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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFJ138
Library VF
(Link to library)
Clone ID VFJ138
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16605-1
Original site URL
Representative seq. ID VFJ138P
(Link to Original site)
Representative DNA sequence 
>VFJ138 (VFJ138Q) /CSM/VF/VFJ1-B/VFJ138Q.Seq.d/
AATNAATATATATATACTAAAAATGAGAGTTTTATNTTCCTTTGTTTATTATTAGTTAGC
TACGCTTCTGCTAAACAACAATTCTCTGAATTACAATACAGAAATGCTTTCACCAACTGG
ATGCAAGCTCACCAAAGAACTTATTCCTCTGAAGAATTTAATGCTCGTTATCAAATCTTC
AAATCCAATATGGATTATGTACACCAATGGAATTCAAAAGGTGGTGAAACCGTTTTGGGT
TTAAATGTTTTCGCTGATATTACCAACCAAGAATATAGAACTACCTACTTGGGTACCCCA
TTCGATGGTTCAGCCCTCATTGGTACTGAAGAAGAGAAAATCTTCTCCACCCCAGCCCCA
ACTGTTGATTGGAGAGCTCAAGGTGCTGTCACACCAATTAAAAATCAAGGTCAATGTGGT
GGCTGCTGGTCATTCTCAACCACTGGTTCAACTGAAGGTGCTCACTTTATTGCATCTGGA
ACAAAAAAAGATTTAGTTTCATTATCTGAACAAAACTTGATCGATTGTTCAAAATCATAC
GGTAACAATGGTTGXXXXXXXXXXAAAACTTNATCCTCATCATCATCAGGTAAAGCTTNA
TNATNATNATCAGGCAAAGCTTNATCATNATCATCATCAGGTAAAACTTNATTTGNTGCT
TCATCAACCTNTGGTTTTCAATCAGGTTNCCAATCAGGTAGCCNATCAGGCCNATCCCCC
GGTTNNCAATCAGGTCAAACCTNTGCTTNTGGTCAAGCATCAGCANCAGGTTCTGGTTNT
GGCNCAGGTTCAGGTTCAGGNTCAGGTTCAGGCNCAGGNGCTGTTTAGGCCNCATCTGGN
AANTACTGGATCGTTAAAAACNCATGGGGNACTTCATGGGGNATGGATGGTTACNTTTTT
ATGAGCAAANATANAAANAACNATTNTGGNATCGCAACAANGGCTTCTTTCCCNACTGCC
NCNNCAAATTAAAATTTTATTTTTAATTG
sequence update 2001.11.22
Translated Amino Acid sequence 
NXYIYTKNESFXFLCLLLVSYASAKQQFSELQYRNAFTNWMQAHQRTYSSEEFNARYQIF
KSNMDYVHQWNSKGGETVLGLNVFADITNQEYRTTYLGTPFDGSALIGTEEEKIFSTPAP
TVDWRAQGAVTPIKNQGQCGGCWSFSTTGSTEGAHFIASGTKKDLVSLSEQNLIDCSKSY
GNNG---

---KLXPHHHQVKLXXXXQAKLXHXHHQVKLXLXLHQPXVFNQVXNQVAXQAXPPVXNQV
KPXLXVKHQXQVLVXAQVQVQXQVQAQXLFRPHLXXTGSLKTHGXLHGXWMVTFL*axix
xtixxsqqxllsxlpxqikilfli
Translated Amino Acid sequence (All Frames) 
Frame A:
NXYIYTKNESFXFLCLLLVSYASAKQQFSELQYRNAFTNWMQAHQRTYSSEEFNARYQIF
KSNMDYVHQWNSKGGETVLGLNVFADITNQEYRTTYLGTPFDGSALIGTEEEKIFSTPAP
TVDWRAQGAVTPIKNQGQCGGCWSFSTTGSTEGAHFIASGTKKDLVSLSEQNLIDCSKSY
GNNG---

---ktxsssssgkaxxxxsgkaxsxsssgktxfxasstxgfqsgxqsgsxsgxspgxqsg
qtxaxgqasaxgsgxgxgsgsgsgsgxgav*axsgxywivknxwgtswgmdgyxfmskxx
xnxxgiatxasfptaxxn*nfifn

Frame B:
xniyilkmrvlxsfvyy*latlllnnnslnyntemlsptgckltkeliplknlmlvikss
npiwimytngiqkvvkpfwv*mfslilptknielptwvphsmvqpslvlkkrkssppqpq
lligelkvlshqlkikvnvvaaghsqplvqlkvltllhleqkki*fhylnkt*sivqnht
vtmv---

