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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFH473
Library VF
(Link to library)
Clone ID VFH473
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13945-1
Original site URL
Representative seq. ID VFH473P
(Link to Original site)
Representative DNA sequence 
>VFH473 (VFH473Q) /CSM/VF/VFH4-D/VFH473Q.Seq.d/
ATTTTAAGATTTGTTCAGCAACAGGATCATCAGAATCATCATCAATTTCAAATTCATTTG
ATAATGAATATAGACAAGAATCATTTAATAAACCAACAATGAAAGTTAATAAGAAACCAC
AATATAAAGATTTATCAAATCAAGAACCATTGGTAAAAAAATTTAAAGGTAATGATAGTA
TTGGTTATATTTTACAAATTTCTGATGTACATTTCGATCCAGATTATAAAGTTGGTTCAA
ATCCAAATTGTGGTAGACCATTATGTTGTAGAGATGGTGTAGGTTCAGCAGGTCCAATAG
GTCATTACCTTTGCGATATCCCATTTAGTACTGTTGAATTGATTTTCCAACATTTGGCAA
CATTGACTGATCAATTGGATTTCATAGTTTGGACAGGTGAAAATCCACCACACAATGTAT
GGGAACAATCACAAGCTCAACAAGAGTTGGCCACCGCAACATTGGCACAGGTCATTCAAA
AGACTTTCCCAAATACACCAGTTTTACCATCGTTGGGTAATCATGAAGCTTXXXXXXXXX
XCAATACATTGGAACAAGCTCAATCCAATGGTGAAAAGGTTTTAATCATTGGTCATATTC
CATGCACTGTTAAAAGTGCTTCAACCGATGGTTGGTGTGCAATGTATGAACAAGTGGTAG
GTCAATTCTCTGATGTTATCATTGGTCAATTGTATGGCCATACTCATTACGATCAATTTT
CAGTATTCTCCGATGTTGCAACTCATACAATTCCAACCGGTATGAATTATATCGTTCCAT
CATTAACAACCTATCAAAATCATGAACCTGGTTATCGTATTTATCAATTTGATTATTCAA
CTAATCAAATTGTAAACTACTATCAATATCATGCAAACATTACTGAAGCAAATGAAACTG
GTGCCTTAAATTTCCAATTGACTTATAGTGCTAAAGAACTCTATAATATGGATGATCTTT
CACCTACCTCTTGGACAAAAGTTGCCAATCAAATGAAAACTAATTCAACAATGTTTAATA
GTTATTTCGAAAATNCTTAGTAGTTCACCAATTAAAGA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
FKICSATGSSESSSISNSFDNEYRQESFNKPTMKVNKKPQYKDLSNQEPLVKKFKGNDSI
GYILQISDVHFDPDYKVGSNPNCGRPLCCRDGVGSAGPIGHYLCDIPFSTVELIFQHLAT
LTDQLDFIVWTGENPPHNVWEQSQAQQELATATLAQVIQKTFPNTPVLPSLGNHEA---

---NTLEQAQSNGEKVLIIGHIPCTVKSASTDGWCAMYEQVVGQFSDVIIGQLYGHTHYD
QFSVFSDVATHTIPTGMNYIVPSLTTYQNHEPGYRIYQFDYSTNQIVNYYQYHANITEAN
ETGALNFQLTYSAKELYNMDDLSPTSWTKVANQMKTNSTMFNSYFENX**ftn*r
Translated Amino Acid sequence (All Frames) 
Frame A:
ilrfvqqqdhqnhhqfqihlimnidknhlinqq*klirnhnikiyqiknhw*knlkvmiv
lvifykflmyisiqiiklvqiqivvdhyvvemv*vqqvq*vitfaishlvlln*fsniwq
h*linwis*fgqvkihhtmygnnhklnkswppqhwhrsfkrlsqihqfyhrwvimkl---

---qyigtssiqw*kgfnhwsysmhc*kcfnrwlvcnv*tsgrsil*cyhwsivwpyslr
sifsilrccnsynsnryelyrsiinnlsks*twlsylsi*lfn*snckllsisckhy*sk
*nwclkfpidl*c*rtl*yg*sftylldkscqsnen*fnnv**lfrkxlvvhqlk

Frame B:
f*dlfsnriiriiinfkfi***i*trii**tnnes**etti*rfiksrtigkki*r***y
wlyftnf*ctfrsrl*swfksklw*timl*rwcrfsrsnrslplrypi*yc*idfptfgn
id*sigfhsldr*ksttqcmgtitsstrvghrnigtghskdfpkytsftivg*s*s---

