>VFH459 (VFH459Q) /CSM/VF/VFH4-C/VFH459Q.Seq.d/
AAAAATNATACACACACCAAAATATAAAATATATACTTAAAAAGATGATTTCAAACCTTT
CAAAAGTTTTAAACTCAAATGTAAAGAGAATGTATACTACAGCAAAAAACCTTGAATCAG
TTGTAATTGTTTCAGCAGTTAGAACACCAATTGGTTCAATTGGTGGTTCATTATCAACAA
TACCAGGTACAAAATTATCATCAATAACAATTGAAGAAGCAGTAAAGAGAGCAGGTATTA
AACCATCAGATGTTGATGAAGCAATCATTGGTAATGTAATTTCAGCCAATTTAGGACAAG
CACCAGCACGTCAATGTGCATTGGGAGCTGGTTTAGAACAAAAAACCATCACAACCACCA
TCAATAAAGTATGTTCATCAGGTATGAAAGCAATTGTATTTGGCGCACAATCAATCGCAT
TGGGTCACAGCAAAACTGTGGTTGCAGGTGGTTTCGAATCAATGTCACAAGTGCCATACT
ATGCCGATAAAATGAGATTCGGTGCAAAGTATGGCAACCAAACATTCATCGATGGTTTGG
TTAGAGATGGTTTAGCCGACGCATACAACGGAAGTGCTATGGGTGTTTGTGGAGATGATT
GTGCCGATAAATATAAGATCACTCGTGXXXXXXXXXXCTTTGAAATCAATGAAGCCTTTG
CTGTTGTTGGTCTTGCAAATGCAAAGATCTTAGATATCGATCATAATAAACTCAATGTTA
ATGGTGGTGCCGTTGCATTAGGTCATCCAATTGGTTCATCAGGTTGTCGTATTGTTGTTA
CTCTTACTCATTTACTTCAAAATAAAAATCTTAAATATGGTGTTGCTGCTATTTGTAATG
GTGGTGGAGGTAGTACTGCTTTAGTTTTAGAAAAATTATAAATA

KXYTHQNIKYILKKMISNLSKVLNSNVKRMYTTAKNLESVVIVSAVRTPIGSIGGSLSTI
PGTKLSSITIEEAVKRAGIKPSDVDEAIIGNVISANLGQAPARQCALGAGLEQKTITTTI
NKVCSSGMKAIVFGAQSIALGHSKTVVAGGFESMSQVPYYADKMRFGAKYGNQTFIDGLV
RDGLADAYNGSAMGVCGDDCADKYKITR---

---FEINEAFAVVGLANAKILDIDHNKLNVNGGAVALGHPIGSSGCRIVVTLTHLLQNKN
LKYGVAAICNGGGGSTALVLEKL*i

Frame A:
knxthtki*niylkr*fqtfqkf*tqm*recilqqktlnql*lfqqlehqlvqlvvhyqq
yqvqnyhq*qlkkq*reqvlnhqmlmkqslvm*fqpi*dkhqhvnvhwelv*nkkpsqpp
sikyvhqv*kqlylahnqshwvtaklwlqvvsnqchkchtmpik*dsvqsmatkhssmvw
lemv*pthttevlwvfvemivpinirslv---

---l*nq*slcccwsckckdlryrs**tqc*wwcrcirssnwfirlsyccysysftsk*k
s*iwcccyl*wwwr*ycfsfrkiin

Frame B:
kxihtpkykiyt*kddfkpfksfklkckenvyyskkp*iscncfss*ntnwfnwwfiinn
trykiiinnn*rsskesry*tirc**snhw*cnfsqfrtststsmcigswfrtknhhnhh
q*smfiryesnciwrtinrigsqqncgcrwfrinvtsailcr*neirckvwqpnihrwfg
*rwfsrriqrkcygclwr*lcr*i*dhs---

---FEINEAFAVVGLANAKILDIDHNKLNVNGGAVALGHPIGSSGCRIVVTLTHLLQNKN
LKYGVAAICNGGGGSTALVLEKL*i

Frame C:
KXYTHQNIKYILKKMISNLSKVLNSNVKRMYTTAKNLESVVIVSAVRTPIGSIGGSLSTI
PGTKLSSITIEEAVKRAGIKPSDVDEAIIGNVISANLGQAPARQCALGAGLEQKTITTTI
NKVCSSGMKAIVFGAQSIALGHSKTVVAGGFESMSQVPYYADKMRFGAKYGNQTFIDGLV
RDGLADAYNGSAMGVCGDDCADKYKITR---

