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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFI106
Library VF
(Link to library)
Clone ID VFI106
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16451-1
Original site URL
Representative seq. ID VFI106P
(Link to Original site)
Representative DNA sequence 
>VFI106 (VFI106Q) /CSM/VF/VFI1-A/VFI106Q.Seq.d/
AACTCGACCTAACAAATAAAAAAAGCAAAATGCAAAATAAACCATCATTCATTTTAAGAA
ACCCATCAGCTAATAAAGGTACTGGTTTTAATAATGAAGAAAGAGAAAAATTAGGATTAA
AAGGTTTATTACCACCAAAGGTTGAATCATTACAAGAACAATCAGATAGAGCATTATCAC
AATTTACATCATTTAATACAAACTTGGAAAGATATATTTTCTTAAATTGTTTACGTGATC
GTAATGAAACTTTATTCTATTATTTATTAAGTAATAATTTAGAATTAATGATGCCAATCA
TTTATACACCAACCGTAGGTGAAGCATGTCAAAAGTTTGGTAATGAATTCCGTTTCGCCC
AAGGTATGTATTTTGCATCCCAAGATAAAGGTAACATTCGTGCCATGATGGACAATTGGC
CAGCCGAAGGTGTCGATATTATCGTTGTTTCCGATGGTTCACGTATTTTGGGTTTAGGTG
ATCTCGGTACCAACGGTATGGGTATCCCAGTTGGTAAGTTACAATTGTACGTCGCCGGTG
CTGGTTTCTGTCCAACCCGTACACTCCCAGTCATTATCGATAGTGGTACAAACACCAAGA
AXXXXXXXXXXTTGAAATCATTATTAGAGGTTGTCCGTGACGTTAAGCCAACCGCTATCA
TTGGTTTATCAGGTATCGGTGGTTCATTCTCTCAAGAAGTAATTGAAGAAATGGCCAAAC
ACGTCGAGAAACCAATCGTTTTCGCTCTCTCAAATCCAACCACCAACGCTGAATGTACCG
CAGAACAAGCTTACCAATGGACCGATGGTCGTTGTATCTTTGCCTCTGGTTCACCATTCA
AACCAGTCGAATACAAAGGTAAAACCTTTGTTCCAGGTCAAGGTAACAATATGTACATCT
TCCCAGGTCTTGGTTTAGCCGCTAGCGTTTGTGAAGCTAAACATGTCACTGATGCTATGA
TCATTACTGCCGCTAAAACTCTTGCCTCCTTTGTAGAAGACTCTGAAGTTCTCACTGGTA
AAATCTATCCAGGTCTCCAACATATTCGTGAAATCTCAACAAGAATCGCCGTTAAAGTCA
TTGAAAAAGCTTATGAAGAAGGTATGGCTCAATTACCACGTCCAGATAATATTGAAGCTT
TAGTCAAATCTCGTCAATATGTTCCATCTTATGATAAATCAAAAAATTGACTATCTTGCC
CAATTTCACCAAATACAAATAGATTAAGTNCAAAGTTA
sequence update 2001.11.22
Translated Amino Acid sequence 
LDLTNKKSKMQNKPSFILRNPSANKGTGFNNEEREKLGLKGLLPPKVESLQEQSDRALSQ
FTSFNTNLERYIFLNCLRDRNETLFYYLLSNNLELMMPIIYTPTVGEACQKFGNEFRFAQ
GMYFASQDKGNIRAMMDNWPAEGVDIIVVSDGSRILGLGDLGTNGMGIPVGKLQLYVAGA
GFCPTRTLPVIIDSGTNTK---

