>VFI139 (VFI139Q) /CSM/VF/VFI1-B/VFI139Q.Seq.d/
AATTATATATATAGAACATCCTTTCTAAATCGAAATAACTTTAATTAATTAGGTTTTAGT
TTATACAATATATAGGGAGAAAATATATAATACAGAATGAGTCTTGTACAAAAAATCAAA
GAGGTCGAGGATGAGATGGCTCGTACCCAAAAAAATAAAGCTACAAGCTTTCATTTAGGT
GTATTGAAAGCAAAATTAGCAAAATATAAAAGAGAATTATTGTTAGGACCAAGTAAAGGT
GCAGCAGCAGGTGCAGGTGAAGGTTTCGATGTATCAAAAGCAGGTGATGCTCGTGTTGGT
TTAATTGGTTTCCCATCAGTTGGTAAATCAACTTTATTAACTAAATTAACTGGTACCTCA
TCTGAAGTTGCCTCATACGAATTCACAACTTTAACATGTATTCCTGGTGTAATTAATTAT
AAAGGTGCTAAAATTCAATTATTAGATTTACCAGGTATTATTGAAGGTGCAAAAGATGGT
AAAGGTAGAGGTAGACAAGTTATTGCAGTTGGTAGAACATGTAATCTTATTTTAATTGTA
TTGGATGCAATGAAACCATTGGTACATAAAAAGATTATAGAGAGAGAGTTGGATGGTTTT
XXXXXXXXXXCCACCACCAATCACATTCAAAAAGAAGGAGAAGGGTGGTATTAACTTTTC
ACATACACCAAATGTCAATCCAACCCAATTGGATAGTGAAACTGTGAAAGCAATCTGTGC
AGAATATAAAATTCACAATGCAGATGTGATTTTACGTGGAAATTGTACAGTGGATGAATT
CATCGATGTCATTGAAGGCAATCGTATCTACGTTCCATGTATTTACGTTTTAAATAAGAT
CGATGCTATCTCTATCGAAGAGTTGGACCTTTTAGATAAAATTCCTCATTATGTTCCAAT
CTCTTCTCATTTGGAATGGAATTTAGATGCCCTCCTCGATAAAATTTGGGAATACTTAAA
ATTGATTAGAGTTTACACTAAACCAAAAGGTCTCATTCCAGATTACAATGAACCAGTCGT
CATTAGAGGTGGTGAAGAAGCTTCAATTGAAACTTTTTGTAATCATATTCATAATTCAAT
TATTAGACAATTTAGATATGCTTTGGTTTGGGGTTCTTCAGCTAAACATAACCCTCAACG
TTGTGGTAAGGATCACGTTTTAGCAGATGAAGATATTGTTCAAATTGTTAAAAAATAAGA
TCACATTTTAATGGNAAAGATAA

nyiyrtsflnrnnfn*lgfslyni*geni*YRMSLVQKIKEVEDEMARTQKNKATSFHLG
VLKAKLAKYKRELLLGPSKGAAAGAGEGFDVSKAGDARVGLIGFPSVGKSTLLTKLTGTS
SEVASYEFTTLTCIPGVINYKGAKIQLLDLPGIIEGAKDGKGRGRQVIAVGRTCNLILIV
LDAMKPLVHKKIIERELDGF---

---PPPITFKKKEKGGINFSHTPNVNPTQLDSETVKAICAEYKIHNADVILRGNCTVDEF
IDVIEGNRIYVPCIYVLNKIDAISIEELDLLDKIPHYVPISSHLEWNLDALLDKIWEYLK
LIRVYTKPKGLIPDYNEPVVIRGGEEASIETFCNHIHNSIIRQFRYALVWGSSAKHNPQR
CGKDHVLADEDIVQIVKK*dhilmxki

Frame A:
nyiyrtsflnrnnfn*lgfslyni*geni*YRMSLVQKIKEVEDEMARTQKNKATSFHLG
VLKAKLAKYKRELLLGPSKGAAAGAGEGFDVSKAGDARVGLIGFPSVGKSTLLTKLTGTS
SEVASYEFTTLTCIPGVINYKGAKIQLLDLPGIIEGAKDGKGRGRQVIAVGRTCNLILIV
LDAMKPLVHKKIIERELDGF---

