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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFI173
Library VF
(Link to library)
Clone ID VFI173
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15323-1
Original site URL
Representative seq. ID VFI173P
(Link to Original site)
Representative DNA sequence 
>VFI173 (VFI173Q) /CSM/VF/VFI1-D/VFI173Q.Seq.d/
AATATTTTATATTGCACACCATTATACTATATACTTTTTTTTTTAAAATGAATTCTAAAT
TTGCTGATGAATTAATTGCAACTGCTAAAGCAATTGTAGCACCAGGTAAAGGTATTTTAG
CAGCAGATGAATCAACCAATACAATTGGAGCTCGTTTCAAAAAGATTAATTTAGAAAACA
ATGAAGAGAACAGAAGAGCCTACAGAGAACTTTTAATTGGTACTGGTAATGGTGTTAACG
AATTTATTGGTGGTATTATTTTATATGAAGAAACTNTTTACCAAAAGATGGCAGATGGTA
GATTATTCACAGATGTTTTAAGAGAACAAAAGATTCACATTGGTATTAAAGTTGATAAGG
GTGTTGTACCAATTCCAGGTACCGATGGTGAAACCTCAACCCAAGGTTTAGATGGTCTTG
CAGAGCGTTGTAAGAAATACTATGAAGCAGGTGCACGTTTCGCAAAATGGAGAGCAGTCT
TAAAGATTGATCTTGCCAAGAATTGTCCATCCCAATTATCAATCACTGAAAATGCACACA
CATTAGCTCGTTATGCTGCAATTTGTXXXXXXXXXXGGTGAATCTGCCAACGTTGAAGCT
GCTCGTAAAGTTTATTTACATCGTGCCAAGTGTAACTCTCTTGCTCAACTCGGTAAATAC
ACTGGTGAGGAATCAACTGGTTCAGCATCTGAATCATTATATGTAAAAGATTACAAATAT
TAAATATATAAAATAA
sequence update 2001.11.22
Translated Amino Acid sequence 
YFILHTIILYTFFFKMNSKFADELIATAKAIVAPGKGILAADESTNTIGARFKKINLENN
EENRRAYRELLIGTGNGVNEFIGGIILYEETXYQKMADGRLFTDVLREQKIHIGIKVDKG
VVPIPGTDGETSTQGLDGLAERCKKYYEAGARFAKWRAVLKIDLAKNCPSQLSITENAHT
LARYAAIC---

---GESANVEAARKVYLHRAKCNSLAQLGKYTGEESTGSASESLYVKDYKY*iyki
Translated Amino Acid sequence (All Frames) 
Frame A:
nilyctplyyilfflk*ilnllmn*lqllkql*hqvkvf*qqmnqpiqlelvskrli*kt
mkrteeptenf*lvlvmvltnllvvlfymkklftkrwqmvdysqmf*enkrftlvlklir
vlyqfqvpmvkpqpkv*mvlqsvvrntmkqvhvsqngeqs*rlilprivhpnyqslkmht
h*lvmlqf---

---GESANVEAARKVYLHRAKCNSLAQLGKYTGEESTGSASESLYVKDYKY*iyki

Frame B:
ifyiahhytiyfff*nef*ic**incnc*sncstr*ryfssr*inqynwssfqkd*frkq
*reqkslqrtfnwyw*wc*riywwyyfi*rnxlpkdgrw*iihrcfkrtkdshwy*s**g
cctnsryrw*nlnprfrwscral*eil*srctfrkmesslkd*scqelsipiinh*kcth
isslccnl---

---vnlptlkllvkfiyivpsvtlllnsvntlvrnqlvqhlnhym*kitnikyik*

Frame C:
YFILHTIILYTFFFKMNSKFADELIATAKAIVAPGKGILAADESTNTIGARFKKINLENN
EENRRAYRELLIGTGNGVNEFIGGIILYEETXYQKMADGRLFTDVLREQKIHIGIKVDKG
VVPIPGTDGETSTQGLDGLAERCKKYYEAGARFAKWRAVLKIDLAKNCPSQLSITENAHT
LARYAAIC---

