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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFM712
Library AF
(Link to library)
Clone ID AFM712
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFM712P
(Link to Original site)
Representative DNA sequence 
>AFM712 (AFM712Q) /CSM/AF/AFM7-A/AFM712Q.Seq.d/
ATATCAGCAGCTAAAAAATTTGGTAGAAAAGGTATTAGAACTTTAGGTGATAATGAAGTT
TTATTATCCGATGGTGCTATTAGAGGTACAGTAACCGATACTCATCGTGTATTTTACGGT
ATTCCGTTTGCCCGTCCACCAATTGACGAATTACGTTATGAAGATCCACAACCACCAAAG
CCATGGTCCTATGTTAGAGATGGTACTAAACAAAGAGACCAATGTATTCAAGATTGTAAA
TTAGGTAAAGGAAGTTGTTCTGAAGTTGGTACAAGTGAAGATTGTCTTTATTTAGATGTA
TTCATTCCAAGAACAGTTAATCCAGGTTCAAAAGTACCAGTAATGGTTTTTATTCCAGGT
GGTGCATTTACACAAGGTACTGGTTCATGTCCACTTTATGATGGTCTTAAATTTGCAAAT
TCATCAGTTATTGTTGTAAATGTAAACTATCGTCTTGGTGTATTAGGTTTCTTATGTACT
GGTTTATTAAGTGGTAATTTTTGGTTTCTTAGATCAAGTTATGGCTTTAGATTGGGTTCA
AGAAAATXXXXXXXXXXGAGGAGATTTTAGCAATTCAAAAGGAACTTGGTTTGGCTATTG
GTGATAAGATTCTTGATGCTTTTACAATCTGGTCACCAGTGGTCGACGGAATTAATGTAA
ATGAACAACCATTGACAATGATAAAACAAGGTACAACTCATGATGTTCCAACCATTATTG
GTGATAACCAAGATGAGGCTATACTCTTTGTTTACATGACGTATAAAAATGTTGTGATTC
CAAGTTCCTATAGAACCATGGTACATGTTTTATTCGGTATTGCAAATGGTAATAAAGTTT
TAGAGCATTATCCATTACCAGGTTTCTTAAAAGATAGTAGACCAATATTATCTAAATTAC
TCACCGATTACCTCTTCCGTTGTCCAGGTAGATACCATGTCTCAAAATCAGCTCAAGCCA
ATGAATCACCAATTTACCATTACCAATACAAACAAGTATTATCAGGAGGTCATTCATTTG
AAGCATGTGAAGGTTTAGTTTGTCATGGTACTGAATTACCAATGGTTTTCAATACATATG
AAAGTGCACTTGATTTAGATTTAGAAGAGGAAGAAGAAGAATTTGCTGAACAATTAAATA
ATTATTTCGTTAATTTCATTAAATACTCTAATCCAAGTCATCCTAATGGTTTACCAACTC
CAAAAGTTTGGAATCCAACCACTAAAACTACCAATACCTCTTT
sequence update 2009. 4. 3
Translated Amino Acid sequence 
ISAAKKFGRKGIRTLGDNEVLLSDGAIRGTVTDTHRVFYGIPFARPPIDELRYEDPQPPK
PWSYVRDGTKQRDQCIQDCKLGKGSCSEVGTSEDCLYLDVFIPRTVNPGSKVPVMVFIPG
GAFTQGTGSCPLYDGLKFANSSVIVVNVNYRLGVLGFLCTGLLSGNFWFLRSSYGFRLGS
RK---

---EEILAIQKELGLAIGDKILDAFTIWSPVVDGINVNEQPLTMIKQGTTHDVPTIIGDN
QDEAILFVYMTYKNVVIPSSYRTMVHVLFGIANGNKVLEHYPLPGFLKDSRPILSKLLTD
YLFRCPGRYHVSKSAQANESPIYHYQYKQVLSGGHSFEACEGLVCHGTELPMVFNTYESA
LDLDLEEEEEEFAEQLNNYFVNFIKYSNPSHPNGLPTPKVWNPTTKTTNTS
Translated Amino Acid sequence (All Frames) 
Frame A:
ISAAKKFGRKGIRTLGDNEVLLSDGAIRGTVTDTHRVFYGIPFARPPIDELRYEDPQPPK
PWSYVRDGTKQRDQCIQDCKLGKGSCSEVGTSEDCLYLDVFIPRTVNPGSKVPVMVFIPG
GAFTQGTGSCPLYDGLKFANSSVIVVNVNYRLGVLGFLCTGLLSGNFWFLRSSYGFRLGS
RK---

