVS
GNGDLANWVIPGSMVKGPGGAMDLTSSGSRVVVIMEHTTKHGKPKILKNCTLPLTGKSCV
NRIITELAVFDVVNGKLELIEVVDGQTVDDIKSKTEAEFTVSKNLKPLQQVEI*nnnnnn
n
Translated Amino Acid sequence (All Frames) 
Frame A:
NLLLVISLKMLSRNLKTLVKNKNILYFTTSTSKSNKIYENAKEAVKDIPNGSKLLVGGFG
LCGIPENLISAVRDTGVKDLTVVSNNAGVDDFGLGVLLKSRQIKRMISSYVGENATFESQ
YLKGELEVELTPQGNLAERLRAGGAGIPAFYTSTGVGTVLVEEGGFPIKYANDG---

---EAAAKPKDEAAAKRERIVRRAALEFEDGMYCNLGIGMPTLASNFIPKGIRIELQSEN
GLLGMGPFPKMGEHDADLINAGKETVTTIPGSSIFSSADSFAMIRGGHVDLTILGGMQVS
GNGDLANWVIPGSMVKGPGGAMDLTSSGSRVVVIMEHTTKHGKPKILKNCTLPLTGKSCV
NRIITELAVFDVVNGKLELIEVVDGQTVDDIKSKTEAEFTVSKNLKPLQQVEI*nnnnnn
n

Frame B:
iyf*lyp*rcyqei*kl*lkikiyyillhqlvnqikfmkmqrkqlkifqmvqny**vdld
fvvfqri*yqqyviqv*ri*qllvimqvlmild*vfy*rvdklke*shhmlvrmqplrvn
isrvslk*s*hhkvi*qrd*eqvvqvfqlsiqvqvlvqcwwkkvvsqssmqmmv---

---kqlpnqrmrqqpresvsyvvql*slkmvcivilvlvcqhwpvisfqrvlesnynqrm
ay*vwvhsqkwanmmpi*smqvrkplqpfqvlpfsqvlihsq*fvvvmli*qflvvcklv
vmviwpigsflaqwlkvqvvlwi*lqvvhv*ls*wnipqnmenqrf*rivhfhslvnlv*
iesslnwqfsmssmvn*n*lkslmakqlmilnlklkqnllfqki*nhynklkfkiiiiii

Frame C:
ftfsyilkdvikkfknfs*k*kyiifyyin**ik*nl*kckgss*ryskwfkiisrwiwt
lwysrefnisst*yrckgfnsc***crc**fwircfike*tn*kndliicw*ecnl*esi
sqg*a*srvnttr*fsreiksrwcryssflykyrcwysvggrrwfpnqvck*wf---

