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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFI680
Library VF
(Link to library)
Clone ID VFI680
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VFI680P
(Link to Original site)
Representative DNA sequence 
>VFI680 (VFI680Q) /CSM/VF/VFI6-D/VFI680Q.Seq.d/
TGGCCTACTGGCTTAAAACAAATAAATTAAATTAAATAAAAAATAAAAATGGATGGTGAA
GATGTTCAAGCTTTAGTTATTGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGT
GACGATGCTCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTT
ATGGTTGGTATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGT
ATCTTAACCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAA
AAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCACCAGAAGAACACCCAGTT
CTCTTAACCGAAGCTCCATTAAATCCAAAAGCCAACAGAGAAAAAATGACCCAAATTATG
TTTGAAACCTTCAACACCCCAGCCATGTACGTTGCCATTCAAGCCGTCTTATCCTTATAT
GCCTCTGGTCGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTA
CCAATCTATGAAGGTTATGCCTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGT
GATCTCACCGATTACATGATGAAAATCTTAACTGAXXXXXXXXXXTNCAACCATCATTNT
TAGGTATGGAATNTGCTGGTATCCNCGAAACCACATTCAANTCCNTCATGAAATGTGATG
TTNANATCCGTAAAAATTTANACGGTAANNTNGTCNTANCAGGTGGNACAACTATGTTCC
CAGGTATTGCTGATTGTATNAACAAANAATTAANTGNTTTAGCNCCNTCNNCCATGAAAA
TTAAAATCNTTGCTCCNCCAGAACGTAAANACTCTGTCTGGATTGGTGGATCNANTTTAG
NTTCNCTCNCAACNTTCCAANAAATNTGGATCNCNAAANAANAATAT
sequence update 2001.11.22
Translated Amino Acid sequence 
wptglkqin*ik*KIKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGV
MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPV
LLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVSHTV
PIYEGYALPHAILRLDLAGRDLTDYMMKILT---

---QPSXLGMEXAGIXETTFXSXMKCDVXIRKNLXGXXVXXGGTTMFPGIADCXNKXLXX
LAPSXMKIKIXAPPERKXSVWIGGSXLXSLXTFQXXWIXKXXY
Translated Amino Acid sequence (All Frames) 
Frame A:
wptglkqin*ik*KIKMDGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGV
MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPV
LLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVSHTV
PIYEGYALPHAILRLDLAGRDLTDYMMKILT---

---xnhhx*vwnxlvsxkphsxps*nvmxxsvkixtvxxsxqvxqlcsqvllivxtxn*x
x*xxxp*klksllxqnvxtlsglvdxx*xxsqxsxkxgsxxxn

Frame B:
glla*nk*iklnkk*kwmvkmfkl*llitvlvcvkpvllvtmlhvlfshqllvvqdtlvl
wlvwvkkthm*vmkpnqrevs*psntqlntvslptgmiwkksgiilstmnsvlhqkntqf
s*pklh*iqkptekk*pklclkpstpqpctlpfkpsypymplvvppvllwiqvmvsptly
qsmkvmpyhmpsyv*i*lvvispit**ks*l---

---xtiixrygxcwyprnhiqxxhem*cxxp*kfxr*xxxxrwxnyvpryc*lyxqxixx
fsxxxhen*nxcsxrt*xlcldwwixfxfxxnxpxnxdxxxxi

Frame C:
aywlktnkln*iknkngw*rcssfsy**rfwyv*srfcw*rcstccfpincwsskthwcy
gwygskrlicr**spikerylnpqipn*tryryqlg*ygknlasyflq*tpcctrrtpss
lnrssiksksqqrkndpnyv*nlqhpshvrchssrliliclwsyhrycygfr*wclphct
nl*rlclttchltfrfsws*shrlhdenln*---

