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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFH475
Library VF
(Link to library)
Clone ID VFH475
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15456-1
Original site URL
Representative seq. ID VFH475P
(Link to Original site)
Representative DNA sequence 
>VFH475 (VFH475Q) /CSM/VF/VFH4-D/VFH475Q.Seq.d/
AATTGTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGC
TACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAGGCTTCAAGAGA
ATCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTAC
CACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTAC
CCCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATC
AAAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGC
CTCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTAT
GTCAGGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAXXXXXXXX
XXGGTGGTGAACTCTCATTCGGTTCAATCGATAACACCAAATACACTGGTGACATTACCT
ACGTCCCATTAACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTATCGATG
GTCAATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTACATCAC
TCATTGCTGGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCT
TAAATGGTGAAGGTGTCTTACTCTGATTGTAGCGTTATCAACACCTTACCAAATGTTACC
ATCACCGTTGCTGGTCGTGAATTTGTTTTAACTNCCAAAAGAATACGTTTTAGAAGTTAC
TGAGTTCGGAAAGNACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGGAA
ATTTNCTGGATNCCTTGGTGATGTTTTCATACTACTGCTTACTATACTGTATTNCGNATT
TTGGTAATAAACAAGTTGGTTTNCGCAACTGCCATGCAAGAGTTAANTTTTTTAAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ivt*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGT
TIPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGA
SSTYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVK---

---GGELSFGSIDNTKYTGDITYVPLTNETYWEFVMDDFAIDGQSAGFCGTTCHAICDSG
TSLIAGPMADITALNEKLGAVILNGEGVLL*l*ryqhltkcyhhrcws*icfnxqkntf*
kllssexteclsgfmgielnmgnxldxlvmfsyycllycixxfgnkqvgxrncharvxff
n
Translated Amino Acid sequence (All Frames) 
Frame A:
nchltyikeneityfnfifsyycfsssfnstiklpsgfkri*kksstkmvkqiicsqcwy
hnpnfrf*rcsilwchyhwyprsslqssfrywfiqlvdsikemsnhcccm*ft*qi*qrc
lkhicrqrn*fhhpir*wcyvrfclsrfrhcwfinc*---

---GGELSFGSIDNTKYTGDITYVPLTNETYWEFVMDDFAIDGQSAGFCGTTCHAICDSG
TSLIAGPMADITALNEKLGAVILNGEGVLL*l*ryqhltkcyhhrcws*icfnxqkntf*
kllssexteclsgfmgielnmgnxldxlvmfsyycllycixxfgnkqvgxrncharvxff
n

Frame B:
ivt*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGT
TIPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGA
SSTYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVK---

---vvnshsvqsitpntlvtlptsh*ptkpignslwmtllsmvnqlvsvvllvtqfaiqv
hhsllvqwlillpsmkn*vlss*mvkvsysdcsvintlpnvtitvagrefvltxkrirfr
sy*vrkxlnv*vdlwvss*tweixwxpw*cfhttayytvxrilvinklvxatamqelxfl

Frame C:
lspdlhkrk*nylf*lyf*lllf*lkl*qyh*tsirlqenleeefhkngqtdyllsmlvp
qsqfqilkmlntmvplplvpqvkpsk*fsilvhptcgfhqrnvqslllhviyitnitavp
qahmsptelispsntvvvlcqvlslkipsllvh*ll---

---w*tlirfnr*hqihw*hylrpinqrnllgiryg*lcyrwsiswflwyylsrnlrfry
ithcwsng*yycpq*kircchlkw*rcltlivalstpyqmlpspllvvnlf*lpkeyvle
vtefgkx*mfewiygyrvkhgkfxgxlgdvfillltilyxxfw**tswfxqlpcks*xf*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFH475 (VFH475Q) /CSM/VF/VFH4-D/VFH475Q.Seq.d/                       1907   0.0
VFE111 (VFE111Q) /CSM/VF/VFE1-A/VFE111Q.Seq.d/                        936   0.0
VFO736 (VFO736Q) /CSM/VF/VFO7-B/VFO736Q.Seq.d/                        928   0.0
VFO719 (VFO719Q) /CSM/VF/VFO7-A/VFO719Q.Seq.d/                        928   0.0
VFO689 (VFO689Q) /CSM/VF/VFO6-D/VFO689Q.Seq.d/                        928   0.0
VFO526 (VFO526Q) /CSM/VF/VFO5-B/VFO526Q.Seq.d/                        928   0.0
VFO331 (VFO331Q) /CSM/VF/VFO3-B/VFO331Q.Seq.d/                        928   0.0
VFN875 (VFN875Q) /CSM/VF/VFN8-D/VFN875Q.Seq.d/                        928   0.0
VFN873 (VFN873Q) /CSM/VF/VFN8-D/VFN873Q.Seq.d/                        928   0.0
VFN780 (VFN780Q) /CSM/VF/VFN7-D/VFN780Q.Seq.d/                        928   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

