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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFI169
Library VF
(Link to library)
Clone ID VFI169
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12232-1
Original site URL
Representative seq. ID VFI169P
(Link to Original site)
Representative DNA sequence 
>VFI169 (VFI169Q) /CSM/VF/VFI1-C/VFI169Q.Seq.d/
ATACAACAATATCCAATTATATTTTTAATCCTAAAAAAAACCCAACAGTTTTATTTAGAA
ACTCTTTTAAAACCTTAAAGTTTGAATTTTTTTTTTTTTTAAAAAAAAATCTNTTTTTTT
ATTTTTTAAAATTTTNATTTTTNCATATNTTTTTTTTTCAATTNTTTTTTTTTNNNAAXX
XXXXXXXXGCCGAAAGAAATTACCAAAAAATGAGATTAATTGCTGCTCTTTTATCTGTAT
TATTAATAGCTTCAACTGCTCAATCAACTGTTACAATTTATGAAAGTTCAAAACCAGGTG
ATTTTGACTTTTATTTATTTGTTCAACAATGGATTTATTCATACTGCGATTCACAAACTT
GTATTCAAAATAAAGAAAGAGAAGCATTCACTATCCATGGTTTATGGCCAGAGAATAGTG
ATGGCAGTTATCCATCATTCTGTTCAGGTCCATCATTCAATGTTAATGCTATCCAAGATT
TAGAAGATCAATTAAACTTTGATTGGCCATCACTCACTGGTCCAAACACTGATTTCTGGA
CTCACGAATTCAGCAAACATGGTACTTGTTCAATCACAGGTCCAATCACAGATATTCACG
ATTATTTCGCTACTGGTATCAAACTTTACACCGAATTCAATATCACTGCTGCCCTCGAAT
CTGAAAATATTTATCCATCAGATAGCAACACTTACAAACCAGTCGATATCACTAATGCTA
TCACCACTCATTTTGGTGGTAAACCAGGTATTCAATGTTCATCTGGTCAATTATCAACTG
TTGCTGTTTGTATCGATAAAAATAGTTTATCA
sequence update 2001.11.22
Translated Amino Acid sequence 
TTISNYIFNPKKNPTVLFRNSFKTLKFEFFFFLKKNLFFYFLKFXFXHXFFFQXFFFX--
-

---AERNYQKMRLIAALLSVLLIASTAQSTVTIYESSKPGDFDFYLFVQQWIYSYCDSQT
CIQNKEREAFTIHGLWPENSDGSYPSFCSGPSFNVNAIQDLEDQLNFDWPSLTGPNTDFW
THEFSKHGTCSITGPITDIHDYFATGIKLYTEFNITAALESENIYPSDSNTYKPVDITNA
ITTHFGGKPGIQCSSGQLSTVAVCIDKNSLS
Translated Amino Acid sequence (All Frames) 
Frame A:
iqqypiiflilkktqqfyletllkp*slnffff*kkixffif*nfxfxixfffnxffxx-
--

---AERNYQKMRLIAALLSVLLIASTAQSTVTIYESSKPGDFDFYLFVQQWIYSYCDSQT
CIQNKEREAFTIHGLWPENSDGSYPSFCSGPSFNVNAIQDLEDQLNFDWPSLTGPNTDFW
THEFSKHGTCSITGPITDIHDYFATGIKLYTEFNITAALESENIYPSDSNTYKPVDITNA
ITTHFGGKPGIQCSSGQLSTVAVCIDKNSLS

Frame B:
ynniqlyf*s*kkpnsfi*klf*nlkv*iffffkkksxflffkixifxyxffsixffxx-
--

---pkeitkk*d*lllfylyy**lqllnqllqfmkvqnqviltfiylfnngfihtaihkl
vfkikkekhslsmvygqrivmavihhsvqvhhsmlmlski*kin*tlighhslvqtlisg
ltnsanmvlvqsqvqsqiftiisllvsnftpnsisllpsnlkifihqiatltnqsislml
splilvvnqvfnvhlvnyqlllfvsikivy

