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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFI451
Library VF
(Link to library)
Clone ID VFI451
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15456-1
Original site URL
Representative seq. ID VFI451P
(Link to Original site)
Representative DNA sequence 
>VFI451 (VFI451Q) /CSM/VF/VFI4-C/VFI451Q.Seq.d/
ACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACTATT
GTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCTAGA
AGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACCACAATC
CCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCAGGT
CAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAGAAA
TGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCAAGC
ACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCAGGT
TTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTATTCGCT
GAAGCCACTGCCGAACCAXXXXXXXXXXTAATTCAATTCCACCAGTCTTTTACAACATGT
TATCACAAGGTTTAGTTTCATCAACACTCTTCTCCTTCTGGTTATCAAGAACTCCAGGTG
CCAACGGTGGTGAACTCTCATTCGGTTCAATCGATAACACTAAATACACTGGTGACATTA
CCTACGTCCCATTAACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTATCG
ATGGTCAATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTACAT
CACTCATTGCTGGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGTCA
TCTTAAATGGTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAACACCTTACCAAATGTTA
CCATCACCGTTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGAATACGTTTTAGAAGTTA
CTGAGTTCGGAAAGACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGGAA
ATTTCTGGATCCTTGGTGATGTTTTCATCTCTGCTTACTATACTGTATTCGATTTTGGTA
ATAAACAAGTTGGTTTCGCAACTGCCATTCAAGGTTAAATTTTTTTAATCTAATTTATA
sequence update 2001.11.22
Translated Amino Acid sequence 
t*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGTTI
PISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGASS
TYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAEATAEP---

---NSIPPVFYNMLSQGLVSSTLFSFWLSRTPGANGGELSFGSIDNTKYTGDITYVPLTN
ETYWEFVMDDFAIDGQSAGFCGTTCHAICDSGTSLIAGPMADITALNEKLGAVILNGEGV
FSDCSVINTLPNVTITVAGREFVLTPKEYVLEVTEFGKTECLSGFMGIELNMGNFWILGD
VFISAYYTVFDFGNKQVGFATAIQG*ifli*fi
Translated Amino Acid sequence (All Frames) 
Frame A:
t*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGTTI
PISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGASS
TYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAEATAEP---

---*fnstsllqhvitrfsfintllllviknsrcqrw*tlirfnr*h*ihw*hylrpinq
rnllgiryg*lcyrwsiswflwyylsrnlrfryithcwsng*yycpq*kircchlkw*rc
ll*l*ryqhltkcyhhrcws*icfnskrirfrsy*vrkd*mfewiygyrvkhgkfldpw*
cfhlcllycirfw**tswfrnchsrlnffnliy

Frame B:
pdlhkrk*nylf*lyf*lllf*lkl*qyh*tsiklqenleeefhkngqtdyllsmlvpqs
qfqilkmlntmvplplvpqvkpsk*fsilvhptcgfhqrnvqslllhviyitnitavpqa
hmsptelispsntvvvlcqvlslkipsllvh*llkinyslkplpn---

---NSIPPVFYNMLSQGLVSSTLFSFWLSRTPGANGGELSFGSIDNTKYTGDITYVPLTN
ETYWEFVMDDFAIDGQSAGFCGTTCHAICDSGTSLIAGPMADITALNEKLGAVILNGEGV
FSDCSVINTLPNVTITVAGREFVLTPKEYVLEVTEFGKTECLSGFMGIELNMGNFWILGD
VFISAYYTVFDFGNKQVGFATAIQG*ifli*fi

Frame C:
ltyikeneityfnfifsyycfsssfnstiklpssfkri*kksstkmvkqiicsqcwyhnp
nfrf*rcsilwchyhwyprsslqssfrywfiqlvdsikemsnhcccm*ft*qi*qrclkh
icrqrn*fhhpir*wcyvrfclsrfrhcwfinc*rsiir*shcrt---

