>VFH144 (VFH144Q) /CSM/VF/VFH1-B/VFH144Q.Seq.d/
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCA
GATAACACCTACAAAGTACTCGTCGATAACAAAGAGATCCAXXXXXXXXXXGGTTTCGAA
TTATGGCAAGTTAAATCNGGTTCCATCTTTAACAANATGATNGTCCCCGACTCTGTCGAA
NAAGCTAAAGATTTCTCTGAAAAGACCTTTGNTGCCAACCAANAAGCCGAAAAGAAAATG
TTTGACGACCTCGAAGCCGCCAAAGCTGAANAGAGAAAGAAAGCNGACGAAAAATTAGCN
GCCGAAAAAGCTGCTGAAAAANAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCT
GAAGAAGATTTAGTTAAAACTGATGNCAAAAAAGAAGAAGTCNAAAAATCNACCAAAAAA
GTT

nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKK
EINKVETDQLTHQYTLVISPDNTYKVLVDNKEI---

---GFELWQVKSGSIFNXMXVPDSVEXAKDFSEKTFXANQXAEKKMFDDLEAAKAEXRKK
ADEKLAAEKAAEKXAEEADEEEEEVAEEDLVKTDXKKEEVXKSTKKV

Frame A:
nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKK
EINKVETDQLTHQYTLVISPDNTYKVLVDNKEI---

---GFELWQVKSGSIFNXMXVPDSVEXAKDFSEKTFXANQXAEKKMFDDLEAAKAEXRKK
ADEKLAAEKAAEKXAEEADEEEEEVAEEDLVKTDXKKEEVXKSTKKV

Frame B:
ispiiifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllv
igikkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*r
mnkklivvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srk
kstklkpin*lintlwsfhqitptkyssitkrs---

---vsnygklnxvpsltx*xsptlsxklkislkrplxptxkpkrkclttskppklxrerk
xtkn*xpkkllkxkpkklmkkkkkslkki*lklmxkkkksknxpkk

Frame C:
flqssyl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**
la*rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke
*tks*lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqer
nqqs*nrsinssihfghftr*hlqstrr*qrdp---

---frimas*ixfhl*qxdxprlcrxs*rfl*kdlxcqpxsrkenv*rprsrqs*xekes
xrkisxrksc*kxsrrs**rrrrsr*rrfs*n*xqkrrsxkixqks

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFH144 (VFH144Q) /CSM/VF/VFH1-B/VFH144Q.Seq.d/                       1267   0.0
VFD485 (VFD485Q) /CSM/VF/VFD4-D/VFD485Q.Seq.d/                       1045   0.0
VFD121 (VFD121Q) /CSM/VF/VFD1-A/VFD121Q.Seq.d/                       1045   0.0
VFA823 (VFA823Q) /CSM/VF/VFA8-A/VFA823Q.Seq.d/                       1045   0.0
VFA820 (VFA820Q) /CSM/VF/VFA8-A/VFA820Q.Seq.d/                       1045   0.0
SLE570 (SLE570Q) /CSM/SL/SLE5-C/SLE570Q.Seq.d/                       1045   0.0
SLE160 (SLE160Q) /CSM/SL/SLE1-C/SLE160Q.Seq.d/                       1045   0.0
FC-BG06 (FC-BG06Q) /CSM/FC/FC-BG/FC-BG06Q.Seq.d/                     1045   0.0
AHA115 (AHA115Q) /CSM/AH/AHA1-A/AHA115Q.Seq.d/                       1045   0.0
AFN554 (AFN554Q) /CSM/AF/AFN5-C/AFN554Q.Seq.d/                       1045   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1037   0.0    3
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92   1e-23  3
BZ392863|BZ392863.1 EINBW38TF EI_10_12_KB Entamoeba invadens genomic clone EINBW38, DNA sequence.    52   1e-06  2
BI876472|BI876472.1 fl72h01.y1 Sugano Kawakami zebrafish DRA Danio rerio cDNA clone 3819504 5' similar to TR:Q9PUC1 Q9PUC1 CALRETICULIN. ;, mRNA sequence.    42   8e-04  2
AY342298|AY342298.1 Ictalurus punctatus ER-resident chaperone calreticulin mRNA, complete cds.    56   0.001  1
CB937139|CB937139.1 IpCGJx13_12_E07_23 IpCGJx13 Ictalurus punctatus cDNA clone IpCGJx13_12_E07 5', mRNA sequence.    56   0.001  1
BI468042|BI468042.1 EST00452 Atlantic salmon Lambda Zap Express liver cDNA library Salmo salar cDNA clone LRR7-f02 5' similar to Calreticulin, mRNA sequence.    56   0.001  1
CK420742|CK420742.1 AUF_IpTrk_27_j08 Trunk kidney cDNA library Ictalurus punctatus cDNA 5' similar to ER-resident chaperone calreticulin, mRNA sequence.    56   0.001  1
CB086220|CB086220.1 hj81f04.g1 Hedyotis centranthoides flower - Stage 2 (NYBG) Hedyotis centranthoides cDNA clone hj81f04, mRNA sequence.    36   0.001  3
AL935191|AL935191.9 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-205D20.    34   0.003  9

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   363   6e-99
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   229   1e-58
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   228   2e-58
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   228   3e-58
L27348_1(L27348|pid:none) Hordeum vulgare calreticulin (CRH1) mR...   227   4e-58
AK318734_1(AK318734|pid:none) Arabidopsis thaliana AT1G56340 mRN...   227   5e-58
U27698_1(U27698|pid:none) Arabidopsis thaliana calreticulin (AtC...   226   7e-58
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   226   9e-58
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   225   2e-57
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   224   3e-57

 psg: 1.01  gvh: 0.69  alm: 0.49  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.06  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: extracellular, including cell wall
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoplasmic
	  8.0 %: Golgi
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum

>> prediction for VFH144 is exc

>VFH144F.Seq
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCA
GATAACACCTACAAAGTACTCGTCGATAACAAAGAGATCCA----------

>VFH144Z.Seq
----------GGTTTCGAATTATGGCAAGTTAAATCNGGTTCCATCTTTAACAANATGAT
NGTCCCCGACTCTGTCGAANAAGCTAAAGATTTCTCTGAAAAGACCTTTGNTGCCAACCA
ANAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAANAGAGAAAGAA
AGCNGACGAAAAATTAGCNGCCGAAAAAGCTGCTGAAAAANAAGCCGAAGAAGCTGATGA
AGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGNCAAAAAAGAAGAAGT
CNAAAAATCNACCAAAAAAGTT

>VFH144P.Seq
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCA
GATAACACCTACAAAGTACTCGTCGATAACAAAGAGATCCA----------GGTTTCGAA
TTATGGCAAGTTAAATCNGGTTCCATCTTTAACAANATGATNGTCCCCGACTCTGTCGAA
NAAGCTAAAGATTTCTCTGAAAAGACCTTTGNTGCCAACCAANAAGCCGAAAAGAAAATG
TTTGACGACCTCGAAGCCGCCAAAGCTGAANAGAGAAAGAAAGCNGACGAAAAATTAGCN
GCCGAAAAAGCTGCTGAAAAANAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCT
GAAGAAGATTTAGTTAAAACTGATGNCAAAAAAGAAGAAGTCNAAAAATCNACCAAAAAA
GTT