---PPPITFKKKEKGGINFSHTPNVNPTQLDSETVKAICAEYKIHNADVILRGNCTVDEF
IDVIEGNRIYVPCIYVLNKIDAISIEELDLLDKIPHYVPISSHLEWNLDALLDKIWEYLK
LIRVYTKPKGLIPDYNEPVVIRGGEEASIETFCNHIHNSIIRQFRYALVWGSSAKHNPQR
CGKDHVLADEDIVQIVKK*dhilmxki

Frame B:
iiyiehpf*ieitlin*vlvytiyrekiynte*vlykkskrsrmrwlvpkkiklqafi*v
y*kqn*qnikenyc*dqvkvqqqvqvkvsmyqkqvmlvlv*lvshqlvnqly*ln*lvph
lklphtnsql*hvflv*liikvlkfny*iyqvllkvqkmvkvevdkllqlvehvilf*ly
wmq*nhwyikrl*reswmv---

---hhqshskrrrrvvltfhihqmsiqpnwivkl*kqsvqnikftmqm*fyveivqwmns
smslkaivstfhvftf*irsmlslskswtf*ikflimfqslliwngi*mpssikfgnt*n
*leftlnqkvsfqitmnqsslevvkklqlklfviifiiqlldnldmlwfgvlqlnitlnv
vvritf*qmkilfkllknkitf*wxr*

Frame C:
lyi*nilsksk*l*lirf*fiqyigrkyiiqnesctknqrgrg*dgsypkk*syklsfrc
ieskiski*kriivrtk*rcssrcr*rfrciksr*cscwfnwfpisw*infin*inwyli
*sclirihnfnmyswcn*l*rc*nsiirftryy*rckrw*r*r*tsycsw*nm*syfnci
gcnetigt*kdyrervgwf---

---ttnhiqkegegwy*lftytkcqsnpig**ncesnlcri*nsqcrcdftwklysg*ih
rch*rqsylrsmylrfk*drcylyrrvgpfr*nsslcsnlfsfgmefrcppr*nlgilki
d*slh*tkrshsrlq*tsrh*rw*rsfn*nfl*sys*fny*ti*icfglgffs*t*pstl
w*gsrfsr*rycsnc*kirshfngkd

