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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFH648
Library VF
(Link to library)
Clone ID VFH648
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13493-1|Contig-U16091-1
Original site URL
Representative seq. ID VFH648P
(Link to Original site)
Representative DNA sequence 
>VFH648 (VFH648Q) /CSM/VF/VFH6-B/VFH648Q.Seq.d/
ACTGTTGGCCTACTGGGCGAGCTGTGGATATATTGATATTTATTATTATATATATAAATT
ATTATTTATCTTTTAATTAATAATTATATATTTAATTTTATCTTTAAAAAAAAAAAAAAA
ACCAAAAAAANTAAAAAAATTTTAAAAACCAAAAAAAAAAXXXXXXXXXXTATCGTTATG
AAAGACGACAAAGCCACCACCAGCAAAGGTTTTGGTTTCGTTTGTTACACCGCACCAGAT
GAGGCCACTCGTGCCGTCACCGAAATGAACGGTCGTATGATTGGTACCAAGCCATTGTAC
GTTGCTTTAGCTCAACGTAAAGATATTCGTCGTGCTCAACTCGAAATGCAACACCAACAA
AAATTCAAAACTGGTATCCGTCAACAAATGCCACCAACCTACGGTAGTGGTCCAGTTTTC
TTCACTCCAGCCCCAGTCAACCCACAAGTTGTCTACCAACAAATGATGCCACGTCCACGT
AACTGGAACGGTCAACCAGTAGGTGTCCCACAAGGTCAATACGCCAATATGAACTATGCC
CGTGGTCAACCACGTCAAAATGGTCCACGTCAAAACGGTGGTCAACCACGTCAAAACGGT
CCACGTCCAGACGTTTCAGGTGCTCAACCAATTCCAGTTCAACAACAAACCACTTTGGAT
GTCGCTCA
sequence update 2001.11.22
Translated Amino Acid sequence 
tvgllgelwiy*ylllyi*IIIYLLINNYIFNFIFKKKKKTKKXKKILKTKKK---

---IVMKDDKATTSKGFGFVCYTAPDEATRAVTEMNGRMIGTKPLYVALAQRKDIRRAQL
EMQHQQKFKTGIRQQMPPTYGSGPVFFTPAPVNPQVVYQQMMPRPRNWNGQPVGVPQGQY
ANMNYARGQPRQNGPRQNGGQPRQNGPRPDVSGAQPIPVQQQTTLDVA
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllgelwiy*ylllyi*IIIYLLINNYIFNFIFKKKKKTKKXKKILKTKKK---

---yryerrqshhqqrfwfrllhrtr*ghscrhrnersydwyqaivrcfsst*rysscst
rnatptkiqnwypstnatnlr*wssflhsspsqptsclptndatst*lerstsrcptrsi
rqyelcpwsttskwstskrwsttskrstsrrfrcstnsssttnhfgcrs

Frame B:
llaywascgyidiyyyiykllfif*liiiylilslkkkkkpkkxkkf*kpkkk---

---IVMKDDKATTSKGFGFVCYTAPDEATRAVTEMNGRMIGTKPLYVALAQRKDIRRAQL
EMQHQQKFKTGIRQQMPPTYGSGPVFFTPAPVNPQVVYQQMMPRPRNWNGQPVGVPQGQY
ANMNYARGQPRQNGPRQNGGQPRQNGPRPDVSGAQPIPVQQQTTLDVA

