NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFA213
Library AF
(Link to library)
Clone ID AFA213
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13825-1
Original site URL
Representative seq. ID AFA213P
(Link to Original site)
Representative DNA sequence 
>AFA213 (AFA213Q) /CSM/AF/AFA2-A/AFA213Q.Seq.d/
ACTGTTGGCCTACTGGGTAAATATTAATATAATTAAAAATGTTAAGAATTAACAGGAATT
TCTAACTTACGTACTTTTGGTCAACGTTTCTTCTCAACTGAACAACAAGATGTTGTTGTT
ATTGGTGGTGGACCAGGTGGTTATGTTGCAGGTATTAAAGCAGGTCAATTAGGTATGAAA
GTTACAGTTGTAGAGAAAAGAGGTAAATTAGGAGGAACATGTTTAAATGTTGGTTGTATT
CCATCAAAAGCATTATTGAATGCATCACATCTTTATGAAGAAGCCACCACTAAAATGTCC
AAATATGGNGTCAAATGTTCAGGTGTTGAATTAGATTTGGGAGCAATGATGCAATACAAA
GATAAXXXXXXXXXXTCNCAATGGTGGTGGTCATGTTAACNATGGNGCCATCCCATCCAT
TATNTACACTCACCCAAAAGTTGCTTGGGTTGGTAAGACTNAANAANAATTACAAAAAGA
AGGTATCCAATTCAATNTTGGTANATTCCCATTNGTTGCAAACTCTANAGCCAAAACAAA
CGATGATGTCGAAGGTTTCGTTAAATTCCTCNCCGCTAAAGATTCTGATANAGTCCTTGG
NGCTCATATTATGGGTACCAATGCTGGTGAATTAATTGGTGAATGNGTTTTAGCTATGGA
ATATGGTGCCNCATGTGAAGATATTGCAAGAACCTGTCATGGTCATCCAACTTTATACCG
AAGCTGTTAAAGAAGCTGCTATGGATGCCTACGATAAGCCNAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
tvgllgky*yn*kc*ELTGISNLRTFGQRFFSTEQQDVVVIGGGPGGYVAGIKAGQLGMK
VTVVEKRGKLGGTCLNVGCIPSKALLNASHLYEEATTKMSKYGVKCSGVELDLGAMMQYK
D---

---XNGGGHVNXGAIPSIXYTHPKVAWVGKTXXXLQKEGIQFNXGXFPXVANSXAKTNDD
VEGFVKFLXAKDSDXVLGAHIMGTNAGELIGEXVLAMEYGAXCEDIARTCHGHPTLYRSC
*rscygclr*ax
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllgky*yn*kc*ELTGISNLRTFGQRFFSTEQQDVVVIGGGPGGYVAGIKAGQLGMK
VTVVEKRGKLGGTCLNVGCIPSKALLNASHLYEEATTKMSKYGVKCSGVELDLGAMMQYK
D---

---sqwwwsc*xwxhpihyxhspksclgw*dxxxitkrrypiqxwxipixcklxsqnkr*
crrfr*ipxr*rf*xspwxsyygyqcw*inw*mxfsygiwcxm*rycknlswssnfipkl
lkkllwmptisx

Frame B:
llaywvniniiknvkn*qefltyvllvnvssqlnnkmllllvvdqvvmlqvlkqvn*v*k
lql*rkevn*eehv*mlvvfhqkhy*mhhifmkkpplkcpnmxsnvqvln*iweq*cntk
i---

---XNGGGHVNXGAIPSIXYTHPKVAWVGKTXXXLQKEGIQFNXGXFPXVANSXAKTNDD
VEGFVKFLXAKDSDXVLGAHIMGTNAGELIGEXVLAMEYGAXCEDIARTCHGHPTLYRSC
*rscygclr*ax

Frame C:
cwptg*ili*lkmlrinrnf*ltyfwstflln*ttrcccywwwtrwlccry*srsiryes
yscrekr*irrnmfkcwlysiksiiecitsl*rshh*nvqiwxqmfrc*irfgsndaiqr
*---