---iqfhqsfttcyhkv*fhqhsspsgyqelqvptvvnshsvqsitlntlvtlptsh*pt
kpignslwmtllsmvnqlvsvvllvtqfaiqvhhsllvqwlillpsmkn*vlss*mvkvs
slivalstpyqmlpspllvvnlf*lqkntf*kllsserlnv*vdlwvss*tweisgslvm
fsslltilysilvinklvsqlpfkvkff*snl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFI451 (VFI451Q) /CSM/VF/VFI4-C/VFI451Q.Seq.d/                       2159   0.0
VFN650 (VFN650Q) /CSM/VF/VFN6-C/VFN650Q.Seq.d/                       1203   0.0
VFN427 (VFN427Q) /CSM/VF/VFN4-B/VFN427Q.Seq.d/                       1203   0.0
VFN380 (VFN380Q) /CSM/VF/VFN3-D/VFN380Q.Seq.d/                       1203   0.0
VFN333 (VFN333Q) /CSM/VF/VFN3-B/VFN333Q.Seq.d/                       1203   0.0
VFN222 (VFN222Q) /CSM/VF/VFN2-A/VFN222Q.Seq.d/                       1203   0.0
VFM881 (VFM881Q) /CSM/VF/VFM8-D/VFM881Q.Seq.d/                       1203   0.0
VFM767 (VFM767Q) /CSM/VF/VFM7-C/VFM767Q.Seq.d/                       1203   0.0
VFM519 (VFM519Q) /CSM/VF/VFM5-A/VFM519Q.Seq.d/                       1203   0.0
VFM401 (VFM401Q) /CSM/VF/VFM4-A/VFM401Q.Seq.d/                       1203   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ432082) Dictyostelium discoideum cDNA clone:ddv17f14, 3' ...  1211   0.0    1
(Y16962) Dictyostelium discoideum mRNA for cathepsin D.          1203   0.0    1
(AJ243946) Dictyostelium discoideum ctsD gene for cathepsin ...  1203   0.0    1
(AU039684) Dictyostelium discoideum slug cDNA, clone SLG179.     1203   0.0    1
(AU039614) Dictyostelium discoideum slug cDNA, clone SLE842.     1203   0.0    1
(AU034144) Dictyostelium discoideum slug cDNA, clone SLB793.     1203   0.0    1
(BJ435814) Dictyostelium discoideum cDNA clone:ddv28c14, 3' ...  1203   0.0    1
(BJ435701) Dictyostelium discoideum cDNA clone:ddv27p19, 3' ...  1203   0.0    1
(BJ435614) Dictyostelium discoideum cDNA clone:ddv27n14, 3' ...  1203   0.0    1
(BJ435576) Dictyostelium discoideum cDNA clone:ddv27f18, 3' ...  1203   0.0    1
dna update 2008.12.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q03168) RecName: Full=Lysosomal aspartic protease;          EC=...   204   4e-90
EF213114_1(EF213114|pid:none) Penaeus monodon cathepsin D mRNA, ...   197   5e-89
BT080419_1(BT080419|pid:none) Caligus clemensi clone ccle-evs-50...   209   6e-89
EF070454_1(EF070454|pid:none) Maconellicoccus hirsutus clone WHM...   199   1e-88
DQ010007_1(DQ010007|pid:none) Bombyx mori CathD mRNA, complete c...   193   2e-88
AB106552_1(AB106552|pid:none) Todarodes pacificus tpaD mRNA for ...   188   9e-87
DQ909010_1(DQ909010|pid:none) Clonorchis sinensis aspartic prote...   199   6e-86
FJ654712_1(FJ654712|pid:none) Chrysomela tremulae aspartic prote...   183   3e-85
AF454831_1(AF454831|pid:none) Apriona germari cathepsin D mRNA, ...   181   1e-84
FJ168036_1(FJ168036|pid:none) Fasciola hepatica cathepsin D-like...   194   2e-84
protein update 2009. 6.21
PSORT 

 psg: 0.98  gvh: 0.77  alm: 0.42  top: 0.57  tms: 0.00  mit: 0.36  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: extracellular, including cell wall
	 16.0 %: mitochondrial
	 16.0 %: nuclear
	 12.0 %: vacuolar
	  8.0 %: cytoplasmic
	  4.0 %: cytoskeletal

>> prediction for VFI451 is exc
5' end seq. ID VFI451F
5' end seq. 
>VFI451F.Seq
ACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACTATT
GTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCTAGA
AGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACCACAATC
CCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCAGGT
CAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAGAAA
TGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCAAGC
ACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCAGGT
TTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTATTCGCT
GAAGCCACTGCCGAACCANNNNNNNNNN
Length of 5' end seq. 508
3' end seq. ID VFI451Z
3' end seq. 
>VFI451Z.Seq
NNNNNNNNNNTAATTCAATTCCACCAGTCTTTTACAACATGTTATCACAAGGTTTAGTTT
CATCAACACTCTTCTCCTTCTGGTTATCAAGAACTCCAGGTGCCAACGGTGGTGAACTCT
CATTCGGTTCAATCGATAACACTAAATACACTGGTGACATTACCTACGTCCCATTAACCA
ACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTT
TCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGGTCCAA
TGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTG
TCTTCTCTGATTGTAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTC
GTGAATTTGTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTG
AATGTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATCCTTGGTG
ATGTTTTCATCTCTGCTTACTATACTGTATTCGATTTTGGTAATAAACAAGTTGGTTTCG
CAACTGCCATTCAAGGTTAAATTTTTTTAATCTAATTTATA
Length of 3' end seq. 641
Connected seq. ID VFI451P
Connected seq. 
>VFI451P.Seq
ACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACTATT
GTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCTAGA
AGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACCACAATC
CCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCAGGT
CAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAGAAA
TGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCAAGC
ACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCAGGT
TTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTATTCGCT
GAAGCCACTGCCGAACCA----------TAATTCAATTCCACCAGTCTTTTACAACATGT
TATCACAAGGTTTAGTTTCATCAACACTCTTCTCCTTCTGGTTATCAAGAACTCCAGGTG
CCAACGGTGGTGAACTCTCATTCGGTTCAATCGATAACACTAAATACACTGGTGACATTA
CCTACGTCCCATTAACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTATCG
ATGGTCAATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTACAT
CACTCATTGCTGGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGTCA
TCTTAAATGGTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAACACCTTACCAAATGTTA
CCATCACCGTTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGAATACGTTTTAGAAGTTA
CTGAGTTCGGAAAGACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGGAA
ATTTCTGGATCCTTGGTGATGTTTTCATCTCTGCTTACTATACTGTATTCGATTTTGGTA
ATAAACAAGTTGGTTTCGCAACTGCCATTCAAGGTTAAATTTTTTTAATCTAATTTATA
Length of connected seq. 1129
Full length Seq ID -
Full length Seq. -
Length of full length seq. -