NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFH703
Library VF
(Link to library)
Clone ID VFH703
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VFH703P
(Link to Original site)
Representative DNA sequence 
>VFH703 (VFH703Q) /CSM/VF/VFH7-A/VFH703Q.Seq.d/
ACTGTTGGCCTACTGGGTTATCATCCAATGTCAGTATAAACCAACATCAATTTTAAAGAA
TTATGAAATTGGATATATTTCATCATATGAACATCCAAAATTTGTTCAAAAATCAGTATT
TCTACAAGTACATTAAAAGAGAGAGTTAGAAAACATTTTAAAGCAACCGATCAAGATCCA
CAAATGGCAGTTAGTATTTTCTCACGTTTAGCATTGGTTTATCTTTTAGTGTTTGTAACT
TACTATTTAGCACATTATACCAGTAACAATTTTTATTTAAATTGTTTCTTGGCGATCGTT
TACGCACTTTGCAACTCATTATTCTCAATGCATATGATGCATGATTCATGTCATGCTGCC
ATTTCACATTATCCAGGCGTATGGAAATGGATGGGCGCATCATTTGATTTCGTCACTGGT
GCTTCATTCCTTTCATGGTGTCATCAACATGTAATTGGTCATCATATTTACACCAATGTA
AGAAATGCCGATCCAGATCTTGGTCAAGGTGAGGTCCGATTTCCGTATTGXXXXXXXXXX
TATTTACACACTCAAATATNGTACTCAAGATTGGGAAGCTTTTGTAAAGNATGGTAAAAA
TGGTGCAATTCGTGTTAGTGTCGCCACAAATTTCGATAAGGCCGCTTACGTCATTGGTAA
ATTGTCTTTTGTTTTCTTCCGTTTCATCCTTCCACTCCGTTATCATAGCTTTACAGATTT
AATTTGTTATTTCCTCATTGCTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCA
AGTTAGTCATGTCGCTGAAGATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACC
ATCTCAAATCAATGAAGATTGGGCAATCCTTCAACTTANAACTACTCAAGATTATGGTCA
TGGTTCACTCCTTTGTACCTTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTT
ATTCCCATCAATTGCTCAAGATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTG
TAAAGAACATAACATTACTTACCACATTAAACCAAACTTCACTGAAGCTATTATGTCACA
CATTAATTACCTTTACAAAATGGGTAATGATCCAGATTATGTTAAAAAACCATTAGCCTC
AAAAGA
sequence update 2001.11.22
Translated Amino Acid sequence 
tvgllgyhpmsv*tninfkel*nwiyfii*TSKICSKISISTSTLKERVRKHFKATDQDP
QMAVSIFSRLALVYLLVFVTYYLAHYTSNNFYLNCFLAIVYALCNSLFSMHMMHDSCHAA
ISHYPGVWKWMGASFDFVTGASFLSWCHQHVIGHHIYTNVRNADPDLGQGEVRFPY---

---IYTLKYXTQDWEAFVKXGKNGAIRVSVATNFDKAAYVIGKLSFVFFRFILPLRYHSF
TDLICYFLIAEFVFGWYLTINFQVSHVAEDLKFFATPERPDEPSQINEDWAILQLXTTQD
YGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLVPIVKEVCKEHNITYHIKPNFTEAI
MSHINYLYKMGNDPDYVKKPLASK
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllgyhpmsv*tninfkel*nwiyfii*TSKICSKISISTSTLKERVRKHFKATDQDP
QMAVSIFSRLALVYLLVFVTYYLAHYTSNNFYLNCFLAIVYALCNSLFSMHMMHDSCHAA
ISHYPGVWKWMGASFDFVTGASFLSWCHQHVIGHHIYTNVRNADPDLGQGEVRFPY---

---ylhtqixysrlgsfckxw*kwcnsc*crhkfr*grlrhw*ivfcflpfhpstpls*l
yrfnllfphc*irlwlvshn*fps*scr*rsqilcyp*ktr*tisnq*rlgnpstxnysr
lwswftplylf*wffkssscssfipincsrflpttctnckrsl*rt*hylph*tklh*sy
yvth*lplqng**srlc*ktislkr

Frame B:
llaywviiqcqykptsilknyeigyissyehpkfvqksvflqvh*krelenilkqpikih
kwqlvfshv*hwfif*cl*lti*hiipvtifi*ivswrsfthfathysqci*cmihvmlp
fhiiqaygngwahhlisslvlhsfhgvinm*lviiftpm*empiqilvkvrsdfri---

---IYTLKYXTQDWEAFVKXGKNGAIRVSVATNFDKAAYVIGKLSFVFFRFILPLRYHSF
TDLICYFLIAEFVFGWYLTINFQVSHVAEDLKFFATPERPDEPSQINEDWAILQLXTTQD
YGHGSLLCTFFSGSLNHQVVHHLFPSIAQDFYPQLVPIVKEVCKEHNITYHIKPNFTEAI
MSHINYLYKMGNDPDYVKKPLASK