---EEILAIQKELGLAIGDKILDAFTIWSPVVDGINVNEQPLTMIKQGTTHDVPTIIGDN
QDEAILFVYMTYKNVVIPSSYRTMVHVLFGIANGNKVLEHYPLPGFLKDSRPILSKLLTD
YLFRCPGRYHVSKSAQANESPIYHYQYKQVLSGGHSFEACEGLVCHGTELPMVFNTYESA
LDLDLEEEEEEFAEQLNNYFVNFIKYSNPSHPNGLPTPKVWNPTTKTTNTS

Frame B:
yqqlknlvekvlel*vimkfyypmvllevq*pilivyftvfrlpvhqltnyvmkihnhqs
hgpmlemvlnketnvfkivn*vkevvlklvqvkivfi*mysfqeqliqvqkyq*wflfqv
vhlhkvlvhvhfmmvlnlqihqlll*m*tivlvy*vsyvlvy*vvifgfldqvmaldwvq
en---

---rrf*qfkrnlvwllvirflmllqsghqwstelm*mnnh*q**nkvqlmmfqpllvit
kmrlyslft*rikml*fqvpiepwymfysvlqmvikf*siihyqvs*kivdqyylnyspi
tssvvqvdtmsqnqlkpmnhqftitntnkyyqevihlkhvkv*fvmvlnyqwfsihmkvh
li*i*krkkknllnn*iiislislntliqvilmvyqlqkfgiqplklpipl

Frame C:
iss*kiw*kry*nfr***sfiirwcy*rysnrysscilrysvcpstn*ritl*rstttka
mvlc*rwy*tkrpmysrl*ir*rklf*swyk*rlslfrcihskns*srfkstsngfysrw
ciytrywfmstl*ws*ickfisycckcklsswcirflmywfikw*flvs*iklwl*igfk
k---

