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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFI730
Library VF
(Link to library)
Clone ID VFI730
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15330-1
Original site URL
Representative seq. ID VFI730E
(Link to Original site)
Representative DNA sequence 
>VFI730 (VFI730Q) /CSM/VF/VFI7-B/VFI730Q.Seq.d/
ACATATACACACACTCACACACTTAATCATATTTATATATATATTTTTTAATTAATAAAA
AATCATATATATATCTTATAAATTTGCTTTCATACAAATTAAAACCGTTATTATTAAATA
ACTTACATCATATAAATTTATACTTTTAGTATATCTTTTTCACTCTCATCTTTTTTTATT
CCCCATTTAAAAAATTCAAATTTAAATTGATAATTAATTAAAATTAAATTCAAAAAATGA
TTGTAACTCAAATTGCTGGTAAAATTCAAGTCATCTTTGGTCCAATGTTTAGCGGAAAGA
CCACTGAGCTCATTAGAAGAATTAAAAGATTTAATTTTGCAAACAAAAAATGCTTATTAA
TTAAATATAGTAAAGATACTAGATATAACGATAACATTGATAAATCATTTTTAGTTACCC
ATGACAAACAAAATTATCAAGCTTTCCCATGTTCAATTTTAGAAGATGTTAAAGAACAAG
CACAAAACTATGATGTAATTGGTATTGATGAAGGCCAATTTTTCCCAGACGTTGTACAAT
TTAGTGAAGACTTGGCAAATCAAGGTAAAACCGTTATAATTNCCGCACTAGATGGTACCT
TCCAAAGAAAACCATTCCAATCAGTCATTGATTTAGTATCAAAGGCTGAATATATTACAA
AGTTAACTGCCGTTTGTATGGTTTGNTACAATGAAGCCGCCTTTTCAAAACGTATCGTCG
AAAGTGATGATATCGAATTAATTGGTGGTATAGNANAAATAATTTCCGTT
sequence update 2001.11.24
Translated Amino Acid sequence 
iythsht*sylyiyflinkksyiylinllsyklkplllnnlhhinlyf*yifftliffys
pfkkfkfkliin*n*IQKMIVTQIAGKIQVIFGPMFSGKTTELIRRIKRFNFANKKCLLI
KYSKDTRYNDNIDKSFLVTHDKQNYQAFPCSILEDVKEQAQNYDVIGIDEGQFFPDVVQF
SEDLANQGKTVIIXALDGTFQRKPFQSVIDLVSKAEYITKLTAVCMVXYNEAAFSKRIVE
SDDIELIGGIXXIISV
Translated Amino Acid sequence (All Frames) 
Frame A:
tyththtlnhiyiyif*liknhiyil*icfhtn*nryy*ityii*iytfsisfslssffi
phlknsnln**likikfkk*l*lkllvkfksslvqclaerplssleelkdlilqtknay*
lnivkildititlinhf*lpmtnkiiklshvqf*kmlknkhktmm*lvlmkanfsqtlyn
lvktwqikvkpl*xph*mvpskenhsnqsli*yqrlnilqs*lpfvwfxtmkppfqnvss
kvmisn*lvv*xk*fp


Frame B:
hihtlthliifiyiffn**kiiyisykfafiqiktviik*ltsykfillvylfhshlflf
pi*kiqi*idn*lklnskndcnsncw*nsshlwsnv*rkdh*ah*kn*ki*fckqkmlin
*i**ry*i*r*h**iifsyp*qtklssfpmfnfrrc*rtstkl*cnwy**rpifprrcti
**rlgksr*nrynxrtrwylpkktipish*fsikg*iyykvncrlyglxq*srlfktyrr
k**yrinwwyxxnnfr


Frame C:
iythsht*sylyiyflinkksyiylinllsyklkplllnnlhhinlyf*yifftliffys
pfkkfkfkliin*n*IQKMIVTQIAGKIQVIFGPMFSGKTTELIRRIKRFNFANKKCLLI
KYSKDTRYNDNIDKSFLVTHDKQNYQAFPCSILEDVKEQAQNYDVIGIDEGQFFPDVVQF
SEDLANQGKTVIIXALDGTFQRKPFQSVIDLVSKAEYITKLTAVCMVXYNEAAFSKRIVE
SDDIELIGGIXXIISV
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFI730 (VFI730Q) /CSM/VF/VFI7-B/VFI730Q.Seq.d/                       1031   0.0
VSJ693 (VSJ693Q) /CSM/VS/VSJ6-D/VSJ693Q.Seq.d/                       1009   0.0
VHQ109 (VHQ109Q) /CSM/VH/VHQ1-A/VHQ109Q.Seq.d/                       1009   0.0
VFL501 (VFL501Q) /CSM/VF/VFL5-A/VFL501Q.Seq.d/                       1009   0.0
VFC738 (VFC738Q) /CSM/VF/VFC7-B/VFC738Q.Seq.d/                       1009   0.0
SFI673 (SFI673Q) /CSM/SF/SFI6-D/SFI673Q.Seq.d/                       1009   0.0