---lksmkpllllvlqmqrs*isiiinsmlmvvplh*viqlvhqvvvlllllliyfkiki
lnmvlllfvmvvevvll*f*knyk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFH459 (VFH459Q) /CSM/VF/VFH4-C/VFH459Q.Seq.d/                       1687   0.0
AFG237 (AFG237Q) /CSM/AF/AFG2-B/AFG237Q.Seq.d/                       1237   0.0
VFA210 (VFA210Q) /CSM/VF/VFA2-A/VFA210Q.Seq.d/                       1211   0.0
CHA249 (CHA249Q) /CSM/CH/CHA2-C/CHA249Q.Seq.d/                       1203   0.0
SFF687 (SFF687Q) /CSM/SF/SFF6-D/SFF687Q.Seq.d/                       1199   0.0
VFI126 (VFI126Q) /CSM/VF/VFI1-B/VFI126Q.Seq.d/                       1193   0.0
VFM184 (VFM184Q) /CSM/VF/VFM1-D/VFM184Q.Seq.d/                       1189   0.0
VFA288 (VFA288Q) /CSM/VF/VFA2-D/VFA288Q.Seq.d/                       1187   0.0
VFM844 (VFM844Q) /CSM/VF/VFM8-B/VFM844Q.Seq.d/                       1183   0.0
SLH886 (SLH886Q) /CSM/SL/SLH8-D/SLH886Q.Seq.d/                       1181   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.  1084   0.0    7
BQ403038|BQ403038.1 GA__Ed0053B10f Gossypium arboreum 7-10 dpa fiber library Gossypium arboreum cDNA clone GA__Ed0053B10f, mRNA sequence.    62   6e-08  2
CD486570|CD486570.1 CRH4.4H04 Cotton Root and Hypocotyl Lambda ZIPLOX Library (CRH) Gossypium hirsutum cDNA clone CRH4.4H04 5' similar to acetyl Co-A acetyltransferase, mRNA sequence.    62   7e-08  2
AR320717|AR320717.1 Sequence 3267 from patent US 6562958.          52   6e-06  2
CB971300|CB971300.1 CAB10005_IIIa_Fa_H08 Cabernet Sauvignon Flower Pre-bloom - CAB1 Vitis vinifera cDNA clone CAB10005_IIIa_Fa_H08 5', mRNA sequence.    48   6e-06  4
CB975981|CB975981.1 CAB40001_IIa_Fa_F09 Cabernet Sauvignon Berry - CAB4 Vitis vinifera cDNA clone CAB40001_IIa_Fa_F09 5', mRNA sequence.    48   1e-05  4
CF511327|CF511327.1 CAbud0002_IIF_B12 Vitis vinifera cv. cabernet sauvignon (Clone 8) Bud - CABUD Vitis vinifera cDNA clone CAbud0002_IIF_B12 5', mRNA sequence.    48   1e-05  4
BE055424|BE055424.2 GA__Ea0003A10f Gossypium arboreum 7-10 dpa fiber library Gossypium arboreum cDNA clone GA__Ea0003A10f, mRNA sequence.    62   1e-05  1
CB823338|CB823338.1 EST 4562 Ripe Apricot Fruit Lambda Zap II Library Prunus armeniaca cDNA clone bh006b01 3', mRNA sequence.    62   1e-05  1
CB820052|CB820052.1 EST 1044 Green Apricot Fruit Lambda Zap II Library Prunus armeniaca cDNA clone ba004o03 3', mRNA sequence.    62   1e-05  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86AD9) RecName: Full=Probable acetyl-CoA acetyltransferase;   ...   379   e-104
D89184_1(D89184|pid:none) Schizosaccharomyces pombe mRNA, partia...   219   1e-55
BT071305_1(BT071305|pid:none) Picea sitchensis clone WS02822_K16...   197   5e-49
BT040119_1(BT040119|pid:none) Zea mays full-length cDNA clone ZM...   192   1e-47
EU962617_1(EU962617|pid:none) Zea mays clone 244258 acetyl-CoA a...   192   1e-47
(Q8S4Y1) RecName: Full=Acetyl-CoA acetyltransferase, cytosolic 1...   192   2e-47
AP007174_12(AP007174|pid:none) Aspergillus oryzae RIB40 genomic ...   191   4e-47
AY748246_1(AY748246|pid:none) Nicotiana tabacum peroxisomal acet...   191   4e-47
BT042208_1(BT042208|pid:none) Zea mays full-length cDNA clone ZM...   190   5e-47
FB905633_1(FB905633|pid:none) Sequence 124906 from Patent WO2008...   190   5e-47

 psg: 0.70  gvh: 0.48  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.40  mip: 0.09
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 24.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall

