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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFH545
Library VF
(Link to library)
Clone ID VFH545
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12238-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VFH545 (VFH545Q) /CSM/VF/VFH5-B/VFH545Q.Seq.d/
XXXXXXXXXXAAAGATATCATTGCATTGGGTTTTGATATTCAAAAAACTTTTATTTTTTC
AAATTTAGAATATATTCATCATCTTTATCCAAATGTACTTAAAATTGCAAGATGTGTTAA
TTTAAATCAAATTCAAAATATTTTTGGATTTAAAGAATCAGATGCAATTGGTAAATTTAC
ATTTCCACCAGTTCAAGCAGCACCATGTTTTCCAGATTCATTCCCACATATTTTCCCATT
GAATGATCCAGAGATTAAGAATATTCGTTGTTTAATTCCATGTGCAATTGATCAAGATCC
ATACTTTAGAATGACTCGTGATATTGCACATAGAGTTGGTCATCAAAAACCAGCACTTAT
TCATAGTAAATTCTTCCCAGCACTTCAAGGTCACAATACCAAAATGAGTGCTTCAGATAC
AAATTCCGCAGTTTATCTCTCTGATACACCAGATCAAGTTAAAGATAAAATTAAGAAACA
TGCTTTCTCTGGTGGTGGTGCTACTAAAGAAGAACAAGAGAAAAATGGTGCTGATCTTTC
AGTTGATATCACTTATGAATATTTAACTTTTATGTTGGAAGATGATGAACACATTAAAAG
ATATCGCTCATAGATATTCGAACTGGTAAAATGATGACTGGTGAAATTACACACAAATTT
TAATTGATCTTATGAATAAAATTATCATTCGTCATAAAGAAGCAAGAGCTAAAATTACTG
ANAAGTTTTATCAACTTTTATGTCAANTCGTAAATTAA
sequence update 2001.11.22
Translated Amino Acid sequence 
---DIIALGFDIQKTFIFSNLEYIHHLYPNVLKIARCVNLNQIQNIFGFKESDAIGKFTF
PPVQAAPCFPDSFPHIFPLNDPEIKNIRCLIPCAIDQDPYFRMTRDIAHRVGHQKPALIH
SKFFPALQGHNTKMSASDTNSAVYLSDTPDQVKDKIKKHAFSGGGATKEEQEKNGADLSV
DITYEYLTFMLEDDEHIKRYRS*ifelvk**lvklhtnfn*sye*nyhss*rsks*ny*x
vlstfmsxrkl
Translated Amino Acid sequence (All Frames) 
Frame A:
---kislhwvlifkkllffqi*nifiifiqmylklqdvli*ikfkifldlknqmqlvnlh
fhqfkqhhvfqihshifsh*miqrlrifvv*fhvqlikihtle*lvilhielviknqhlf
ivnssqhfkvtipk*vlqiqipqfislihqiklkiklrnmlslvvvllkknkrkmvlifq
lislmni*llcwkmmntlkdiahrysnw*nddw*nytqilidlmnkiiirhkearakitx
kfyqllcqxvn*


Frame B:
---ryhcigf*ysknfyffkfriyssslskct*nckmc*fksnskyfwi*rircnw*iyi
stsssstmfsrfiptyfpie*srd*eyslfnsmcn*srsil*nds*yct*swssktstys
**ilpstsrsqyqnecfrykfrslsl*ytrss*r*n*etcflwwwcy*rrtrekwc*sfs
*yhl*ifnfyvgr**th*kislidirtgkmmtgeithkf*lil*iklsfvikkqelkllx
sfinfyvxs*i