---QPSXLGMEXAGIXETTFXSXMKCDVXIRKNLXGXXVXXGGTTMFPGIADCXNKXLXX
LAPSXMKIKIXAPPERKXSVWIGGSXLXSLXTFQXXWIXKXXY
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFI680 (VFI680Q) /CSM/VF/VFI6-D/VFI680Q.Seq.d/                       1495   0.0
VFL353 (VFL353Q) /CSM/VF/VFL3-C/VFL353Q.Seq.d/                       1162   0.0
VFL311 (VFL311Q) /CSM/VF/VFL3-A/VFL311Q.Seq.d/                       1162   0.0
VFJ294 (VFJ294Q) /CSM/VF/VFJ2-D/VFJ294Q.Seq.d/                       1162   0.0
SLH494 (SLH494Q) /CSM/SL/SLH4-D/SLH494Q.Seq.d/                       1162   0.0
SLH344 (SLH344Q) /CSM/SL/SLH3-B/SLH344Q.Seq.d/                       1162   0.0
SLG493 (SLG493Q) /CSM/SL/SLG4-D/SLG493Q.Seq.d/                       1162   0.0
SLG440 (SLG440Q) /CSM/SL/SLG4-B/SLG440Q.Seq.d/                       1162   0.0
SLE330 (SLE330Q) /CSM/SL/SLE3-B/SLE330Q.Seq.d/                       1162   0.0
SLE295 (SLE295Q) /CSM/SL/SLE2-D/SLE295Q.Seq.d/                       1162   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(DL073163) Expression method of recombinant protein having t...  1160   0.0    1
(DL073162) Expression method of recombinant protein having t...  1160   0.0    1
(M14146) D.discoideum actin 15 gene, complete cds.               1160   0.0    1
(AC115680) Dictyostelium discoideum chromosome 2 map 4915084...  1160   0.0    1
(AU062081) Dictyostelium discoideum slug cDNA, clone SLH494.     1160   0.0    1
(AU062044) Dictyostelium discoideum slug cDNA, clone SLH344.     1160   0.0    1
(AU061891) Dictyostelium discoideum slug cDNA, clone SLG493.     1160   0.0    1
(AU061876) Dictyostelium discoideum slug cDNA, clone SLG440.     1160   0.0    1
(AU061458) Dictyostelium discoideum slug cDNA, clone SLE330.     1160   0.0    1
(AU061449) Dictyostelium discoideum slug cDNA, clone SLE295.     1160   0.0    1
dna update 2009. 1. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A03000(A03000)actin 3 - fruit fly (Drosophila melanogaster) (fra...   317   e-117
(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   400   e-110
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   400   e-110
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   398   e-109
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   397   e-109
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        394   e-108
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   392   e-108
AY294152_1(AY294152|pid:none) Mayorella sp. JJP-2003 actin mRNA,...   392   e-107
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   390   e-107
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          390   e-107
protein update 2009. 6.21
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.14  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VFI680 is cyt
5' end seq. ID VFI680F
5' end seq. 
>VFI680F.Seq
TGGCCTACTGGCTTAAAACAAATAAATTAAATTAAATAAAAAATAAAAATGGATGGTGAA
GATGTTCAAGCTTTAGTTATTGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGT
GACGATGCTCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTT
ATGGTTGGTATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGT
ATCTTAACCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAA
AAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCACCAGAAGAACACCCAGTT
CTCTTAACCGAAGCTCCATTAAATCCAAAAGCCAACAGAGAAAAAATGACCCAAATTATG
TTTGAAACCTTCAACACCCCAGCCATGTACGTTGCCATTCAAGCCGTCTTATCCTTATAT
GCCTCTGGTCGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTA
CCAATCTATGAAGGTTATGCCTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGT
GATCTCACCGATTACATGATGAAAATCTTAACTGANNNNNNNNNN
Length of 5' end seq. 645
3' end seq. ID VFI680Z
3' end seq. 
>VFI680Z.Seq
NNNNNNNNNNTNCAACCATCATTNTTAGGTATGGAATNTGCTGGTATCCNCGAAACCACA
TTCAANTCCNTCATGAAATGTGATGTTNANATCCGTAAAAATTTANACGGTAANNTNGTC
NTANCAGGTGGNACAACTATGTTCCCAGGTATTGCTGATTGTATNAACAAANAATTAANT
GNTTTAGCNCCNTCNNCCATGAAAATTAAAATCNTTGCTCCNCCAGAACGTAAANACTCT
GTCTGGATTGGTGGATCNANTTTAGNTTCNCTCNCAACNTTCCAANAAATNTGGATCNCN
AAANAANAATAT
Length of 3' end seq. 312
Connected seq. ID VFI680P
Connected seq. 
>VFI680P.Seq
TGGCCTACTGGCTTAAAACAAATAAATTAAATTAAATAAAAAATAAAAATGGATGGTGAA
GATGTTCAAGCTTTAGTTATTGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGT
GACGATGCTCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTT
ATGGTTGGTATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGT
ATCTTAACCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAA
AAAATCTGGCATCATACTTTCTACAATGAACTCCGTGTTGCACCAGAAGAACACCCAGTT
CTCTTAACCGAAGCTCCATTAAATCCAAAAGCCAACAGAGAAAAAATGACCCAAATTATG
TTTGAAACCTTCAACACCCCAGCCATGTACGTTGCCATTCAAGCCGTCTTATCCTTATAT
GCCTCTGGTCGTACCACCGGTATTGTTATGGATTCAGGTGATGGTGTCTCCCACACTGTA
CCAATCTATGAAGGTTATGCCTTACCACATGCCATCTTACGTTTAGATTTAGCTGGTCGT
GATCTCACCGATTACATGATGAAAATCTTAACTGA----------TNCAACCATCATTNT
TAGGTATGGAATNTGCTGGTATCCNCGAAACCACATTCAANTCCNTCATGAAATGTGATG
TTNANATCCGTAAAAATTTANACGGTAANNTNGTCNTANCAGGTGGNACAACTATGTTCC
CAGGTATTGCTGATTGTATNAACAAANAATTAANTGNTTTAGCNCCNTCNNCCATGAAAA
TTAAAATCNTTGCTCCNCCAGAACGTAAANACTCTGTCTGGATTGGTGGATCNANTTTAG
NTTCNCTCNCAACNTTCCAANAAATNTGGATCNCNAAANAANAATAT
Length of connected seq. 937
Full length Seq ID -
Full length Seq. -
Length of full length seq. -