>VFI186 (VFI186Q) /CSM/VF/VFI1-D/VFI186Q.Seq.d/
ATATATACACCAAAAATGAATTGGAAAGAGCAACTTGAAGAAATTGGTAATTGCCAAGAT
TCAAACAAAGCTATCCAAGATTATAATAAAATTCTCACTATTCAAGAATCACCAGATGAT
ATTAAAGAGGAAGCAATTCTTAGATTAGCAAAATTATTTGTAAAGATTGGTAAAGGTGAT
CAATTACCAACACTTTTAAGATCAGTTAGACCATTTTTTGATAAAATTTCTAAACCAAAG
ACTGATAAAATCGTTAGAAATTTTATTGATATTTTCTCAACCGTACCTGATAATTTAACA
ACTTTAATTGAATTTGTCAAAGAGAATATTCAATGGTGTAAAGATACAAATAGAATTTAT
TTAAGACAAAGATTAGAAACTAAACTTTTTACATTAATGTTTGAAGCAAAAGATTATGCA
AATGCATTAAGCGGATTAACAACATTATTAACAGAAATTAAAAGATTGGATGATAAACCA
TTATTAGTTGAAATTCAACTTGTTGAGAGTAGAATTCAACATGCTCTTAAAAATATTCCA
AAAGCAAGAGCTGCTTTAACTTCTGCTCGTACAAATGCAAATACAATCTATTXXXXXXXX
XXAAACTTCAAGCTGAAATCGATATGCAATCTGGTATTTTACATTCTGAAGAAAAAGATT
ATAAAACTGCTTTTTCATACTTTTTCGAATCATATGAAACTTATGATAGTCTTGAAGATC
CATTTGCAATGAAAGCATTAAAGTATATGTTACTTTGTAAAATTATGACCAACCAAACTG
ATGATGTTCATGCATTAGTTAATGGTAAAATTGGTTTGAAATATCATAACAATAGATCCA
TTGAAGCAATTACTCAAATTTCAAAATCTCATGCAAAGAGATCACTTCATATGTTCCAAG
AGGTAACCAAAGAATTTTCTGATCAATTATCTGATGATCCAATCATTCATAGTCATTTAA
CTGAATTATATAGTAATCTTCTTGAACAAAATTTATGTCGTATTATTGAACCATTCTCAA
GGGTTGAAATTTCACATATTGCAAAATTAATTGATTTACCAGTTGATGTTGTTGAAAGAA
AATTATCACTTATGATCCTTGATAAGAAATATAATGGTATCCTCGATCAAGGTACTGGTA
CTTTAATTGTATTTGAAGAACAAAAAGAAGATAAATTATTTAATTGTTCTTTAGAAACTA
TTGGTGCTTTAAGTCGTGTTGTNATTTACTTTANAAAAAACAAACAAACTTTCTTAAATT

IYTPKMNWKEQLEEIGNCQDSNKAIQDYNKILTIQESPDDIKEEAILRLAKLFVKIGKGD
QLPTLLRSVRPFFDKISKPKTDKIVRNFIDIFSTVPDNLTTLIEFVKENIQWCKDTNRIY
LRQRLETKLFTLMFEAKDYANALSGLTTLLTEIKRLDDKPLLVEIQLVESRIQHALKNIP
KARAALTSARTNANTIY---

---KLQAEIDMQSGILHSEEKDYKTAFSYFFESYETYDSLEDPFAMKALKYMLLCKIMTN
QTDDVHALVNGKIGLKYHNNRSIEAITQISKSHAKRSLHMFQEVTKEFSDQLSDDPIIHS
HLTELYSNLLEQNLCRIIEPFSRVEISHIAKLIDLPVDVVERKLSLMILDKKYNGILDQG
TGTLIVFEEQKEDKLFNCSLETIGALSRVVIYFXKNKQTFLN