Frame C:
---DIIALGFDIQKTFIFSNLEYIHHLYPNVLKIARCVNLNQIQNIFGFKESDAIGKFTF
PPVQAAPCFPDSFPHIFPLNDPEIKNIRCLIPCAIDQDPYFRMTRDIAHRVGHQKPALIH
SKFFPALQGHNTKMSASDTNSAVYLSDTPDQVKDKIKKHAFSGGGATKEEQEKNGADLSV
DITYEYLTFMLEDDEHIKRYRS*ifelvk**lvklhtnfn*sye*nyhss*rsks*ny*x
vlstfmsxrkl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFH545 (VFH545Q) /CSM/VF/VFH5-B/VFH545Q.Seq.d/                       1471   0.0
VFB889 (VFB889Q) /CSM/VF/VFB8-D/VFB889Q.Seq.d/                       1396   0.0
VFI551 (VFI551Q) /CSM/VF/VFI5-C/VFI551Q.Seq.d/                       1390   0.0
VFB371 (VFB371Q) /CSM/VF/VFB3-C/VFB371Q.Seq.d/                       1308   0.0
VSH488 (VSH488Q) /CSM/VS/VSH4-D/VSH488Q.Seq.d/                       1181   0.0
VFM423 (VFM423Q) /CSM/VF/VFM4-A/VFM423Q.Seq.d/                       1124   0.0
VFA133 (VFA133Q) /CSM/VF/VFA1-B/VFA133Q.Seq.d/                       1041   0.0
VFK682 (VFK682Q) /CSM/VF/VFK6-D/VFK682Q.Seq.d/                       1035   0.0
SFH604 (SFH604Q) /CSM/SF/SFH6-A/SFH604Q.Seq.d/                        874   0.0
SSD674 (SSD674Q) /CSM/SS/SSD6-D/SSD674Q.Seq.d/                        737   0.0
own update 2001.12. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CD200153|CD200153.1 MS1-0115T-R321-G04-U.G MS1-0115 Schistosoma mansoni cDNA cloneMS1-0115T-R321-G04.G, mRNA sequence.    80   7e-16  2
CD200156|CD200156.1 MS1-0115T-R321-G08-U.G MS1-0115 Schistosoma mansoni cDNA clone MS1-0115T-R321-G08.G, mRNA sequence.    80   8e-16  2
CD200159|CD200159.1 MS1-0115T-R321-G12-U.G MS1-0115 Schistosoma mansoni cDNA clone MS1-0115T-R321-G12.G, mRNA sequence.    80   8e-16  2
CD200130|CD200130.1 MS1-0115T-R321-D05-U.G MS1-0115 Schistosoma mansoni cDNA clone MS1-0115T-R321-D05.G, mRNA sequence.    80   8e-16  2
CD200161|CD200161.1 MS1-0115T-R321-H04-U.G MS1-0115 Schistosoma mansoni cDNA clone MS1-0115T-R321-H04.G, mRNA sequence.    80   8e-16  2
CD130712|CD130712.1 MG1-0006T-R248-C03-U.G MG1-0006 Schistosoma mansoni cDNA clone MG1-0006T-R248-C03.G, mRNA sequence.    80   8e-16  2
CD200158|CD200158.1 MS1-0115T-R321-G11-U.G MS1-0115 Schistosoma mansoni cDNA clone MS1-0115T-R321-G11.G, mRNA sequence.    80   9e-16  2
CD200154|CD200154.1 MS1-0115T-R321-G05-U.G MS1-0115 Schistosoma mansoni cDNA clone MS1-0115T-R321-G05.G, mRNA sequence.    80   5e-14  2
CD200108|CD200108.1 MS1-0115T-R321-A06-U.G MS1-0115 Schistosoma mansoni cDNA clone MS1-0115T-R321-A06.G, mRNA sequence.    72   2e-13  2
AW756809|AW756809.1 sl27e03.y1 Gm-c1027 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1027-2549 5' similar to SW:SYW_HUMAN P23381 TRYPTOPHANYL-TRNA SYNTHETASE ;, mRNA sequence.    84   2e-13  2
dna update 2004. 3.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC165344_1(BC165344|pid:none) Danio rerio tryptophanyl-tRNA synt...   248   1e-64
AP008218_1042(AP008218|pid:none) Oryza sativa (japonica cultivar...   246   2e-64
BC003450_1(BC003450|pid:none) Mus musculus tryptophanyl-tRNA syn...   244   2e-63
AK004541_1(AK004541|pid:none) Mus musculus adult male lung cDNA,...   244   2e-63
(P32921) RecName: Full=Tryptophanyl-tRNA synthetase, cytoplasmic...   244   2e-63
(Q6P7B0) RecName: Full=Tryptophanyl-tRNA synthetase, cytoplasmic...   244   2e-63
S50053(S50053;S50052;I49391;S31461;S31462) tryptophan-tRNA ligas...   244   3e-63
X69656_1(X69656|pid:none) M.musculus (clone S5) WRS mRNA for try...   244   3e-63
AK150090_1(AK150090|pid:none) Mus musculus bone marrow macrophag...   243   5e-63
AC011437_7(AC011437|pid:none) Arabidopsis thaliana chromosome II...   241   6e-63
protein update 2009. 6.21
PSORT 

 psg: 0.75  gvh: 0.35  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.10  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VFH545 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID VFH545Z
3' end seq. 
>VFH545Z.Seq
NNNNNNNNNNAAAGATATCATTGCATTGGGTTTTGATATTCAAAAAACTTTTATTTTTTC
AAATTTAGAATATATTCATCATCTTTATCCAAATGTACTTAAAATTGCAAGATGTGTTAA
TTTAAATCAAATTCAAAATATTTTTGGATTTAAAGAATCAGATGCAATTGGTAAATTTAC
ATTTCCACCAGTTCAAGCAGCACCATGTTTTCCAGATTCATTCCCACATATTTTCCCATT
GAATGATCCAGAGATTAAGAATATTCGTTGTTTAATTCCATGTGCAATTGATCAAGATCC
ATACTTTAGAATGACTCGTGATATTGCACATAGAGTTGGTCATCAAAAACCAGCACTTAT
TCATAGTAAATTCTTCCCAGCACTTCAAGGTCACAATACCAAAATGAGTGCTTCAGATAC
AAATTCCGCAGTTTATCTCTCTGATACACCAGATCAAGTTAAAGATAAAATTAAGAAACA
TGCTTTCTCTGGTGGTGGTGCTACTAAAGAAGAACAAGAGAAAAATGGTGCTGATCTTTC
AGTTGATATCACTTATGAATATTTAACTTTTATGTTGGAAGATGATGAACACATTAAAAG
ATATCGCTCATAGATATTCGAACTGGTAAAATGATGACTGGTGAAATTACACACAAATTT
TAATTGATCTTATGAATAAAATTATCATTCGTCATAAAGAAGCAAGAGCTAAAATTACTG
ANAAGTTTTATCAACTTTTATGTCAANTCGTAAATTAA
Length of 3' end seq. 758
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -