>VFH125 (VFH125Q) /CSM/VF/VFH1-B/VFH125Q.Seq.d/
ATGTTCAAAATTTTTTTTAAATTACCCGTCCAAACTTCATTAGTGTCAAAATCAAGATCA
ATTCAATTTAATTATTCAACAAAAAGTAATATTAGAAATATGGAACAATACTTACCACAA
GCTAAGGAATTTATTTCATTTATTGATAAATCACCATCACCATATCATGCAGTTCAATAC
TTTTCAGAGATTTTAAAGAGTAAAGGTTTCATTCATTTATCAGAGAAACAAATGTGGGAT
ATTCAACCAAATAAAAAATATTTCTTTACTCGTAATCAATCATGTATTTCTGCTTTTGCA
GTTGGTGGTAAATATAAACCAGGTAATGGTTTTAATATTGCAGCAGCACATACTGATAGT
CCAAATTTTAAAGTTAGACCAGTATCAAAAGTTGAATCAGTTGGATATCAACAAGTTGGT
GTTGAAACATATGGTGGTGGATTATGGTATACATGGTTTGATCGTGATTTAACCGTTGCA
GGTAGAGTCATTGTTAAATCAGGTGATGGTAGTTXXXXXXXXXX

MFKIFFKLPVQTSLVSKSRSIQFNYSTKSNIRNMEQYLPQAKEFISFIDKSPSPYHAVQY
FSEILKSKGFIHLSEKQMWDIQPNKKYFFTRNQSCISAFAVGGKYKPGNGFNIAAAHTDS
PNFKVRPVSKVESVGYQQVGVETYGGGLWYTWFDRDLTVAGRVIVKSGDGS---

Frame A:
MFKIFFKLPVQTSLVSKSRSIQFNYSTKSNIRNMEQYLPQAKEFISFIDKSPSPYHAVQY
FSEILKSKGFIHLSEKQMWDIQPNKKYFFTRNQSCISAFAVGGKYKPGNGFNIAAAHTDS
PNFKVRPVSKVESVGYQQVGVETYGGGLWYTWFDRDLTVAGRVIVKSGDGS---


Frame B:
cskfflnypsklh*cqnqdqfnliiqqkvileiwnntyhklrnlfhllinhhhhimqfnt
fqrf*rvkvsfiyqrnkcgifnqiknisllvinhvflllqlvvninqvmvlilqqhiliv
qilkldqyqklnqldinklvlkhmvvdygihglivi*plqvesllnqvmv---