Frame A:
IYTPKMNWKEQLEEIGNCQDSNKAIQDYNKILTIQESPDDIKEEAILRLAKLFVKIGKGD
QLPTLLRSVRPFFDKISKPKTDKIVRNFIDIFSTVPDNLTTLIEFVKENIQWCKDTNRIY
LRQRLETKLFTLMFEAKDYANALSGLTTLLTEIKRLDDKPLLVEIQLVESRIQHALKNIP
KARAALTSARTNANTIY---

---KLQAEIDMQSGILHSEEKDYKTAFSYFFESYETYDSLEDPFAMKALKYMLLCKIMTN
QTDDVHALVNGKIGLKYHNNRSIEAITQISKSHAKRSLHMFQEVTKEFSDQLSDDPIIHS
HLTELYSNLLEQNLCRIIEPFSRVEISHIAKLIDLPVDVVERKLSLMILDKKYNGILDQG
TGTLIVFEEQKEDKLFNCSLETIGALSRVVIYFXKNKQTFLN

Frame B:
yihqk*igksnlkklviakiqtklskiiikfslfknhqmilkrkqfld*qnyl*rlvkvi
nyqhf*dqldhflikflnqrliksleillifsqpylii*ql*lnlskrifngvkiqiefi
*dkd*klnflh*clkqkimqmh*ad*qhy*qklkdwminhy*lkfnllrvefnmllkifq
kqell*lllvqmqiqsi---

---nfklksicnlvfyilkkkiikllfhtfsnhmklmivlkihlq*kh*sicyfvkl*pt
klmmfmh*lmvklv*niitidplkqllkfqnlmqrdhficskr*pknflinylmiqsfiv
i*lnyiviflnkiyvvllnhsqglkfhilqn*liyqlmllkenyhl*slirnimvssikv
lvl*lylknkkkinylivl*kllvl*vvlxftlxktnkls*i

Frame C:
iytknelerat*rnw*lprfkqsyprl**nshysritr*y*rgsns*iskiickdw*r*s
itntfkis*tif**nf*tkd**nr*kfy*yflnrt**fnnfn*icqreysmv*ryk*nlf
ktkirn*tfyinv*skrlckcikrinniinrn*kig**tiis*nstc*e*nstcs*kysk
skscfnfcsykckynl---