---LKSLLEVVRDVKPTAIIGLSGIGGSFSQEVIEEMAKHVEKPIVFALSNPTTNAECTA
EQAYQWTDGRCIFASGSPFKPVEYKGKTFVPGQGNNMYIFPGLGLAASVCEAKHVTDAMI
ITAAKTLASFVEDSEVLTGKIYPGLQHIREISTRIAVKVIEKAYEEGMAQLPRPDNIEAL
VKSRQYVPSYDKSKN*lscpispntnrlsxkl
Translated Amino Acid sequence (All Frames) 
Frame A:
nst*qikkakckinhhsf*ethqlikvlvlimkkekn*d*kvyyhqrlnhyknnqiehyh
nlhhliqtwkdifs*ivyvivmklysiiy*vii*n**cqsfihqp*vkhvkslvmnsvsp
kvcilhpkikvtfvp*wtigqpkvsilslfpmvhvfwv*visvptvwvsqlvsynctspv
lvsvqpvhsqslsivvqtpr---

---LKSLLEVVRDVKPTAIIGLSGIGGSFSQEVIEEMAKHVEKPIVFALSNPTTNAECTA
EQAYQWTDGRCIFASGSPFKPVEYKGKTFVPGQGNNMYIFPGLGLAASVCEAKHVTDAMI
ITAAKTLASFVEDSEVLTGKIYPGLQHIREISTRIAVKVIEKAYEEGMAQLPRPDNIEAL
VKSRQYVPSYDKSKN*lscpispntnrlsxkl

Frame B:
trpnk*kkqnak*tiihfkkpis**rywf***rkrkirikrfittkg*iitrtir*siit
iyii*yklgkiyflklft*s**nfillfik**frindanhlytnrr*smskvw**ipfrp
ryvfcipr*r*hschdgqlasrrcryyrcfrwftyfgfr*sryqrygypsw*vtivrrrc
wflsnpytpshyr*wykhqe---

---*nhy*rlsvtlsqplslvyqvsvvhslkk*lkkwpntsrnqsfslsqiqpptlnvpq
nkltngpmvvvslplvhhsnqsntkvkplfqvkvtictssqvlv*plafvklnmslml*s
llplkllppl*ktlkfslvksiqvsnifvksqqesplkslkklmkkvwlnyhvqiilkl*
snlvnmfhlminqkidylaqfhqiqid*vqs

Frame C:
LDLTNKKSKMQNKPSFILRNPSANKGTGFNNEEREKLGLKGLLPPKVESLQEQSDRALSQ
FTSFNTNLERYIFLNCLRDRNETLFYYLLSNNLELMMPIIYTPTVGEACQKFGNEFRFAQ
GMYFASQDKGNIRAMMDNWPAEGVDIIVVSDGSRILGLGDLGTNGMGIPVGKLQLYVAGA
GFCPTRTLPVIIDSGTNTK---