Frame C:
cwptglssnvsinqhqf*rimkldifhhmniqnlfknqyfykyikres*ktf*snrsrst
ngs*yfltfsiglsfsvcnllfstlyq*qflfklflgdrlrtlqliilnayda*fmscch
ftlsrrmemdgrii*frhwcfipfmvsstcnwssylhqckkcrsrswsr*gpisvl---

---fthsnxvlkigkll*xmvkmvqfvlvspqisirpltslvncllfssvssfhsviial
qi*fvissllnsslvgisqlisklvmslkisnsllplkdqmnhlksmkigqsfnlxllki
mvmvhsfvpflvvl*iiklfiiyshqllkisthnlyql*kkfvknitllttlnqtslkll
chtlitftkwvmiqimlknh*pqk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFH703 (VFH703Q) /CSM/VF/VFH7-A/VFH703Q.Seq.d/                       2194   0.0
VFN123 (VFN123Q) /CSM/VF/VFN1-A/VFN123Q.Seq.d/                       1183   0.0
VFM295 (VFM295Q) /CSM/VF/VFM2-D/VFM295Q.Seq.d/                       1183   0.0
VFI562 (VFI562Q) /CSM/VF/VFI5-C/VFI562Q.Seq.d/                       1183   0.0
VFE188 (VFE188Q) /CSM/VF/VFE1-D/VFE188Q.Seq.d/                       1183   0.0
SSB813 (SSB813Q) /CSM/SS/SSB8-A/SSB813Q.Seq.d/                       1183   0.0
SLA259 (SLA259Q) /CSM/SL/SLA2-C/SLA259Q.Seq.d/                       1183   0.0
SLA256 (SLA256Q) /CSM/SL/SLA2-C/SLA256Q.Seq.d/                       1183   0.0
CFC256 (CFC256Q) /CSM/CF/CFC2-C/CFC256Q.Seq.d/                       1183   0.0
VFO207 (VFO207Q) /CSM/VF/VFO2-A/VFO207Q.Seq.d/                       1176   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BD092935|BD092935.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids in plants.  1183   0.0    1
BD082645|BD082645.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids.  1183   0.0    1
BD082630|BD082630.1 Methods and compositions for synthesis of long chain poly-unsaturated fatty acids.  1183   0.0    1
AB029311|AB029311.1 Dictyostelium discoideum Dd des5 gene for fatty acid desaturase, complete cds.   817   0.0    6
AB022097|AB022097.1 Dictyostelium discoideum mRNA for delta 5 fatty acid desaturase, complete cds.    98   1e-90  11
AL589727|AL589727.4 Human DNA sequence from clone RP11-104N13 on chromosome 6.    34   0.022  8
AC009403|AC009403.5 Homo sapiens clone RP5-1015O24, complete sequence.    48   0.19   6
CF595293|CF595293.1 AGENCOURT_15714427 NIH_ZGC_8 Danio rerio cDNA clone IMAGE:7015343 5', mRNA sequence.    48   0.27   1
AC129112|AC129112.4 Rattus norvegicus clone CH230-265O9, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    48   0.27   1
AC137050|AC137050.1 Rattus norvegicus clone CH230-unknown, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    48   0.27   1
dna update 2004. 1.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9Y1W0) RecName: Full=Delta(5) fatty acid desaturase A;        ...   414   0.0
(O96099) RecName: Full=Delta(5) fatty acid desaturase B;        ...   271   e-115
(Q1ZXQ5) RecName: Full=Probable Delta(5) fatty acid desaturase C...   224   e-103
AF419297_1(AF419297|pid:none) Pythium irregulare delta-5 fatty a...   145   2e-53
AB188307_1(AB188307|pid:none) Mortierella alpina gene for delta5...   142   4e-51
(O74212) RecName: Full=Delta(5) fatty acid desaturase;          ...   141   7e-51
CP001574_328(CP001574|pid:none) Micromonas sp. RCC299 chromosome...   125   6e-39
CP000592_278(CP000592|pid:none) Ostreococcus lucimarinus CCE9901...   138   4e-31
AE016825_1644(AE016825|pid:none) Chromobacterium violaceum ATCC ...   109   1e-29
AP009552_4815(AP009552|pid:none) Microcystis aeruginosa NIES-843...   104   9e-28
protein update 2009. 6.21
PSORT 