---*icqr*scs*slftscqv*lscstr*ihw*ginwfsi*iiickrlqilni*n
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFI173 (VFI173Q) /CSM/VF/VFI1-D/VFI173Q.Seq.d/                       1334   0.0
VFM802 (VFM802Q) /CSM/VF/VFM8-A/VFM802Q.Seq.d/                       1057   0.0
VFM614 (VFM614Q) /CSM/VF/VFM6-A/VFM614Q.Seq.d/                       1057   0.0
VFK562 (VFK562Q) /CSM/VF/VFK5-C/VFK562Q.Seq.d/                       1051   0.0
CFF463 (CFF463Q) /CSM/CF/CFF4-C/CFF463Q.Seq.d/                       1051   0.0
AFN510 (AFN510Q) /CSM/AF/AFN5-A/AFN510Q.Seq.d/                       1051   0.0
AFJ144 (AFJ144Q) /CSM/AF/AFJ1-B/AFJ144Q.Seq.d/                       1051   0.0
CFJ531 (CFJ531Q) /CSM/CF/CFJ5-B/CFJ531Q.Seq.d/                       1047   0.0
VFI422 (VFI422Q) /CSM/VF/VFI4-A/VFI422Q.Seq.d/                       1045   0.0
SFF123 (SFF123Q) /CSM/SF/SFF1-A/SFF123Q.Seq.d/                       1045   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   946   0.0    10
AF247061|AF247061.1 Plasmodium vinckei fructose 1,6-bisphosphate aldolase gene, partial cds.    54   2e-15  3
AF217804|AF217804.1 Cyanidium caldarium fructose-1,6-biphosphate aldolase (Aldo) mRNA, complete cds.    52   2e-14  4
AF247060|AF247060.1 Plasmodium chabaudi AS fructose 1,6-bisphosphate aldolase gene, partial cds.    54   1e-12  3
BM163016|BM163016.1 EST565539 PyBS Plasmodium yoelii yoelii cDNA clone PYCKZ83 5' end, mRNA sequence.    46   3e-11  3
BM161478|BM161478.1 EST564001 PyBS Plasmodium yoelii yoelii cDNA clone PYCKF30 5' end, mRNA sequence.    46   3e-11  3
BM167314|BM167314.1 EST569837 PyBS Plasmodium yoelii yoelii cDNA clone PYCOH24 5' end, mRNA sequence.    46   3e-11  3
AF247059|AF247059.1 Plasmodium yoelii YM fructose 1,6-bisphosphate aldolase gene, partial cds.    46   7e-11  3
BG409414|BG409414.1 00786 leafy spurge Lambda HybriZAP 2.1 two-hybrid vector cDNA Library Euphorbia esula cDNA clone 25AD 5' similar to fructose bisphosphate aldolase, mRNA sequence.    70   5e-08  1
AF247062|AF247062.1 Plasmodium berghei QIMR fructose 1,6-bisphosphate aldolase gene, partial cds.    46   7e-07  3
dna update 2004. 1.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86A67) RecName: Full=Fructose-bisphosphate aldolase;          ...   305   7e-82
AF217804_1(AF217804|pid:none) Cyanidium caldarium fructose-1,6-b...   189   8e-47
AL162506_11(AL162506|pid:none) Arabidopsis thaliana DNA chromoso...   188   1e-46
AY062566_1(AY062566|pid:none) Arabidopsis thaliana fructose-bisp...   188   1e-46
AF308587_1(AF308587|pid:none) Fragaria x ananassa cytosolic aldo...   187   3e-46
DQ294273_1(DQ294273|pid:none) Solanum tuberosum clone 156H06 fru...   183   4e-45
DQ222486_1(DQ222486|pid:none) Solanum tuberosum clone 097F12 unk...   183   4e-45
DQ235169_1(DQ235169|pid:none) Solanum tuberosum clone 154D06 fru...   183   4e-45
DQ228325_1(DQ228325|pid:none) Solanum tuberosum clone 135C01 unk...   181   2e-44
EF144942_1(EF144942|pid:none) Populus trichocarpa clone WS0111_B...   181   3e-44
protein update 2009. 6.21
PSORT 