Frame C:
TTISNYIFNPKKNPTVLFRNSFKTLKFEFFFFLKKNLFFYFLKFXFXHXFFFQXFFFX--
-

---rkklpkneinccsficiinsfncsincynl*kfktr*f*llficstmdlfilrftnl
ysk*rkrsihypwfmare**wqlsiilfrsiiqc*cyprfrrsikl*laithwskh*fld
sriqqtwylfnhrsnhrysrlfrywyqtlhriqyhccpri*kylsir*qhlqtsryh*cy
hhsfww*trysmfiwsiinccclyr*k*fi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHB567 (VHB567Q) /CSM/VH/VHB5-C/VHB567Q.Seq.d/                       1237   0.0
VFI169 (VFI169Q) /CSM/VF/VFI1-C/VFI169Q.Seq.d/                       1237   0.0
SSI141 (SSI141Q) /CSM/SS/SSI1-B/SSI141Q.Seq.d/                       1237   0.0
VFH722 (VFH722Q) /CSM/VF/VFH7-A/VFH722Q.Seq.d/                       1183   0.0
VFL529 (VFL529Q) /CSM/VF/VFL5-B/VFL529Q.Seq.d/                       1116   0.0
SSF854 (SSF854Q) /CSM/SS/SSF8-C/SSF854Q.Seq.d/                       1088   0.0
VFL650 (VFL650Q) /CSM/VF/VFL6-C/VFL650Q.Seq.d/                       1057   0.0
SSA623 (SSA623Q) /CSM/SS/SSA6-A/SSA623Q.Seq.d/                       1035   0.0
VSA582 (VSA582Q) /CSM/VS/VSA5-D/VSA582Q.Seq.d/                        934   0.0
SSB204 (SSB204Q) /CSM/SS/SSB2-A/SSB204Q.Seq.d/                        876   0.0
own update 2002.12.18
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115680|AC115680.2 Dictyostelium discoideum chromosome 2 map 4415041-4520361 strain AX4, complete sequence.    62   3e-26  11
AC024930|AC024930.4 Homo sapiens chromosome 15 clone RP11-228M15, WORKING DRAFT SEQUENCE, 24 unordered pieces.    40   0.013  6
AC128817|AC128817.3 Rattus norvegicus clone CH230-47N24, *** SEQUENCING IN PROGRESS ***, 18 unordered pieces.    38   0.065  7
AC147483|AC147483.5 Medicago truncatula clone mth2-22o7, WORKING DRAFT SEQUENCE, 11 unordered pieces.    34   0.14   6
AC005140|AC005140.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    32   0.15   13
AC073087|AC073087.4 Homo sapiens chromosome 1 clone RP11-317F13, WORKING DRAFT SEQUENCE, 12 unordered pieces.    48   0.19   1
AL024509|AL024509.1 Human DNA sequence from clone 522P13 on chromosome 6p21.31-22.3.  Contains a 60S Ribosomal Protein L21 pseudogene and an HNRNP A3  (Heterogenous Nuclear Riboprotein A3, FBRNP) pseudogene. Contains  ESTs, STSs and GSSs.    48   0.19   1
AP003192|AP003192.2 Clostridium perfringens str. 13 DNA, complete genome, section 8/10.    36   0.20   10
AL442643|AL442643.2 Human DNA sequence from clone RP11-564H19 on chromosome 13 Contains  STSs and GSSs.    32   0.39   9
Z98551|Z98551.1 Plasmodium falciparum MAL3P6.                      34   0.39   7
dna update 2004. 1.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q7M438) RecName: Full=Ribonuclease DdI;          Short=RNase Dd...   394   e-108
JE0316(JE0316;JE0407)protozoan RNase,DdI - slime mold (Dictyoste...   379   e-104
AB034248_1(AB034248|pid:none) Volvox carteri vrn1 mRNA for S-lik...   137   3e-31
U13256_1(U13256|pid:none) Nicotiana alata RNase NE mRNA, complet...   130   4e-29
(P80022) RecName: Full=Extracellular ribonuclease LE;          S...   130   5e-29
AC146971_5(AC146971|pid:none) Medicago truncatula clone mth2-128...   129   1e-28
JC5518(JC5518)ribonuclease M5 (EC 3.1.-.-) precursor, wound-indu...   128   2e-28
AB034638_1(AB034638|pid:none) Nicotiana tabacum mRNA for RNase, ...   128   2e-28
AB112028_1(AB112028|pid:none) Nicotiana glutinosa ngr1 gene for ...   128   2e-28
S66342(S66342) ribonuclease II (EC 3.1.-.-) precursor, wound-ind...   127   4e-28
protein update 2009. 6.21
PSORT 

