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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFH291
Library VF
(Link to library)
Clone ID VFH291
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15456-1
Original site URL
Representative seq. ID VFH291P
(Link to Original site)
Representative DNA sequence 
>VFH291 (VFH291Q) /CSM/VF/VFH2-D/VFH291Q.Seq.d/
GTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACT
ATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCT
AGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACCACA
ATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCA
GGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAG
AAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCA
AGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCA
GGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTATTC
GCTGAAGCCACTGCCGAACCAGGTATTGCTTXXXXXXXXXXCCCATTAACCAACGAAACC
TATTGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGT
ACTACTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGGTCCAATGGCTGAT
ATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCCTTAAATGGTGAAGGTGTCTTCTC
CTGATTGTAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAAT
TTGTTTTAACCCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTT
TGAGTGGATTTATGGGTATCGAGTTAAACATGGGAAATTTNCTGGANCCTTGGTGATGTT
TT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
vt*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGTT
IPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGAS
STYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAEATAEPGIA---

---PLTNETYWEFVMDDFAIDGQSAGFCGTTCHAICDSGTSLIAGPMADITALNEKLGAV
ILKW*rclllivalstpyqmlpspllvvnlf*pqkntf*kllsserlnv*vdlwvss*tw
eixwxlgdv
Translated Amino Acid sequence (All Frames) 
Frame A:
vt*lt*KKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGTT
IPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGAS
STYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAEATAEPGIA---

---pinqrnllgiryg*lcyrwsiswflwyylsrnlrfryithcwsng*yycpq*kircc
hp*mvkvsspdcsvintlpnvtitvagrefvltpkeyvlevtefgkteclsgfmgielnm
gnxlxpw*cf

Frame B:
spdlhkrk*nylf*lyf*lllf*lkl*qyh*tsiklqenleeefhkngqtdyllsmlvpq
sqfqilkmlntmvplplvpqvkpsk*fsilvhptcgfhqrnvqslllhviyitnitavpq
ahmsptelispsntvvvlcqvlslkipsllvh*llkinyslkplpnqvll---

---PLTNETYWEFVMDDFAIDGQSAGFCGTTCHAICDSGTSLIAGPMADITALNEKLGAV
ILKW*rclllivalstpyqmlpspllvvnlf*pqkntf*kllsserlnv*vdlwvss*tw
eixwxlgdv

Frame C:
hltyikeneityfnfifsyycfsssfnstiklpssfkri*kksstkmvkqiicsqcwyhn
pnfrf*rcsilwchyhwyprsslqssfrywfiqlvdsikemsnhcccm*ft*qi*qrclk
hicrqrn*fhhpir*wcyvrfclsrfrhcwfinc*rsiir*shcrtryc---

---h*ptkpignslwmtllsmvnqlvsvvllvtqfaiqvhhsllvqwlillpsmkn*vls
slngegvfs*l*ryqhltkcyhhrcws*icfnpkrirfrsy*vrkd*mfewiygyrvkhg
kfxgxlvmf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO689 (VFO689Q) /CSM/VF/VFO6-D/VFO689Q.Seq.d/                       1013   0.0
VFN875 (VFN875Q) /CSM/VF/VFN8-D/VFN875Q.Seq.d/                       1013   0.0
VFN780 (VFN780Q) /CSM/VF/VFN7-D/VFN780Q.Seq.d/                       1013   0.0
VFN696 (VFN696Q) /CSM/VF/VFN6-D/VFN696Q.Seq.d/                       1013   0.0
VFN650 (VFN650Q) /CSM/VF/VFN6-C/VFN650Q.Seq.d/                       1013   0.0
VFN467 (VFN467Q) /CSM/VF/VFN4-C/VFN467Q.Seq.d/                       1013   0.0
VFN222 (VFN222Q) /CSM/VF/VFN2-A/VFN222Q.Seq.d/                       1013   0.0
VFN169 (VFN169Q) /CSM/VF/VFN1-C/VFN169Q.Seq.d/                       1013   0.0
VFM881 (VFM881Q) /CSM/VF/VFM8-D/VFM881Q.Seq.d/                       1013   0.0
VFM829 (VFM829Q) /CSM/VF/VFM8-B/VFM829Q.Seq.d/                       1013   0.0
own update 2001.11.28
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

