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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFH460
Library VF
(Link to library)
Clone ID VFH460
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID VFH460P
(Link to Original site)
Representative DNA sequence 
>VFH460 (VFH460Q) /CSM/VF/VFH4-C/VFH460Q.Seq.d/
AACTTTAAAGAAAGAATAAACAACCATGGGTAAAGAGAAAACTCACATTAACATTGTCGT
CATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTACAAATGTGG
TGGTATCGATAAGAGAGTTATCGAAAAGTATGAAAAGGAAGCTTCTGAAATGGGTAAACA
ATCCTTCAAATACGCCTGGGTTATGGATAAATTAAAGGCTGAACGTGAAAGAGGTATCAC
CATCGATATTGCTTTATGGAAATTCGAAACCTCCAAATACTACTTCACCATCATTGATGC
CCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCCCAAGCCGATTGTGC
CGTCTTAGTCATTGCCTCCCCAACTGGTGAATTCGAAGCTGGTATCGCCAAAAACGGTCA
AACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAATGATCGTTGCTAT
CAACAAGATGGATGAAAAATCAACCAACTACTCACAAGCCCGTTACGACGAAATTGTCAA
GGAAGTTTCATCTTTCATCAAAAAGATTGGTTACAATCCAXXXXXXXXXXTTAGATTGTC
ACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTG
GTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTA
TGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATACCCACCAT
TAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCATCAAATCAA
CCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAA
AGAAATAAATAAATAAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
tlkke*TTMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYEKEASEMGKQ
SFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMITGTSQADCA
VLVIASPTGEFEAGIAKNGQTREHALLAYTLGVKQMIVAINKMDEKSTNYSQARYDEIVK
EVSSFIKKIGYNP---

---LDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMCVES
FTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*inn
Translated Amino Acid sequence (All Frames) 
Frame A:
nfkerinnhg*rensh*hcrhwsrrcw*inhhwsfnlqmwwyr*esyrkv*kgsf*ng*t
ilqirlgyg*ikg*t*kryhhrycfmeirnlqillhhh*cprsqrfhqeydywylpsrlc
rlshclpnw*irswyrqkrsns*trspclhfrcqtndrcyqqdg*kinqlltsplrrncq
gsfifhqkdwlqs---

---LDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMCVES
FTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*inn

Frame B:
tlkke*TTMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYEKEASEMGKQ
SFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMITGTSQADCA
VLVIASPTGEFEAGIAKNGQTREHALLAYTLGVKQMIVAINKMDEKSTNYSQARYDEIVK
EVSSFIKKIGYNP---

---*ivtlltlpvnslkssiklivvlvpslpkkvlppss*rmvmplwsn*phlvqcvlnp
slnthh*vvspsei*dkpspsvssnqpsrkpqvkpvirkvllphqkrnk*i

Frame C:
l*rknkqpwvkrkltltlsslvt*mlvnqpplvi*stnvvvsirelsksmkrkllkwvnn
psntpglwin*rlnvkevspsillygnskppnttspslmpqvteissri*llvppkpivp
s*slppqlvnsklvspktvklvntlslltl*vsnk*sllstrwmknqptthkpvttklsr
kfhlsskrlvti---