---KLXPHHHQVKLXXXXQAKLXHXHHQVKLXLXLHQPXVFNQVXNQVAXQAXPPVXNQV
KPXLXVKHQXQVLVXAQVQVQXQVQAQXLFRPHLXXTGSLKTHGXLHGXWMVTFL*axix
xtixxsqqxllsxlpxqikilfli

Frame C:
xiyiy*k*efyxplfiis*lrfc*ttil*itiqkcfhqldasspknlfl*ri*cslsnlq
iqyglctpmefkrw*nrfgfkcfr*yyqpri*nyllgypirwfsphwy*rrenllhpspn
c*lessrcchtn*ksrsmwwllvilnhwfn*rcslyciwnkkrfsfii*tkldrlfkiir
*qwl---

---nxiliiir*sxxxxirqsxixiiir*nxixcfinlwfsirxpir*pirpiprxxirs
nlcxwssisxrfwxwxrfrfrxrfrxrxclgxiwxxldr*kxmgxfmgxgwlxfyeqxxk
xxxwxrnxgffpxcxxklkfyf*l
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFJ138 (VFJ138Q) /CSM/VF/VFJ1-B/VFJ138Q.Seq.d/                       1604   0.0
VFB381 (VFB381Q) /CSM/VF/VFB3-D/VFB381Q.Seq.d/                       1043   0.0
VFA591 (VFA591Q) /CSM/VF/VFA5-D/VFA591Q.Seq.d/                       1033   0.0
VFA246 (VFA246Q) /CSM/VF/VFA2-B/VFA246Q.Seq.d/                       1033   0.0
VFO623 (VFO623Q) /CSM/VF/VFO6-A/VFO623Q.Seq.d/                       1029   0.0
VFO306 (VFO306Q) /CSM/VF/VFO3-A/VFO306Q.Seq.d/                       1029   0.0
VFN628 (VFN628Q) /CSM/VF/VFN6-B/VFN628Q.Seq.d/                       1029   0.0
VFN393 (VFN393Q) /CSM/VF/VFN3-D/VFN393Q.Seq.d/                       1029   0.0
VFN294 (VFN294Q) /CSM/VF/VFN2-D/VFN294Q.Seq.d/                       1029   0.0
VFM872 (VFM872Q) /CSM/VF/VFM8-C/VFM872Q.Seq.d/                       1029   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ414502) Dictyostelium discoideum cDNA clone:ddv19k09, 5' ...   944   0.0    2
(BJ411286) Dictyostelium discoideum cDNA clone:ddv3b21, 5' e...   942   0.0    2
(L36204) Dictyostelium discoideum cysteine proteinase (CP4) ...   884   0.0    5
(BJ411046) Dictyostelium discoideum cDNA clone:ddv2e23, 5' e...   932   0.0    2
(BJ410659) Dictyostelium discoideum cDNA clone:ddv1k12, 5' e...   932   0.0    2
(BJ416557) Dictyostelium discoideum cDNA clone:ddv26e24, 5' ...   884   0.0    3
(BJ416649) Dictyostelium discoideum cDNA clone:ddv26h02, 5' ...   884   0.0    3
(BJ415773) Dictyostelium discoideum cDNA clone:ddv23n16, 5' ...   884   0.0    3
(BJ415276) Dictyostelium discoideum cDNA clone:ddv22e06, 5' ...   884   0.0    3
(BJ410402) Dictyostelium discoideum cDNA clone:ddv12l09, 5' ...   884   0.0    3
dna update 2009. 1.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

L36204_1(L36204|pid:none) Dictyostelium discoideum cysteine prot...   358   1e-97
(Q94504) RecName: Full=Cysteine proteinase 7;          EC=3.4.22...   275   2e-72
(P54640) RecName: Full=Cysteine proteinase 5;          EC=3.4.22...   266   8e-70
(Q94503) RecName: Full=Cysteine proteinase 6;          EC=3.4.22...   266   1e-69
L36205_1(L36205|pid:none) Dictyostelium discoideum cysteine prot...   259   8e-68
(P04989) RecName: Full=Cysteine proteinase 2;          EC=3.4.22...   189   1e-46
EF053509_1(EF053509|pid:none) Acanthamoeba castellanii cysteine ...   160   5e-38
AC117076_20(AC117076|pid:none) Dictyostelium discoideum chromoso...   152   2e-35
AK226753_1(AK226753|pid:none) Arabidopsis thaliana mRNA for papa...   136   1e-30
AC000132_24(AC000132|pid:none) Sequence of BAC F21M12 from Arabi...   136   1e-30
protein update 2009. 6.21
PSORT 

