>VFH580 (VFH580Q) /CSM/VF/VFH5-D/VFH580Q.Seq.d/
XXXXXXXXXXCTAATTATATTCCCAGCAATTATTAAAATGTTGGAAAATGATAATTCACC
AGGGTCAAGTTTGTCAAAAACTTAATCTTTGTCCAGCACCAACTCCAACTTCAACACCTT
CAACAATCAAGATTGATGTGAATATTAATAAATGTGAAATTTGTGAAACTGTAATTGGTT
TTGCTGAAAAATATTTAGGTTCAGCATTAGATTCTCAAACTGTAAAGACATTCCTCCAAA
ATGAATGTAACAAACTTCCAGGTGTTGTTGGTGATGTTTGTAATGAAGTTGTAAATGAAT
ATCTTGAGTTTGCAGTCACTCAACTCGAAGCTAAATTTAACCCAACTGAAATTTGTCAAT
TATTTTCATTCTGTACTGCTGGATCTGAATCATCAGATGCACCTGAAATCAAAATTAATA
ACAACTCATATATTGATTGTCAAATTTGTGAAGTTTTAGCAAATTTCGTAGTCTATGATA
AGAATTATACAACTCATTTCGAAGATAAACTCAATAAATTATGTAGTTGTTTATCTCGTA
GTTTAATTGAACCATGTCTTAACTTTGTTTCACAATATGGTTCTGAT

---*LYSQQLLKCWKMIIHQGQVCQKLNLCPAPTPTSTPSTIKIDVNINKCEICETVIGF
AEKYLGSALDSQTVKTFLQNECNKLPGVVGDVCNEVVNEYLEFAVTQLEAKFNPTEICQL
FSFCTAGSESSDAPEIKINNNSYIDCQICEVLANFVVYDKNYTTHFEDKLNKLCSCLSRS
LIEPCLNFVSQYGSD

Frame A:
---*LYSQQLLKCWKMIIHQGQVCQKLNLCPAPTPTSTPSTIKIDVNINKCEICETVIGF
AEKYLGSALDSQTVKTFLQNECNKLPGVVGDVCNEVVNEYLEFAVTQLEAKFNPTEICQL
FSFCTAGSESSDAPEIKINNNSYIDCQICEVLANFVVYDKNYTTHFEDKLNKLCSCLSRS
LIEPCLNFVSQYGSD


Frame B:
---nyipsny*nvgk**ftrvkfvknlifvqhqlqlqhlqqsrlm*ilinvkfvkl*lvl
lkni*vqh*ilkl*rhsskmnvtnfqvllvmfvmkl*mnilslqslnsklnltqlkfvny
fhsvlldlnhqmhlksklitthilivkfvkf*qis*smiriiqliskinsinyvvvylvv
*lnhvltlfhnmvl