---NTLEQAQSNGEKVLIIGHIPCTVKSASTDGWCAMYEQVVGQFSDVIIGQLYGHTHYD
QFSVFSDVATHTIPTGMNYIVPSLTTYQNHEPGYRIYQFDYSTNQIVNYYQYHANITEAN
ETGALNFQLTYSAKELYNMDDLSPTSWTKVANQMKTNSTMFNSYFENX**ftn*r

Frame C:
FKICSATGSSESSSISNSFDNEYRQESFNKPTMKVNKKPQYKDLSNQEPLVKKFKGNDSI
GYILQISDVHFDPDYKVGSNPNCGRPLCCRDGVGSAGPIGHYLCDIPFSTVELIFQHLAT
LTDQLDFIVWTGENPPHNVWEQSQAQQELATATLAQVIQKTFPNTPVLPSLGNHEA---

---ihwnklnpmvkrf*slvifhallkvlqpmvgvqcmnkw*vnslmlslvncmailiti
nfqyspmlqliqfqpv*iisfhh*qpikimnlvivfinliiqlikl*ttinimqtllkqm
klvp*isn*livlknsiiwmifhlplgqklpik*kliqqcliviskxlssspik
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFH473 (VFH473Q) /CSM/VF/VFH4-D/VFH473Q.Seq.d/                       1990   0.0
VHG223 (VHG223Q) /CSM/VH/VHG2-A/VHG223Q.Seq.d/                       1009   0.0
VHC519 (VHC519Q) /CSM/VH/VHC5-A/VHC519Q.Seq.d/                       1009   0.0
VHB601 (VHB601Q) /CSM/VH/VHB6-A/VHB601Q.Seq.d/                       1009   0.0
VFL234 (VFL234Q) /CSM/VF/VFL2-B/VFL234Q.Seq.d/                       1001   0.0
VHJ154 (VHJ154Q) /CSM/VH/VHJ1-C/VHJ154Q.Seq.d/                        997   0.0
VFH433 (VFH433Q) /CSM/VF/VFH4-B/VFH433Q.Seq.d/                        787   0.0
SLK658 (SLK658Q) /CSM/SL/SLK6-C/SLK658Q.Seq.d/                        678   0.0
VHG422 (VHG422Q) /CSM/VH/VHG4-A/VHG422Q.Seq.d/                        329   5e-89
AFI211 (AFI211Q) /CSM/AF/AFI2-A/AFI211Q.Seq.d/                        163   7e-39
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    38   0.012  16
AC115592|AC115592.2 Dictyostelium discoideum chromosome 2 map 1-125959 strain AX4, complete sequence.    34   0.019  9
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    32   0.023  12
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    34   0.027  17
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    44   0.073  11
CG755729|CG755729.1 P051-1-H10.yc Ppa EcoRI BAC Library Pristionchus pacificus genomic, genomic survey sequence.    48   0.25   1
CG755728|CG755728.1 P051-1-H10.yb Ppa EcoRI BAC Library Pristionchus pacificus genomic, genomic survey sequence.    48   0.25   1
AL445563|AL445563.1 Mycoplasma pulmonis (strain UAB CTIP) complete genome; segment 1/3.    36   0.41   13
AP004170|AP004170.1 Mycoplasma penetrans genomic DNA, complete sequence, section 1/5.    36   0.53   13
AP003431|AP003431.2 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 1, BAC clone:B1099D03.    40   0.62   6
dna update 2004. 1.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q0VD19) RecName: Full=Sphingomyelin phosphodiesterase;         ...   116   1e-24
A39825(S27009;S36357;A39825;JX0276;S06957;I55567;A42801;) sphing...   114   7e-24
X63600_1(X63600|pid:none) H.sapiens genes for acid sphingomyelin...   114   7e-24
AK292388_1(AK292388|pid:none) Homo sapiens cDNA FLJ77600 complet...   114   7e-24
X59960_1(X59960|pid:none) H.sapiens mRNA for sphingomyelinase.        113   1e-23
(P17405) RecName: Full=Sphingomyelin phosphodiesterase;         ...   113   1e-23
M81780_2(M81780|pid:none) Homo sapiens acid sphingomyelinase (SM...   113   1e-23
(Q04519) RecName: Full=Sphingomyelin phosphodiesterase;         ...   113   1e-23
AK149579_1(AK149579|pid:none) Mus musculus adult male liver tumo...   113   1e-23
BC083780_1(BC083780|pid:none) Rattus norvegicus sphingomyelin ph...   112   3e-23
protein update 2009. 6.20
PSORT 