---eiiirgcp*r*anryhwfiryrwfilsrsn*rngqtrretnrfrslksnhqr*myrr
tslpmdrwslylclwftiqtsriqr*nlcsrsr*qyvhlprswfsr*rl*s*tch*cydh
ycr*nscllcrrl*sshw*nlsrsptys*nlnknrr*sh*ksl*rrygsittsr*y*sfs
qissicsil**ikkltilpnftkyk*ikxkv
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFI106 (VFI106Q) /CSM/VF/VFI1-A/VFI106Q.Seq.d/                       2347   0.0
SLB437 (SLB437Q) /CSM/SL/SLB4-B/SLB437Q.Seq.d/                       1237   0.0
CHB626 (CHB626Q) /CSM/CH/CHB6-B/CHB626Q.Seq.d/                       1237   0.0
CFJ334 (CFJ334Q) /CSM/CF/CFJ3-B/CFJ334Q.Seq.d/                       1221   0.0
SFD205 (SFD205Q) /CSM/SF/SFD2-A/SFD205Q.Seq.d/                       1219   0.0
VFK276 (VFK276Q) /CSM/VF/VFK2-D/VFK276Q.Seq.d/                       1211   0.0
CHA431 (CHA431Q) /CSM/CH/CHA4-B/CHA431Q.Seq.d/                       1168   0.0
CFG469 (CFG469Q) /CSM/CF/CFG4-C/CFG469Q.Seq.d/                       1150   0.0
CFH782 (CFH782Q) /CSM/CF/CFH7-D/CFH782Q.Seq.d/                       1142   0.0
CHA161 (CHA161Q) /CSM/CH/CHA1-C/CHA161Q.Seq.d/                       1126   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY387644|AY387644.1 Dictyostelium discoideum malic enzyme gene, complete cds.  1237   0.0    3
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.  1237   0.0    6
AR375993|AR375993.1 Sequence 999 from patent US 6605709.           46   7e-05  4
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    36   0.002  17
Y09212|Y09212.1 B.cereus partial gltT, aspA and partial maoX genes.    52   0.019  1
AE017029|AE017029.1 Bacillus anthracis str. Ames section 6 of 18 of the complete genome.    52   0.019  1
AY194854|AY194854.1 Meloidogyne mayaguensis 5S-18S ribosomal RNA intergenic spacer, partial sequence.    40   0.047  2
BP506190|BP506190.1 Hydra magnipapillata cDNA, clone:hm_04308.     42   0.070  2
BQ120661|BQ120661.2 EST606237 mixed potato tissues Solanum tuberosum cDNA clone STMES17 5' end, mRNA sequence.    44   0.082  2
CK272664|CK272664.1 EST718742 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POAD658 3' end, mRNA sequence.    44   0.086  2
dna update 2004. 1.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP001322_605(CP001322|pid:none) Desulfatibacillum alkenivorans A...   193   9e-48
AY149910_1(AY149910|pid:none) Mastigamoeba balamuthi malic enzym...   190   8e-47
AY594688_1(AY594688|pid:none) Hydrilla verticillata NADP-depende...   187   5e-46
(P34105) RecName: Full=NADP-dependent malic enzyme;          Sho...   187   5e-46
DQ901690_1(DQ901690|pid:none) Tigriopus californicus haplotype S...   186   1e-45
CP001032_3280(CP001032|pid:none) Opitutus terrae PB90-1, complet...   186   1e-45
DQ901654_1(DQ901654|pid:none) Tigriopus californicus haplotype A...   186   1e-45
DQ901663_1(DQ901663|pid:none) Tigriopus californicus haplotype A...   186   1e-45
DQ901691_1(DQ901691|pid:none) Tigriopus californicus haplotype S...   186   1e-45
DQ901669_1(DQ901669|pid:none) Tigriopus californicus haplotype S...   186   1e-45
protein update 2009. 6.21
PSORT 