---gdfsnskgtwfgyw**ds*cfynlvtsgrrn*ck*ttidndktryns*csnhyw**p
r*gytlclhdv*kccdskfl*nhgtcfiryckw**sfralsitrflkr**tnii*ithrl
plplsr*ipclkisssq*itnlplpiqtsiirrsfi*sm*rfslswy*itngfqyi*kct
*frfrrgrrric*tik*lfr*fh*il*skss*wftnskslesnh*nyqylf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFM712 (AFM712Q) /CSM/AF/AFM7-A/AFM712Q.Seq.d/                       2405   0.0
AFE272 (AFE272Q) /CSM/AF/AFE2-C/AFE272Q.Seq.d/                       1352   0.0
VFE213 (VFE213Q) /CSM/VF/VFE2-A/VFE213Q.Seq.d/                       1253   0.0
VFI771 (VFI771Q) /CSM/VF/VFI7-C/VFI771Q.Seq.d/                       1243   0.0
VFK316 (VFK316Q) /CSM/VF/VFK3-A/VFK316Q.Seq.d/                       1197   0.0
VFD731 (VFD731Q) /CSM/VF/VFD7-B/VFD731Q.Seq.d/                       1098   0.0
VFH802 (VFH802Q) /CSM/VF/VFH8-A/VFH802Q.Seq.d/                       1094   0.0
VFL195 (VFL195Q) /CSM/VF/VFL1-D/VFL195Q.Seq.d/                       1070   0.0
VFJ684 (VFJ684Q) /CSM/VF/VFJ6-D/VFJ684Q.Seq.d/                       1068   0.0
AFG615 (AFG615Q) /CSM/AF/AFG6-A/AFG615Q.Seq.d/                       1063   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(X52464) D.discoideum CP mRNA for crystal protein.               1358   0.0    1
(BJ347094) Dictyostelium discoideum cDNA clone:dda26h03, 3' ...  1358   0.0    1
(BJ342150) Dictyostelium discoideum cDNA clone:dda9o18, 3' e...  1261   0.0    1
(BJ430873) Dictyostelium discoideum cDNA clone:ddv9i04, 3' e...  1253   0.0    1
(BJ432441) Dictyostelium discoideum cDNA clone:ddv18n17, 3' ...  1243   0.0    1
(BJ433246) Dictyostelium discoideum cDNA clone:ddv21p03, 3' ...  1197   0.0    1
(BJ430711) Dictyostelium discoideum cDNA clone:ddv8n07, 3' e...  1098   0.0    1
(BJ329732) Dictyostelium discoideum cDNA clone:dda26h03, 5' ...  1084   0.0    1
(BJ434147) Dictyostelium discoideum cDNA clone:ddv16d02, 3' ...  1009   0.0    2
(BJ434114) Dictyostelium discoideum cDNA clone:ddv23m23, 3' ...  1070   0.0    1
dna update 2009. 4.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P21837) RecName: Full=Crystal protein; Flags: Precursor;  &A345...   447   e-124
AC117075_63(AC117075|pid:none) Dictyostelium discoideum chromoso...   212   5e-55
B34576(B34576) D2 protein precursor - slime mold (Dictyostelium ...   211   9e-55
(P18142) RecName: Full=cAMP-regulated D2 protein; Flags: Precurs...   211   1e-54
AK033563_1(AK033563|pid:none) Mus musculus adult male colon cDNA...   122   1e-28
AK077248_1(AK077248|pid:none) Mus musculus 11 days pregnant adul...   122   1e-28
BC107806_1(BC107806|pid:none) Rattus norvegicus similar to Carbo...   121   1e-28
CP001053_2587(CP001053|pid:none) Burkholderia phytofirmans PsJN ...   122   1e-28
BC058815_1(BC058815|pid:none) Mus musculus cDNA sequence BC01528...   119   5e-28
BC123305_1(BC123305|pid:none) Xenopus laevis cDNA clone IMAGE:70...   119   7e-28
protein update 2009. 4. 6
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for AFM712 is

