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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VFH250
Library VF
(Link to library)
Clone ID VFH250
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15170-1
Original site URL
Representative seq. ID VFH250P
(Link to Original site)
Representative DNA sequence 
>VFH250 (VFH250Q) /CSM/VF/VFH2-C/VFH250Q.Seq.d/
ACTGTTGGCCTACTGGNAAATACTACCCACACACATNCAGTCATATAACCAAAAAAACAT
AAATACATAAATGGCATCTACTACAACTACAATTCTACCAAAAGATCATATCAAATTAAG
ATCAACTATACATCAAAAACTTATAGAATCAGGAGAAAAAGAAAGATTAAAGGTTTTATT
AAAGAGTAAATTAGTTGAAGGTGGTTGGAGAGATGAAGTTTTXXXXXXXXXXTAAGTAAA
AATTGCNTGTCGTGAATATATTNAAAATAATCCAAATNAAAACNTTAAAATTNAANATTT
NATTGCCCNCANAACNCCNANTGCNAAAAAAAAAAGNACCNCCNCNAGTTAAAGCAGATT
TAATTAANAGAATTAGAAAATTTTTAGGCCCAAATATTCAAAATCATCATCATCATCATA
TCCATATTCA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
llaywxilpthxqsynqknint*MASTTTTILPKDHIKLRSTIHQKLIESGEKERLKVLL
KSKLVEGGWRDEV---

---SKNCXS*iyxk*skxkx*nxxfxcpxnxxcxkkkxxxxlkqi*lxelenf*aqifki
iiiiisif
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxntththxvi*pkkhkyingiyynynstkrsyqikinytsktyrirrkrkikgfi
ke*is*rwler*sf---

---*vkiacreyixnnpnxnxkixxxiaxxtpxakkkxtxxs*srfn*xn*kifrpkysk
sssssypys

Frame B:
llaywxilpthxqsynqknint*MASTTTTILPKDHIKLRSTIHQKLIESGEKERLKVLL
KSKLVEGGWRDEV---

---k*klxvvnixkiiqxktlkxxixlpxxxxxxkkkxppxvkadlixrirkflgpniqn
hhhhhihi

Frame C:
cwptgkyyphtxshitkkt*ihkwhllqlqfyqkiisn*dqlyiknl*nqekkkd*rfy*
rvn*lkvvgemkf---

---SKNCXS*iyxk*skxkx*nxxfxcpxnxxcxkkkxxxxlkqi*lxelenf*aqifki
iiiiisif
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFH250 (VFH250Q) /CSM/VF/VFH2-C/VFH250Q.Seq.d/                        517   e-146
SSC488 (SSC488Q) /CSM/SS/SSC4-D/SSC488Q.Seq.d/                        343   1e-93
VSF495 (VSF495Q) /CSM/VS/VSF4-D/VSF495Q.Seq.d/                        321   5e-87
FC-BA17 (FC-BA17Q) /CSM/FC/FC-BA/FC-BA17Q.Seq.d/                      321   5e-87
SSG412 (SSG412Q) /CSM/SS/SSG4-A/SSG412Q.Seq.d/                        147   2e-34
VFK587 (VFK587Q) /CSM/VF/VFK5-D/VFK587Q.Seq.d/                         42   0.007
SHK441 (SHK441Q) /CSM/SH/SHK4-B/SHK441Q.Seq.d/                         42   0.007
CFC670 (CFC670Q) /CSM/CF/CFC6-C/CFC670Q.Seq.d/                         42   0.007
CHA742 (CHA742Q) /CSM/CH/CHA7-B/CHA742Q.Seq.d/                         40   0.027
CFJ884 (CFJ884Q) /CSM/CF/CFJ8-D/CFJ884Q.Seq.d/                         40   0.027
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.    32   0.14   5
AC012449|AC012449.7 Homo sapiens BAC clone RP11-321K13 from 2, complete sequence.    40   0.34   3
BI745035|BI745035.1 rk95d09.y1 Meloidogyne javanica egg pAMP1 v6 Chiapelli McCarter Meloidogyne javanica cDNA 5' similar to SW:YR44_CAEEL Q09390 HYPOTHETICAL 23.8 KD PROTEIN F47D12.4 IN CHROMOSOME III. [2] TR:O77096 ;, mRNA sequence.    46   0.39   1
BM882482|BM882482.1 rb02b12.y1 Meloidogyne incognita egg pAMP1 Bird Meloidogyne incognita cDNA 5' similar to SW:YR44_CAEEL Q09390 HYPOTHETICAL 23.8 KD PROTEIN F47D12.4 IN CHROMOSOME III. [2] TR:O77096 ;, mRNA sequence.    46   0.39   1
BM774872|BM774872.1 ra98f07.y1 Meloidogyne incognita egg pAMP1 Bird Meloidogyne incognita cDNA 5' similar to SW:YR44_CAEEL Q09390 HYPOTHETICAL 23.8 KD PROTEIN F47D12.4 IN CHROMOSOME III. [2] TR:O77096 ;, mRNA sequence.    46   0.39   1
BI863039|BI863039.1 rm15g01.y1 Meloidogyne arenaria egg pAMP1 v1 Chiapelli McCarter Meloidogyne arenaria cDNA 5' similar to SW:YR44_CAEEL Q09390 HYPOTHETICAL 23.8 KD PROTEIN F47D12.4 IN CHROMOSOME III. [2] TR:O77096 ;, mRNA sequence.    46   0.39   1
BM773792|BM773792.1 ra86a02.y1 Meloidogyne incognita egg pAMP1 Bird Meloidogyne incognita cDNA 5' similar to SW:YR44_CAEEL Q09390 HYPOTHETICAL 23.8 KD PROTEIN F47D12.4 IN CHROMOSOME III. [2] TR:O77096 ;, mRNA sequence.    46   0.39   1
BM773831|BM773831.1 ra86d08.y1 Meloidogyne incognita egg pAMP1 Bird Meloidogyne incognita cDNA 5' similar to SW:YR44_CAEEL Q09390 HYPOTHETICAL 23.8 KD PROTEIN F47D12.4 IN CHROMOSOME III. [2] TR:O77096 ;, mRNA sequence.    46   0.39   1
BM882351|BM882351.1 ra99g01.y1 Meloidogyne incognita egg pAMP1 Bird Meloidogyne incognita cDNA 5' similar to SW:YR44_CAEEL Q09390 HYPOTHETICAL 23.8 KD PROTEIN F47D12.4 IN CHROMOSOME III. [2] TR:O77096 ;, mRNA sequence.    46   0.39   1
BM880516|BM880516.1 rb23b12.y1 Meloidogyne incognita egg pAMP1 Bird Meloidogyne incognita cDNA 5' similar to SW:YR44_CAEEL Q09390 HYPOTHETICAL 23.8 KD PROTEIN F47D12.4 IN CHROMOSOME III. [2] TR:O77096 ;, mRNA sequence.    46   0.39   1
dna update 2004. 1.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK175619_1(AK175619|pid:none) Arabidopsis thaliana mRNA for hypo...    59   4e-08
AK120182_1(AK120182|pid:none) Oryza sativa Japonica Group cDNA c...    58   9e-08
(B5FZ63) RecName: Full=Enhancer of yellow 2 transcription factor...    57   2e-07
AX885677_1(AX885677|pid:none) Sequence 1540 from Patent EP1033401.     57   3e-07
(Q3ZBJ0) RecName: Full=Enhancer of yellow 2 transcription factor...    57   3e-07
AX885678_1(AX885678|pid:none) Sequence 1541 from Patent EP1033401.     56   4e-07
(Q3KPT5) RecName: Full=Enhancer of yellow 2 transcription factor...    55   6e-07
(B2RYZ5) RecName: Full=Enhancer of yellow 2 transcription factor...    55   6e-07
(B5XGH3) RecName: Full=Enhancer of yellow 2 transcription factor...    55   1e-06
AB026649_17(AB026649|pid:none) Arabidopsis thaliana genomic DNA,...    54   1e-06
protein update 2009. 6.20
PSORT 

