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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFB504
Library AF
(Link to library)
Clone ID AFB504
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16337-1
Original site URL
Representative seq. ID AFB504P
(Link to Original site)
Representative DNA sequence 
>AFB504 (AFB504Q) /CSM/AF/AFB5-A/AFB504Q.Seq.d/
GAATNATACCATGTACAAATATTGTATNAATGAATTGTTGCATCATTTACATTATTCTGC
AAAGAATAATATCTTCTTTTGCTTCACCAATACCAGAAGTTCAATGTATAGAGCTGGACC
AGCCCTTACACTTCTCCAAACTCATTTGGAATCAATGGTGCATCATACAGACGTAAAAAT
GGAAACCACCGGTAGAGTATTCAATTTTGAAAATGAAGCATTCAATGTATTGGCATCAAA
ATTAAATGGTGCAAACTTTTCAGAAGAAGATATTGAAGTTTATTCCCACAGTTGGAAGAG
ATCATCATCCGAGGCCACCAAATTAATGAATACAATTCTTAGTACTCCACCACATAGAGT
TAAAGATACTGAATGTATTAATAACACCAGAGAAATGATCAGAAAGATGGGTACACCAAT
TCATTTCTGTTTAACTTCAATTCAAAGATTTTTATATAATATTGAACAACAAAATGAAAA
TATCAGAAAACATAAAGATGATTTAGAGAAATTGAAACCATATGTCATGGCACAACTCGA
TAAAXXXXXXXXXXACTGGCATCACACATTTGGTATCTTGGGTGGTTGTAAAGAATGTGG
TTGTTCTTATAAAGATCATTCAGTGTCTGATATCGTTCATGTTAAAGAAGCAATTCAAGT
CATTGATGAAGGTATCAAAGGTAAAATCCTTTCCAAAGAAGAACTCACTGCTGTCGCCAA
TAGAAGAGTTAAAGAGAATTTAGAACTCAAAGAGAAATATTCCCAAGAATTTGAGGTTTT
ATCAAATGCATTTGCCAAATTTGCAATCTTTGTAGCTCATAATTCATTGGTTGCTGTTAA
TCACGACTATGCAGAATACCTCCAATTGGAAATTAAAAACCTTAAAGAAAGCCCAAATGT
TAAAGATAGAGAGAAACAATTAGAAAAATTAAATGAAATCCTTAAATCTCATCAAATTGA
ACTTGATACTTTTGAAGCCGCCCGTTCAAAAGAAAAAGAAGGTCTTTCACCACACGATAT
AAATCTCCTTGGTGAAGAAGTTTTATCACTTGAAATCTCTGGTAGAATCATTAAAGATAT
TCAAAGTCATAATNAAAAACAAAAATATGCTTCATCAAAAAAAGTTAAAAATGTTAAAAT
TACATTAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
NXTMYKYCXNELLHHLHYSAKNNIFFCFTNTRSSMYRAGPALTLLQTHLESMVHHTDVKM
ETTGRVFNFENEAFNVLASKLNGANFSEEDIEVYSHSWKRSSSEATKLMNTILSTPPHRV
KDTECINNTREMIRKMGTPIHFCLTSIQRFLYNIEQQNENIRKHKDDLEKLKPYVMAQLD
K---

---WHHTFGILGGCKECGCSYKDHSVSDIVHVKEAIQVIDEGIKGKILSKEELTAVANRR
VKENLELKEKYSQEFEVLSNAFAKFAIFVAHNSLVAVNHDYAEYLQLEIKNLKESPNVKD
REKQLEKLNEILKSHQIELDTFEAARSKEKEGLSPHDINLLGEEVLSLEISGRIIKDIQS
HNXKQKYASSKKVKNVKITL
Translated Amino Acid sequence (All Frames) 
Frame A:
exyhvqilyx*ivasftlfcke*yllllhqyqkfnv*swtspytspnsfgingasyrrkn
gnhr*siqf*k*siqcigikikwcklfrrry*slfpqleeiiirghqineyns*ystt*s
*ry*my**hqrndqkdgytnsflfnfnskifi*y*ttk*kyqkt*r*freietichgttr
*---

---tgithlvswvvvknvvvlikiiqclisfmlkkqfkslmkvskvksfpkknsllspie
elkri*nskrnipknlrfyqmhlpnlqsl*liihwlllittmqntsnwklktlkkaqmlk
iernn*kn*mkslnliklnlillkppvqkkkkvfhhti*islvkkfyhlkslveslkifk
vixknknmlhqkklkmlklh*

Frame B:
NXTMYKYCXNELLHHLHYSAKNNIFFCFTNTRSSMYRAGPALTLLQTHLESMVHHTDVKM
ETTGRVFNFENEAFNVLASKLNGANFSEEDIEVYSHSWKRSSSEATKLMNTILSTPPHRV
KDTECINNTREMIRKMGTPIHFCLTSIQRFLYNIEQQNENIRKHKDDLEKLKPYVMAQLD
K---