---tss*nryaiwyftf*rkrl*ncffilfrii*nl**s*rsicnesikvyvtl*nydqp
n**cscis*w*nwfeis*q*ih*snysnfkisckeitsyvprgnqrif*sii**snhs*s
fn*ii**ss*tkfmsyy*tilkg*nftyckin*fts*cc*kkiitydp**ei*wyprsry
wyfnci*rtkrr*ii*lffrnywcfksccxllxkkqtnflk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFI186 (VFI186Q) /CSM/VF/VFI1-D/VFI186Q.Seq.d/                       2385   0.0
AFK194 (AFK194Q) /CSM/AF/AFK1-D/AFK194Q.Seq.d/                       2369   0.0
AFG786 (AFG786Q) /CSM/AF/AFG7-D/AFG786Q.Seq.d/                       2361   0.0
AFD866 (AFD866Q) /CSM/AF/AFD8-C/AFD866Q.Seq.d/                       2359   0.0
AFK213 (AFK213Q) /CSM/AF/AFK2-A/AFK213Q.Seq.d/                       2355   0.0
VFC823 (VFC823Q) /CSM/VF/VFC8-A/VFC823Q.Seq.d/                       2353   0.0
AFG380 (AFG380Q) /CSM/AF/AFG3-D/AFG380Q.Seq.d/                       2349   0.0
AFB850 (AFB850Q) /CSM/AF/AFB8-C/AFB850Q.Seq.d/                       1304   0.0
VFK702 (VFK702Q) /CSM/VF/VFK7-A/VFK702Q.Seq.d/                       1302   0.0
AFM681 (AFM681Q) /CSM/AF/AFM6-D/AFM681Q.Seq.d/                       1287   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AX488960|AX488960.1 Sequence 6260 from Patent WO02053728.          74   4e-18  3
AJ497077|AJ497077.1 Medicago truncatula EST, clone mt--abc955104c04.    68   5e-10  3
CB893586|CB893586.1 EST646378 HOGA Medicago truncatula cDNA clone HOGA-28K10, mRNA sequence.    68   7e-10  3
CG960238|CG960238.1 MBEKP41TF mth2 Medicago truncatula genomic clone 75H10, genomic survey sequence.    68   9e-10  3
AP004173|AP004173.1 Mycoplasma penetrans genomic DNA, complete sequence, section 4/5.    34   2e-09  22
BE320243|BE320243.2 NF024E04RT1F1023 Developing root Medicago truncatula cDNA clone NF024E04RT 5', mRNA sequence.    68   6e-08  2
AL408021|AL408021.1 T7 end of clone AV0AA006B01 of library AV0AA from strain CBS 379 of Saccharomyces exiguus.    52   9e-08  4
AW126253|AW126253.2 N100360e rootphos(-) Medicago truncatula cDNA clone MHRP-5A2, mRNA sequence.    68   1e-07  2
CG936521|CG936521.1 MBEIO22TF mth2 Medicago truncatula genomic clone 63D19, genomic survey sequence.    68   1e-07  2
BG648576|BG648576.1 EST510195 HOGA Medicago truncatula cDNA clone pHOGA-23M13 5' end, mRNA sequence.    68   1e-07  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54UB5) RecName: Full=26S proteasome non-ATPase regulatory subu...   410   0.0
AM424416_1(AM424416|pid:none) Vitis vinifera contig VV78X196128....   251   e-101
BT053774_1(BT053774|pid:none) Zea mays full-length cDNA clone ZM...   247   e-100
AY230834_1(AY230834|pid:none) Arabidopsis thaliana 26S proteasom...   245   e-100
EU969379_1(EU969379|pid:none) Zea mays clone 329471 26S proteaso...   246   e-100
BT069707_1(BT069707|pid:none) Zea mays full-length cDNA clone ZM...   246   e-99
A86414(A86414) hypothetical protein F28N24.15 - Arabidopsis thal...   245   e-99
AY230837_1(AY230837|pid:none) Arabidopsis thaliana 26S proteasom...   245   1e-99
AK064788_1(AK064788|pid:none) Oryza sativa Japonica Group cDNA c...   246   2e-99
BT035753_1(BT035753|pid:none) Zea mays full-length cDNA clone ZM...   244   1e-98

 psg: 0.58  gvh: 0.35  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VFI186 is cyt

>VFI186F.Seq
ATATATACACCAAAAATGAATTGGAAAGAGCAACTTGAAGAAATTGGTAATTGCCAAGAT
TCAAACAAAGCTATCCAAGATTATAATAAAATTCTCACTATTCAAGAATCACCAGATGAT
ATTAAAGAGGAAGCAATTCTTAGATTAGCAAAATTATTTGTAAAGATTGGTAAAGGTGAT
CAATTACCAACACTTTTAAGATCAGTTAGACCATTTTTTGATAAAATTTCTAAACCAAAG
ACTGATAAAATCGTTAGAAATTTTATTGATATTTTCTCAACCGTACCTGATAATTTAACA
ACTTTAATTGAATTTGTCAAAGAGAATATTCAATGGTGTAAAGATACAAATAGAATTTAT
TTAAGACAAAGATTAGAAACTAAACTTTTTACATTAATGTTTGAAGCAAAAGATTATGCA
AATGCATTAAGCGGATTAACAACATTATTAACAGAAATTAAAAGATTGGATGATAAACCA
TTATTAGTTGAAATTCAACTTGTTGAGAGTAGAATTCAACATGCTCTTAAAAATATTCCA
AAAGCAAGAGCTGCTTTAACTTCTGCTCGTACAAATGCAAATACAATCTATTNNNNNNNN
NN