 psg: 0.59  gvh: 0.49  alm: 0.29  top: 0.27  tms: 0.07  mit: 0.39  mip: 0.12
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: mitochondrial
	 16.0 %: nuclear
	 16.0 %: endoplasmic reticulum
	 12.0 %: Golgi
	  4.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VFI169 is cyt
5' end seq. ID VFI169F
5' end seq. 
>VFI169F.Seq
ATACAACAATATCCAATTATATTTTTAATCCTAAAAAAAACCCAACAGTTTTATTTAGAA
ACTCTTTTAAAACCTTAAAGTTTGAATTTTTTTTTTTTTTAAAAAAAAATCTNTTTTTTT
ATTTTTTAAAATTTTNATTTTTNCATATNTTTTTTTTTCAATTNTTTTTTTTTNNNAANN
NNNNNNNN
Length of 5' end seq. 188
3' end seq. ID VFI169Z
3' end seq. 
>VFI169Z.Seq
NNNNNNNNNNGCCGAAAGAAATTACCAAAAAATGAGATTAATTGCTGCTCTTTTATCTGT
ATTATTAATAGCTTCAACTGCTCAATCAACTGTTACAATTTATGAAAGTTCAAAACCAGG
TGATTTTGACTTTTATTTATTTGTTCAACAATGGATTTATTCATACTGCGATTCACAAAC
TTGTATTCAAAATAAAGAAAGAGAAGCATTCACTATCCATGGTTTATGGCCAGAGAATAG
TGATGGCAGTTATCCATCATTCTGTTCAGGTCCATCATTCAATGTTAATGCTATCCAAGA
TTTAGAAGATCAATTAAACTTTGATTGGCCATCACTCACTGGTCCAAACACTGATTTCTG
GACTCACGAATTCAGCAAACATGGTACTTGTTCAATCACAGGTCCAATCACAGATATTCA
CGATTATTTCGCTACTGGTATCAAACTTTACACCGAATTCAATATCACTGCTGCCCTCGA
ATCTGAAAATATTTATCCATCAGATAGCAACACTTACAAACCAGTCGATATCACTAATGC
TATCACCACTCATTTTGGTGGTAAACCAGGTATTCAATGTTCATCTGGTCAATTATCAAC
TGTTGCTGTTTGTATCGATAAAAATAGTTTATCA
Length of 3' end seq. 634
Connected seq. ID VFI169P
Connected seq. 
>VFI169P.Seq
ATACAACAATATCCAATTATATTTTTAATCCTAAAAAAAACCCAACAGTTTTATTTAGAA
ACTCTTTTAAAACCTTAAAGTTTGAATTTTTTTTTTTTTTAAAAAAAAATCTNTTTTTTT
ATTTTTTAAAATTTTNATTTTTNCATATNTTTTTTTTTCAATTNTTTTTTTTTNNNAA--
--------GCCGAAAGAAATTACCAAAAAATGAGATTAATTGCTGCTCTTTTATCTGTAT
TATTAATAGCTTCAACTGCTCAATCAACTGTTACAATTTATGAAAGTTCAAAACCAGGTG
ATTTTGACTTTTATTTATTTGTTCAACAATGGATTTATTCATACTGCGATTCACAAACTT
GTATTCAAAATAAAGAAAGAGAAGCATTCACTATCCATGGTTTATGGCCAGAGAATAGTG
ATGGCAGTTATCCATCATTCTGTTCAGGTCCATCATTCAATGTTAATGCTATCCAAGATT
TAGAAGATCAATTAAACTTTGATTGGCCATCACTCACTGGTCCAAACACTGATTTCTGGA
CTCACGAATTCAGCAAACATGGTACTTGTTCAATCACAGGTCCAATCACAGATATTCACG
ATTATTTCGCTACTGGTATCAAACTTTACACCGAATTCAATATCACTGCTGCCCTCGAAT
CTGAAAATATTTATCCATCAGATAGCAACACTTACAAACCAGTCGATATCACTAATGCTA
TCACCACTCATTTTGGTGGTAAACCAGGTATTCAATGTTCATCTGGTCAATTATCAACTG
TTGCTGTTTGTATCGATAAAAATAGTTTATCA
Length of connected seq. 802
Full length Seq ID -
Full length Seq. -
Length of full length seq. -