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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFH286
Library VF
(Link to library)
Clone ID VFH286
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16182-1
Original site URL
Representative seq. ID VFH286P
(Link to Original site)
Representative DNA sequence 
>VFH286 (VFH286Q) /CSM/VF/VFH2-D/VFH286Q.Seq.d/
TAAAAAAATAAACTCATGTACATATAAAAATTAAAATAAAAGAAAAAAAAATGAAAAGGA
CGATAATAATATTATTATTTGTATCATTATTTGTAACATTTGTTTTATCACAAATACCAG
ATAATGATCCAGAATTATGTCAACAAAGAATTCAAAGAGAGGATTGTCCACAAACACCAG
TACCATGGGGAACATTCAATGACAATGACGAAATCGAAGTCTACTATATGCAAGCACCAG
TTTTCGAAGCAGTTTTTGGAAATTTCTTTGGTAAGTTGGGTGGATATCATTCAGCAATTG
GTTTCTATGACCTTACAACTGGTTTAAATTATACAGCAGAATATGATGCTTTCTTTGAAG
TAGGAAATGGTACATTACCAAATATCATAAATGTCGATGGTAAAGATGAACTTTTATGGT
GTAATGCTGGTATCCTTTGTACTGTTCCATACATTAATGAAACTTATTGGGATAAAAACA
TCTATTCAACTGCTTCAAAAACTTATXXXXXXXXXXANAAATTAAANAAGGCNCAATTGC
CCAAATCNTTGAAANAATTTTATCAATCNTTGGTTTAAANAAANATATTTACGTTCAAGG
TGAATATTNTTTATTANAATTAAATTATCCNTTTATGAATGCAAAATATGATTATNTCNC
NTTACCAGGTTGGCCAGCAAATGGNATCGATTATGAAAATGGCNATTTTATTCATCCCAA
TTAGATTATTTTAAAATTAGATTATTTTAAATTAAATTGGTTATAAATAAAAACNAAAAA
CNAAAATAATAAAAAAAAAATNNCTTTTTTTTTTAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
kk*THVHIKIKIKEKKMKRTIIILLFVSLFVTFVLSQIPDNDPELCQQRIQREDCPQTPV
PWGTFNDNDEIEVYYMQAPVFEAVFGNFFGKLGGYHSAIGFYDLTTGLNYTAEYDAFFEV
GNGTLPNIINVDGKDELLWCNAGILCTVPYINETYWDKNIYSTASKTY---

---XIKXGXIAQIXEXILSIXGLXKXIYVQGEYXLLXLNYPFMNAKYDYXXLPGWPANGI
DYENGXFIHPN*iilkldyfklnwl*iktknxnnkkkxxfff
Translated Amino Acid sequence (All Frames) 
Frame A:
*knklmyi*klk*kkkk*kgr**yyylyhyl*hlfyhkyqimiqnyvnkefkerivhkhq
yhgehsmtmtkskstickhqfskqfleislvswvdiiqqlvsmtlqlv*iiqqnmmlslk
*emvhyqis*msmvkmnfygvmlvsfvlfhtlmkligiktsiqllqkl---

---xn*xrxncpnx*xnfinxwfkxxylrsr*ixfixiklsxyecki*lxxxtrlaskwx
rl*kwxfyssqldyfkirlf*iklvinknxkxk**kknxfff*

Frame B:
kkinsctykn*nkrkknekddnniiiciiicnicfitntr**srimstknskrglstnts
tmgniq*q*rnrsllyastsfrssfwkflw*vgwisfsnwfl*pynwfklysri*cfl*s
rkwyitkyhkcrw*r*tfmv*cwyplycsih**nllg*khlfncfknl---

---XIKXGXIAQIXEXILSIXGLXKXIYVQGEYXLLXLNYPFMNAKYDYXXLPGWPANGI
DYENGXFIHPN*iilkldyfklnwl*iktknxnnkkkxxfff