Frame C:
---iifpaiikmlendnspgsslskt*slsstnsnfntfnnqd*cey**m*nl*ncnwfc
*kifrfsirfsnckdippk*m*qtsrccw*cl**sck*is*vcshstrs*i*pn*nlsii
filycwi*iirct*nqn**qliy*lsnl*sfskfrsl**elynsfrr*tq*im*lfis*f
n*tms*lcftiwf*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFH580 (VFH580Q) /CSM/VF/VFH5-D/VFH580Q.Seq.d/                       1144   0.0
VFJ635 (VFJ635Q) /CSM/VF/VFJ6-B/VFJ635Q.Seq.d/                       1096   0.0
VFD617 (VFD617Q) /CSM/VF/VFD6-A/VFD617Q.Seq.d/                       1092   0.0
VFB595 (VFB595Q) /CSM/VF/VFB5-D/VFB595Q.Seq.d/                       1088   0.0
VFG662 (VFG662Q) /CSM/VF/VFG6-C/VFG662Q.Seq.d/                       1082   0.0
VFB732 (VFB732Q) /CSM/VF/VFB7-B/VFB732Q.Seq.d/                       1080   0.0
VFM205 (VFM205Q) /CSM/VF/VFM2-A/VFM205Q.Seq.d/                       1074   0.0
VFH555 (VFH555Q) /CSM/VF/VFH5-C/VFH555Q.Seq.d/                       1047   0.0
VFA464 (VFA464Q) /CSM/VF/VFA4-C/VFA464Q.Seq.d/                       1031   0.0
VFI241 (VFI241Q) /CSM/VF/VFI2-B/VFI241Q.Seq.d/                       1027   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U66367|U66367.1 Dictyostelium discoideum SapA (sapA) mRNA, partial cds.  1025   0.0    2
AR300198|AR300198.1 Sequence 1 from patent US 6537773.             36   0.005  15
AP004171|AP004171.1 Mycoplasma penetrans genomic DNA, complete sequence, section 2/5.    36   0.005  10
AC010477|AC010477.8 Homo sapiens chromosome 5 clone CTD-2313D3, complete sequence.    42   0.007  7
AC053535|AC053535.4 Homo sapiens chromosome 8 clone RP11-745D6, WORKING DRAFT SEQUENCE, 10 unordered pieces.    40   0.089  5
AL031655|AL031655.8 Human DNA sequence from clone RP1-134N8 on chromosome 20p12.  Contains STSs, GSSs and a CpG island.    36   0.14   7
AC114964|AC114964.2 Homo sapiens chromosome 5 clone RP11-275B7, complete sequence.    42   0.22   7
AE014825|AE014825.1 Plasmodium falciparum 3D7 chromosome 14 section 10 of 13 of the complete sequence.    34   0.45   8
BX248585|BX248585.1 Blochmannia floridanus complete genome; segment 2/3.    32   0.46   10
AP000900|AP000900.3 Homo sapiens genomic DNA, chromosome 18p11.3 clone:RP11-683J11, WORKING DRAFT SEQUENCE, 20 unordered pieces.    36   0.46   5

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U66367_1(U66367|pid:none) Dictyostelium discoideum SapA (sapA) m...   334   1e-90
BC054988_1(BC054988|pid:none) Xenopus laevis hypothetical protei...    71   2e-11
BC080997_1(BC080997|pid:none) Xenopus laevis MGC80725 protein, m...    68   2e-10
DQ062781_1(DQ062781|pid:none) Drosophila simulans saposin-relate...    66   8e-10
AF108656_1(AF108656|pid:none) Gallus gallus prosaposin mRNA, com...    63   7e-09
AF162129_1(AF162129|pid:none) Drosophila melanogaster ecdysone d...    63   7e-09
(O13035) RecName: Full=Proactivator polypeptide; Contains:   Rec...    63   7e-09
AF175768_1(AF175768|pid:none) Dictyostelium discoideum SAPLIP C ...    59   1e-07
CR456746_1(CR456746|pid:none) Homo sapiens full open reading fra...    57   3e-07
AK223290_1(AK223290|pid:none) Homo sapiens mRNA for prosaposin (...    57   3e-07

 psg: 0.77  gvh: 0.40  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.37  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: mitochondrial
	 28.0 %: nuclear
	 24.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VFH580 is mit

>VFH580Z.Seq
NNNNNNNNNNCTAATTATATTCCCAGCAATTATTAAAATGTTGGAAAATGATAATTCACC
AGGGTCAAGTTTGTCAAAAACTTAATCTTTGTCCAGCACCAACTCCAACTTCAACACCTT
CAACAATCAAGATTGATGTGAATATTAATAAATGTGAAATTTGTGAAACTGTAATTGGTT
TTGCTGAAAAATATTTAGGTTCAGCATTAGATTCTCAAACTGTAAAGACATTCCTCCAAA
ATGAATGTAACAAACTTCCAGGTGTTGTTGGTGATGTTTGTAATGAAGTTGTAAATGAAT
ATCTTGAGTTTGCAGTCACTCAACTCGAAGCTAAATTTAACCCAACTGAAATTTGTCAAT
TATTTTCATTCTGTACTGCTGGATCTGAATCATCAGATGCACCTGAAATCAAAATTAATA
ACAACTCATATATTGATTGTCAAATTTGTGAAGTTTTAGCAAATTTCGTAGTCTATGATA
AGAATTATACAACTCATTTCGAAGATAAACTCAATAAATTATGTAGTTGTTTATCTCGTA
GTTTAATTGAACCATGTCTTAACTTTGTTTCACAATATGGTTCTGAT