Frame C:
cwptgravdilifiiiyinyylsfn**lyi*fyl*kkkknqkx*knfknqkk---

---sl*kttkpppakvlvsfvtphqmrplvpspk*tvv*lvpshctll*lnvkifvvlns
kcntnknsklvsvnkchqptvvvqfsslqpqsthklstnk*chvhvtgtvnq*vshkvnt
pi*tmpvvnhvkmvhvktvvnhvktvhvqtfqvlnqfqfnnkplwmsl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFH648 (VFH648Q) /CSM/VF/VFH6-B/VFH648Q.Seq.d/                        987   0.0
VFI195 (VFI195Q) /CSM/VF/VFI1-D/VFI195Q.Seq.d/                        821   0.0
SLA837 (SLA837Q) /CSM/SL/SLA8-B/SLA837Q.Seq.d/                        809   0.0
SLB221 (SLB221Q) /CSM/SL/SLB2-A/SLB221Q.Seq.d/                        660   0.0
SLE325 (SLE325Q) /CSM/SL/SLE3-B/SLE325Q.Seq.d/                        648   0.0
VSG606 (VSG606Q) /CSM/VS/VSG6-A/VSG606Q.Seq.d/                        646   0.0
FC-AP19 (FC-AP19Q) /CSM/FC/FC-AP/FC-AP19Q.Seq.d/                      615   e-175
FC-269 (FC-269Q) /CSM/CG/CG-FC/FC-269Q.Seq.d/                         615   e-175
SLE683 (SLE683Q) /CSM/SL/SLE6-D/SLE683Q.Seq.d/                        579   e-164
VFL720 (VFL720Q) /CSM/VF/VFL7-A/VFL720Q.Seq.d/                        565   e-160
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AX536940|AX536940.1 Sequence 541 from Patent WO02064766.           50   1e-06  2
BG688103|BG688103.1 335557 BARC 5BOV Bos taurus cDNA 5', mRNA sequence.    44   2e-04  2
BG938001|BG938001.1 1Abo08B05 Bovine Abomasum cDNA Library Bos taurus cDNA 5', mRNA sequence.    44   2e-04  2
CB062515|CB062515.1 4012285 BARC-EMBRAPA 351BOV Bos indicus cDNA clone 351BOV_1C03 Unknown, mRNA sequence.    44   3e-04  2
AW462652|AW462652.1 BP230010A10B12 Soares normalized bovine placenta Bos taurus cDNA clone BP230010A10B12 5', mRNA sequence.    44   3e-04  2
CB286131|CB286131.1 CMD31_B07_58 UMNMPM3 Sus scrofa cDNA clone PPSUBLIB_17B07 5', mRNA sequence.    44   3e-04  2
U83077|U83077.1 U83077 Bovine ovary cDNA Bos taurus cDNA clone 0815, mRNA sequence.    44   3e-04  2
AW463512|AW463512.1 BP230013A10B6 Soares normalized bovine placenta Bos taurus cDNA clone BP230013A10B6 5', mRNA sequence.    44   3e-04  2
BF043813|BF043813.1 BP250024A20H5 Soares normalized bovine placenta Bos taurus cDNA clone BP250024A20H5 5', mRNA sequence.    44   4e-04  2
CB466045|CB466045.1 728128 MARC 6BOV Bos taurus cDNA 5', mRNA sequence.    44   4e-04  2
dna update 2004. 1.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(A5DM21) RecName: Full=Polyadenylate-binding protein, cytoplasmi...   110   5e-23
(Q5KBW2) RecName: Full=Polyadenylate-binding protein, cytoplasmi...   109   8e-23
FM992688_313(FM992688|pid:none) Candida dubliniensis CD36 chromo...   107   2e-22
(Q6CDH3) RecName: Full=Polyadenylate-binding protein, cytoplasmi...   107   3e-22
(A5DW14) RecName: Full=Polyadenylate-binding protein, cytoplasmi...   107   3e-22
DQ646407_1(DQ646407|pid:none) Bombyx mori poly A binding protein...   106   7e-22
(Q5AI15) RecName: Full=Polyadenylate-binding protein, cytoplasmi...   105   1e-21
(P31209) RecName: Full=Polyadenylate-binding protein, cytoplasmi...   101   2e-20
AL032631_3(AL032631|pid:none) Caenorhabditis elegans YAC Y106G6H...    99   9e-20
AL032631_5(AL032631|pid:none) Caenorhabditis elegans YAC Y106G6H...    99   9e-20
protein update 2009. 6.21
PSORT 

 psg: 0.88  gvh: 0.37  alm: 0.36  top: 1.13  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.36  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.33
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: nuclear
	 20.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for VFH648 is nuc
5' end seq. ID VFH648F
5' end seq. 
>VFH648F.Seq
ACTGTTGGCCTACTGGGCGAGCTGTGGATATATTGATATTTATTATTATATATATAAATT
ATTATTTATCTTTTAATTAATAATTATATATTTAATTTTATCTTTAAAAAAAAAAAAAAA
ACCAAAAAAANTAAAAAAATTTTAAAAACCAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 170
3' end seq. ID VFH648Z
3' end seq. 
>VFH648Z.Seq
NNNNNNNNNNTATCGTTATGAAAGACGACAAAGCCACCACCAGCAAAGGTTTTGGTTTCG
TTTGTTACACCGCACCAGATGAGGCCACTCGTGCCGTCACCGAAATGAACGGTCGTATGA
TTGGTACCAAGCCATTGTACGTTGCTTTAGCTCAACGTAAAGATATTCGTCGTGCTCAAC
TCGAAATGCAACACCAACAAAAATTCAAAACTGGTATCCGTCAACAAATGCCACCAACCT
ACGGTAGTGGTCCAGTTTTCTTCACTCCAGCCCCAGTCAACCCACAAGTTGTCTACCAAC
AAATGATGCCACGTCCACGTAACTGGAACGGTCAACCAGTAGGTGTCCCACAAGGTCAAT
ACGCCAATATGAACTATGCCCGTGGTCAACCACGTCAAAATGGTCCACGTCAAAACGGTG
GTCAACCACGTCAAAACGGTCCACGTCCAGACGTTTCAGGTGCTCAACCAATTCCAGTTC
AACAACAAACCACTTTGGATGTCGCTCA
Length of 3' end seq. 508
Connected seq. ID VFH648P
Connected seq. 
>VFH648P.Seq
ACTGTTGGCCTACTGGGCGAGCTGTGGATATATTGATATTTATTATTATATATATAAATT
ATTATTTATCTTTTAATTAATAATTATATATTTAATTTTATCTTTAAAAAAAAAAAAAAA
ACCAAAAAAANTAAAAAAATTTTAAAAACCAAAAAAAAAA----------TATCGTTATG
AAAGACGACAAAGCCACCACCAGCAAAGGTTTTGGTTTCGTTTGTTACACCGCACCAGAT
GAGGCCACTCGTGCCGTCACCGAAATGAACGGTCGTATGATTGGTACCAAGCCATTGTAC
GTTGCTTTAGCTCAACGTAAAGATATTCGTCGTGCTCAACTCGAAATGCAACACCAACAA
AAATTCAAAACTGGTATCCGTCAACAAATGCCACCAACCTACGGTAGTGGTCCAGTTTTC
TTCACTCCAGCCCCAGTCAACCCACAAGTTGTCTACCAACAAATGATGCCACGTCCACGT
AACTGGAACGGTCAACCAGTAGGTGTCCCACAAGGTCAATACGCCAATATGAACTATGCC
CGTGGTCAACCACGTCAAAATGGTCCACGTCAAAACGGTGGTCAACCACGTCAAAACGGT
CCACGTCCAGACGTTTCAGGTGCTCAACCAATTCCAGTTCAACAACAAACCACTTTGGAT
GTCGCTCA
Length of connected seq. 658
Full length Seq ID -
Full length Seq. -
Length of full length seq. -