 psg: 0.81  gvh: 0.45  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.03  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 20.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VFH473 is cyt
5' end seq. ID VFH473F
5' end seq. 
>VFH473F.Seq
ATTTTAAGATTTGTTCAGCAACAGGATCATCAGAATCATCATCAATTTCAAATTCATTTG
ATAATGAATATAGACAAGAATCATTTAATAAACCAACAATGAAAGTTAATAAGAAACCAC
AATATAAAGATTTATCAAATCAAGAACCATTGGTAAAAAAATTTAAAGGTAATGATAGTA
TTGGTTATATTTTACAAATTTCTGATGTACATTTCGATCCAGATTATAAAGTTGGTTCAA
ATCCAAATTGTGGTAGACCATTATGTTGTAGAGATGGTGTAGGTTCAGCAGGTCCAATAG
GTCATTACCTTTGCGATATCCCATTTAGTACTGTTGAATTGATTTTCCAACATTTGGCAA
CATTGACTGATCAATTGGATTTCATAGTTTGGACAGGTGAAAATCCACCACACAATGTAT
GGGAACAATCACAAGCTCAACAAGAGTTGGCCACCGCAACATTGGCACAGGTCATTCAAA
AGACTTTCCCAAATACACCAGTTTTACCATCGTTGGGTAATCATGAAGCTT---------
-
Length of 5' end seq. 531
3' end seq. ID VFH473Z
3' end seq. 
>VFH473Z.Seq
----------CAATACATTGGAACAAGCTCAATCCAATGGTGAAAAGGTTTTAATCATTG
GTCATATTCCATGCACTGTTAAAAGTGCTTCAACCGATGGTTGGTGTGCAATGTATGAAC
AAGTGGTAGGTCAATTCTCTGATGTTATCATTGGTCAATTGTATGGCCATACTCATTACG
ATCAATTTTCAGTATTCTCCGATGTTGCAACTCATACAATTCCAACCGGTATGAATTATA
TCGTTCCATCATTAACAACCTATCAAAATCATGAACCTGGTTATCGTATTTATCAATTTG
ATTATTCAACTAATCAAATTGTAAACTACTATCAATATCATGCAAACATTACTGAAGCAA
ATGAAACTGGTGCCTTAAATTTCCAATTGACTTATAGTGCTAAAGAACTCTATAATATGG
ATGATCTTTCACCTACCTCTTGGACAAAAGTTGCCAATCAAATGAAAACTAATTCAACAA
TGTTTAATAGTTATTTCGAAAATNCTTAGTAGTTCACCAATTAAAGA
Length of 3' end seq. 517
Connected seq. ID VFH473P
Connected seq. 
>VFH473P.Seq
ATTTTAAGATTTGTTCAGCAACAGGATCATCAGAATCATCATCAATTTCAAATTCATTTG
ATAATGAATATAGACAAGAATCATTTAATAAACCAACAATGAAAGTTAATAAGAAACCAC
AATATAAAGATTTATCAAATCAAGAACCATTGGTAAAAAAATTTAAAGGTAATGATAGTA
TTGGTTATATTTTACAAATTTCTGATGTACATTTCGATCCAGATTATAAAGTTGGTTCAA
ATCCAAATTGTGGTAGACCATTATGTTGTAGAGATGGTGTAGGTTCAGCAGGTCCAATAG
GTCATTACCTTTGCGATATCCCATTTAGTACTGTTGAATTGATTTTCCAACATTTGGCAA
CATTGACTGATCAATTGGATTTCATAGTTTGGACAGGTGAAAATCCACCACACAATGTAT
GGGAACAATCACAAGCTCAACAAGAGTTGGCCACCGCAACATTGGCACAGGTCATTCAAA
AGACTTTCCCAAATACACCAGTTTTACCATCGTTGGGTAATCATGAAGCTT---------
-CAATACATTGGAACAAGCTCAATCCAATGGTGAAAAGGTTTTAATCATTGGTCATATTC
CATGCACTGTTAAAAGTGCTTCAACCGATGGTTGGTGTGCAATGTATGAACAAGTGGTAG
GTCAATTCTCTGATGTTATCATTGGTCAATTGTATGGCCATACTCATTACGATCAATTTT
CAGTATTCTCCGATGTTGCAACTCATACAATTCCAACCGGTATGAATTATATCGTTCCAT
CATTAACAACCTATCAAAATCATGAACCTGGTTATCGTATTTATCAATTTGATTATTCAA
CTAATCAAATTGTAAACTACTATCAATATCATGCAAACATTACTGAAGCAAATGAAACTG
GTGCCTTAAATTTCCAATTGACTTATAGTGCTAAAGAACTCTATAATATGGATGATCTTT
CACCTACCTCTTGGACAAAAGTTGCCAATCAAATGAAAACTAATTCAACAATGTTTAATA
GTTATTTCGAAAATNCTTAGTAGTTCACCAATTAAAGA
Length of connected seq. 1048
Full length Seq ID -
Full length Seq. -
Length of full length seq. -