>VFH221 (VFH221Q) /CSM/VF/VFH2-A/VFH221Q.Seq.d/
TAAACAACCATGGGTAAAGAGAAAACTCACATTAACATTGTCGTCATTGGTCACGTAGAT
GCTGGTAAATCAACCACCACTGGTCATTTAATCTACAAATGNGGTGGTATCGATAAGAGA
GTTAXXXXXXXXXX

*TTMGKEKTHINIVVIGHVDAGKSTTTGHLIYKXGGIDKRV---

Frame A:
*TTMGKEKTHINIVVIGHVDAGKSTTTGHLIYKXGGIDKRV---


Frame B:
kqpwvkrkltltlsslvt*mlvnqpplvi*stnxvvsire---


Frame C:
nnhg*rensh*hcrhwsrrcw*inhhwsfnlqmxwyr*es---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI414 (VSI414Q) /CSM/VS/VSI4-A/VSI414Q.Seq.d/                        240   4e-63
VSG690 (VSG690Q) /CSM/VS/VSG6-D/VSG690Q.Seq.d/                        240   4e-63
VSD740 (VSD740Q) /CSM/VS/VSD7-B/VSD740Q.Seq.d/                        240   4e-63
VSD566 (VSD566Q) /CSM/VS/VSD5-C/VSD566Q.Seq.d/                        240   4e-63
VSD380 (VSD380Q) /CSM/VS/VSD3-D/VSD380Q.Seq.d/                        240   4e-63
VHO616 (VHO616Q) /CSM/VH/VHO6-A/VHO616Q.Seq.d/                        240   4e-63
VHF352 (VHF352Q) /CSM/VH/VHF3-C/VHF352Q.Seq.d/                        240   4e-63
VHE101 (VHE101Q) /CSM/VH/VHE1-A/VHE101Q.Seq.d/                        240   4e-63
VFO891 (VFO891Q) /CSM/VF/VFO8-D/VFO891Q.Seq.d/                        240   4e-63
VFO873 (VFO873Q) /CSM/VF/VFO8-D/VFO873Q.Seq.d/                        240   4e-63

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X55973|X55973.1 D. discoideum EF1-I gene for elongation factor 1 alpha.   204   2e-49  1
X55972|X55972.1 D. discoideum EF1-II gene for elongation factor 1 alpha.   151   3e-33  1
CA754741|0   129   1e-26  1
CF433174|CF433174.1 NIT1_25_D08.g1_A002 Nitrogen-deficient seedlings Sorghum bicolor cDNA clone NIT1_25_D08_A002 5', mRNA sequence.   125   2e-25  1
CF433248|CF433248.1 NIT1_25_H11.g1_A002 Nitrogen-deficient seedlings Sorghum bicolor cDNA clone NIT1_25_H11_A002 5', mRNA sequence.   125   2e-25  1
CF433932|CF433932.1 NIT1_31_E10.b1_A002 Nitrogen-deficient seedlings Sorghum bicolor cDNA clone NIT1_31_E10_A002 3', mRNA sequence.   125   2e-25  1
CF433089|CF433089.1 NIT1_20_G08.g1_A002 Nitrogen-deficient seedlings Sorghum bicolor cDNA clone NIT1_20_G08_A002 5', mRNA sequence.   125   2e-25  1
CF432603|CF432603.1 NIT1_17_A01.g1_A002 Nitrogen-deficient seedlings Sorghum bicolor cDNA clone NIT1_17_A01_A002 5', mRNA sequence.   125   2e-25  1
BQ563155|BQ563155.1 ar10-078 Artemia EII Stage, Stratagene (cat. 200450) Artemia franciscana cDNA clone ar10-078 5' similar to elongation factor-1 alpha, mRNA sequence.   123   6e-25  1
X03349|X03349.1 Brine shrimp (artemia) mRNA for elongation factor EF-1 alpha.   123   6e-25  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P18624) RecName: Full=Elongation factor 1-alpha;          Short...    79   6e-14
(O64937) RecName: Full=Elongation factor 1-alpha;          Short...    77   1e-13
FB784589_1(FB784589|pid:none) Sequence 3862 from Patent WO200803...    77   1e-13
FB784751_1(FB784751|pid:none) Sequence 4024 from Patent WO200803...    77   1e-13
BT070606_1(BT070606|pid:none) Picea sitchensis clone WS02739_H11...    77   1e-13
BT070573_1(BT070573|pid:none) Picea sitchensis clone WS02736_O15...    77   1e-13
FB784741_1(FB784741|pid:none) Sequence 4014 from Patent WO200803...    77   1e-13
EF147322_1(EF147322|pid:none) Populus trichocarpa clone WS0122_J...    77   1e-13
BT071272_1(BT071272|pid:none) Picea sitchensis clone WS02817_F13...    77   1e-13
BT071731_1(BT071731|pid:none) Picea sitchensis clone WS0443_M05 ...    77   1e-13

 psg: 0.84  gvh: 0.34  alm: 0.51  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 32.0 %: cytoplasmic
	 20.0 %: cytoskeletal

>> prediction for VFH221 is nuc

>VFH221F.Seq
TAAACAACCATGGGTAAAGAGAAAACTCACATTAACATTGTCGTCATTGGTCACGTAGAT
GCTGGTAAATCAACCACCACTGGTCATTTAATCTACAAATGNGGTGGTATCGATAAGAGA
GTTA----------