Frame C:
kk*THVHIKIKIKEKKMKRTIIILLFVSLFVTFVLSQIPDNDPELCQQRIQREDCPQTPV
PWGTFNDNDEIEVYYMQAPVFEAVFGNFFGKLGGYHSAIGFYDLTTGLNYTAEYDAFFEV
GNGTLPNIINVDGKDELLWCNAGILCTVPYINETYWDKNIYSTASKTY---

---klxkaqlpkslkxfyqslv*xxiftfkvnixyxn*iixl*mqnmixsxyqvgqqmxs
imkmailfipirlf*n*iiln*igyk*kxktkiikkkxlffl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFH286 (VFH286Q) /CSM/VF/VFH2-D/VFH286Q.Seq.d/                       1128   0.0
VHL857 (VHL857Q) /CSM/VH/VHL8-C/VHL857Q.Seq.d/                        890   0.0
VFL220 (VFL220Q) /CSM/VF/VFL2-A/VFL220Q.Seq.d/                        890   0.0
VFJ281 (VFJ281Q) /CSM/VF/VFJ2-D/VFJ281Q.Seq.d/                        890   0.0
VFG895 (VFG895Q) /CSM/VF/VFG8-D/VFG895Q.Seq.d/                        890   0.0
SSA660 (SSA660Q) /CSM/SS/SSA6-C/SSA660Q.Seq.d/                        890   0.0
VFC480 (VFC480Q) /CSM/VF/VFC4-D/VFC480Q.Seq.d/                        862   0.0
SSB231 (SSB231Q) /CSM/SS/SSB2-B/SSB231Q.Seq.d/                        821   0.0
SFJ661 (SFJ661Q) /CSM/SF/SFJ6-C/SFJ661Q.Seq.d/                        769   0.0
SSG340 (SSG340Q) /CSM/SS/SSG3-B/SSG340Q.Seq.d/                        676   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   890   0.0    7
AC123538|AC123538.3 Sminthopsis macroura clone 79G10, complete sequence.    42   0.003  4
AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    44   0.008  7
AL034557|AL034557.8 Plasmodium falciparum MAL4P1.                  52   0.013  1
AL844507|AL844507.1 Plasmodium falciparum chromosome 8.            36   0.097  12
BX324188|BX324188.6 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    38   0.13   6
AC116101|AC116101.2 Dictyostelium discoideum chromosome 2 map complement(427476-387492) strain AX4, complete sequence.    42   0.18   4
AC133307|AC133307.2 Rattus norvegicus clone CH230-269I2, WORKING DRAFT SEQUENCE, 3 unordered pieces.    48   0.20   1
AC126501|AC126501.4 Rattus norvegicus clone CH230-206K3, WORKING DRAFT SEQUENCE, 2 unordered pieces.    48   0.20   1
BH334420|BH334420.1 CH230-110K10.TJ CHORI-230 Segment 1 Rattus norvegicus genomic clone CH230-110K10, DNA sequence.    48   0.20   1
dna update 2004. 2.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q553W9) RecName: Full=Ceroid-lipofuscinosis neuronal protein 5 ...   289   9e-77
AC116305_28(AC116305|pid:none) Dictyostelium discoideum chromoso...   289   9e-77
AK032293_1(AK032293|pid:none) Mus musculus adult male olfactory ...    79   2e-13
AF068227_1(AF068227|pid:none) Homo sapiens putative transmembran...    79   2e-13
BC025487_1(BC025487|pid:none) Mus musculus ceroid-lipofuscinosis...    79   2e-13
AK075109_1(AK075109|pid:none) Homo sapiens cDNA FLJ90628 fis, cl...    77   9e-13
AY885123_1(AY885123|pid:none) Canis familiaris CLN5 (CLN5) gene,...    77   9e-13
(Q1ZYR0) RecName: Full=Ceroid-lipofuscinosis neuronal protein 5;...    77   9e-13
(Q5JZQ9) Ceroid-lipofuscinosis neuronal protein 5 precursor (Pro...    77   9e-13
(A2TJ54) RecName: Full=Ceroid-lipofuscinosis neuronal protein 5;...    76   1e-12
protein update 2009. 6.20
PSORT 