 psg: 0.97  gvh: 0.40  alm: 0.39  top: 0.50  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for VFI173 is cyt
5' end seq. ID VFI173F
5' end seq. 
>VFI173F.Seq
AATATTTTATATTGCACACCATTATACTATATACTTTTTTTTTTAAAATGAATTCTAAAT
TTGCTGATGAATTAATTGCAACTGCTAAAGCAATTGTAGCACCAGGTAAAGGTATTTTAG
CAGCAGATGAATCAACCAATACAATTGGAGCTCGTTTCAAAAAGATTAATTTAGAAAACA
ATGAAGAGAACAGAAGAGCCTACAGAGAACTTTTAATTGGTACTGGTAATGGTGTTAACG
AATTTATTGGTGGTATTATTTTATATGAAGAAACTNTTTACCAAAAGATGGCAGATGGTA
GATTATTCACAGATGTTTTAAGAGAACAAAAGATTCACATTGGTATTAAAGTTGATAAGG
GTGTTGTACCAATTCCAGGTACCGATGGTGAAACCTCAACCCAAGGTTTAGATGGTCTTG
CAGAGCGTTGTAAGAAATACTATGAAGCAGGTGCACGTTTCGCAAAATGGAGAGCAGTCT
TAAAGATTGATCTTGCCAAGAATTGTCCATCCCAATTATCAATCACTGAAAATGCACACA
CATTAGCTCGTTATGCTGCAATTTGTNNNNNNNNNN
Length of 5' end seq. 576
3' end seq. ID VFI173Z
3' end seq. 
>VFI173Z.Seq
NNNNNNNNNNGGTGAATCTGCCAACGTTGAAGCTGCTCGTAAAGTTTATTTACATCGTGC
CAAGTGTAACTCTCTTGCTCAACTCGGTAAATACACTGGTGAGGAATCAACTGGTTCAGC
ATCTGAATCATTATATGTAAAAGATTACAAATATTAAATATATAAAATAA
Length of 3' end seq. 170
Connected seq. ID VFI173P
Connected seq. 
>VFI173P.Seq
AATATTTTATATTGCACACCATTATACTATATACTTTTTTTTTTAAAATGAATTCTAAAT
TTGCTGATGAATTAATTGCAACTGCTAAAGCAATTGTAGCACCAGGTAAAGGTATTTTAG
CAGCAGATGAATCAACCAATACAATTGGAGCTCGTTTCAAAAAGATTAATTTAGAAAACA
ATGAAGAGAACAGAAGAGCCTACAGAGAACTTTTAATTGGTACTGGTAATGGTGTTAACG
AATTTATTGGTGGTATTATTTTATATGAAGAAACTNTTTACCAAAAGATGGCAGATGGTA
GATTATTCACAGATGTTTTAAGAGAACAAAAGATTCACATTGGTATTAAAGTTGATAAGG
GTGTTGTACCAATTCCAGGTACCGATGGTGAAACCTCAACCCAAGGTTTAGATGGTCTTG
CAGAGCGTTGTAAGAAATACTATGAAGCAGGTGCACGTTTCGCAAAATGGAGAGCAGTCT
TAAAGATTGATCTTGCCAAGAATTGTCCATCCCAATTATCAATCACTGAAAATGCACACA
CATTAGCTCGTTATGCTGCAATTTGT----------GGTGAATCTGCCAACGTTGAAGCT
GCTCGTAAAGTTTATTTACATCGTGCCAAGTGTAACTCTCTTGCTCAACTCGGTAAATAC
ACTGGTGAGGAATCAACTGGTTCAGCATCTGAATCATTATATGTAAAAGATTACAAATAT
TAAATATATAAAATAA
Length of connected seq. 726
Full length Seq ID -
Full length Seq. -
Length of full length seq. -