 psg: 0.49  gvh: 0.41  alm: 0.62  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 32.0 %: mitochondrial
	 24.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for VFH250 is nuc
5' end seq. ID VFH250F
5' end seq. 
>VFH250F.Seq
ACTGTTGGCCTACTGGNAAATACTACCCACACACATNCAGTCATATAACCAAAAAAACAT
AAATACATAAATGGCATCTACTACAACTACAATTCTACCAAAAGATCATATCAAATTAAG
ATCAACTATACATCAAAAACTTATAGAATCAGGAGAAAAAGAAAGATTAAAGGTTTTATT
AAAGAGTAAATTAGTTGAAGGTGGTTGGAGAGATGAAGTTTT----------
Length of 5' end seq. 222
3' end seq. ID VFH250Z
3' end seq. 
>VFH250Z.Seq
----------TAAGTAAAAATTGCNTGTCGTGAATATATTNAAAATAATCCAAATNAAAA
CNTTAAAATTNAANATTTNATTGCCCNCANAACNCCNANTGCNAAAAAAAAAAGNACCNC
CNCNAGTTAAAGCAGATTTAATTAANAGAATTAGAAAATTTTTAGGCCCAAATATTCAAA
ATCATCATCATCATCATATCCATATTCA
Length of 3' end seq. 198
Connected seq. ID VFH250P
Connected seq. 
>VFH250P.Seq
ACTGTTGGCCTACTGGNAAATACTACCCACACACATNCAGTCATATAACCAAAAAAACAT
AAATACATAAATGGCATCTACTACAACTACAATTCTACCAAAAGATCATATCAAATTAAG
ATCAACTATACATCAAAAACTTATAGAATCAGGAGAAAAAGAAAGATTAAAGGTTTTATT
AAAGAGTAAATTAGTTGAAGGTGGTTGGAGAGATGAAGTTTT----------TAAGTAAA
AATTGCNTGTCGTGAATATATTNAAAATAATCCAAATNAAAACNTTAAAATTNAANATTT
NATTGCCCNCANAACNCCNANTGCNAAAAAAAAAAGNACCNCCNCNAGTTAAAGCAGATT
TAATTAANAGAATTAGAAAATTTTTAGGCCCAAATATTCAAAATCATCATCATCATCATA
TCCATATTCA
Length of connected seq. 420
Full length Seq ID -
Full length Seq. -
Length of full length seq. -