SFE794 (SFE794Q) /CSM/SF/SFE7-D/SFE794Q.Seq.d/                       1009   0.0
VFL617 (VFL617Q) /CSM/VF/VFL6-A/VFL617Q.Seq.d/                       1003   0.0
VFK621 (VFK621Q) /CSM/VF/VFK6-A/VFK621Q.Seq.d/                       1003   0.0
VFE258 (VFE258Q) /CSM/VF/VFE2-C/VFE258Q.Seq.d/                       1003   0.0
own update 2003. 6.19
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(U61990) Dictyostelium discoideum ThyB (thyB) mRNA, complete...   954   0.0    2
(AF510846) Dictyostelium discoideum calmodulin-binding prote...   954   0.0    2
(AU062155) Dictyostelium discoideum slug cDNA, clone SLH838.      841   0.0    3
(BJ432335) Dictyostelium discoideum cDNA clone:ddv18l07, 3' ...   969   0.0    1
(AY192984) Dictyostelium discoideum thymidine kinase 1 (TK1)...   954   0.0    1
(AU270331) Dictyostelium discoideum vegetative cDNA clone:VS...   954   0.0    1
(BJ446521) Dictyostelium discoideum cDNA clone:ddv63a03, 3' ...   954   0.0    1
(BJ409200) Dictyostelium discoideum cDNA clone:dds39l24, 3' ...   954   0.0    1
(BJ434474) Dictyostelium discoideum cDNA clone:ddv24a01, 3' ...   948   0.0    1
(BJ432463) Dictyostelium discoideum cDNA clone:ddv18c21, 3' ...   948   0.0    1
dna update 2009. 1. 8
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P04183) RecName: Full=Thymidine kinase, cytosolic;          EC=...   187   2e-46
AY335565_1(AY335565|pid:none) Synthetic construct Homo sapiens t...   187   2e-46
BT030694_1(BT030694|pid:none) Bos taurus thymidine kinase 1, sol...   187   4e-46
K02581_1(K02581|pid:none) Human thymidine kinase mRNA, complete ...   186   5e-46
AB451268_1(AB451268|pid:none) Homo sapiens TK1 mRNA for thymidin...   186   7e-46
AM905441_1(AM905441|pid:none) Suberites domuncula mRNA for thymi...   185   1e-45
(P09768) RecName: Full=Thymidine kinase, cytosolic;          EC=...   184   3e-45
(P68563) RecName: Full=Thymidine kinase;          EC=2.7.1.21;  ...   183   5e-45
(P04184) RecName: Full=Thymidine kinase, cytosolic;          EC=...   183   6e-45
AF163844_1(AF163844|pid:none) Cowpox virus thymidine kinase gene...   182   8e-45
protein update 2009. 7.11
PSORT 

 psg: 0.88  gvh: 0.34  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for VFI730 is cyt
5' end seq. ID VFI730F
5' end seq. 
>VFI730F.Seq
ACATATACACACACTCACACACTTAATCATATTTATATATATATTTTTTAATTAATAAAA
AATCATATATATATCTTATAAATTTGCTTTCATACAAATTAAAACCGTTATTATTAAATA
ACTTACATCATATAAATTTATACTTTTAGTATATCTTTTTCACTCTCATCTTTTTTTATT
CCCCATTTAAAAAATTCAAATTTAAATTGATAATTAATTAAAATTAAATTCAAAAAATGA
TTGTAACTCAAATTGCTGGTAAAATTCAAGTCATCTTTGGTCCAATGTTTAGCGGAAAGA
CCACTGAGCTCATTAGAAGAATTAAAAGATTTAATTTTGCAAACAAAAAATGCTTATTAA
TTAAATATAGTAAAGATACTAGATATAACGATAACATTGATAAATCATTTTTAGTTACCC
ATGACAAACAAAATTATCAAGCTTTCCCATGTTCAATTTTAGAAGATGTTAAAGAACAAG
CACAAAACTATGATGTAATTGGTATTGATGAAGGCCAATTTTTCCCAGACGTTGTACAAT
TTAGTGAAGACTTGGCAAATCAAGGTAAAACCGTNNNNNNNNNN
Length of 5' end seq. 584
3' end seq. ID VFI730Z
3' end seq. 
