>VFJ144 (VFJ144Q) /CSM/VF/VFJ1-B/VFJ144Q.Seq.d/
XXXXXXXXXXCTGGTATTGTTCGTAAGTATTTATAAATAAAAATATTCATTATTATTATT
ATTATTATTAATTACTAACATTATAATAATAATAATACCAATAATATACCAATGATAATG
ATAATAATGATAATGATAATAATGATAATAATGATAATAATGATAATAATGATAATAATA
ATAATAATAATATTAATGATAATGATGATAATGATGATAATATTAATAAATGATGATAAA
ATTCAATAGCGGATTCAAAATCACAAGCAAGTAGAGCAATTCAAGAATGTGCAACATTCC
ATCATAAATTTGGTTACCAAATTCCACCAAACGTTTTAGCAAAGAGAATGGCTGATATTG
CTCAACTTTCAACACAACACGCTTCAGCACGTCCATTAGGTGTTGCTATGATTCTTATTG
GTATTGATGATGAATTAGGTCCACAACTTTTCAAAGTAGATCCAGCTGGTGTTTTTACAG
GTTATAAAGCAACTGCTGCTGGTGAAAAAGAACAAGAATCAACAAATTTCCTCGAGAAAA
AGTTTAAATCTAATCCACAATTATCAAAAGATGAAACCATTCAAATGGCAATTTCAACAT
TACAAAGTGTACTTGGAGCAGATCTTAAACCATCAGATTTAGAGATTGGTATTTGTACAA
TGGATAATAGAAAGTTTGTTATTATGACTGATGAAGAT

---wycs*vfinknihyyyyyy*lltl****yq*ytndndnndndnndnndnndnndnnn
nnnindnddnddnink***NSIADSKSQASRAIQECATFHHKFGYQIPPNVLAKRMADIA
QLSTQHASARPLGVAMILIGIDDELGPQLFKVDPAGVFTGYKATAAGEKEQESTNFLEKK
FKSNPQLSKDETIQMAISTLQSVLGADLKPSDLEIGICTMDNRKFVIMTDED

Frame A:
---wycs*vfinknihyyyyyy*lltl****yq*ytndndnndndnndnndnndnndnnn
nnnindnddnddnink***NSIADSKSQASRAIQECATFHHKFGYQIPPNVLAKRMADIA
QLSTQHASARPLGVAMILIGIDDELGPQLFKVDPAGVFTGYKATAAGEKEQESTNFLEKK
FKSNPQLSKDETIQMAISTLQSVLGADLKPSDLEIGICTMDNRKFVIMTDED


Frame B:
---givrkyl*ikifiiiiiiiny*hynnnntnnipmimiimimiimiimiimiimiiii
iiilmimmimmiilinddkiq*riqnhkqveqfknvqhsiinlvtkfhqtf*qrewlill
nfqhntlqhvh*vll*fllvlmmn*vhnfsk*iqlvflqvikqlllvkknknqqissrks
lnlihnyqkmkpfkwqfqhykvyleqilnhqi*rlvfvqwiieslll*lmk


Frame C:
---vlfvsiyk*kyslllllllitniiiiiipiiyq************************
**y*********y**mmikfnsgfkitsk*snsrmcnips*iwlpnstkrfskeng*ycs
tfnttrfstsirccydsywy***irsttfqsrsswcfyrl*snccw*krtrinkfprekv
*i*stiikr*nhsngnfnitkctwsrs*tirfrdwylyng**kvcyyd**r

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFJ144 (VFJ144Q) /CSM/VF/VFJ1-B/VFJ144Q.Seq.d/                        910   0.0
VSB235 (VSB235Q) /CSM/VS/VSB2-B/VSB235Q.Seq.d/                        882   0.0
SSC845 (SSC845Q) /CSM/SS/SSC8-B/SSC845Q.Seq.d/                        868   0.0
SSA581 (SSA581Q) /CSM/SS/SSA5-D/SSA581Q.Seq.d/                        710   0.0
SHI806 (SHI806Q) /CSM/SH/SHI8-A/SHI806Q.Seq.d/                        565   e-160
VSF611 (VSF611Q) /CSM/VS/VSF6-A/VSF611Q.Seq.d/                        420   e-116
VSH153 (VSH153Q) /CSM/VS/VSH1-C/VSH153Q.Seq.d/                        375   e-103
SLI877 (SLI877Q) /CSM/SL/SLI8-D/SLI877Q.Seq.d/                         38   0.17
AFE545 (AFE545Q) /CSM/AF/AFE5-B/AFE545Q.Seq.d/                         38   0.17
VHO709 (VHO709Q) /CSM/VH/VHO7-A/VHO709Q.Seq.d/                         36   0.69