 psg: 0.89  gvh: 0.63  alm: 0.25  top: 0.63  tms: 0.07  mit: 0.42  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.03  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VFH703 is nuc
5' end seq. ID VFH703F
5' end seq. 
>VFH703F.Seq
ACTGTTGGCCTACTGGGTTATCATCCAATGTCAGTATAAACCAACATCAATTTTAAAGAA
TTATGAAATTGGATATATTTCATCATATGAACATCCAAAATTTGTTCAAAAATCAGTATT
TCTACAAGTACATTAAAAGAGAGAGTTAGAAAACATTTTAAAGCAACCGATCAAGATCCA
CAAATGGCAGTTAGTATTTTCTCACGTTTAGCATTGGTTTATCTTTTAGTGTTTGTAACT
TACTATTTAGCACATTATACCAGTAACAATTTTTATTTAAATTGTTTCTTGGCGATCGTT
TACGCACTTTGCAACTCATTATTCTCAATGCATATGATGCATGATTCATGTCATGCTGCC
ATTTCACATTATCCAGGCGTATGGAAATGGATGGGCGCATCATTTGATTTCGTCACTGGT
GCTTCATTCCTTTCATGGTGTCATCAACATGTAATTGGTCATCATATTTACACCAATGTA
AGAAATGCCGATCCAGATCTTGGTCAAGGTGAGGTCCGATTTCCGTATTGNNNNNNNNNN
Length of 5' end seq. 540
3' end seq. ID VFH703Z
3' end seq. 
>VFH703Z.Seq
NNNNNNNNNNTATTTACACACTCAAATATNGTACTCAAGATTGGGAAGCTTTTGTAAAGN
ATGGTAAAAATGGTGCAATTCGTGTTAGTGTCGCCACAAATTTCGATAAGGCCGCTTACG
TCATTGGTAAATTGTCTTTTGTTTTCTTCCGTTTCATCCTTCCACTCCGTTATCATAGCT
TTACAGATTTAATTTGTTATTTCCTCATTGCTGAATTCGTCTTTGGTTGGTATCTCACAA
TTAATTTCCAAGTTAGTCATGTCGCTGAAGATCTCAAATTCTTTGCTACCCCTGAAAGAC
CAGATGAACCATCTCAAATCAATGAAGATTGGGCAATCCTTCAACTTANAACTACTCAAG
ATTATGGTCATGGTTCACTCCTTTGTACCTTTTTTAGTGGTTCTTTAAATCATCAAGTTG
TTCATCATTTATTCCCATCAATTGCTCAAGATTTCTACCCACAACTTGTACCAATTGTAA
AAGAAGTTTGTAAAGAACATAACATTACTTACCACATTAAACCAAACTTCACTGAAGCTA
TTATGTCACACATTAATTACCTTTACAAAATGGGTAATGATCCAGATTATGTTAAAAAAC
CATTAGCCTCAAAAGA
Length of 3' end seq. 616
Connected seq. ID VFH703P
Connected seq. 
>VFH703P.Seq
ACTGTTGGCCTACTGGGTTATCATCCAATGTCAGTATAAACCAACATCAATTTTAAAGAA
TTATGAAATTGGATATATTTCATCATATGAACATCCAAAATTTGTTCAAAAATCAGTATT
TCTACAAGTACATTAAAAGAGAGAGTTAGAAAACATTTTAAAGCAACCGATCAAGATCCA
CAAATGGCAGTTAGTATTTTCTCACGTTTAGCATTGGTTTATCTTTTAGTGTTTGTAACT
TACTATTTAGCACATTATACCAGTAACAATTTTTATTTAAATTGTTTCTTGGCGATCGTT
TACGCACTTTGCAACTCATTATTCTCAATGCATATGATGCATGATTCATGTCATGCTGCC
ATTTCACATTATCCAGGCGTATGGAAATGGATGGGCGCATCATTTGATTTCGTCACTGGT
GCTTCATTCCTTTCATGGTGTCATCAACATGTAATTGGTCATCATATTTACACCAATGTA
AGAAATGCCGATCCAGATCTTGGTCAAGGTGAGGTCCGATTTCCGTATTG----------
TATTTACACACTCAAATATNGTACTCAAGATTGGGAAGCTTTTGTAAAGNATGGTAAAAA
TGGTGCAATTCGTGTTAGTGTCGCCACAAATTTCGATAAGGCCGCTTACGTCATTGGTAA
ATTGTCTTTTGTTTTCTTCCGTTTCATCCTTCCACTCCGTTATCATAGCTTTACAGATTT
AATTTGTTATTTCCTCATTGCTGAATTCGTCTTTGGTTGGTATCTCACAATTAATTTCCA
AGTTAGTCATGTCGCTGAAGATCTCAAATTCTTTGCTACCCCTGAAAGACCAGATGAACC
ATCTCAAATCAATGAAGATTGGGCAATCCTTCAACTTANAACTACTCAAGATTATGGTCA
TGGTTCACTCCTTTGTACCTTTTTTAGTGGTTCTTTAAATCATCAAGTTGTTCATCATTT
ATTCCCATCAATTGCTCAAGATTTCTACCCACAACTTGTACCAATTGTAAAAGAAGTTTG
TAAAGAACATAACATTACTTACCACATTAAACCAAACTTCACTGAAGCTATTATGTCACA
CATTAATTACCTTTACAAAATGGGTAATGATCCAGATTATGTTAAAAAACCATTAGCCTC
AAAAGA
Length of connected seq. 1136
Full length Seq ID -
Full length Seq. -
Length of full length seq. -