 psg: 0.47  gvh: 0.86  alm: 0.17  top: -0.13  tms: 0.07  mit: 0.25  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 20.0 %: nuclear
	 16.0 %: mitochondrial
	 16.0 %: Golgi
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for VFH286 is cyt
5' end seq. ID VFH286F
5' end seq. 
>VFH286F.Seq
TAAAAAAATAAACTCATGTACATATAAAAATTAAAATAAAAGAAAAAAAAATGAAAAGGA
CGATAATAATATTATTATTTGTATCATTATTTGTAACATTTGTTTTATCACAAATACCAG
ATAATGATCCAGAATTATGTCAACAAAGAATTCAAAGAGAGGATTGTCCACAAACACCAG
TACCATGGGGAACATTCAATGACAATGACGAAATCGAAGTCTACTATATGCAAGCACCAG
TTTTCGAAGCAGTTTTTGGAAATTTCTTTGGTAAGTTGGGTGGATATCATTCAGCAATTG
GTTTCTATGACCTTACAACTGGTTTAAATTATACAGCAGAATATGATGCTTTCTTTGAAG
TAGGAAATGGTACATTACCAAATATCATAAATGTCGATGGTAAAGATGAACTTTTATGGT
GTAATGCTGGTATCCTTTGTACTGTTCCATACATTAATGAAACTTATTGGGATAAAAACA
TCTATTCAACTGCTTCAAAAACTTAT----------
Length of 5' end seq. 506
3' end seq. ID VFH286Z
3' end seq. 
>VFH286Z.Seq
----------ANAAATTAAANAAGGCNCAATTGCCCAAATCNTTGAAANAATTTTATCAA
TCNTTGGTTTAAANAAANATATTTACGTTCAAGGTGAATATTNTTTATTANAATTAAATT
ATCCNTTTATGAATGCAAAATATGATTATNTCNCNTTACCAGGTTGGCCAGCAAATGGNA
TCGATTATGAAAATGGCNATTTTATTCATCCCAATTAGATTATTTTAAAATTAGATTATT
TTAAATTAAATTGGTTATAAATAAAAACNAAAAACNAAAATAATAAAAAAAAAATNNCTT
TTTTTTTTAA
Length of 3' end seq. 300
Connected seq. ID VFH286P
Connected seq. 
>VFH286P.Seq
TAAAAAAATAAACTCATGTACATATAAAAATTAAAATAAAAGAAAAAAAAATGAAAAGGA
CGATAATAATATTATTATTTGTATCATTATTTGTAACATTTGTTTTATCACAAATACCAG
ATAATGATCCAGAATTATGTCAACAAAGAATTCAAAGAGAGGATTGTCCACAAACACCAG
TACCATGGGGAACATTCAATGACAATGACGAAATCGAAGTCTACTATATGCAAGCACCAG
TTTTCGAAGCAGTTTTTGGAAATTTCTTTGGTAAGTTGGGTGGATATCATTCAGCAATTG
GTTTCTATGACCTTACAACTGGTTTAAATTATACAGCAGAATATGATGCTTTCTTTGAAG
TAGGAAATGGTACATTACCAAATATCATAAATGTCGATGGTAAAGATGAACTTTTATGGT
GTAATGCTGGTATCCTTTGTACTGTTCCATACATTAATGAAACTTATTGGGATAAAAACA
TCTATTCAACTGCTTCAAAAACTTAT----------ANAAATTAAANAAGGCNCAATTGC
CCAAATCNTTGAAANAATTTTATCAATCNTTGGTTTAAANAAANATATTTACGTTCAAGG
TGAATATTNTTTATTANAATTAAATTATCCNTTTATGAATGCAAAATATGATTATNTCNC
NTTACCAGGTTGGCCAGCAAATGGNATCGATTATGAAAATGGCNATTTTATTCATCCCAA
TTAGATTATTTTAAAATTAGATTATTTTAAATTAAATTGGTTATAAATAAAAACNAAAAA
CNAAAATAATAAAAAAAAAATNNCTTTTTTTTTTAA
Length of connected seq. 806
Full length Seq ID -
Full length Seq. -
Length of full length seq. -