>VFI186Z.Seq
NNNNNNNNNNAAACTTCAAGCTGAAATCGATATGCAATCTGGTATTTTACATTCTGAAGA
AAAAGATTATAAAACTGCTTTTTCATACTTTTTCGAATCATATGAAACTTATGATAGTCT
TGAAGATCCATTTGCAATGAAAGCATTAAAGTATATGTTACTTTGTAAAATTATGACCAA
CCAAACTGATGATGTTCATGCATTAGTTAATGGTAAAATTGGTTTGAAATATCATAACAA
TAGATCCATTGAAGCAATTACTCAAATTTCAAAATCTCATGCAAAGAGATCACTTCATAT
GTTCCAAGAGGTAACCAAAGAATTTTCTGATCAATTATCTGATGATCCAATCATTCATAG
TCATTTAACTGAATTATATAGTAATCTTCTTGAACAAAATTTATGTCGTATTATTGAACC
ATTCTCAAGGGTTGAAATTTCACATATTGCAAAATTAATTGATTTACCAGTTGATGTTGT
TGAAAGAAAATTATCACTTATGATCCTTGATAAGAAATATAATGGTATCCTCGATCAAGG
TACTGGTACTTTAATTGTATTTGAAGAACAAAAAGAAGATAAATTATTTAATTGTTCTTT
AGAAACTATTGGTGCTTTAAGTCGTGTTGTNATTTACTTTANAAAAAACAAACAAACTTT
CTTAAATT

>VFI186P.Seq
ATATATACACCAAAAATGAATTGGAAAGAGCAACTTGAAGAAATTGGTAATTGCCAAGAT
TCAAACAAAGCTATCCAAGATTATAATAAAATTCTCACTATTCAAGAATCACCAGATGAT
ATTAAAGAGGAAGCAATTCTTAGATTAGCAAAATTATTTGTAAAGATTGGTAAAGGTGAT
CAATTACCAACACTTTTAAGATCAGTTAGACCATTTTTTGATAAAATTTCTAAACCAAAG
ACTGATAAAATCGTTAGAAATTTTATTGATATTTTCTCAACCGTACCTGATAATTTAACA
ACTTTAATTGAATTTGTCAAAGAGAATATTCAATGGTGTAAAGATACAAATAGAATTTAT
TTAAGACAAAGATTAGAAACTAAACTTTTTACATTAATGTTTGAAGCAAAAGATTATGCA
AATGCATTAAGCGGATTAACAACATTATTAACAGAAATTAAAAGATTGGATGATAAACCA
TTATTAGTTGAAATTCAACTTGTTGAGAGTAGAATTCAACATGCTCTTAAAAATATTCCA
AAAGCAAGAGCTGCTTTAACTTCTGCTCGTACAAATGCAAATACAATCTATT--------
--AAACTTCAAGCTGAAATCGATATGCAATCTGGTATTTTACATTCTGAAGAAAAAGATT
ATAAAACTGCTTTTTCATACTTTTTCGAATCATATGAAACTTATGATAGTCTTGAAGATC
CATTTGCAATGAAAGCATTAAAGTATATGTTACTTTGTAAAATTATGACCAACCAAACTG
ATGATGTTCATGCATTAGTTAATGGTAAAATTGGTTTGAAATATCATAACAATAGATCCA
TTGAAGCAATTACTCAAATTTCAAAATCTCATGCAAAGAGATCACTTCATATGTTCCAAG
AGGTAACCAAAGAATTTTCTGATCAATTATCTGATGATCCAATCATTCATAGTCATTTAA
CTGAATTATATAGTAATCTTCTTGAACAAAATTTATGTCGTATTATTGAACCATTCTCAA
GGGTTGAAATTTCACATATTGCAAAATTAATTGATTTACCAGTTGATGTTGTTGAAAGAA
AATTATCACTTATGATCCTTGATAAGAAATATAATGGTATCCTCGATCAAGGTACTGGTA
CTTTAATTGTATTTGAAGAACAAAAAGAAGATAAATTATTTAATTGTTCTTTAGAAACTA
TTGGTGCTTTAAGTCGTGTTGTNATTTACTTTANAAAAAACAAACAAACTTTCTTAAATT