---xmvvvmltmxpshplxtltqkllglvrlxxnykkkvsnsxlvxshxlqtlxpkqtmm
skvslnssplkilixslxlilwvpmlvn*lvnxf*lwnmvphvkilqepvmviqlyteav
keaamdaydkp
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFA213 (AFA213Q) /CSM/AF/AFA2-A/AFA213Q.Seq.d/                       1346   0.0
SFH666 (SFH666Q) /CSM/SF/SFH6-C/SFH666Q.Seq.d/                        702   0.0
VFC152 (VFC152Q) /CSM/VF/VFC1-C/VFC152Q.Seq.d/                        698   0.0
VFH735 (VFH735Q) /CSM/VF/VFH7-B/VFH735Q.Seq.d/                        690   0.0
AFA139 (AFA139Q) /CSM/AF/AFA1-B/AFA139Q.Seq.d/                        682   0.0
VFL340 (VFL340Q) /CSM/VF/VFL3-B/VFL340Q.Seq.d/                        670   0.0
VFI130 (VFI130Q) /CSM/VF/VFI1-B/VFI130Q.Seq.d/                        670   0.0
VFE880 (VFE880Q) /CSM/VF/VFE8-D/VFE880Q.Seq.d/                        670   0.0
VFE339 (VFE339Q) /CSM/VF/VFE3-B/VFE339Q.Seq.d/                        670   0.0
VFD715 (VFD715Q) /CSM/VF/VFD7-A/VFD715Q.Seq.d/                        670   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AX489387|AX489387.1 Sequence 6687 from Patent WO02053728.         52  8e-18  3
AR180935|AR180935.1 Sequence 7 from patent US 6333164.            52  2e-15  3
BG227132|BG227132.1 kp97d11.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:LLC1.3 CE16262 DIHYDROLIPOAMIDE DEHYDROGENASE ;, mRNA sequence.    62  2e-11  2
BQ622732|BQ622732.1 CC_Contig28 Conidiobolus cornatus ARSEF 512 Conidiobolus coronatus cDNA, mRNA sequence.    66  4e-09  2
AP001118|AP001118.1 Buchnera sp. APS genomic DNA, complete sequence, segment 1/2.    60  1e-08  4
BM493927|BM493927.1 NXLV_071_F02_F NXLV (Nsf Xylem Late wood Vertical) Pinus taeda cDNA clone NXLV_071_F02 5', mRNA sequence.    50  5e-08  2
BQ634402|BQ634402.1 NXRV068_E06_F NXRV (Nsf Xylem Root wood Vertical) Pinus taeda cDNA clone NXRV068_E06 5', mRNA sequence.    50  6e-08  2
BI416760|BI416760.1 hasp002xc23f Heterobasidion annosum - Scots pine infection stage subtraction cDNA library (hasp) Mixed Heterobasidion annosum/Pinus sylvestris library cDNA clone hasp002xc23f, mRNA sequence.    50  7e-08  2
AL423544|AL423544.1 T3 end of clone AZ0AA009B01 of library AZ0AA from strain CBS 712 of Kluyveromyces marxianus.    64  1e-07  2
BD061520|BD061520.1 Genome DNA of symbiotic bacteria of aphid.    60  6e-07  6
dna update 2003. 7.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CR382130_836(CR382130|pid:none) Yarrowia lipolytica strain CLIB1...   155   4e-37
AY710429_12(AY710429|pid:none) Cryptococcus gattii strain E566 M...   150   1e-36
AF542528_19(AF542528|pid:none) Cryptococcus neoformans var. grub...   149   4e-36
AY710430_14(AY710430|pid:none) Cryptococcus gattii strain WM276 ...   149   4e-36
AE017344_576(AE017344|pid:none) Cryptococcus neoformans var. neo...   148   7e-35
CP000501_129(CP000501|pid:none) Pichia stipitis CBS 6054 chromos...   146   1e-34
AP007171_417(AP007171|pid:none) Aspergillus oryzae RIB40 genomic...   148   2e-34
CR382124_462(CR382124|pid:none) Kluyveromyces lactis strain NRRL...   148   2e-34
DQ674546_1(DQ674546|pid:none) Coccidioides posadasii dihydrolipo...   147   4e-34
AY391421_1(AY391421|pid:none) Danio rerio dihydrolipoamide dehyd...   146   6e-34
protein update 2009. 3.25
PSORT 