Y16962|Y16962.1 Dictyostelium discoideum mRNA for cathepsin D.    914   0.0    9
AJ243946|AJ243946.1 Dictyostelium discoideum ctsD gene for cathepsin D, exons 1 to 2.   755   0.0    10
AX059531|AX059531.1 Sequence 264 from Patent WO0055325.            62   2e-05  1
AL161500|AL161500.2 Arabidopsis thaliana DNA chromosome 4, contig fragment No. 12.    62   2e-05  1
BE525959|BE525959.1 M64C07STM Arabidopsis developing seed Arabidopsis thaliana cDNA clone 600034526R1 5', mRNA sequence.    62   2e-05  1
AL762930|AL762930.1 Arabidopsis thaliana T-DNA flanking sequence GK-030C11-011522.    62   2e-05  1
CD825453|CD825453.1 BN25.060N03F011129 BN25 Brassica napus cDNA clone BN25060N03, mRNA sequence.    62   2e-05  1
AF076243|AF076243.1 Arabidopsis thaliana BAC T26N6 from chromosome IV at 19.3 cM, complete sequence.    62   2e-05  1
AV567523|AV567523.1 Arabidopsis thaliana cDNA clone:SQL15g03F, 3' end.    62   2e-05  1
CB264640|CB264640.1 48-E014661-035-002-P12-T7R MPIZ-ADIS-035 Arabidopsis thaliana cDNA clone MPIZp2000P122Q 5-PRIME, mRNA sequence.    62   2e-05  1
dna update 2004. 1.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ010007_1(DQ010007|pid:none) Bombyx mori CathD mRNA, complete c...   144   5e-33
AB106552_1(AB106552|pid:none) Todarodes pacificus tpaD mRNA for ...   144   6e-33
AF454831_1(AF454831|pid:none) Apriona germari cathepsin D mRNA, ...   141   4e-32
EF070454_1(EF070454|pid:none) Maconellicoccus hirsutus clone WHM...   139   1e-31
EF213114_1(EF213114|pid:none) Penaeus monodon cathepsin D mRNA, ...   139   1e-31
FJ654712_1(FJ654712|pid:none) Chrysomela tremulae aspartic prote...   138   3e-31
AJ417035_1(AJ417035|pid:none) Pleurotus ostreatus partial mRNA f...   137   6e-31
(Q03168) RecName: Full=Lysosomal aspartic protease;          EC=...   136   1e-30
CP001577_36(CP001577|pid:none) Micromonas sp. RCC299 chromosome ...   134   7e-30
(O93428) RecName: Full=Cathepsin D;          EC=3.4.23.5; Flags:...   134   7e-30
protein update 2009. 6.20
PSORT 

 psg: 0.98  gvh: 0.77  alm: 0.46  top: 0.57  tms: 0.00  mit: 0.36  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 20.0 %: mitochondrial
	 12.0 %: cytoplasmic
	  8.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: nuclear
	  4.0 %: endoplasmic reticulum

>> prediction for VFH475 is exc
5' end seq. ID VFH475F
5' end seq. 
>VFH475F.Seq
AATTGTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGC
TACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAGGCTTCAAGAGA
ATCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTAC
CACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTAC
CCCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATC
AAAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGC
CTCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTAT
GTCAGGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAA--------
--
Length of 5' end seq. 472
3' end seq. ID VFH475Z
3' end seq. 
>VFH475Z.Seq
----------GGTGGTGAACTCTCATTCGGTTCAATCGATAACACCAAATACACTGGTGA
CATTACCTACGTCCCATTAACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGC
TATCGATGGTCAATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGG
TACATCACTCATTGCTGGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGC
TGTCATCTTAAATGGTGAAGGTGTCTTACTCTGATTGTAGCGTTATCAACACCTTACCAA
ATGTTACCATCACCGTTGCTGGTCGTGAATTTGTTTTAACTNCCAAAAGAATACGTTTTA
GAAGTTACTGAGTTCGGAAAGNACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTAAA
CATGGGAAATTTNCTGGATNCCTTGGTGATGTTTTCATACTACTGCTTACTATACTGTAT
TNCGNATTTTGGTAATAAACAAGTTGGTTTNCGCAACTGCCATGCAAGAGTTAANTTTTT
TAAT
Length of 3' end seq. 534
Connected seq. ID VFH475P
Connected seq. 
>VFH475P.Seq
AATTGTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGC
TACTATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAGGCTTCAAGAGA
ATCTAGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTAC
CACAATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTAC
CCCAGGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATC
AAAGAAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGC
CTCAAGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTAT
GTCAGGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAA--------
--GGTGGTGAACTCTCATTCGGTTCAATCGATAACACCAAATACACTGGTGACATTACCT
ACGTCCCATTAACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTATCGATG
GTCAATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTACATCAC
TCATTGCTGGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCT
TAAATGGTGAAGGTGTCTTACTCTGATTGTAGCGTTATCAACACCTTACCAAATGTTACC
ATCACCGTTGCTGGTCGTGAATTTGTTTTAACTNCCAAAAGAATACGTTTTAGAAGTTAC
TGAGTTCGGAAAGNACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGGAA
ATTTNCTGGATNCCTTGGTGATGTTTTCATACTACTGCTTACTATACTGTATTNCGNATT
TTGGTAATAAACAAGTTGGTTTNCGCAACTGCCATGCAAGAGTTAANTTTTTTAAT
Length of connected seq. 1006
Full length Seq ID -
Full length Seq. -
Length of full length seq. -