 psg: 0.97  gvh: 0.88  alm: 0.53  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: extracellular, including cell wall
	 16.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for VFJ138 is exc
5' end seq. ID VFJ138F
5' end seq. 
>VFJ138F.Seq
AATNAATATATATATACTAAAAATGAGAGTTTTATNTTCCTTTGTTTATTATTAGTTAGC
TACGCTTCTGCTAAACAACAATTCTCTGAATTACAATACAGAAATGCTTTCACCAACTGG
ATGCAAGCTCACCAAAGAACTTATTCCTCTGAAGAATTTAATGCTCGTTATCAAATCTTC
AAATCCAATATGGATTATGTACACCAATGGAATTCAAAAGGTGGTGAAACCGTTTTGGGT
TTAAATGTTTTCGCTGATATTACCAACCAAGAATATAGAACTACCTACTTGGGTACCCCA
TTCGATGGTTCAGCCCTCATTGGTACTGAAGAAGAGAAAATCTTCTCCACCCCAGCCCCA
ACTGTTGATTGGAGAGCTCAAGGTGCTGTCACACCAATTAAAAATCAAGGTCAATGTGGT
GGCTGCTGGTCATTCTCAACCACTGGTTCAACTGAAGGTGCTCACTTTATTGCATCTGGA
ACAAAAAAAGATTTAGTTTCATTATCTGAACAAAACTTGATCGATTGTTCAAAATCATAC
GGTAACAATGGTTGNNNNNNNNNN
Length of 5' end seq. 564
3' end seq. ID VFJ138Z
3' end seq. 
>VFJ138Z.Seq
NNNNNNNNNNAAAACTTNATCCTCATCATCATCAGGTAAAGCTTNATNATNATNATCAGG
CAAAGCTTNATCATNATCATCATCAGGTAAAACTTNATTTGNTGCTTCATCAACCTNTGG
TTTTCAATCAGGTTNCCAATCAGGTAGCCNATCAGGCCNATCCCCCGGTTNNCAATCAGG
TCAAACCTNTGCTTNTGGTCAAGCATCAGCANCAGGTTCTGGTTNTGGCNCAGGTTCAGG
TTCAGGNTCAGGTTCAGGCNCAGGNGCTGTTTAGGCCNCATCTGGNAANTACTGGATCGT
TAAAAACNCATGGGGNACTTCATGGGGNATGGATGGTTACNTTTTTATGAGCAAANATAN
AAANAACNATTNTGGNATCGCAACAANGGCTTCTTTCCCNACTGCCNCNNCAAATTAAAA
TTTTATTTTTAATTG
Length of 3' end seq. 435
Connected seq. ID VFJ138P
Connected seq. 
>VFJ138P.Seq
AATNAATATATATATACTAAAAATGAGAGTTTTATNTTCCTTTGTTTATTATTAGTTAGC
TACGCTTCTGCTAAACAACAATTCTCTGAATTACAATACAGAAATGCTTTCACCAACTGG
ATGCAAGCTCACCAAAGAACTTATTCCTCTGAAGAATTTAATGCTCGTTATCAAATCTTC
AAATCCAATATGGATTATGTACACCAATGGAATTCAAAAGGTGGTGAAACCGTTTTGGGT
TTAAATGTTTTCGCTGATATTACCAACCAAGAATATAGAACTACCTACTTGGGTACCCCA
TTCGATGGTTCAGCCCTCATTGGTACTGAAGAAGAGAAAATCTTCTCCACCCCAGCCCCA
ACTGTTGATTGGAGAGCTCAAGGTGCTGTCACACCAATTAAAAATCAAGGTCAATGTGGT
GGCTGCTGGTCATTCTCAACCACTGGTTCAACTGAAGGTGCTCACTTTATTGCATCTGGA
ACAAAAAAAGATTTAGTTTCATTATCTGAACAAAACTTGATCGATTGTTCAAAATCATAC
GGTAACAATGGTTG----------AAAACTTNATCCTCATCATCATCAGGTAAAGCTTNA
TNATNATNATCAGGCAAAGCTTNATCATNATCATCATCAGGTAAAACTTNATTTGNTGCT
TCATCAACCTNTGGTTTTCAATCAGGTTNCCAATCAGGTAGCCNATCAGGCCNATCCCCC
GGTTNNCAATCAGGTCAAACCTNTGCTTNTGGTCAAGCATCAGCANCAGGTTCTGGTTNT
GGCNCAGGTTCAGGTTCAGGNTCAGGTTCAGGCNCAGGNGCTGTTTAGGCCNCATCTGGN
AANTACTGGATCGTTAAAAACNCATGGGGNACTTCATGGGGNATGGATGGTTACNTTTTT
ATGAGCAAANATANAAANAACNATTNTGGNATCGCAACAANGGCTTCTTTCCCNACTGCC
NCNNCAAATTAAAATTTTATTTTTAATTG
Length of connected seq. 979
Full length Seq ID -
Full length Seq. -
Length of full length seq. -