 psg: 0.73  gvh: 0.36  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for AFA213 is cyt
5' end seq. ID AFA213F
5' end seq. 
>AFA213F.Seq
ACTGTTGGCCTACTGGGTAAATATTAATATAATTAAAAATGTTAAGAATTAACAGGAATT
TCTAACTTACGTACTTTTGGTCAACGTTTCTTCTCAACTGAACAACAAGATGTTGTTGTT
ATTGGTGGTGGACCAGGTGGTTATGTTGCAGGTATTAAAGCAGGTCAATTAGGTATGAAA
GTTACAGTTGTAGAGAAAAGAGGTAAATTAGGAGGAACATGTTTAAATGTTGGTTGTATT
CCATCAAAAGCATTATTGAATGCATCACATCTTTATGAAGAAGCCACCACTAAAATGTCC
AAATATGGNGTCAAATGTTCAGGTGTTGAATTAGATTTGGGAGCAATGATGCAATACAAA
GATAA----------
Length of 5' end seq. 365
3' end seq. ID AFA213Z
3' end seq. 
>AFA213Z.Seq
----------TCNCAATGGTGGTGGTCATGTTAACNATGGNGCCATCCCATCCATTATNT
ACACTCACCCAAAAGTTGCTTGGGTTGGTAAGACTNAANAANAATTACAAAAAGAAGGTA
TCCAATTCAATNTTGGTANATTCCCATTNGTTGCAAACTCTANAGCCAAAACAAACGATG
ATGTCGAAGGTTTCGTTAAATTCCTCNCCGCTAAAGATTCTGATANAGTCCTTGGNGCTC
ATATTATGGGTACCAATGCTGGTGAATTAATTGGTGAATGNGTTTTAGCTATGGAATATG
GTGCCNCATGTGAAGATATTGCAAGAACCTGTCATGGTCATCCAACTTTATACCGAAGCT
GTTAAAGAAGCTGCTATGGATGCCTACGATAAGCCNAT
Length of 3' end seq. 388
Connected seq. ID AFA213P
Connected seq. 
>AFA213P.Seq
ACTGTTGGCCTACTGGGTAAATATTAATATAATTAAAAATGTTAAGAATTAACAGGAATT
TCTAACTTACGTACTTTTGGTCAACGTTTCTTCTCAACTGAACAACAAGATGTTGTTGTT
ATTGGTGGTGGACCAGGTGGTTATGTTGCAGGTATTAAAGCAGGTCAATTAGGTATGAAA
GTTACAGTTGTAGAGAAAAGAGGTAAATTAGGAGGAACATGTTTAAATGTTGGTTGTATT
CCATCAAAAGCATTATTGAATGCATCACATCTTTATGAAGAAGCCACCACTAAAATGTCC
AAATATGGNGTCAAATGTTCAGGTGTTGAATTAGATTTGGGAGCAATGATGCAATACAAA
GATAA----------TCNCAATGGTGGTGGTCATGTTAACNATGGNGCCATCCCATCCAT
TATNTACACTCACCCAAAAGTTGCTTGGGTTGGTAAGACTNAANAANAATTACAAAAAGA
AGGTATCCAATTCAATNTTGGTANATTCCCATTNGTTGCAAACTCTANAGCCAAAACAAA
CGATGATGTCGAAGGTTTCGTTAAATTCCTCNCCGCTAAAGATTCTGATANAGTCCTTGG
NGCTCATATTATGGGTACCAATGCTGGTGAATTAATTGGTGAATGNGTTTTAGCTATGGA
ATATGGTGCCNCATGTGAAGATATTGCAAGAACCTGTCATGGTCATCCAACTTTATACCG
AAGCTGTTAAAGAAGCTGCTATGGATGCCTACGATAAGCCNAT
Length of connected seq. 753
Full length Seq ID -
Full length Seq. -
Length of full length seq. -