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFI139 (VFI139Q) /CSM/VF/VFI1-B/VFI139Q.Seq.d/                       2317   0.0
VFK836 (VFK836Q) /CSM/VF/VFK8-B/VFK836Q.Seq.d/                       1197   0.0
SFF587 (SFF587Q) /CSM/SF/SFF5-D/SFF587Q.Seq.d/                       1197   0.0
AFB134 (AFB134Q) /CSM/AF/AFB1-B/AFB134Q.Seq.d/                       1183   0.0
VFN791 (VFN791Q) /CSM/VF/VFN7-D/VFN791Q.Seq.d/                       1181   0.0
SFJ838 (SFJ838Q) /CSM/SF/SFJ8-B/SFJ838Q.Seq.d/                       1179   0.0
VFO810 (VFO810Q) /CSM/VF/VFO8-A/VFO810Q.Seq.d/                       1148   0.0
VFD140 (VFD140Q) /CSM/VF/VFD1-B/VFD140Q.Seq.d/                       1148   0.0
VFA873 (VFA873Q) /CSM/VF/VFA8-D/VFA873Q.Seq.d/                       1148   0.0
SFD415 (SFD415Q) /CSM/SF/SFD4-A/SFD415Q.Seq.d/                       1148   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BM167590|BM167590.1 EST570113 PyBS Plasmodium yoelii yoelii cDNA clone PYCON24 5' end,mRNA sequence.   155   2e-33  1
BM398238|BM398238.1 5009-0-42-F11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    54   9e-28  6
AQ854898|AQ854898.1 CpG2108B CpIOWAgDNA1 Cryptosporidium parvum genomic similar to developmentally regulated GTP-binding protein, genomic survey sequence.   125   2e-24  1
CK250070|CK250070.1 EST733707 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCBD21 5' end, mRNA sequence.    44   4e-24  6
BM401388|BM401388.1 5009-0-9-H05.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    52   7e-24  5
CK272276|CK272276.1 EST718354 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POAD422 5' end, mRNA sequence.    44   8e-21  6
BQ515194|BQ515194.1 EST622609 Generation of a set of potato cDNA clones for microarray analyses mixed potato tissues Solanum tuberosum cDNA clone STMIQ66 3' end, mRNA sequence.    44   8e-15  4
BX561726|BX561726.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse57d12_q1c.    62   1e-14  4
BM170653|BM170653.1 EST573176 PyBS Plasmodium yoelii yoelii cDNA clone PYCQJ35 5' end, mRNA sequence.    90   9e-14  1
AY035034|AY035034.1 Arabidopsis thaliana putative GTP-binding protein (AT4g39520) mRNA, complete cds.    74   1e-13  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK066361_1(AK066361|pid:none) Oryza sativa Japonica Group cDNA c...   281   e-141
EU964619_1(EU964619|pid:none) Zea mays clone 280387 developmenta...   278   e-139
AC146748_16(AC146748|pid:none) Medicago truncatula clone mth2-65...   278   e-138
EU236700_1(EU236700|pid:none) Pisum sativum developmentally regu...   278   e-138
BC053264_1(BC053264|pid:none) Danio rerio developmentally regula...   270   e-134
BT048439_1(BT048439|pid:none) Salmo salar clone ssal-rgb2-564-08...   263   e-133
BT056385_1(BT056385|pid:none) Salmo salar clone ssal-rgb2-554-07...   263   e-132
BC020803_1(BC020803|pid:none) Homo sapiens developmentally regul...   267   e-131
DQ215861_1(DQ215861|pid:none) Taeniopygia guttata clone 0058P004...   266   e-131
AK080814_1(AK080814|pid:none) Mus musculus adult male corpora qu...   267   e-131

 psg: 0.45  gvh: 0.49  alm: 0.32  top: 0.37  tms: 0.07  mit: 0.24  mip: 0.02
 nuc: 0.03  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.11  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 40.0 %: mitochondrial
	 24.0 %: nuclear
	 16.0 %: Golgi
	  8.0 %: cytoplasmic
	  8.0 %: endoplasmic reticulum
	  4.0 %: vacuolar

>> prediction for VFI139 is mit

>VFI139F.Seq
AATTATATATATAGAACATCCTTTCTAAATCGAAATAACTTTAATTAATTAGGTTTTAGT
TTATACAATATATAGGGAGAAAATATATAATACAGAATGAGTCTTGTACAAAAAATCAAA
GAGGTCGAGGATGAGATGGCTCGTACCCAAAAAAATAAAGCTACAAGCTTTCATTTAGGT
GTATTGAAAGCAAAATTAGCAAAATATAAAAGAGAATTATTGTTAGGACCAAGTAAAGGT
GCAGCAGCAGGTGCAGGTGAAGGTTTCGATGTATCAAAAGCAGGTGATGCTCGTGTTGGT
TTAATTGGTTTCCCATCAGTTGGTAAATCAACTTTATTAACTAAATTAACTGGTACCTCA
TCTGAAGTTGCCTCATACGAATTCACAACTTTAACATGTATTCCTGGTGTAATTAATTAT
AAAGGTGCTAAAATTCAATTATTAGATTTACCAGGTATTATTGAAGGTGCAAAAGATGGT
AAAGGTAGAGGTAGACAAGTTATTGCAGTTGGTAGAACATGTAATCTTATTTTAATTGTA
TTGGATGCAATGAAACCATTGGTACATAAAAAGATTATAGAGAGAGAGTTGGATGGTTTT
NNNNNNNNNN