>VFI730Z.Seq
NNNNNNNNNNGTAAAATTCAAGTCATCTTTGGTCCAATGTTTAGCGGAAAGACCACTGAG
CTCATTAGAAGAATTAAAAGATTTAATTTTGCAAACAAAAAATGCTTATTAATTAAATAT
AGTAAAGATACTAGATATANCGATAACATTGATAAATCATTTTTAGTTACCCATGACAAA
CAAAATTATCAAGCTTTCCCATGTTCAATTTTAGAAGATGTTAAAGAACAAGCACAAAAC
TATGATGTAATTGGTATTGATGAAGGCCAATTTTTCCCAGACGTTGTACAATTTAGTGAA
GACTTGGCAAATCAAGGTAAAACCGTTATAATTNCCGCACTAGATGGTACCTTCCAAAGA
AAACCATTCCAATCAGTCATTGATTTAGTATCAAAGGCTGAATATATTACAAAGTTAACT
GCCGTTTGTATGGTTTGNTACAATGAAGCCGCCTTTTCAAAACGTATCGTCGAAAGTGAT
GATATCGAATTAATTGGTGGTATAGNANAAATAATTTCCGTT
Length of 3' end seq. 522
Connected seq. ID VFI730P
Connected seq. 
>VFI730P.Seq
ACATATACACACACTCACACACTTAATCATATTTATATATATATTTTTTAATTAATAAAA
AATCATATATATATCTTATAAATTTGCTTTCATACAAATTAAAACCGTTATTATTAAATA
ACTTACATCATATAAATTTATACTTTTAGTATATCTTTTTCACTCTCATCTTTTTTTATT
CCCCATTTAAAAAATTCAAATTTAAATTGATAATTAATTAAAATTAAATTCAAAAAATGA
TTGTAACTCAAATTGCTGGTAAAATTCAAGTCATCTTTGGTCCAATGTTTAGCGGAAAGA
CCACTGAGCTCATTAGAAGAATTAAAAGATTTAATTTTGCAAACAAAAAATGCTTATTAA
TTAAATATAGTAAAGATACTAGATATAACGATAACATTGATAAATCATTTTTAGTTACCC
ATGACAAACAAAATTATCAAGCTTTCCCATGTTCAATTTTAGAAGATGTTAAAGAACAAG
CACAAAACTATGATGTAATTGGTATTGATGAAGGCCAATTTTTCCCAGACGTTGTACAAT
TTAGTGAAGACTTGGCAAATCAAGGTAAAACCGT----------GTAAAATTCAAGTCAT
CTTTGGTCCAATGTTTAGCGGAAAGACCACTGAGCTCATTAGAAGAATTAAAAGATTTAA
TTTTGCAAACAAAAAATGCTTATTAATTAAATATAGTAAAGATACTAGATATANCGATAA
CATTGATAAATCATTTTTAGTTACCCATGACAAACAAAATTATCAAGCTTTCCCATGTTC
AATTTTAGAAGATGTTAAAGAACAAGCACAAAACTATGATGTAATTGGTATTGATGAAGG
CCAATTTTTCCCAGACGTTGTACAATTTAGTGAAGACTTGGCAAATCAAGGTAAAACCGT
TATAATTNCCGCACTAGATGGTACCTTCCAAAGAAAACCATTCCAATCAGTCATTGATTT
AGTATCAAAGGCTGAATATATTACAAAGTTAACTGCCGTTTGTATGGTTTGNTACAATGA
AGCCGCCTTTTCAAAACGTATCGTCGAAAGTGATGATATCGAATTAATTGGTGGTATAGN
ANAAATAATTTCCGTT
Length of connected seq. 1086
Full length Seq ID VFI730E
Full length Seq. 
>VFI730E.Seq
ACATATACACACACTCACACACTTAATCATATTTATATATATATTTTTTAATTAATAAAA
AATCATATATATATCTTATAAATTTGCTTTCATACAAATTAAAACCGTTATTATTAAATA
ACTTACATCATATAAATTTATACTTTTAGTATATCTTTTTCACTCTCATCTTTTTTTATT
CCCCATTTAAAAAATTCAAATTTAAATTGATAATTAATTAAAATTAAATTCAAAAAATGA
TTGTAACTCAAATTGCTGGTAAAATTCAAGTCATCTTTGGTCCAATGTTTAGCGGAAAGA
CCACTGAGCTCATTAGAAGAATTAAAAGATTTAATTTTGCAAACAAAAAATGCTTATTAA
TTAAATATAGTAAAGATACTAGATATAACGATAACATTGATAAATCATTTTTAGTTACCC
ATGACAAACAAAATTATCAAGCTTTCCCATGTTCAATTTTAGAAGATGTTAAAGAACAAG
CACAAAACTATGATGTAATTGGTATTGATGAAGGCCAATTTTTCCCAGACGTTGTACAAT
TTAGTGAAGACTTGGCAAATCAAGGTAAAACCGTTATAATTNCCGCACTAGATGGTACCT
TCCAAAGAAAACCATTCCAATCAGTCATTGATTTAGTATCAAAGGCTGAATATATTACAA
AGTTAACTGCCGTTTGTATGGTTTGNTACAATGAAGCCGCCTTTTCAAAACGTATCGTCG
AAAGTGATGATATCGAATTAATTGGTGGTATAGNANAAATAATTTCCGTT
Length of full length seq. 770