>VFH244 (VFH244Q) /CSM/VF/VFH2-B/VFH244Q.Seq.d/
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCANATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACCTTTGGTCATXXXXXX
XXXX

nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPXVCGASKRVHVILNYKGKNHLIKK
EINKVETDQLTHQYTFG---

Frame A:
nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPXVCGASKRVHVILNYKGKNHLIKK
EINKVETDQLTHQYTFG---


Frame B:
ispiiifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllv
igikkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*r
mnkklivvvhtssyyhpn*inqhsmvnlniplclvxmfvvpqreftsfsttkerti*srk
kstklkpin*lintplv---


Frame C:
flqssyl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**
la*rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke
*tks*lwwfihqvitiqirsisirw*i*ifhyvwsxclwclkessrhsqlqrkepfnqer
nqqs*nrsinssihlws---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFH244 (VFH244Q) /CSM/VF/VFH2-B/VFH244Q.Seq.d/                        946   0.0
VFE283 (VFE283Q) /CSM/VF/VFE2-D/VFE283Q.Seq.d/                        946   0.0
AFM286 (AFM286Q) /CSM/AF/AFM2-D/AFM286Q.Seq.d/                        946   0.0
AFL648 (AFL648Q) /CSM/AF/AFL6-B/AFL648Q.Seq.d/                        940   0.0
AFM225 (AFM225Q) /CSM/AF/AFM2-B/AFM225Q.Seq.d/                        938   0.0
AFK156 (AFK156Q) /CSM/AF/AFK1-C/AFK156Q.Seq.d/                        938   0.0
AFG656 (AFG656Q) /CSM/AF/AFG6-C/AFG656Q.Seq.d/                        938   0.0
AFE140 (AFE140Q) /CSM/AF/AFE1-B/AFE140Q.Seq.d/                        932   0.0
VFM435 (VFM435Q) /CSM/VF/VFM4-B/VFM435Q.Seq.d/                        930   0.0
VFM276 (VFM276Q) /CSM/VF/VFM2-D/VFM276Q.Seq.d/                        930   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.   926   0.0    2
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92   2e-22  3
BI876472|BI876472.1 fl72h01.y1 Sugano Kawakami zebrafish DRA Danio rerio cDNA clone 3819504 5' similar to TR:Q9PUC1 Q9PUC1 CALRETICULIN. ;, mRNA sequence.    42   3e-04  2
CB086220|CB086220.1 hj81f04.g1 Hedyotis centranthoides flower - Stage 2 (NYBG) Hedyotis centranthoides cDNA clone hj81f04, mRNA sequence.    36   3e-04  3
AY342298|AY342298.1 Ictalurus punctatus ER-resident chaperone calreticulin mRNA, complete cds.    56   6e-04  1
CB937139|CB937139.1 IpCGJx13_12_E07_23 IpCGJx13 Ictalurus punctatus cDNA clone IpCGJx13_12_E07 5', mRNA sequence.    56   6e-04  1
BI468042|BI468042.1 EST00452 Atlantic salmon Lambda Zap Express liver cDNA library Salmo salar cDNA clone LRR7-f02 5' similar to Calreticulin, mRNA sequence.    56   6e-04  1
CK420742|CK420742.1 AUF_IpTrk_27_j08 Trunk kidney cDNA library Ictalurus punctatus cDNA 5' similar to ER-resident chaperone calreticulin, mRNA sequence.    56   6e-04  1
BQ261502|BQ261502.1 fz71d12.y1 Sugano SJD adult male Danio rerio cDNA clone 5914030 5' similar to TR:Q9PUC1 Q9PUC1 CALRETICULIN. ;, mRNA sequence.    40   0.001  2
BM957414|BM957414.1 fy77g03.y1 Sugano SJD adult male Danio rerio cDNA clone 5777093 5' similar to TR:Q9PUC1 Q9PUC1 CALRETICULIN. ;, mRNA sequence.    40   0.001  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   327   2e-88
AK318734_1(AK318734|pid:none) Arabidopsis thaliana AT1G56340 mRN...   206   4e-52
U27698_1(U27698|pid:none) Arabidopsis thaliana calreticulin (AtC...   206   4e-52
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   206   4e-52
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   204   1e-51
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   204   2e-51
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   204   2e-51
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   204   2e-51
AY086745_1(AY086745|pid:none) Arabidopsis thaliana clone 27210 m...   203   3e-51
AC003114_8(AC003114|pid:none) Arabidopsis thaliana chromosome 1 ...   203   3e-51

 psg: 1.01  gvh: 0.69  alm: 0.46  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: nuclear
	  4.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for VFH244 is exc

>VFH244F.Seq
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCANATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACCTTTGGTCAT------
----