>VFI139Z.Seq
NNNNNNNNNNCCACCACCAATCACATTCAAAAAGAAGGAGAAGGGTGGTATTAACTTTTC
ACATACACCAAATGTCAATCCAACCCAATTGGATAGTGAAACTGTGAAAGCAATCTGTGC
AGAATATAAAATTCACAATGCAGATGTGATTTTACGTGGAAATTGTACAGTGGATGAATT
CATCGATGTCATTGAAGGCAATCGTATCTACGTTCCATGTATTTACGTTTTAAATAAGAT
CGATGCTATCTCTATCGAAGAGTTGGACCTTTTAGATAAAATTCCTCATTATGTTCCAAT
CTCTTCTCATTTGGAATGGAATTTAGATGCCCTCCTCGATAAAATTTGGGAATACTTAAA
ATTGATTAGAGTTTACACTAAACCAAAAGGTCTCATTCCAGATTACAATGAACCAGTCGT
CATTAGAGGTGGTGAAGAAGCTTCAATTGAAACTTTTTGTAATCATATTCATAATTCAAT
TATTAGACAATTTAGATATGCTTTGGTTTGGGGTTCTTCAGCTAAACATAACCCTCAACG
TTGTGGTAAGGATCACGTTTTAGCAGATGAAGATATTGTTCAAATTGTTAAAAAATAAGA
TCACATTTTAATGGNAAAGATAA

>VFI139P.Seq
AATTATATATATAGAACATCCTTTCTAAATCGAAATAACTTTAATTAATTAGGTTTTAGT
TTATACAATATATAGGGAGAAAATATATAATACAGAATGAGTCTTGTACAAAAAATCAAA
GAGGTCGAGGATGAGATGGCTCGTACCCAAAAAAATAAAGCTACAAGCTTTCATTTAGGT
GTATTGAAAGCAAAATTAGCAAAATATAAAAGAGAATTATTGTTAGGACCAAGTAAAGGT
GCAGCAGCAGGTGCAGGTGAAGGTTTCGATGTATCAAAAGCAGGTGATGCTCGTGTTGGT
TTAATTGGTTTCCCATCAGTTGGTAAATCAACTTTATTAACTAAATTAACTGGTACCTCA
TCTGAAGTTGCCTCATACGAATTCACAACTTTAACATGTATTCCTGGTGTAATTAATTAT
AAAGGTGCTAAAATTCAATTATTAGATTTACCAGGTATTATTGAAGGTGCAAAAGATGGT
AAAGGTAGAGGTAGACAAGTTATTGCAGTTGGTAGAACATGTAATCTTATTTTAATTGTA
TTGGATGCAATGAAACCATTGGTACATAAAAAGATTATAGAGAGAGAGTTGGATGGTTTT
----------CCACCACCAATCACATTCAAAAAGAAGGAGAAGGGTGGTATTAACTTTTC
ACATACACCAAATGTCAATCCAACCCAATTGGATAGTGAAACTGTGAAAGCAATCTGTGC
AGAATATAAAATTCACAATGCAGATGTGATTTTACGTGGAAATTGTACAGTGGATGAATT
CATCGATGTCATTGAAGGCAATCGTATCTACGTTCCATGTATTTACGTTTTAAATAAGAT
CGATGCTATCTCTATCGAAGAGTTGGACCTTTTAGATAAAATTCCTCATTATGTTCCAAT
CTCTTCTCATTTGGAATGGAATTTAGATGCCCTCCTCGATAAAATTTGGGAATACTTAAA
ATTGATTAGAGTTTACACTAAACCAAAAGGTCTCATTCCAGATTACAATGAACCAGTCGT
CATTAGAGGTGGTGAAGAAGCTTCAATTGAAACTTTTTGTAATCATATTCATAATTCAAT
TATTAGACAATTTAGATATGCTTTGGTTTGGGGTTCTTCAGCTAAACATAACCCTCAACG
TTGTGGTAAGGATCACGTTTTAGCAGATGAAGATATTGTTCAAATTGTTAAAAAATAAGA
TCACATTTTAATGGNAAAGATAA