Y16962|Y16962.1 Dictyostelium discoideum mRNA for cathepsin D.   1007   0.0    5
AJ243946|AJ243946.1 Dictyostelium discoideum ctsD gene for cathepsin D, exons 1 to 2.   848   0.0    6
CD825453|CD825453.1 BN25.060N03F011129 BN25 Brassica napus cDNA clone BN25060N03, mRNA sequence.    62   1e-05  1
BX838383|BX838383.1 Arabidopsis thaliana Full-length cDNA 5PRIM end of clone  GSLTSIL89ZD03 of Silique of strain col-0 of Arabidopsis thaliana  (thale cress).    62   1e-05  1
AF076243|AF076243.1 Arabidopsis thaliana BAC T26N6 from chromosome IV at 19.3 cM, complete sequence.    62   1e-05  1
BX827174|BX827174.1 Arabidopsis thaliana Full-length cDNA Complete sequence from clone  GSLTLS1ZB10 of Adult vegetative tissue of strain col-0 of  Arabidopsis thaliana (thale cress).    62   1e-05  1
BX829292|BX829292.1 Arabidopsis thaliana Full-length cDNA Complete sequence from clone  GSLTSIL89ZE03 of Silique of strain col-0 of Arabidopsis thaliana  (thale cress).    62   1e-05  1
AV567523|AV567523.1 Arabidopsis thaliana cDNA clone:SQL15g03F, 3' end.    62   1e-05  1
CB264640|CB264640.1 48-E014661-035-002-P12-T7R MPIZ-ADIS-035 Arabidopsis thaliana cDNA clone MPIZp2000P122Q 5-PRIME, mRNA sequence.    62   1e-05  1
CB264579|CB264579.1 51-E015023-035-004-F13-T7R MPIZ-ADIS-035 Arabidopsis thaliana cDNA clone MPIZp2000F134Q 5-PRIME, mRNA sequence.    62   1e-05  1
dna update 2004. 2.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ010007_1(DQ010007|pid:none) Bombyx mori CathD mRNA, complete c...   162   2e-38
AF454831_1(AF454831|pid:none) Apriona germari cathepsin D mRNA, ...   161   2e-38
FJ654712_1(FJ654712|pid:none) Chrysomela tremulae aspartic prote...   161   3e-38
EF213114_1(EF213114|pid:none) Penaeus monodon cathepsin D mRNA, ...   160   7e-38
AB106552_1(AB106552|pid:none) Todarodes pacificus tpaD mRNA for ...   159   9e-38
EF070454_1(EF070454|pid:none) Maconellicoccus hirsutus clone WHM...   157   3e-37
CP001577_36(CP001577|pid:none) Micromonas sp. RCC299 chromosome ...   157   5e-37
(Q03168) RecName: Full=Lysosomal aspartic protease;          EC=...   154   3e-36
T47207(T47207) aspartic proteinase (EC 3.4.23.-) [imported] - Ne...   152   1e-35
(Q01294) RecName: Full=Vacuolar protease A;          EC=3.4.23.-...   152   1e-35
protein update 2009. 6.20
PSORT 

 psg: 0.98  gvh: 0.77  alm: 0.46  top: 0.57  tms: 0.00  mit: 0.36  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: extracellular, including cell wall
	 12.0 %: nuclear
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoplasmic
	  4.0 %: Golgi
	  4.0 %: mitochondrial

>> prediction for VFH291 is exc
5' end seq. ID VFH291F
5' end seq. 
>VFH291F.Seq
GTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACT
ATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCT
AGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACCACA
ATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCA
GGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAG
AAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCA
AGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCA
GGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTATTC
GCTGAAGCCACTGCCGAACCAGGTATTGCTT----------
Length of 5' end seq. 511
3' end seq. ID VFH291Z
3' end seq. 
>VFH291Z.Seq
----------CCCATTAACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTA
TCGATGGTCAATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTA
CATCACTCATTGCTGGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTG
TCATCCTTAAATGGTGAAGGTGTCTTCTCCTGATTGTAGCGTTATCAACACCTTACCAAA
TGTTACCATCACCGTTGCTGGTCGTGAATTTGTTTTAACCCCAAAAGAATACGTTTTAGA
AGTTACTGAGTTCGGAAAGACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACAT
GGGAAATTTNCTGGANCCTTGGTGATGTTTT
Length of 3' end seq. 381
Connected seq. ID VFH291P
Connected seq. 
>VFH291P.Seq
GTCACCTGACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACT
ATTGTTTTAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCT
AGAAGAAGAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACCACA
ATCCCAATTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCA
GGTCAAGCCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAG
AAATGTCCAATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCA
AGCACATATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCA
GGTTTTGTCTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTATTC
GCTGAAGCCACTGCCGAACCAGGTATTGCTT----------CCCATTAACCAACGAAACC
TATTGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGT
ACTACTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGGTCCAATGGCTGAT
ATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCCTTAAATGGTGAAGGTGTCTTCTC
CTGATTGTAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAAT
TTGTTTTAACCCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTT
TGAGTGGATTTATGGGTATCGAGTTAAACATGGGAAATTTNCTGGANCCTTGGTGATGTT
TT
Length of connected seq. 892
Full length Seq ID -
Full length Seq. -
Length of full length seq. -