Frame C:
vqnff*itrpnfisvkikinsi*lfnkk*y*kygtiltts*giyfiy**ititiscssil
frdfke*rfhsfiretnvgystk*kiflys*simyfcfcsww*i*tr*wf*ycssty**s
kf*s*tsiks*iswistswc*niwwwimvymv*s*fnrcr*shc*ir*w*---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO717 (VFO717Q) /CSM/VF/VFO7-A/VFO717Q.Seq.d/                        983   0.0
VFM467 (VFM467Q) /CSM/VF/VFM4-C/VFM467Q.Seq.d/                        983   0.0
VFL626 (VFL626Q) /CSM/VF/VFL6-B/VFL626Q.Seq.d/                        983   0.0
VFK506 (VFK506Q) /CSM/VF/VFK5-A/VFK506Q.Seq.d/                        983   0.0
VFI820 (VFI820Q) /CSM/VF/VFI8-A/VFI820Q.Seq.d/                        983   0.0
VFH157 (VFH157Q) /CSM/VF/VFH1-C/VFH157Q.Seq.d/                        983   0.0
VFH125 (VFH125Q) /CSM/VF/VFH1-B/VFH125Q.Seq.d/                        983   0.0
VFD305 (VFD305Q) /CSM/VF/VFD3-A/VFD305Q.Seq.d/                        983   0.0
SLB311 (SLB311Q) /CSM/SL/SLB3-A/SLB311Q.Seq.d/                        983   0.0
SFK775 (SFK775Q) /CSM/SF/SFK7-D/SFK775Q.Seq.d/                        983   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BG525546|BG525546.1 51-5 Stevia field grown leaf cDNA Stevia rebaudiana cDNA 5', mRNAsequence.    80   4e-11  1
CB259770|CB259770.1 23-E011176-013-009-M05-T7R MPIZ-ADIS-013 Arabidopsis thaliana cDNA clone MPIZp770M059Q 5-PRIME, mRNA sequence.    64   6e-08  2
AV823138|AV823138.1 Arabidopsis thaliana cDNA clone:RAFL05-15-M22, 5'-end.    64   8e-08  2
AY133535|AY133535.1 Arabidopsis thaliana At5g60160/f15l12_20 mRNA, complete cds.    64   2e-07  2
AY085075|AY085075.1 Arabidopsis thaliana clone 126533 mRNA, complete sequence.    64   2e-07  2
BX829510|BX829510.1 Arabidopsis thaliana Full-length cDNA Complete sequence from clone  GSLTFB18ZE08 of Flowers and buds of strain col-0 of Arabidopsis  thaliana (thale cress).    64   2e-07  2
BX831354|BX831354.1 Arabidopsis thaliana Full-length cDNA Complete sequence from clone  GSLTLS80ZF04 of Adult vegetative tissue of strain col-0 of  Arabidopsis thaliana (thale cress).    64   2e-07  2
AY045584|AY045584.1 Arabidopsis thaliana AT5g60160/f15l12_20 mRNA, complete cds.    64   2e-07  2
BX831296|BX831296.1 Arabidopsis thaliana Full-length cDNA Complete sequence from clone  GSLTLS76ZD10 of Adult vegetative tissue of strain col-0 of  Arabidopsis thaliana (thale cress).    64   2e-07  2
CA523816|CA523816.1 KS12029A03 KS12 Capsicum annuum cDNA, mRNA sequence.    66   5e-07  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54M70) RecName: Full=Aspartyl aminopeptidase;          EC=3.4....   289   3e-77
BT062007_1(BT062007|pid:none) Zea mays full-length cDNA clone ZM...   165   7e-40
(Q2HJH1) RecName: Full=Aspartyl aminopeptidase;          EC=3.4....   164   2e-39
AK301919_1(AK301919|pid:none) Homo sapiens cDNA FLJ56420 complet...   163   2e-39
(Q9ULA0) RecName: Full=Aspartyl aminopeptidase;          EC=3.4....   163   2e-39
AC053503_1(AC053503|pid:none) Homo sapiens BAC clone RP11-316O14...   163   2e-39
AB168717_1(AB168717|pid:none) Macaca fascicularis testis cDNA cl...   163   2e-39
AK001777_1(AK001777|pid:none) Homo sapiens cDNA FLJ10915 fis, cl...   163   2e-39
AB463418_1(AB463418|pid:none) Synthetic construct DNA, clone: pF...   163   2e-39
AX880026_1(AX880026|pid:none) Sequence 14931 from Patent EP10746...   163   2e-39

 psg: 0.84  gvh: 0.53  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.52  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 68.0 %: mitochondrial
	 24.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: cytoplasmic

>> prediction for VFH125 is mit

>VFH125F.Seq
ATGTTCAAAATTTTTTTTAAATTACCCGTCCAAACTTCATTAGTGTCAAAATCAAGATCA
ATTCAATTTAATTATTCAACAAAAAGTAATATTAGAAATATGGAACAATACTTACCACAA
GCTAAGGAATTTATTTCATTTATTGATAAATCACCATCACCATATCATGCAGTTCAATAC
TTTTCAGAGATTTTAAAGAGTAAAGGTTTCATTCATTTATCAGAGAAACAAATGTGGGAT
ATTCAACCAAATAAAAAATATTTCTTTACTCGTAATCAATCATGTATTTCTGCTTTTGCA
GTTGGTGGTAAATATAAACCAGGTAATGGTTTTAATATTGCAGCAGCACATACTGATAGT
CCAAATTTTAAAGTTAGACCAGTATCAAAAGTTGAATCAGTTGGATATCAACAAGTTGGT
GTTGAAACATATGGTGGTGGATTATGGTATACATGGTTTGATCGTGATTTAACCGTTGCA
GGTAGAGTCATTGTTAAATCAGGTGATGGTAGTT----------