---sscqtkg*gssqerayrtscsfrv*rwyvl*swywyanigq*fhskgy*nritirew
lirygsipkngrt*crfnqcr*gnrynhsrffhflkc*firndswwsc*fnnswwyas*w
*w*fgqlghswlng*rsrwcygfnfkwftcschngtyhktwktkdfkelytsthw*ilck
*nhh*igsfrcrqw*irin*sr*wpns**y*i*n*sriycfkkfktitts*nlk******
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFJ203 (VFJ203Q) /CSM/VF/VFJ2-A/VFJ203Q.Seq.d/                       2373   0.0
CFF695 (CFF695Q) /CSM/CF/CFF6-D/CFF695Q.Seq.d/                       1370   0.0
AFK785 (AFK785Q) /CSM/AF/AFK7-D/AFK785Q.Seq.d/                       1358   0.0
VFG118 (VFG118Q) /CSM/VF/VFG1-A/VFG118Q.Seq.d/                       1350   0.0
AFE843 (AFE843Q) /CSM/AF/AFE8-B/AFE843Q.Seq.d/                       1350   0.0
VFO838 (VFO838Q) /CSM/VF/VFO8-B/VFO838Q.Seq.d/                       1318   0.0
SLH304 (SLH304Q) /CSM/SL/SLH3-A/SLH304Q.Seq.d/                       1304   0.0
VFM456 (VFM456Q) /CSM/VF/VFM4-C/VFM456Q.Seq.d/                       1302   0.0
VSE557 (VSE557Q) /CSM/VS/VSE5-C/VSE557Q.Seq.d/                       1160   0.0
SLE479 (SLE479Q) /CSM/SL/SLE4-D/SLE479Q.Seq.d/                       1136   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ432556) Dictyostelium discoideum cDNA clone:ddv19e02, 3' ...  1372   0.0    1
(BJ372511) Dictyostelium discoideum cDNA clone:ddc12m24, 3' ...  1370   0.0    1
(BJ343373) Dictyostelium discoideum cDNA clone:dda22j21, 3' ...  1354   0.0    1
(BJ339771) Dictyostelium discoideum cDNA clone:dda10f12, 3' ...  1350   0.0    1
(BJ431146) Dictyostelium discoideum cDNA clone:ddv13c05, 3' ...  1344   0.0    1
(AU039262) Dictyostelium discoideum slug cDNA, clone SLH304.     1304   0.0    1
(BJ434993) Dictyostelium discoideum cDNA clone:ddv25p14, 3' ...  1300   0.0    1
(BJ436214) Dictyostelium discoideum cDNA clone:ddv30l10, 3' ...  1213   0.0    2
(AU263197) Dictyostelium discoideum vegetative cDNA clone:VS...  1160   0.0    1
(AU035007) Dictyostelium discoideum slug cDNA, clone SLE479.     1136   0.0    1
dna update 2009. 1.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54JD9) RecName: Full=Probable succinyl-CoA:3-ketoacid-coenzyme...   427   e-118
BC080373_1(BC080373|pid:none) Xenopus tropicalis oxct1 protein, ...   286   1e-75
AK312327_1(AK312327|pid:none) Homo sapiens cDNA, FLJ92639, highl...   283   9e-75
BC042239_1(BC042239|pid:none) Xenopus laevis hypothetical protei...   283   9e-75
BC124298_1(BC124298|pid:none) Danio rerio 3-oxoacid CoA transfer...   282   2e-74
CR457049_1(CR457049|pid:none) Homo sapiens full open reading fra...   281   3e-74
AK299668_1(AK299668|pid:none) Homo sapiens cDNA FLJ51747 complet...   281   4e-74
(Q9D0K2) RecName: Full=Succinyl-CoA:3-ketoacid-coenzyme A transf...   280   6e-74
CR860738_1(CR860738|pid:none) Pongo abelii mRNA; cDNA DKFZp459B1...   280   6e-74
AB172465_1(AB172465|pid:none) Macaca fascicularis brain cDNA clo...   280   1e-73
protein update 2009. 6.22
PSORT 