 psg: 0.68  gvh: 0.55  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 32.0 %: mitochondrial
	 24.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for VFI106 is cyt
5' end seq. ID VFI106F
5' end seq. 
>VFI106F.Seq
AACTCGACCTAACAAATAAAAAAAGCAAAATGCAAAATAAACCATCATTCATTTTAAGAA
ACCCATCAGCTAATAAAGGTACTGGTTTTAATAATGAAGAAAGAGAAAAATTAGGATTAA
AAGGTTTATTACCACCAAAGGTTGAATCATTACAAGAACAATCAGATAGAGCATTATCAC
AATTTACATCATTTAATACAAACTTGGAAAGATATATTTTCTTAAATTGTTTACGTGATC
GTAATGAAACTTTATTCTATTATTTATTAAGTAATAATTTAGAATTAATGATGCCAATCA
TTTATACACCAACCGTAGGTGAAGCATGTCAAAAGTTTGGTAATGAATTCCGTTTCGCCC
AAGGTATGTATTTTGCATCCCAAGATAAAGGTAACATTCGTGCCATGATGGACAATTGGC
CAGCCGAAGGTGTCGATATTATCGTTGTTTCCGATGGTTCACGTATTTTGGGTTTAGGTG
ATCTCGGTACCAACGGTATGGGTATCCCAGTTGGTAAGTTACAATTGTACGTCGCCGGTG
CTGGTTTCTGTCCAACCCGTACACTCCCAGTCATTATCGATAGTGGTACAAACACCAAGA
ANNNNNNNNNN
Length of 5' end seq. 611
3' end seq. ID VFI106Z
3' end seq. 
>VFI106Z.Seq
NNNNNNNNNNTTGAAATCATTATTAGAGGTTGTCCGTGACGTTAAGCCAACCGCTATCAT
TGGTTTATCAGGTATCGGTGGTTCATTCTCTCAAGAAGTAATTGAAGAAATGGCCAAACA
CGTCGAGAAACCAATCGTTTTCGCTCTCTCAAATCCAACCACCAACGCTGAATGTACCGC
AGAACAAGCTTACCAATGGACCGATGGTCGTTGTATCTTTGCCTCTGGTTCACCATTCAA
ACCAGTCGAATACAAAGGTAAAACCTTTGTTCCAGGTCAAGGTAACAATATGTACATCTT
CCCAGGTCTTGGTTTAGCCGCTAGCGTTTGTGAAGCTAAACATGTCACTGATGCTATGAT
CATTACTGCCGCTAAAACTCTTGCCTCCTTTGTAGAAGACTCTGAAGTTCTCACTGGTAA
AATCTATCCAGGTCTCCAACATATTCGTGAAATCTCAACAAGAATCGCCGTTAAAGTCAT
TGAAAAAGCTTATGAAGAAGGTATGGCTCAATTACCACGTCCAGATAATATTGAAGCTTT
AGTCAAATCTCGTCAATATGTTCCATCTTATGATAAATCAAAAAATTGACTATCTTGCCC
AATTTCACCAAATACAAATAGATTAAGTNCAAAGTTA
Length of 3' end seq. 637
Connected seq. ID VFI106P
Connected seq. 
>VFI106P.Seq
AACTCGACCTAACAAATAAAAAAAGCAAAATGCAAAATAAACCATCATTCATTTTAAGAA
ACCCATCAGCTAATAAAGGTACTGGTTTTAATAATGAAGAAAGAGAAAAATTAGGATTAA
AAGGTTTATTACCACCAAAGGTTGAATCATTACAAGAACAATCAGATAGAGCATTATCAC
AATTTACATCATTTAATACAAACTTGGAAAGATATATTTTCTTAAATTGTTTACGTGATC
GTAATGAAACTTTATTCTATTATTTATTAAGTAATAATTTAGAATTAATGATGCCAATCA
TTTATACACCAACCGTAGGTGAAGCATGTCAAAAGTTTGGTAATGAATTCCGTTTCGCCC
AAGGTATGTATTTTGCATCCCAAGATAAAGGTAACATTCGTGCCATGATGGACAATTGGC
CAGCCGAAGGTGTCGATATTATCGTTGTTTCCGATGGTTCACGTATTTTGGGTTTAGGTG
ATCTCGGTACCAACGGTATGGGTATCCCAGTTGGTAAGTTACAATTGTACGTCGCCGGTG
CTGGTTTCTGTCCAACCCGTACACTCCCAGTCATTATCGATAGTGGTACAAACACCAAGA
A----------TTGAAATCATTATTAGAGGTTGTCCGTGACGTTAAGCCAACCGCTATCA
TTGGTTTATCAGGTATCGGTGGTTCATTCTCTCAAGAAGTAATTGAAGAAATGGCCAAAC
ACGTCGAGAAACCAATCGTTTTCGCTCTCTCAAATCCAACCACCAACGCTGAATGTACCG
CAGAACAAGCTTACCAATGGACCGATGGTCGTTGTATCTTTGCCTCTGGTTCACCATTCA
AACCAGTCGAATACAAAGGTAAAACCTTTGTTCCAGGTCAAGGTAACAATATGTACATCT
TCCCAGGTCTTGGTTTAGCCGCTAGCGTTTGTGAAGCTAAACATGTCACTGATGCTATGA
TCATTACTGCCGCTAAAACTCTTGCCTCCTTTGTAGAAGACTCTGAAGTTCTCACTGGTA
AAATCTATCCAGGTCTCCAACATATTCGTGAAATCTCAACAAGAATCGCCGTTAAAGTCA
TTGAAAAAGCTTATGAAGAAGGTATGGCTCAATTACCACGTCCAGATAATATTGAAGCTT
TAGTCAAATCTCGTCAATATGTTCCATCTTATGATAAATCAAAAAATTGACTATCTTGCC
CAATTTCACCAAATACAAATAGATTAAGTNCAAAGTTA
Length of connected seq. 1228
Full length Seq ID -
Full length Seq. -
Length of full length seq. -