>> prediction for VFH459 is nuc

>VFH459F.Seq
AAAAATNATACACACACCAAAATATAAAATATATACTTAAAAAGATGATTTCAAACCTTT
CAAAAGTTTTAAACTCAAATGTAAAGAGAATGTATACTACAGCAAAAAACCTTGAATCAG
TTGTAATTGTTTCAGCAGTTAGAACACCAATTGGTTCAATTGGTGGTTCATTATCAACAA
TACCAGGTACAAAATTATCATCAATAACAATTGAAGAAGCAGTAAAGAGAGCAGGTATTA
AACCATCAGATGTTGATGAAGCAATCATTGGTAATGTAATTTCAGCCAATTTAGGACAAG
CACCAGCACGTCAATGTGCATTGGGAGCTGGTTTAGAACAAAAAACCATCACAACCACCA
TCAATAAAGTATGTTCATCAGGTATGAAAGCAATTGTATTTGGCGCACAATCAATCGCAT
TGGGTCACAGCAAAACTGTGGTTGCAGGTGGTTTCGAATCAATGTCACAAGTGCCATACT
ATGCCGATAAAATGAGATTCGGTGCAAAGTATGGCAACCAAACATTCATCGATGGTTTGG
TTAGAGATGGTTTAGCCGACGCATACAACGGAAGTGCTATGGGTGTTTGTGGAGATGATT
GTGCCGATAAATATAAGATCACTCGTG----------

>VFH459Z.Seq
----------CTTTGAAATCAATGAAGCCTTTGCTGTTGTTGGTCTTGCAAATGCAAAGA
TCTTAGATATCGATCATAATAAACTCAATGTTAATGGTGGTGCCGTTGCATTAGGTCATC
CAATTGGTTCATCAGGTTGTCGTATTGTTGTTACTCTTACTCATTTACTTCAAAATAAAA
ATCTTAAATATGGTGTTGCTGCTATTTGTAATGGTGGTGGAGGTAGTACTGCTTTAGTTT
TAGAAAAATTATAAATA

>VFH459P.Seq
AAAAATNATACACACACCAAAATATAAAATATATACTTAAAAAGATGATTTCAAACCTTT
CAAAAGTTTTAAACTCAAATGTAAAGAGAATGTATACTACAGCAAAAAACCTTGAATCAG
TTGTAATTGTTTCAGCAGTTAGAACACCAATTGGTTCAATTGGTGGTTCATTATCAACAA
TACCAGGTACAAAATTATCATCAATAACAATTGAAGAAGCAGTAAAGAGAGCAGGTATTA
AACCATCAGATGTTGATGAAGCAATCATTGGTAATGTAATTTCAGCCAATTTAGGACAAG
CACCAGCACGTCAATGTGCATTGGGAGCTGGTTTAGAACAAAAAACCATCACAACCACCA
TCAATAAAGTATGTTCATCAGGTATGAAAGCAATTGTATTTGGCGCACAATCAATCGCAT
TGGGTCACAGCAAAACTGTGGTTGCAGGTGGTTTCGAATCAATGTCACAAGTGCCATACT
ATGCCGATAAAATGAGATTCGGTGCAAAGTATGGCAACCAAACATTCATCGATGGTTTGG
TTAGAGATGGTTTAGCCGACGCATACAACGGAAGTGCTATGGGTGTTTGTGGAGATGATT
GTGCCGATAAATATAAGATCACTCGTG----------CTTTGAAATCAATGAAGCCTTTG
CTGTTGTTGGTCTTGCAAATGCAAAGATCTTAGATATCGATCATAATAAACTCAATGTTA
ATGGTGGTGCCGTTGCATTAGGTCATCCAATTGGTTCATCAGGTTGTCGTATTGTTGTTA
CTCTTACTCATTTACTTCAAAATAAAAATCTTAAATATGGTGTTGCTGCTATTTGTAATG
GTGGTGGAGGTAGTACTGCTTTAGTTTTAGAAAAATTATAAATA