---lashiwylgwl*rmwlfl*rsfsv*yrsc*rsnssh**ryqr*npfqrrthccrq*k
s*refrtqreifpri*gfikcicqicnlcss*figcc*srlcrippign*kp*rkpkc*r
*retirkik*np*issn*t*yf*srpfkrkrrsfttrykspw*rsfit*nlw*nh*rysk
s*xktkicfikks*kc*nyi

Frame C:
xipctnivxmncciiyiilqriissfaspipevqcieldqplhfskliwnqwciiqt*kw
kppveysilkmkhsmywhqn*mvqtfqkkilkfiptvgrdhhprppn**iqflvlhhiel
kilnvlitpek*serwvhqfisv*lqfkdfyiilnnkmkisenikmi*rn*nhmswhnsi
---

---WHHTFGILGGCKECGCSYKDHSVSDIVHVKEAIQVIDEGIKGKILSKEELTAVANRR
VKENLELKEKYSQEFEVLSNAFAKFAIFVAHNSLVAVNHDYAEYLQLEIKNLKESPNVKD
REKQLEKLNEILKSHQIELDTFEAARSKEKEGLSPHDINLLGEEVLSLEISGRIIKDIQS
HNXKQKYASSKKVKNVKITL
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHC723 (VHC723Q) /CSM/VH/VHC7-A/VHC723Q.Seq.d/                       1068   0.0
SFK866 (SFK866Q) /CSM/SF/SFK8-C/SFK866Q.Seq.d/                       1068   0.0
SFH491 (SFH491Q) /CSM/SF/SFH4-D/SFH491Q.Seq.d/                       1068   0.0
SFG571 (SFG571Q) /CSM/SF/SFG5-C/SFG571Q.Seq.d/                       1068   0.0
SFF558 (SFF558Q) /CSM/SF/SFF5-C/SFF558Q.Seq.d/                       1068   0.0
CHD529 (CHD529Q) /CSM/CH/CHD5-B/CHD529Q.Seq.d/                       1068   0.0
AHO519 (AHO519Q) /CSM/AH/AHO5-A/AHO519Q.Seq.d/                       1068   0.0
AHJ858 (AHJ858Q) /CSM/AH/AHJ8-C/AHJ858Q.Seq.d/                       1068   0.0
AHD889 (AHD889Q) /CSM/AH/AHD8-D/AHD889Q.Seq.d/                       1068   0.0
AHA602 (AHA602Q) /CSM/AH/AHA6-A/AHA602Q.Seq.d/                       1068   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AP003190|AP003190.2 Clostridium perfringens str. 13 DNA, complete genome, section 6/10.    34  0.071  11
Z81498|Z81498.1 Caenorhabditis elegans cosmid F11A6.              40  0.096  3
AC117790|AC117790.9 Mus musculus clone RP24-548O2, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    36  0.46  6
AE010553|AE010553.1 Fusobacterium nucleatum subsp. nucleatum ATCC 25586, section 95 of 197 of the complete genome.    30  0.65  6
AC116157|AC116157.4 Homo sapiens chromosome 15, clone RP11-1006B19, complete sequence.    36  0.67  6
AB063522|AB063522.2 Wigglesworthia brevipalpis DNA, complete genome, section 2/2.    34  0.73  9
AC110537|AC110537.5 Mus musculus clone RP23-44A4, WORKING DRAFT SEQUENCE, 13 ordered pieces.    36  0.78  7
AF354651|AF354651.1 Streptococcus dysgalactiae Mig precursor, gene, complete cds.    46  0.96  1
Z29666|Z29666.1 S.dysgalactiae SC1 mig gene for surface protein.    46  0.96  1
I08537|I08537.1 Sequence 4 from Patent WO 8705025.                46  0.96  1
dna update 2003. 7.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB120174_1(AB120174|pid:none) Dictyostelium discoideum DD8-14 mR...   727   0.0
AC114263_3(AC114263|pid:none) Dictyostelium discoideum chromosom...   177   8e-43
T28676(T28676;A45521)rhoptry protein - Plasmodium yoelii (fragme...    46   0.002
EU159424_1(EU159424|pid:none) Triticum turgidum haplotype B DNA ...    42   0.029
EU159420_1(EU159420|pid:none) Triticum turgidum haplotype B DNA ...    42   0.029
EU159423_1(EU159423|pid:none) Triticum turgidum haplotype A DNA ...    42   0.029
EU159417_1(EU159417|pid:none) Triticum monococcum haplotype A DN...    42   0.029
EU159418_1(EU159418|pid:none) Aegilops tauschii haplotype D DNA ...    42   0.029
EU159419_1(EU159419|pid:none) Triticum turgidum haplotype A DNA ...    42   0.038
CR954211_349(CR954211|pid:none) Ostreococcus tauri strain OTTH05...    42   0.038
protein update 2009. 3.27
PSORT 