 psg: 0.60  gvh: 0.47  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: mitochondrial
	 28.0 %: nuclear
	 16.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for VFJ203 is mit
5' end seq. ID VFJ203F
5' end seq. 
>VFJ203F.Seq
AATTTACTTTTAGTTATATCCTTAAAGATGTTATCAAGAAATTTAAAAACTTTAGTTAAA
AATAAAAATATATTATATTTTACTACATCAACTAGTAAATCAAATAAAATTTATGAAAAT
GCAAAGGAAGCAGTTAAAGATATTCCAAATGGTTCAAAATTATTAGTAGGTGGATTTGGA
CTTTGTGGTATTCCAGAGAATTTAATATCAGCAGTACGTGATACAGGTGTAAAGGATTTA
ACAGTTGTTAGTAATAATGCAGGTGTTGATGATTTTGGATTAGGTGTTTTATTAAAGAGT
AGACAAATTAAAAGAATGATCTCATCATATGTTGGTGAGAATGCAACCTTTGAGAGTCAA
TATCTCAAGGGTGAGCTTGAAGTAGAGTTAACACCACAAGGTAATTTAGCAGAGAGATTA
AGAGCAGGTGGTGCAGGTATTCCAGCTTTCTATACAAGTACAGGTGTTGGTACAGTGTTG
GTGGAAGAAGGTGGTTTCCCAATCAAGTATGCAAATGATGGTTCNNNNNNNNNN
Length of 5' end seq. 534
3' end seq. ID VFJ203Z
3' end seq. 
>VFJ203Z.Seq
NNNNNNNNNNGAAGCAGCTGCCAAACCAAAGGATGAGGCAGCAGCCAAGAGAGAGCGTAT
CGTACGTCGTGCAGCTTTAGAGTTTGAAGATGGTATGTATTGTAATCTTGGTATTGGTAT
GCCAACATTGGCCAGTAATTTCATTCCAAAGGGTATTAGAATCGAATTACAATCAGAGAA
TGGCTTATTAGGTATGGGTCCATTCCCAAAAATGGGCGAACATGATGCCGATTTAATCAA
TGCAGGTAAGGAAACCGTTACAACCATTCCAGGTTCTTCCATTTTCTCAAGTGCTGATTC
ATTCGCAATGATTCGTGGTGGTCATGTTGATTTAACAATTCTTGGTGGTATGCAAGTTAG
TGGTAATGGTGATTTGGCCAATTGGGTCATTCCTGGCTCAATGGTTAAAGGTCCAGGTGG
TGCTATGGATTTAACTTCAAGTGGTTCACGTGTAGTTGTCATAATGGAACATACCACAAA
ACATGGAAAACCAAAGATTTTAAAGAATTGTACACTTCCACTCACTGGTAAATCTTGTGT
AAATAGAATCATCACTGAATTGGCAGTTTTCGATGTCGTCAATGGTAAATTAGAATTAAT
TGAAGTCGTTGATGGCCAAACAGTTGATGATATTAAATCTAAAACTGAAGCAGAATTTAC
TGTTTCAAAAAATTTAAAACCATTACAACAAGTTGAAATTTAAAATAATAATAATAATAA
TAAT
Length of 3' end seq. 724
Connected seq. ID VFJ203P
Connected seq. 
>VFJ203P.Seq
AATTTACTTTTAGTTATATCCTTAAAGATGTTATCAAGAAATTTAAAAACTTTAGTTAAA
AATAAAAATATATTATATTTTACTACATCAACTAGTAAATCAAATAAAATTTATGAAAAT
GCAAAGGAAGCAGTTAAAGATATTCCAAATGGTTCAAAATTATTAGTAGGTGGATTTGGA
CTTTGTGGTATTCCAGAGAATTTAATATCAGCAGTACGTGATACAGGTGTAAAGGATTTA
ACAGTTGTTAGTAATAATGCAGGTGTTGATGATTTTGGATTAGGTGTTTTATTAAAGAGT
AGACAAATTAAAAGAATGATCTCATCATATGTTGGTGAGAATGCAACCTTTGAGAGTCAA
TATCTCAAGGGTGAGCTTGAAGTAGAGTTAACACCACAAGGTAATTTAGCAGAGAGATTA
AGAGCAGGTGGTGCAGGTATTCCAGCTTTCTATACAAGTACAGGTGTTGGTACAGTGTTG
GTGGAAGAAGGTGGTTTCCCAATCAAGTATGCAAATGATGGTTC----------GAAGCA
GCTGCCAAACCAAAGGATGAGGCAGCAGCCAAGAGAGAGCGTATCGTACGTCGTGCAGCT
TTAGAGTTTGAAGATGGTATGTATTGTAATCTTGGTATTGGTATGCCAACATTGGCCAGT
AATTTCATTCCAAAGGGTATTAGAATCGAATTACAATCAGAGAATGGCTTATTAGGTATG
GGTCCATTCCCAAAAATGGGCGAACATGATGCCGATTTAATCAATGCAGGTAAGGAAACC
GTTACAACCATTCCAGGTTCTTCCATTTTCTCAAGTGCTGATTCATTCGCAATGATTCGT
GGTGGTCATGTTGATTTAACAATTCTTGGTGGTATGCAAGTTAGTGGTAATGGTGATTTG
GCCAATTGGGTCATTCCTGGCTCAATGGTTAAAGGTCCAGGTGGTGCTATGGATTTAACT
TCAAGTGGTTCACGTGTAGTTGTCATAATGGAACATACCACAAAACATGGAAAACCAAAG
ATTTTAAAGAATTGTACACTTCCACTCACTGGTAAATCTTGTGTAAATAGAATCATCACT
GAATTGGCAGTTTTCGATGTCGTCAATGGTAAATTAGAATTAATTGAAGTCGTTGATGGC
CAAACAGTTGATGATATTAAATCTAAAACTGAAGCAGAATTTACTGTTTCAAAAAATTTA
AAACCATTACAACAAGTTGAAATTTAAAATAATAATAATAATAATAAT
Length of connected seq. 1238
Full length Seq ID -
Full length Seq. -
Length of full length seq. -