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ432654) Dictyostelium discoideum cDNA clone:ddv19g11, 3' ...   892   0.0    2
(AU261910) Dictyostelium discoideum vegetative cDNA clone:VS...   882   0.0    1
(AU051985) Dictyostelium discoideum slug cDNA, clone SSC845.      781   0.0    2
(C83913) Dictyostelium discoideum slug cDNA, clone SSA581.        710   0.0    1
(BJ396038) Dictyostelium discoideum cDNA clone:dds41l02, 5' ...   565   e-157  1
(AU265497) Dictyostelium discoideum vegetative cDNA clone:VS...   420   e-113  1
(AU266985) Dictyostelium discoideum vegetative cDNA clone:VS...   375   2e-99  1
(CB338989) pl37d06.y1 Ancylostoma ceylanicum adult Ancylosto...    48   9e-04  2
(ES497183) BIG_AF_4999 Brine Shrimp Adult (20 days after hat...    38   0.19   2
(AZ931473) 474.dhz84g04.s1 Saccharomyces unisporus NRRL Y-15...    32   0.56   3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54XM7) RecName: Full=Proteasome subunit alpha type-6;         ...   287   2e-76
EF083048_1(EF083048|pid:none) Picea sitchensis clone WS02716_P20...   169   6e-41
DQ895551_1(DQ895551|pid:none) Synthetic construct Homo sapiens c...   165   1e-39
(P60900) RecName: Full=Proteasome subunit alpha type-6;         ...   165   1e-39
X61972_1(X61972|pid:none) H.sapiens mRNA for macropain subunit i...   165   1e-39
DQ213268_1(DQ213268|pid:none) Taeniopygia guttata clone 0061P001...   164   2e-39
BT046488_1(BT046488|pid:none) Salmo salar clone ssal-evf-541-338...   163   4e-39
BT043852_1(BT043852|pid:none) Salmo salar clone HM4_0626 proteas...   163   5e-39
AC146756_8(AC146756|pid:none) Medicago truncatula clone mth2-15l...   162   7e-39
BT049977_1(BT049977|pid:none) Salmo salar clone ssal-evf-506-248...   162   7e-39

 psg: 0.62  gvh: 0.50  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 28.0 %: nuclear
	 24.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VFJ144 is cyt

>VFJ144Z.Seq
NNNNNNNNNNCTGGTATTGTTCGTAAGTATTTATAAATAAAAATATTCATTATTATTATT
ATTATTATTAATTACTAACATTATAATAATAATAATACCAATAATATACCAATGATAATG
ATAATAATGATAATGATAATAATGATAATAATGATAATAATGATAATAATGATAATAATA
ATAATAATAATATTAATGATAATGATGATAATGATGATAATATTAATAAATGATGATAAA
ATTCAATAGCGGATTCAAAATCACAAGCAAGTAGAGCAATTCAAGAATGTGCAACATTCC
ATCATAAATTTGGTTACCAAATTCCACCAAACGTTTTAGCAAAGAGAATGGCTGATATTG
CTCAACTTTCAACACAACACGCTTCAGCACGTCCATTAGGTGTTGCTATGATTCTTATTG
GTATTGATGATGAATTAGGTCCACAACTTTTCAAAGTAGATCCAGCTGGTGTTTTTACAG
GTTATAAAGCAACTGCTGCTGGTGAAAAAGAACAAGAATCAACAAATTTCCTCGAGAAAA
AGTTTAAATCTAATCCACAATTATCAAAAGATGAAACCATTCAAATGGCAATTTCAACAT
TACAAAGTGTACTTGGAGCAGATCTTAAACCATCAGATTTAGAGATTGGTATTTGTACAA
TGGATAATAGAAAGTTTGTTATTATGACTGATGAAGAT