 psg: 0.75  gvh: 0.35  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.38  mip: 0.07
 nuc: 0.02  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for AFB504 is cyt
5' end seq. ID AFB504F
5' end seq. 
>AFB504F.Seq
GAATNATACCATGTACAAATATTGTATNAATGAATTGTTGCATCATTTACATTATTCTGC
AAAGAATAATATCTTCTTTTGCTTCACCAATACCAGAAGTTCAATGTATAGAGCTGGACC
AGCCCTTACACTTCTCCAAACTCATTTGGAATCAATGGTGCATCATACAGACGTAAAAAT
GGAAACCACCGGTAGAGTATTCAATTTTGAAAATGAAGCATTCAATGTATTGGCATCAAA
ATTAAATGGTGCAAACTTTTCAGAAGAAGATATTGAAGTTTATTCCCACAGTTGGAAGAG
ATCATCATCCGAGGCCACCAAATTAATGAATACAATTCTTAGTACTCCACCACATAGAGT
TAAAGATACTGAATGTATTAATAACACCAGAGAAATGATCAGAAAGATGGGTACACCAAT
TCATTTCTGTTTAACTTCAATTCAAAGATTTTTATATAATATTGAACAACAAAATGAAAA
TATCAGAAAACATAAAGATGATTTAGAGAAATTGAAACCATATGTCATGGCACAACTCGA
TAAA----------
Length of 5' end seq. 544
3' end seq. ID AFB504Z
3' end seq. 
>AFB504Z.Seq
----------ACTGGCATCACACATTTGGTATCTTGGGTGGTTGTAAAGAATGTGGTTGT
TCTTATAAAGATCATTCAGTGTCTGATATCGTTCATGTTAAAGAAGCAATTCAAGTCATT
GATGAAGGTATCAAAGGTAAAATCCTTTCCAAAGAAGAACTCACTGCTGTCGCCAATAGA
AGAGTTAAAGAGAATTTAGAACTCAAAGAGAAATATTCCCAAGAATTTGAGGTTTTATCA
AATGCATTTGCCAAATTTGCAATCTTTGTAGCTCATAATTCATTGGTTGCTGTTAATCAC
GACTATGCAGAATACCTCCAATTGGAAATTAAAAACCTTAAAGAAAGCCCAAATGTTAAA
GATAGAGAGAAACAATTAGAAAAATTAAATGAAATCCTTAAATCTCATCAAATTGAACTT
GATACTTTTGAAGCCGCCCGTTCAAAAGAAAAAGAAGGTCTTTCACCACACGATATAAAT
CTCCTTGGTGAAGAAGTTTTATCACTTGAAATCTCTGGTAGAATCATTAAAGATATTCAA
AGTCATAATNAAAAACAAAAATATGCTTCATCAAAAAAAGTTAAAAATGTTAAAATTACA
TTAA
Length of 3' end seq. 594
Connected seq. ID AFB504P
Connected seq. 
>AFB504P.Seq
GAATNATACCATGTACAAATATTGTATNAATGAATTGTTGCATCATTTACATTATTCTGC
AAAGAATAATATCTTCTTTTGCTTCACCAATACCAGAAGTTCAATGTATAGAGCTGGACC
AGCCCTTACACTTCTCCAAACTCATTTGGAATCAATGGTGCATCATACAGACGTAAAAAT
GGAAACCACCGGTAGAGTATTCAATTTTGAAAATGAAGCATTCAATGTATTGGCATCAAA
ATTAAATGGTGCAAACTTTTCAGAAGAAGATATTGAAGTTTATTCCCACAGTTGGAAGAG
ATCATCATCCGAGGCCACCAAATTAATGAATACAATTCTTAGTACTCCACCACATAGAGT
TAAAGATACTGAATGTATTAATAACACCAGAGAAATGATCAGAAAGATGGGTACACCAAT
TCATTTCTGTTTAACTTCAATTCAAAGATTTTTATATAATATTGAACAACAAAATGAAAA
TATCAGAAAACATAAAGATGATTTAGAGAAATTGAAACCATATGTCATGGCACAACTCGA
TAAA----------ACTGGCATCACACATTTGGTATCTTGGGTGGTTGTAAAGAATGTGG
TTGTTCTTATAAAGATCATTCAGTGTCTGATATCGTTCATGTTAAAGAAGCAATTCAAGT
CATTGATGAAGGTATCAAAGGTAAAATCCTTTCCAAAGAAGAACTCACTGCTGTCGCCAA
TAGAAGAGTTAAAGAGAATTTAGAACTCAAAGAGAAATATTCCCAAGAATTTGAGGTTTT
ATCAAATGCATTTGCCAAATTTGCAATCTTTGTAGCTCATAATTCATTGGTTGCTGTTAA
TCACGACTATGCAGAATACCTCCAATTGGAAATTAAAAACCTTAAAGAAAGCCCAAATGT
TAAAGATAGAGAGAAACAATTAGAAAAATTAAATGAAATCCTTAAATCTCATCAAATTGA
ACTTGATACTTTTGAAGCCGCCCGTTCAAAAGAAAAAGAAGGTCTTTCACCACACGATAT
AAATCTCCTTGGTGAAGAAGTTTTATCACTTGAAATCTCTGGTAGAATCATTAAAGATAT
TCAAAGTCATAATNAAAAACAAAAATATGCTTCATCAAAAAAAGTTAAAAATGTTAAAAT
TACATTAA
Length of connected seq. 1138
Full length Seq ID -
Full length Seq. -
Length of full length seq. -