5' end seq. ID AFM712F
5' end seq. 
>AFM712F.Seq
ATATCAGCAGCTAAAAAATTTGGTAGAAAAGGTATTAGAACTTTAGGTGATAATGAAGTT
TTATTATCCGATGGTGCTATTAGAGGTACAGTAACCGATACTCATCGTGTATTTTACGGT
ATTCCGTTTGCCCGTCCACCAATTGACGAATTACGTTATGAAGATCCACAACCACCAAAG
CCATGGTCCTATGTTAGAGATGGTACTAAACAAAGAGACCAATGTATTCAAGATTGTAAA
TTAGGTAAAGGAAGTTGTTCTGAAGTTGGTACAAGTGAAGATTGTCTTTATTTAGATGTA
TTCATTCCAAGAACAGTTAATCCAGGTTCAAAAGTACCAGTAATGGTTTTTATTCCAGGT
GGTGCATTTACACAAGGTACTGGTTCATGTCCACTTTATGATGGTCTTAAATTTGCAAAT
TCATCAGTTATTGTTGTAAATGTAAACTATCGTCTTGGTGTATTAGGTTTCTTATGTACT
GGTTTATTAAGTGGTAATTTTTGGTTTCTTAGATCAAGTTATGGCTTTAGATTGGGTTCA
AGAAAATXXXXXXXXXX
Length of 5' end seq. 557
3' end seq. ID AFM712Z
3' end seq. 
>AFM712Z.Seq
XXXXXXXXXXGAGGAGATTTTAGCAATTCAAAAGGAACTTGGTTTGGCTATTGGTGATAA
GATTCTTGATGCTTTTACAATCTGGTCACCAGTGGTCGACGGAATTAATGTAAATGAACA
ACCATTGACAATGATAAAACAAGGTACAACTCATGATGTTCCAACCATTATTGGTGATAA
CCAAGATGAGGCTATACTCTTTGTTTACATGACGTATAAAAATGTTGTGATTCCAAGTTC
CTATAGAACCATGGTACATGTTTTATTCGGTATTGCAAATGGTAATAAAGTTTTAGAGCA
TTATCCATTACCAGGTTTCTTAAAAGATAGTAGACCAATATTATCTAAATTACTCACCGA
TTACCTCTTCCGTTGTCCAGGTAGATACCATGTCTCAAAATCAGCTCAAGCCAATGAATC
ACCAATTTACCATTACCAATACAAACAAGTATTATCAGGAGGTCATTCATTTGAAGCATG
TGAAGGTTTAGTTTGTCATGGTACTGAATTACCAATGGTTTTCAATACATATGAAAGTGC
ACTTGATTTAGATTTAGAAGAGGAAGAAGAAGAATTTGCTGAACAATTAAATAATTATTT
CGTTAATTTCATTAAATACTCTAATCCAAGTCATCCTAATGGTTTACCAACTCCAAAAGT
TTGGAATCCAACCACTAAAACTACCAATACCTCTTT
Length of 3' end seq. 696
Connected seq. ID AFM712P
Connected seq. 
>AFM712P.Seq
ATATCAGCAGCTAAAAAATTTGGTAGAAAAGGTATTAGAACTTTAGGTGATAATGAAGTT
TTATTATCCGATGGTGCTATTAGAGGTACAGTAACCGATACTCATCGTGTATTTTACGGT
ATTCCGTTTGCCCGTCCACCAATTGACGAATTACGTTATGAAGATCCACAACCACCAAAG
CCATGGTCCTATGTTAGAGATGGTACTAAACAAAGAGACCAATGTATTCAAGATTGTAAA
TTAGGTAAAGGAAGTTGTTCTGAAGTTGGTACAAGTGAAGATTGTCTTTATTTAGATGTA
TTCATTCCAAGAACAGTTAATCCAGGTTCAAAAGTACCAGTAATGGTTTTTATTCCAGGT
GGTGCATTTACACAAGGTACTGGTTCATGTCCACTTTATGATGGTCTTAAATTTGCAAAT
TCATCAGTTATTGTTGTAAATGTAAACTATCGTCTTGGTGTATTAGGTTTCTTATGTACT
GGTTTATTAAGTGGTAATTTTTGGTTTCTTAGATCAAGTTATGGCTTTAGATTGGGTTCA
AGAAAAT----------GAGGAGATTTTAGCAATTCAAAAGGAACTTGGTTTGGCTATTG
GTGATAAGATTCTTGATGCTTTTACAATCTGGTCACCAGTGGTCGACGGAATTAATGTAA
ATGAACAACCATTGACAATGATAAAACAAGGTACAACTCATGATGTTCCAACCATTATTG
GTGATAACCAAGATGAGGCTATACTCTTTGTTTACATGACGTATAAAAATGTTGTGATTC
CAAGTTCCTATAGAACCATGGTACATGTTTTATTCGGTATTGCAAATGGTAATAAAGTTT
TAGAGCATTATCCATTACCAGGTTTCTTAAAAGATAGTAGACCAATATTATCTAAATTAC
TCACCGATTACCTCTTCCGTTGTCCAGGTAGATACCATGTCTCAAAATCAGCTCAAGCCA
ATGAATCACCAATTTACCATTACCAATACAAACAAGTATTATCAGGAGGTCATTCATTTG
AAGCATGTGAAGGTTTAGTTTGTCATGGTACTGAATTACCAATGGTTTTCAATACATATG
AAAGTGCACTTGATTTAGATTTAGAAGAGGAAGAAGAAGAATTTGCTGAACAATTAAATA
ATTATTTCGTTAATTTCATTAAATACTCTAATCCAAGTCATCCTAATGGTTTACCAACTC
CAAAAGTTTGGAATCCAACCACTAAAACTACCAATACCTCTTT
Length of connected seq. 1233
Full length Seq ID -
Full length Seq. -
Length of full length seq. -