>VFH554 (VFH554Q) /CSM/VF/VFH5-C/VFH554Q.Seq.d/
AGAGTTAGAAGACTCGAAACTGNNTGGGANGGTTTATACAAAAAAAATNAATTAGAGGNT
NCTGNCATTNATACACTGGNCAAGAAGCTGNNNGNGCAGGNNNAGAAAGNGCAANNACTA
AAGAGGANCATATNATNACAGNATACAGAGANCACACTNATANGNNANNAAGAGGAGCAA
CACCAGAAGAAANNNNNGCAGAANNANNAANGAAAGAAACNGGNNGNNCAAAGGGNAAAG
GNGGNNNAANGCANANGNNNACAAAGAANNNNNANGGNGGAANGGNANNGNNGGNGCNNA
AXXXXXXXXXX

RVRRLETXWXGLYKKNXLEXXXIXTLXKKLXXQXXKXQXLKRXIXXQXTEXTLIXXXEEQ
HQKKXXQXXXXKKXXXQRXKXXXXXXXQRXXXXEXXXXXX---

Frame A:
RVRRLETXWXGLYKKNXLEXXXIXTLXKKLXXQXXKXQXLKRXIXXQXTEXTLIXXXEEQ
HQKKXXQXXXXKKXXXQRXKXXXXXXXQRXXXXEXXXXXX---


Frame B:
eledsklxgxvytkkxn*rxlxxihwxrsxxxrxrkxxx*rgxyxxxiqrxhxxxxkrsn
trrxxxrxxxernxxxkgxrxxxaxxxkexxxxxgxxga---


Frame C:
s*ktrnxxgxfiqkkxirgxxhxytgqeaxxagxexaxtkexhxxtxyrxhtxxxxrgat
peexxaexxxketgxxkgkggxxxxxtkxxxggxxxxxx---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFH554 (VFH554Q) /CSM/VF/VFH5-C/VFH554Q.Seq.d/                        157   1e-37
VFM538 (VFM538Q) /CSM/VF/VFM5-B/VFM538Q.Seq.d/                        133   2e-30
VHC605 (VHC605Q) /CSM/VH/VHC6-A/VHC605Q.Seq.d/                        123   2e-27
VFN847 (VFN847Q) /CSM/VF/VFN8-B/VFN847Q.Seq.d/                        123   2e-27
VFN280 (VFN280Q) /CSM/VF/VFN2-D/VFN280Q.Seq.d/                        123   2e-27
VFN180 (VFN180Q) /CSM/VF/VFN1-D/VFN180Q.Seq.d/                        123   2e-27
VFM688 (VFM688Q) /CSM/VF/VFM6-D/VFM688Q.Seq.d/                        123   2e-27
VFM660 (VFM660Q) /CSM/VF/VFM6-C/VFM660Q.Seq.d/                        123   2e-27
VFL810 (VFL810Q) /CSM/VF/VFL8-A/VFL810Q.Seq.d/                        123   2e-27
VFL647 (VFL647Q) /CSM/VF/VFL6-B/VFL647Q.Seq.d/                        123   2e-27

 ***** No hits found ******

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Hits to DB: 669,006
Number of Sequences: 31254990
Number of extensions: 669006
Number of successful extensions: 29886
Number of sequences better than 10.0: 0
length of query: 311
length of database: T,753,383,974
effective HSP length: 22
effective length of query: 289
effective length of database: T,065,774,194
effective search space: 10423008742066
effective search space used: 10423008742066
T: 0
A: 0
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)

ANTI-DNA BLAST search was processed by blast@nig.ac.jp, National Institute of Genetics, Japan.

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54C70) RecName: Full=Pyruvate dehydrogenase E1 component subun...    89   3e-18
BT059393_1(BT059393|pid:none) Salmo salar clone ssal-rgf-519-230...    63   3e-09
BT046034_1(BT046034|pid:none) Salmo salar clone ssal-rgf-539-340...    63   4e-09
AY623916_1(AY623916|pid:none) Euplotes sp. BB-2004 pyruvate dehy...    62   5e-09
AY623918_1(AY623918|pid:none) Nyctotherus ovalis pyruvate dehydr...    62   6e-09
AM494955_140(AM494955|pid:none) Leishmania braziliensis chromoso...    62   6e-09
AY623923_1(AY623923|pid:none) Nyctotherus ovalis pyruvate dehydr...    62   8e-09
AL928875_10(AL928875|pid:none) Zebrafish DNA sequence from clone...    61   1e-08
U56388_1(U56388|pid:none) Trypanosoma cruzi putative pyruvate de...    61   1e-08
CR761539_1(CR761539|pid:none) Xenopus tropicalis finished cDNA, ...    60   3e-08

 psg: 0.73  gvh: 0.41  alm: 0.64  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 32.0 %: cytoplasmic
	 24.0 %: mitochondrial
	  8.0 %: cytoskeletal

>> prediction for VFH554 is nuc

>VFH554F.Seq
AGAGTTAGAAGACTCGAAACTGNNTGGGANGGTTTATACAAAAAAAATNAATTAGAGGNT
NCTGNCATTNATACACTGGNCAAGAAGCTGNNNGNGCAGGNNNAGAAAGNGCAANNACTA
AAGAGGANCATATNATNACAGNATACAGAGANCACACTNATANGNNANNAAGAGGAGCAA
CACCAGAAGAAANNNNNGCAGAANNANNAANGAAAGAAACNGGNNGNNCAAAGGGNAAAG
GNGGNNNAANGCANANGNNNACAAAGAANNNNNANGGNGGAANGGNANNGNNGGNGCNNA
ANNNNNNNNNN