---rlshcshcl*ih*nrr*s*ssywcrrcqrrycrrrlkew*crygrinpissnvc*il
h*iptirsfrrqryetnrrrrchqinrqespr*sr**ercccpikkeink*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHO616 (VHO616Q) /CSM/VH/VHO6-A/VHO616Q.Seq.d/                       1150   0.0
VFO278 (VFO278Q) /CSM/VF/VFO2-D/VFO278Q.Seq.d/                       1150   0.0
VFN814 (VFN814Q) /CSM/VF/VFN8-A/VFN814Q.Seq.d/                       1150   0.0
VFN579 (VFN579Q) /CSM/VF/VFN5-D/VFN579Q.Seq.d/                       1150   0.0
VFN340 (VFN340Q) /CSM/VF/VFN3-B/VFN340Q.Seq.d/                       1150   0.0
VFN256 (VFN256Q) /CSM/VF/VFN2-C/VFN256Q.Seq.d/                       1150   0.0
VFN184 (VFN184Q) /CSM/VF/VFN1-D/VFN184Q.Seq.d/                       1150   0.0
VFN139 (VFN139Q) /CSM/VF/VFN1-B/VFN139Q.Seq.d/                       1150   0.0
VFM716 (VFM716Q) /CSM/VF/VFM7-A/VFM716Q.Seq.d/                       1150   0.0
VFM630 (VFM630Q) /CSM/VF/VFM6-B/VFM630Q.Seq.d/                       1150   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X55973|X55973.1 D. discoideum EF1-I gene for elongation factor 1 alpha.  1082   0.0    1
X55972|X55972.1 D. discoideum EF1-II gene for elongation factor 1 alpha.   886   0.0    2
AF016242|AF016242.1 Dictyostelium discoideum protein synthesis elongation factor 1-alpha (tef2) gene, partial cds.   682   0.0    3
M29934|M29934.1 C.albicans elongation factor 1-alpha (TEF-1) gene, complete cds.   192   e-121  6
M29935|M29935.1 C.albicans elongation factor 1-alpha (TEF-2) gene, complete cds.   264   e-117  5
AF003560|AF003560.1 Austrosimulium bancrofti elongation factor-1 alpha (EF1-a) gene, partial cds.   190   e-100  5
AF056109|AF056109.1 Telotrochidium henneguyii translation elongation factor 1-alpha (TEF1) gene, partial cds.   137   1e-98  8
AF157274|AF157274.1 Phascolomyces articulosus translation elongation factor 1-alpha (EF-1alpha) gene, partial cds.   105   1e-98  9
CF433932|CF433932.1 NIT1_31_E10.b1_A002 Nitrogen-deficient seedlings Sorghum bicolor cDNA clone NIT1_31_E10_A002 3', mRNA sequence.   270   2e-98  2
AX831110|AX831110.1 Sequence 1830 from Patent WO03072602.         103   8e-98  8
dna update 2004. 1.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   361   2e-98
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   361   2e-98
(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   361   2e-98
BC092884_1(BC092884|pid:none) Danio rerio zgc:110335, mRNA (cDNA...   322   8e-87
BC060907_1(BC060907|pid:none) Danio rerio zgc:73138, mRNA (cDNA ...   320   3e-86
BC075885_1(BC075885|pid:none) Danio rerio zgc:92085, mRNA (cDNA ...   320   5e-86
(P13549) RecName: Full=Elongation factor 1-alpha, somatic form; ...   320   5e-86
(Q90835) RecName: Full=Elongation factor 1-alpha 1;          Sho...   319   7e-86
AK167843_1(AK167843|pid:none) Mus musculus TIB-55 BB88 cDNA, RIK...   319   7e-86
AB174733_1(AB174733|pid:none) Macaca fascicularis brain cDNA clo...   319   7e-86
protein update 2009. 6.20
PSORT 

 psg: 0.84  gvh: 0.34  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VFH460 is cyt
5' end seq. ID VFH460F
5' end seq. 
>VFH460F.Seq
AACTTTAAAGAAAGAATAAACAACCATGGGTAAAGAGAAAACTCACATTAACATTGTCGT
CATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTACAAATGTGG
TGGTATCGATAAGAGAGTTATCGAAAAGTATGAAAAGGAAGCTTCTGAAATGGGTAAACA
ATCCTTCAAATACGCCTGGGTTATGGATAAATTAAAGGCTGAACGTGAAAGAGGTATCAC
CATCGATATTGCTTTATGGAAATTCGAAACCTCCAAATACTACTTCACCATCATTGATGC
CCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCCCAAGCCGATTGTGC
CGTCTTAGTCATTGCCTCCCCAACTGGTGAATTCGAAGCTGGTATCGCCAAAAACGGTCA
AACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAATGATCGTTGCTAT
CAACAAGATGGATGAAAAATCAACCAACTACTCACAAGCCCGTTACGACGAAATTGTCAA
GGAAGTTTCATCTTTCATCAAAAAGATTGGTTACAATCCA----------
Length of 5' end seq. 580
3' end seq. ID VFH460Z
3' end seq. 
>VFH460Z.Seq
----------TTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCGA
TAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTT
AAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATC
CTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCGC
CGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAAAGG
TGCTGCTGCCCCATCAAAAAAGAAATAAATAAATAAT
Length of 3' end seq. 327
Connected seq. ID VFH460P
Connected seq. 
>VFH460P.Seq
AACTTTAAAGAAAGAATAAACAACCATGGGTAAAGAGAAAACTCACATTAACATTGTCGT
CATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTACAAATGTGG
TGGTATCGATAAGAGAGTTATCGAAAAGTATGAAAAGGAAGCTTCTGAAATGGGTAAACA
ATCCTTCAAATACGCCTGGGTTATGGATAAATTAAAGGCTGAACGTGAAAGAGGTATCAC
CATCGATATTGCTTTATGGAAATTCGAAACCTCCAAATACTACTTCACCATCATTGATGC
CCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCCCAAGCCGATTGTGC
CGTCTTAGTCATTGCCTCCCCAACTGGTGAATTCGAAGCTGGTATCGCCAAAAACGGTCA
AACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAATGATCGTTGCTAT
CAACAAGATGGATGAAAAATCAACCAACTACTCACAAGCCCGTTACGACGAAATTGTCAA
GGAAGTTTCATCTTTCATCAAAAAGATTGGTTACAATCCA----------TTAGATTGTC
ACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTG
GTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTA
TGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATACCCACCAT
TAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCATCAAATCAA
CCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAA
AGAAATAAATAAATAAT
Length of connected seq. 907
Full length Seq ID -
Full length Seq. -
Length of full length seq. -